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Using LC-MS/MS and Advanced Software Tools to Screen for unknown and Non-targ...AB SCIEX India
LC-MS/MS is a powerful tool for the analysis of Pharmaceuticals and Personal Care Products in environmental samples. The combination of high resolution LC separation and high sensitivity MS/MS is the most powerful tool to screen and quantify targeted compounds.
I use this slide for my weekly presentation during my study to present the fundamental study of capillary electrophoresis. This slide also highlighted the use of ionic liquid to modify cyclodextrin as chiral selector.
Using LC-MS/MS and Advanced Software Tools to Screen for unknown and Non-targ...AB SCIEX India
LC-MS/MS is a powerful tool for the analysis of Pharmaceuticals and Personal Care Products in environmental samples. The combination of high resolution LC separation and high sensitivity MS/MS is the most powerful tool to screen and quantify targeted compounds.
I use this slide for my weekly presentation during my study to present the fundamental study of capillary electrophoresis. This slide also highlighted the use of ionic liquid to modify cyclodextrin as chiral selector.
In the past, measuring the total amount of an element was sufficient. Unfortunately, the effects of an element extend far beyond its absolute amount. Different forms of an element can exhibit very different physicochemical properties, including varying toxicities. The process of separation and quantification of different chemical forms of an element, more specifically termed speciation analysis, can be utilized to determine an element’s various chemical forms. The number of environmental applications of elemental speciation analysis has increased significantly. For example, both the United States EPA and the European Union have specified maximum admissible chromium concentrations in their respective drinking water directives and are evaluating the inclusion of hexavalent chromium in certain legislation. Learn about the latest developments in chromatography technology for speciation analysis that offer data for a wide variety of applications, including chromium in drinking water and both arsenic and sulfur in environmental waters.
An introduction to the use of ICP-MS in the clinical setting, that goes on to describe some potential new application areas for advanced instrumentation such as HPLC-ICP-MS, laser ablation-ICP-MS and immuno-tagging-ICP-MS for the measurement of biomolecules.
LCMS is hyphenated technique. During Analytical method development analyst has to face many problems like Carry over ,matrix effect, adduct formation etc. For development of reproducible and robust analytical method “Troubleshooting is very essential”.
Application of ICP-MS and LC-ICP-MS in Drug DevelopmentQPS Holdings, LLC
Inductively coupled mass spectroscopy plasma (ICP-MS) has big potential in preclinical and clinical studies of new drug candidates. One particular area is metallodrugs.
Advances in Capillary Liquid Chromatography for High-Throughput Top Down Prot...Expedeon
Top Down proteomics has benefited greatly from advances in
mass spectrometry instrumentation and database searching,
yet it has been hindered by the lack of robust separation
platforms for intact proteins. Recently, the use of Gel-Eluted
Liquid Fraction Entrapment Electrophoresis (GELFrEE)1,2
followed by capillary liquid chromatography-MS/MS has
enabled hundreds of Top Down identifications from a single
proteome run.
In the past, measuring the total amount of an element was sufficient. Unfortunately, the effects of an element extend far beyond its absolute amount. Different forms of an element can exhibit very different physicochemical properties, including varying toxicities. The process of separation and quantification of different chemical forms of an element, more specifically termed speciation analysis, can be utilized to determine an element’s various chemical forms. The number of environmental applications of elemental speciation analysis has increased significantly. For example, both the United States EPA and the European Union have specified maximum admissible chromium concentrations in their respective drinking water directives and are evaluating the inclusion of hexavalent chromium in certain legislation. Learn about the latest developments in chromatography technology for speciation analysis that offer data for a wide variety of applications, including chromium in drinking water and both arsenic and sulfur in environmental waters.
An introduction to the use of ICP-MS in the clinical setting, that goes on to describe some potential new application areas for advanced instrumentation such as HPLC-ICP-MS, laser ablation-ICP-MS and immuno-tagging-ICP-MS for the measurement of biomolecules.
LCMS is hyphenated technique. During Analytical method development analyst has to face many problems like Carry over ,matrix effect, adduct formation etc. For development of reproducible and robust analytical method “Troubleshooting is very essential”.
Application of ICP-MS and LC-ICP-MS in Drug DevelopmentQPS Holdings, LLC
Inductively coupled mass spectroscopy plasma (ICP-MS) has big potential in preclinical and clinical studies of new drug candidates. One particular area is metallodrugs.
