CRISPR technology is a powerful method for genome editing that allows for alterations in DNA sequences to enhance gene function. As the global population increases, there is a pressing need to double crop production by 2050, making CRISPR a preferable choice among various genome editing techniques for improving crop yields. The document outlines the applications of CRISPR across the food supply chain, addressing the challenges of limited food supply with innovative solutions.

1. Applications of CRISPR technologies
across the food supply chain

2. CRISPR technology
is a simple
yet powerful tool for
editing genomes.
It allows researchers
to easily alter
DNA sequences and
modify gene
function.
CRISPR

3. CRISPR Biology
1. CRISPR locus containing cas genes, a leader sequence, and a
repeat–spacer array
2. CRISPR adaptation involves the excision of a protospacer from invading
DNA and its incorporation as a spacer into the host’s repeat–spacer array
3. CRISPR expression involves the transcription and processing of
crRNAs (CRISPR RNAs).

4. 4. CRISPR
interference is
achieved when
Cas9 bound to the
crRNA

5. Applications of
CRISPR
Technologies

6. CRISPR Technologies Across the Food Supply Chain
Problem:
Continuously expanding world population and
Limited food supply.
Demand:
Crop production must be doubled till 2050.

7. Solution
Improvement of crops to
increase yield.
Many techniques enable such improvements,
but most researchers are turning to the
promise of genome editing.

8. Methodolo
gies
1. zinc finger nucleases
(ZFNs)
2. TALENs
3. CRISPRs
CRISPR has emerged as the clear leader among
the three technologies.