Capillary electrophoresis
•Download as PPTX, PDF•
4 likes•2,262 views
This document provides an introduction to capillary electrophoresis-photoluminescence (CE-PL) as the final project for an instrumental analysis laboratory course. It describes CE as using an electric field to separate charged particles in a capillary. CE has advantages over liquid chromatography such as high efficiency, short analysis times, and small sample/buffer volumes. The document discusses the CE apparatus, injection methods, detectors including photoluminescence, and compares CE to LC. It then provides experimental details on using CE-PL to analyze amino acid samples from a fermentation broth over 210 hours.
Report
Share
Report
Share
Recommended
HPLC
High Performance Liquid Chromatography (HPLC) is described. HPLC uses high pressure to force a mobile phase through a column at a fast rate, increasing resolution. It discusses the types of chromatography used in HPLC, including normal phase, reverse phase, ion-exchange, and size-exclusion. The instrumentation of HPLC is also summarized, including components like the pump, mixing unit, degasser, injector, column, and detector.
Capillary electrophoresis
The document discusses capillary electrophoresis (CE), including its key terminology, instrumentation, flow dynamics, and factors that affect separation efficiency such as capillary diameter, voltage, and temperature. CE uses narrow capillaries to perform high-efficiency separations of charged molecules. When an electric field is applied, electroosmotic flow and electrophoretic migration move solutes through the capillary at different rates depending on their size and charge. Precise temperature control and optimization of factors like voltage and capillary diameter are important for achieving high resolution separations.
Capillary electrophoresis final ppt.
Capillary electrophoresis is a separation technique that uses charged molecules' differential migration in response to an applied electric field. Key components include a capillary, buffers, and detectors. Molecules are separated based on their charge and size. There are several modes, including capillary zone electrophoresis which separates based on charge and size, and micellar electrokinetic capillary chromatography which uses micelles to separate charged and neutral molecules. Capillary electrophoresis provides high resolution, efficiency, and versatility in analyzing various molecules like proteins, nucleic acids, and inorganic ions.
HPLC
This document provides an overview of high performance liquid chromatography (HPLC). It discusses how HPLC refined traditional liquid chromatography by using smaller particle sizes, smaller column diameters, and high fluid pressures to provide enhanced separations over shorter periods of time. Key aspects of HPLC systems and processes are summarized, including the use of pumps to deliver mobile phases at high pressure through columns containing small stationary phase particles. Separation is achieved based on how sample components partition between the mobile and stationary phases. Various detectors are also outlined.
Column efficiency parameters
The document discusses key concepts in gas chromatography including theoretical plates, resolution, retention time, retention volume, separation factor, height equivalent to a theoretical plate (HETP), peak asymmetry, stationary phases, and considerations for choosing stationary phases. It provides definitions and equations for these terms and concepts. Examples of common stationary phase materials and their applications are also presented.
Thin layer chromatography. (TLC)
chromatography, principle, adsorbent of TLC, mobile phase of TLC, techniques in TLC, preparation of TLC plate, standards for TLC, advantages, disadvantages of TLC, Application of TLC.
Ion Exchange Chromatography and Column Chromatography
Modern Pharmaceutical Analytical Technique
Topic: Ion Exchange Chromatography and Column Chromatography
Detector of HPLC
This document discusses different types of detectors used in high performance liquid chromatography (HPLC). It describes that detectors sense the individual components as they leave the chromatography column. There are two main types of detectors: selective detectors which respond to a particular property of the solute, and universal detectors which measure differences in a bulk property between the solute and mobile phase. Examples of common detectors discussed are UV-visible, fluorescence, refractive index, light scattering, conductivity, electrochemical, mass spectrometry, and infrared detectors. Each has distinct operating principles and applications for qualitative and quantitative analysis in HPLC.
Recommended
HPLC
High Performance Liquid Chromatography (HPLC) is described. HPLC uses high pressure to force a mobile phase through a column at a fast rate, increasing resolution. It discusses the types of chromatography used in HPLC, including normal phase, reverse phase, ion-exchange, and size-exclusion. The instrumentation of HPLC is also summarized, including components like the pump, mixing unit, degasser, injector, column, and detector.