Advances in Capillary Liquid Chromatography for High-Throughput Top Down Prot...Expedeon
Top Down proteomics has benefited greatly from advances in
mass spectrometry instrumentation and database searching,
yet it has been hindered by the lack of robust separation
platforms for intact proteins. Recently, the use of Gel-Eluted
Liquid Fraction Entrapment Electrophoresis (GELFrEE)1,2
followed by capillary liquid chromatography-MS/MS has
enabled hundreds of Top Down identifications from a single
proteome run.
Certainly! The **basic principle of coulometry** involves passing a known electrical charge through a solution containing the analyte. Coulometry can be used to determine the amount of a substance in a solution, the purity of a compound, or the kinetics of an electrochemical reaction¹[3] ²[4]. It is a valuable technique in analytical electrochemistry for precision measurements of charge and is named after Charles-Augustin de Coulomb³[2]. One useful application of coulometry is determining the number of electrons involved in a redox reaction, which can be achieved through controlled-potential coulometric analysis using a known amount of a pure compound.
It includes-
calcium ion introduction
pathophysiological role of calcium
signalling pathways for calcium release
calcium channels
assays for calcium detection
Micro Electronic Pill Seminar presentation by Vaibhav RichVaibhav Kumar
Power Point Presentation on "Micro Electronic Pill" for b,tech Seminar by Vaibhav Rich from Dr. AITH Kanpur.Best content of E-pill with different figure and Diagram.It is best one for Electronic engineering Presentation Topic.
It relate with the spectrophotometry. The components and the uses of each of them are detailed. The ultimate goal of using this spectrometry and the uses of them also explained well.
GCMS & LCMS
htps://youtube.com/vishalshelke99
https://instagram.com/vishal_stagram
Sub :- Advanced Analytical Techniques
M.Pharmacy Sem1
Savitribai Phule Pune University
Contents :-
GC-MS
Introduction
Principle
Instrumentation
Application
LC-MS
Introduction
Principle
Instrumentation
Application
Introduction to Gas chromatography-Mass spectroscopy
Gas chromatography-Mass spectroscopy is one of the so-called hyphenated analytical techniques. It is actually two techniques that are combined to form a single method of analyzing mixtures of chemicals
GC-MS is an instrumental technique, comprising a gas chromatograph coupled to a mass spectrometer by which complex mixtures of chemicals may be separated, identified & quantified. In order to a compound to be analysed by GC-MS it must be sufficiently volatile & thermally stable.
Principle :-
The Sample solution is injected into the GC inlet where it is vapourized & swept onto a chromatographic column by the carrier gas ( usually helium). The sample flows through the column & compounds comprising the mixture of interest are separated by virtue of their relative interaction with the coating of the column (stationery phase) & the carrier gas (mobile phase). The later part of the column passes through a heated transfer line & ends at the entrance to ion source where compounds eluting from the column are converted to ions
A Novel Method for Prevention of Bandwidth Distributed Denial of Service AttacksIJERD Editor
Distributed Denial of Service (DDoS) Attacks became a massive threat to the Internet. Traditional
Architecture of internet is vulnerable to the attacks like DDoS. Attacker primarily acquire his army of Zombies,
then that army will be instructed by the Attacker that when to start an attack and on whom the attack should be
done. In this paper, different techniques which are used to perform DDoS Attacks, Tools that were used to
perform Attacks and Countermeasures in order to detect the attackers and eliminate the Bandwidth Distributed
Denial of Service attacks (B-DDoS) are reviewed. DDoS Attacks were done by using various Flooding
techniques which are used in DDoS attack.
The main purpose of this paper is to design an architecture which can reduce the Bandwidth
Distributed Denial of service Attack and make the victim site or server available for the normal users by
eliminating the zombie machines. Our Primary focus of this paper is to dispute how normal machines are
turning into zombies (Bots), how attack is been initiated, DDoS attack procedure and how an organization can
save their server from being a DDoS victim. In order to present this we implemented a simulated environment
with Cisco switches, Routers, Firewall, some virtual machines and some Attack tools to display a real DDoS
attack. By using Time scheduling, Resource Limiting, System log, Access Control List and some Modular
policy Framework we stopped the attack and identified the Attacker (Bot) machines
Hearing loss is one of the most common human impairments. It is estimated that by year 2015 more
than 700 million people will suffer mild deafness. Most can be helped by hearing aid devices depending on the
severity of their hearing loss. This paper describes the implementation and characterization details of a dual
channel transmitter front end (TFE) for digital hearing aid (DHA) applications that use novel micro
electromechanical- systems (MEMS) audio transducers and ultra-low power-scalable analog-to-digital
converters (ADCs), which enable a very-low form factor, energy-efficient implementation for next-generation
DHA. The contribution of the design is the implementation of the dual channel MEMS microphones and powerscalable
ADC system.
Influence of tensile behaviour of slab on the structural Behaviour of shear c...IJERD Editor
-A composite beam is composed of a steel beam and a slab connected by means of shear connectors
like studs installed on the top flange of the steel beam to form a structure behaving monolithically. This study
analyzes the effects of the tensile behavior of the slab on the structural behavior of the shear connection like slip
stiffness and maximum shear force in composite beams subjected to hogging moment. The results show that the
shear studs located in the crack-concentration zones due to large hogging moments sustain significantly smaller
shear force and slip stiffness than the other zones. Moreover, the reduction of the slip stiffness in the shear
connection appears also to be closely related to the change in the tensile strain of rebar according to the increase
of the load. Further experimental and analytical studies shall be conducted considering variables such as the
reinforcement ratio and the arrangement of shear connectors to achieve efficient design of the shear connection
in composite beams subjected to hogging moment.
Gold prospecting using Remote Sensing ‘A case study of Sudan’IJERD Editor
Gold has been extracted from northeast Africa for more than 5000 years, and this may be the first
place where the metal was extracted. The Arabian-Nubian Shield (ANS) is an exposure of Precambrian
crystalline rocks on the flanks of the Red Sea. The crystalline rocks are mostly Neoproterozoic in age. ANS
includes the nations of Israel, Jordan. Egypt, Saudi Arabia, Sudan, Eritrea, Ethiopia, Yemen, and Somalia.
Arabian Nubian Shield Consists of juvenile continental crest that formed between 900 550 Ma, when intra
oceanic arc welded together along ophiolite decorated arc. Primary Au mineralization probably developed in
association with the growth of intra oceanic arc and evolution of back arc. Multiple episodes of deformation
have obscured the primary metallogenic setting, but at least some of the deposits preserve evidence that they
originate as sea floor massive sulphide deposits.
The Red Sea Hills Region is a vast span of rugged, harsh and inhospitable sector of the Earth with
inimical moon-like terrain, nevertheless since ancient times it is famed to be an abode of gold and was a major
source of wealth for the Pharaohs of ancient Egypt. The Pharaohs old workings have been periodically
rediscovered through time. Recent endeavours by the Geological Research Authority of Sudan led to the
discovery of a score of occurrences with gold and massive sulphide mineralizations. In the nineties of the
previous century the Geological Research Authority of Sudan (GRAS) in cooperation with BRGM utilized
satellite data of Landsat TM using spectral ratio technique to map possible mineralized zones in the Red Sea
Hills of Sudan. The outcome of the study mapped a gossan type gold mineralization. Band ratio technique was
applied to Arbaat area and a signature of alteration zone was detected. The alteration zones are commonly
associated with mineralization. The alteration zones are commonly associated with mineralization. A filed check
confirmed the existence of stock work of gold bearing quartz in the alteration zone. Another type of gold
mineralization that was discovered using remote sensing is the gold associated with metachert in the Atmur
Desert.
Reducing Corrosion Rate by Welding DesignIJERD Editor
The paper addresses the importance of welding design to prevent corrosion at steel. Welding is
used to join pipe, profiles at bridges, spindle, and a lot more part of engineering construction. The
problems happened associated with welding are common issues in these fields, especially corrosion.
Corrosion can be reduced with many methods, they are painting, controlling humidity, and also good
welding design. In the research, it can be found that reducing residual stress on the welding can be
solved in corrosion rate reduction problem.
Preheating on 500oC and 600oC give better condition to reduce corosion rate than condition after
preheating 400oC. For all welding groove type, material with 500oC and 600oC preheating after 14 days
corrosion test is 0,5%-0,69% lost. Material with 400oC preheating after 14 days corrosion test is 0,57%-0,76%
lost.
Welding groove also influence corrosion rate. X and V type welding groove give better condition to reduce
corrosion rate than use 1/2V and 1/2 X welding groove. After 14 days corrosion test, the samples with
X welding groove type is 0,5%-0,57% lost. The samples with V welding groove after 14 days corrosion test is
0,51%-0,59% lost. The samples with 1/2V and 1/2X welding groove after 14 days corrosion test is 0,58%-
0,71% lost.
Router 1X3 – RTL Design and VerificationIJERD Editor
Routing is the process of moving a packet of data from source to destination and enables messages
to pass from one computer to another and eventually reach the target machine. A router is a networking device
that forwards data packets between computer networks. It is connected to two or more data lines from different
networks (as opposed to a network switch, which connects data lines from one single network). This paper,
mainly emphasizes upon the study of router device, it‟s top level architecture, and how various sub-modules of
router i.e. Register, FIFO, FSM and Synchronizer are synthesized, and simulated and finally connected to its top
module.
Active Power Exchange in Distributed Power-Flow Controller (DPFC) At Third Ha...IJERD Editor
This paper presents a component within the flexible ac-transmission system (FACTS) family, called
distributed power-flow controller (DPFC). The DPFC is derived from the unified power-flow controller (UPFC)
with an eliminated common dc link. The DPFC has the same control capabilities as the UPFC, which comprise
the adjustment of the line impedance, the transmission angle, and the bus voltage. The active power exchange
between the shunt and series converters, which is through the common dc link in the UPFC, is now through the
transmission lines at the third-harmonic frequency. DPFC multiple small-size single-phase converters which
reduces the cost of equipment, no voltage isolation between phases, increases redundancy and there by
reliability increases. The principle and analysis of the DPFC are presented in this paper and the corresponding
simulation results that are carried out on a scaled prototype are also shown.
Mitigation of Voltage Sag/Swell with Fuzzy Control Reduced Rating DVRIJERD Editor
Power quality has been an issue that is becoming increasingly pivotal in industrial electricity
consumers point of view in recent times. Modern industries employ Sensitive power electronic equipments,
control devices and non-linear loads as part of automated processes to increase energy efficiency and
productivity. Voltage disturbances are the most common power quality problem due to this the use of a large
numbers of sophisticated and sensitive electronic equipment in industrial systems is increased. This paper
discusses the design and simulation of dynamic voltage restorer for improvement of power quality and
reduce the harmonics distortion of sensitive loads. Power quality problem is occurring at non-standard
voltage, current and frequency. Electronic devices are very sensitive loads. In power system voltage sag,
swell, flicker and harmonics are some of the problem to the sensitive load. The compensation capability
of a DVR depends primarily on the maximum voltage injection ability and the amount of stored
energy available within the restorer. This device is connected in series with the distribution feeder at
medium voltage. A fuzzy logic control is used to produce the gate pulses for control circuit of DVR and the
circuit is simulated by using MATLAB/SIMULINK software.
Study on the Fused Deposition Modelling In Additive ManufacturingIJERD Editor
Additive manufacturing process, also popularly known as 3-D printing, is a process where a product
is created in a succession of layers. It is based on a novel materials incremental manufacturing philosophy.
Unlike conventional manufacturing processes where material is removed from a given work price to derive the
final shape of a product, 3-D printing develops the product from scratch thus obviating the necessity to cut away
materials. This prevents wastage of raw materials. Commonly used raw materials for the process are ABS
plastic, PLA and nylon. Recently the use of gold, bronze and wood has also been implemented. The complexity
factor of this process is 0% as in any object of any shape and size can be manufactured.
Spyware triggering system by particular string valueIJERD Editor
This computer programme can be used for good and bad purpose in hacking or in any general
purpose. We can say it is next step for hacking techniques such as keylogger and spyware. Once in this system if
user or hacker store particular string as a input after that software continually compare typing activity of user
with that stored string and if it is match then launch spyware programme.
A Blind Steganalysis on JPEG Gray Level Image Based on Statistical Features a...IJERD Editor
This paper presents a blind steganalysis technique to effectively attack the JPEG steganographic
schemes i.e. Jsteg, F5, Outguess and DWT Based. The proposed method exploits the correlations between
block-DCTcoefficients from intra-block and inter-block relation and the statistical moments of characteristic
functions of the test image is selected as features. The features are extracted from the BDCT JPEG 2-array.
Support Vector Machine with cross-validation is implemented for the classification.The proposed scheme gives
improved outcome in attacking.
Secure Image Transmission for Cloud Storage System Using Hybrid SchemeIJERD Editor
- Data over the cloud is transferred or transmitted between servers and users. Privacy of that
data is very important as it belongs to personal information. If data get hacked by the hacker, can be
used to defame a person’s social data. Sometimes delay are held during data transmission. i.e. Mobile
communication, bandwidth is low. Hence compression algorithms are proposed for fast and efficient
transmission, encryption is used for security purposes and blurring is used by providing additional
layers of security. These algorithms are hybridized for having a robust and efficient security and
transmission over cloud storage system.
Application of Buckley-Leverett Equation in Modeling the Radius of Invasion i...IJERD Editor
A thorough review of existing literature indicates that the Buckley-Leverett equation only analyzes
waterflood practices directly without any adjustments on real reservoir scenarios. By doing so, quite a number
of errors are introduced into these analyses. Also, for most waterflood scenarios, a radial investigation is more
appropriate than a simplified linear system. This study investigates the adoption of the Buckley-Leverett
equation to estimate the radius invasion of the displacing fluid during waterflooding. The model is also adopted
for a Microbial flood and a comparative analysis is conducted for both waterflooding and microbial flooding.
Results shown from the analysis doesn’t only records a success in determining the radial distance of the leading
edge of water during the flooding process, but also gives a clearer understanding of the applicability of
microbes to enhance oil production through in-situ production of bio-products like bio surfactans, biogenic
gases, bio acids etc.
Gesture Gaming on the World Wide Web Using an Ordinary Web CameraIJERD Editor
- Gesture gaming is a method by which users having a laptop/pc/x-box play games using natural or
bodily gestures. This paper presents a way of playing free flash games on the internet using an ordinary webcam
with the help of open source technologies. Emphasis in human activity recognition is given on the pose
estimation and the consistency in the pose of the player. These are estimated with the help of an ordinary web
camera having different resolutions from VGA to 20mps. Our work involved giving a 10 second documentary to
the user on how to play a particular game using gestures and what are the various kinds of gestures that can be
performed in front of the system. The initial inputs of the RGB values for the gesture component is obtained by
instructing the user to place his component in a red box in about 10 seconds after the short documentary before
the game is finished. Later the system opens the concerned game on the internet on popular flash game sites like
miniclip, games arcade, GameStop etc and loads the game clicking at various places and brings the state to a
place where the user is to perform only gestures to start playing the game. At any point of time the user can call
off the game by hitting the esc key and the program will release all of the controls and return to the desktop. It
was noted that the results obtained using an ordinary webcam matched that of the Kinect and the users could
relive the gaming experience of the free flash games on the net. Therefore effective in game advertising could
also be achieved thus resulting in a disruptive growth to the advertising firms.
Hardware Analysis of Resonant Frequency Converter Using Isolated Circuits And...IJERD Editor
-LLC resonant frequency converter is basically a combo of series as well as parallel resonant ckt. For
LCC resonant converter it is associated with a disadvantage that, though it has two resonant frequencies, the
lower resonant frequency is in ZCS region[5]. For this application, we are not able to design the converter
working at this resonant frequency. LLC resonant converter existed for a very long time but because of
unknown characteristic of this converter it was used as a series resonant converter with basically a passive
(resistive) load. . Here, it was designed to operate in switching frequency higher than resonant frequency of the
series resonant tank of Lr and Cr converter acts very similar to Series Resonant Converter. The benefit of LLC
resonant converter is narrow switching frequency range with light load[6] . Basically, the control ckt plays a
very imp. role and hence 555 Timer used here provides a perfect square wave as the control ckt provides no
slew rate which makes the square wave really strong and impenetrable. The dead band circuit provides the
exclusive dead band in micro seconds so as to avoid the simultaneous firing of two pairs of IGBT’s where one
pair switches off and the other on for a slightest period of time. Hence, the isolator ckt here is associated with
each and every ckt used because it acts as a driver and an isolation to each of the IGBT is provided with one
exclusive transformer supply[3]. The IGBT’s are fired using the appropriate signal using the previous boards
and hence at last a high frequency rectifier ckt with a filtering capacitor is used to get an exact dc
waveform .The basic goal of this particular analysis is to observe the wave forms and characteristics of
converters with differently positioned passive elements in the form of tank circuits.
Simulated Analysis of Resonant Frequency Converter Using Different Tank Circu...IJERD Editor
LLC resonant frequency converter is basically a combo of series as well as parallel resonant ckt. For
LCC resonant converter it is associated with a disadvantage that, though it has two resonant frequencies, the
lower resonant frequency is in ZCS region [5]. For this application, we are not able to design the converter
working at this resonant frequency. LLC resonant converter existed for a very long time but because of
unknown characteristic of this converter it was used as a series resonant converter with basically a passive
(resistive) load. . Here, it was designed to operate in switching frequency higher than resonant frequency of the
series resonant tank of Lr and Cr converter acts very similar to Series Resonant Converter. The benefit of LLC
resonant converter is narrow switching frequency range with light load[6] . Basically, the control ckt plays a
very imp. role and hence 555 Timer used here provides a perfect square wave as the control ckt provides no
slew rate which makes the square wave really strong and impenetrable. The dead band circuit provides the
exclusive dead band in micro seconds so as to avoid the simultaneous firing of two pairs of IGBT’s where one
pair switches off and the other on for a slightest period of time. Hence, the isolator ckt here is associated with
each and every ckt used because it acts as a driver and an isolation to each of the IGBT is provided with one
exclusive transformer supply[3]. The IGBT’s are fired using the appropriate signal using the previous boards
and hence at last a high frequency rectifier ckt with a filtering capacitor is used to get an exact dc
waveform .The basic goal of this particular analysis is to observe the wave forms and characteristics of
converters with differently positioned passive elements in the form of tank circuits. The supported simulation
is done through PSIM 6.0 software tool
Amateurs Radio operator, also known as HAM communicates with other HAMs through Radio
waves. Wireless communication in which Moon is used as natural satellite is called Moon-bounce or EME
(Earth -Moon-Earth) technique. Long distance communication (DXing) using Very High Frequency (VHF)
operated amateur HAM radio was difficult. Even with the modest setup having good transceiver, power
amplifier and high gain antenna with high directivity, VHF DXing is possible. Generally 2X11 YAGI antenna
along with rotor to set horizontal and vertical angle is used. Moon tracking software gives exact location,
visibility of Moon at both the stations and other vital data to acquire real time position of moon.
“MS-Extractor: An Innovative Approach to Extract Microsatellites on „Y‟ Chrom...IJERD Editor
Simple Sequence Repeats (SSR), also known as Microsatellites, have been extensively used as
molecular markers due to their abundance and high degree of polymorphism. The nucleotide sequences of
polymorphic forms of the same gene should be 99.9% identical. So, Microsatellites extraction from the Gene is
crucial. However, Microsatellites repeat count is compared, if they differ largely, he has some disorder. The Y
chromosome likely contains 50 to 60 genes that provide instructions for making proteins. Because only males
have the Y chromosome, the genes on this chromosome tend to be involved in male sex determination and
development. Several Microsatellite Extractors exist and they fail to extract microsatellites on large data sets of
giga bytes and tera bytes in size. The proposed tool “MS-Extractor: An Innovative Approach to extract
Microsatellites on „Y‟ Chromosome” can extract both Perfect as well as Imperfect Microsatellites from large
data sets of human genome „Y‟. The proposed system uses string matching with sliding window approach to
locate Microsatellites and extracts them.
Importance of Measurements in Smart GridIJERD Editor
- The need to get reliable supply, independence from fossil fuels, and capability to provide clean
energy at a fixed and lower cost, the existing power grid structure is transforming into Smart Grid. The
development of a smart energy distribution grid is a current goal of many nations. A Smart Grid should have
new capabilities such as self-healing, high reliability, energy management, and real-time pricing. This new era
of smart future grid will lead to major changes in existing technologies at generation, transmission and
distribution levels. The incorporation of renewable energy resources and distribution generators in the existing
grid will increase the complexity, optimization problems and instability of the system. This will lead to a
paradigm shift in the instrumentation and control requirements for Smart Grids for high quality, stable and
reliable electricity supply of power. The monitoring of the grid system state and stability relies on the
availability of reliable measurement of data. In this paper the measurement areas that highlight new
measurement challenges, development of the Smart Meters and the critical parameters of electric energy to be
monitored for improving the reliability of power systems has been discussed.
Study of Macro level Properties of SCC using GGBS and Lime stone powderIJERD Editor
One of the major environmental concerns is the disposal of the waste materials and utilization of
industrial by products. Lime stone quarries will produce millions of tons waste dust powder every year. Having
considerable high degree of fineness in comparision to cement this material may be utilized as a partial
replacement to cement. For this purpose an experiment is conducted to investigate the possibility of using lime
stone powder in the production of SCC with combined use GGBS and how it affects the fresh and mechanical
properties of SCC. First SCC is made by replacing cement with GGBS in percentages like 10, 20, 30, 40, 50 and
by taking the optimum mix with GGBS lime stone powder is blended to mix in percentages like 5, 10, 15, 20 as
a partial replacement to cement. Test results shows that the SCC mix with combination of 30% GGBS and 15%
limestone powder gives maximum compressive strength and fresh properties are also in the limits prescribed by
the EFNARC.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
UiPath Test Automation using UiPath Test Suite series, part 3DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 3. In this session, we will cover desktop automation along with UI automation.
Topics covered:
UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
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See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
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FIDO Alliance Osaka Seminar: Passkeys and the Road Ahead.pdf
International Journal of Engineering Research and Development (IJERD)
1. International Journal of Engineering Research and Development
e-ISSN: 2278-067X, p-ISSN: 2278-800X, www.ijerd.com
Volume 7, Issue 8 (June 2013), PP. 08-11
8
Colorimetry Based Calcium Measurement
Rupali Vijay Kajalkar1
, Ashok D Gaikwad2
Abstract:- Calcium Analysis is one of the significant parameter for the clinical diagnosis. Calcium controls
osmosis and diffusion through the cell membranes, and also the passing of information within the cell. Normal
range of Calcium in human serum is around 8.5mg/dl-10.5mg/dl. The paper is focused on the Colorimetric
Sensing technique for measurement of calcium. Colorimetry is a useful analytical tool for determining
concentration of colored material in the solution. This sensing technique consists of LED as a light source of
650nm and a high speed photodiode BPW34 as a detector. Calcium reacts with ArsenazoIII and forms a colored
complex. Concentration of calcium is measured by measuring the absorbance using Beer Lambert’s law. The
paper describes the test preparation and measurement procedures. The designed colorimetric sensing system is
maintenance free and is cost effective.
Keywords:- Absorbance, Calcium, Concentration, Cuvette.
I. INTRODUCTION
Calcium is one of the most essential nutrients needed by the body for healthy functioning. Calcium
controls muscle contraction and relaxation, it is also responsible for nerve impulse transmission and the transfer
of information between our brain cells. Blood calcium test helps in diagnosing kidney stones, thyroid disorders,
intestinal disorders, bone disorders and neurological disorders. Fig.1 shows the calcium regulation in the body.
Calcium levels in the blood are regulated by parathyroid hormone. This parathyroid hormone (Pth) is secreted
by the parathyroid glands located in the neck. When blood levels of calcium drop, the parathyroid glands release
parathyroid hormone. Parathyroid hormone then stimulates the conversion of vitamin D to calcitriol in the
kidneys to increase blood levels of calcium. When blood levels of calcium are normal, the parathyroid gland
inhibits the production of parathyroid hormone. The kidneys then dispose of excess calcium via urine. Calcium
is the main buffer used in the body to neutralize acids and maintain the proper pH.
Fig. 1 Calcium Regulation
II. METHODOLOGY
A. Atomic Absorption Spectrophotometry
Atomic Absorption Spectroscopy in analytical chemistry is a technique for determining the
concentration of a particular metal element within a sample. It consists of two processes. First is the production
of free atoms from the sample and second is absorption of radiation from an external source by these atoms.
This method is not used in pathology laboratories as it has a large number of interferences such as formation of
non-volatile compounds, smoke formation which will absorb light, contamination of sample large sample
quantity required (1ml-2ml) and problems with refractory element.
2. Colorimetry Based Calcium Measurement
9
B. Ion Selective Electrode
An ion selective electrode generates a difference in electrical potential between itself and a reference
electrode. The output potential is proportional to the concentration of the selected ion in the solution. The
concentration is a measure of the number of ions in a specific volume. The electrode measures the activity rather
than the finite concentration This method is most commonly used in the laboratories for précised measurement
of all ions. Example Na, K, Ca and Cl. A dedicated electrode and reference electrode is placed in an ISE
analyzer. Cleaning of electrodes is done after every test as it causes corrosion. Electrodes are to be replaced
regularly which adds to the cost.
C. Colorimetry
Colorimetric technique is a useful analytical tool for determining concentration of coloured material in
the solution. Coloured substances absorb light in the visible spectrum and amount of light absorbed is
proportional to the concentration of substance in solution. The biochemistry analysers based on colorimetric
technique consists of tungsten halogen lamp as the light source. The lamp has to be adjusted with the filters or
monochromator to get the desired wavelength. The cost of the instrument increases.
The proposed Colorimetric sensor design consists of LED of 650nm as the light source. As the LED offers the
desired wavelength, the need of monochromator or filter is eliminated.
III. SYSTEM OVERVIEW
A. BASIC PRINCIPLE
Colorimetry works on Beer Lambert’s Law. It states that the concentration of substance is determined
by directing a beam of monochromatic light through a cuvette containing the solution and measuring amount of
light absorbed [5]. The formula is given below[5].
Absorbance α path length (l) *concentration
A = ε *l * c
ε = molar extinction coefficient.
B. BLOCK DIAGRAM
The system consists of Light intensity measurement unit and Data acquisition system. Fig2 shows the
system block diagram Light Intensity Measurement unit is composed of LED of 650nm wavelength as the light
source, a cuvette assembly and a photo detector with the signal conditioning circuitry. The output voltage is
amplified and given to the data acquisition system. Data Acquisition System is driven by microcontroller
ATmega 644. Atmega 644 is a low power 8 bit microcontroller with 64kbytes in system programmable flash
memory, inbuilt A/D converter, and 4kbytes of internal SRAM and 2kbytes of EEPROM. It consists of 8
channels, 10 bit successive approximation A/D converter.
Fig.2 Block Diagram
C. SENSOR DESIGN
The sensor design of the system is shown in the fig.3. It consists of 12v dc power supply regulated to
8v by 7808 regulator. Intensity modulation circuit drives the LED through 1k resistor. The light source used is
of 650 nm wavelength. The LED is the best light source used as compared to the incandescent bulbs as they
offer enhanced spectral purity, higher brightness, increased lifetime, reduced power consumption[1]. The
photodiode BPW34 is used as the detector and driven by the regulator. Photodiodes are semiconductor light
sensors that generate a current or voltage when illuminated by light. BPW34 is the silicon PIN photodiode with
high speed and radiant sensitivity. It has rapid response and wider range from 430nm-1100nm. It is sensitive to
visible and near infrared radiation. The output of photodiode is given to current to voltage converter. The
3. Colorimetry Based Calcium Measurement
10
voltage at the output of signal conditioning circuit is the transmitted voltage which is logarithmically converted
to get the absorbance.
Fig 3. Sensor Design
IV. SAMPLE ANALYSIS
Human Blood is centrifuged and plain serum is used for the calcium test. Calcium is highly sensitive
for arsenazoIII reagent. The calcium ions in the serum react with arsenazo to form blue purple colored complex.
It generates an absorbance peak at around 600nm- 650nm. To measure the concentration of calcium in human
serum, the amount of light transmitted is measured by measuring the voltage at the output of signal conditioning
circuit. The measured voltage is logarithmically converted to get the absorbance.
The sample analysis is done in 4 steps.
1. Distilled water Blank
2. Reagent Blank
3. Standard
4. Test
The incident light is measured by placing 1000µl of distilled water in the cuvette and voltage reading is
taken as Vblank.. Reagent blanking is done by placing 1000µl of arsenazo reagent in the cuvette and reading is
taken. The 10µl of standard solution is added to the reagent blank and reading is taken. The Vstd reading is
obtained by subtracting the reagent blank from the standard reading. The test sample is run with the same
procedure as standard. The 10µl of test sample is added to 1000µl of reagent and the reading is Vtest...Reagent
blanking procedure is done for all the test samples and standard solutions, because along with the compound
other variables also reduce the intensity of light. The concentration of the sample is calculated using the formula
[2].
V. RESULTS
The known concentrations were run on the experimental system and the readings are plotted in fig 4.
The graph is linear. The system is linear from 3mg/dl – 18md/dl and requires dilution of the sample at higher
concentration of calcium.
4. Colorimetry Based Calcium Measurement
11
Table I: Experimental Readings
.
Fig.4 Concentration acquired on experimental system with respect to standard concentration.
VI. CONCLUSION
The colorimetric sensing system is developed and tested with the known standard concentrations of
calcium. The graph of standard concentration against acquired concentrations is plotted and found to be linear.
At higher concentrations the samples need to be diluted. The cost of the system is less and maintenance cost is
negligible.
ACKNOWLEDGMENT
The authors take this opportunity to express gratitude to the Principal and the Management of
Cummins College of Engineering for Women Pune, for providing all the support to utilize all laboratory
facilities for the project.
REFERENCES
[1]. P.Neelameegam, A.Jamaludeen, A. Rajendran and R.Raghunathan, “Measurement Of Urinary Calcium
using AT89C51RD2 Microcontroller” Review Of Scientific Instruments 80, pp044704,2009.
[2]. P.Neelameegam, A.Jamaludeen, A.Rajendran and R.Raghunathan, “Prediction of Calcium
concentration in human blood serum using an Artificial Neural Network”,Measurement 44, pp312-
319,2011.
[3]. Skoog, Holler and Neiman, “Principles of Instrumental Analysis (5th edition)”pp11-17
[4]. StewartJ.,Tavener and Jane E.Thhomas-Oates, “Build your own
spectrophotometer”,www.rsc.org/Education/Eic Sept 2007
[5]. Willard, Meritt, Dean and Settle, “Instrumental Methods Of Analysis(7th edition)”pp28-37,pp159-162
[6]. Martina OToole, Dermot Diamond; Absorbance Based Light Emitting Diode Optical Sensors and
Sensing Devices, Sensors 8,2453-2479,2008.
Standard
Concentration
Acquired
Concentration
5 5.7
10 10.3
15 13.8
20 18.2