Capillary electrophoresis
The document discusses capillary electrophoresis (CE), including its key terminology, instrumentation, flow dynamics, and factors that affect separation efficiency such as capillary diameter, voltage, and temperature. CE uses narrow capillaries to perform high-efficiency separations of charged molecules. When an electric field is applied, electroosmotic flow and electrophoretic migration move solutes through the capillary at different rates depending on their size and charge. Precise temperature control and optimization of factors like voltage and capillary diameter are important for achieving high resolution separations.
Capillary electrophoresis final ppt.
Capillary electrophoresis is a separation technique that uses charged molecules' differential migration in response to an applied electric field. Key components include a capillary, buffers, and detectors. Molecules are separated based on their charge and size. There are several modes, including capillary zone electrophoresis which separates based on charge and size, and micellar electrokinetic capillary chromatography which uses micelles to separate charged and neutral molecules. Capillary electrophoresis provides high resolution, efficiency, and versatility in analyzing various molecules like proteins, nucleic acids, and inorganic ions.
HPLC
This document provides an overview of high performance liquid chromatography (HPLC). It discusses how HPLC refined traditional liquid chromatography by using smaller particle sizes, smaller column diameters, and high fluid pressures to provide enhanced separations over shorter periods of time. Key aspects of HPLC systems and processes are summarized, including the use of pumps to deliver mobile phases at high pressure through columns containing small stationary phase particles. Separation is achieved based on how sample components partition between the mobile and stationary phases. Various detectors are also outlined.
Column efficiency parameters
The document discusses key concepts in gas chromatography including theoretical plates, resolution, retention time, retention volume, separation factor, height equivalent to a theoretical plate (HETP), peak asymmetry, stationary phases, and considerations for choosing stationary phases. It provides definitions and equations for these terms and concepts. Examples of common stationary phase materials and their applications are also presented.
Thin layer chromatography. (TLC)
chromatography, principle, adsorbent of TLC, mobile phase of TLC, techniques in TLC, preparation of TLC plate, standards for TLC, advantages, disadvantages of TLC, Application of TLC.
Ion Exchange Chromatography and Column Chromatography
Modern Pharmaceutical Analytical Technique
Topic: Ion Exchange Chromatography and Column Chromatography
Detector of HPLC
This document discusses different types of detectors used in high performance liquid chromatography (HPLC). It describes that detectors sense the individual components as they leave the chromatography column. There are two main types of detectors: selective detectors which respond to a particular property of the solute, and universal detectors which measure differences in a bulk property between the solute and mobile phase. Examples of common detectors discussed are UV-visible, fluorescence, refractive index, light scattering, conductivity, electrochemical, mass spectrometry, and infrared detectors. Each has distinct operating principles and applications for qualitative and quantitative analysis in HPLC.
Chromatography
The document discusses the principles of chromatography. It describes how chromatography separates components in a mixture based on differences in their interactions with mobile and stationary phases. It discusses how Michael Tswett first demonstrated chromatography in 1903 and the key aspects of how it works. These include how retention time, partition coefficients, selectivity factors and efficiency parameters like plate number and height equivalent to a theoretical plate are used to characterize chromatographic separations.
Capillary Electrophoresis by Sachin Kuhire
This document discusses capillary electrophoresis, a technique for separating charged molecules. It can separate proteins, peptides, amino acids, nucleic acids, and other molecules. Capillary electrophoresis works by applying an electric field across a thin capillary tube, which causes different molecules to migrate through the buffer at different rates based on their charge and size. It provides high separation efficiency using only small sample volumes. The document outlines the basic components and process of capillary electrophoresis.
Theory of high performance liquid chromatography ppt
This document provides an overview of the theory of high performance liquid chromatography (HPLC). It discusses key concepts such as the retention factor (k), which is a measure of how long a compound is retained on the column. Selectivity (α) refers to the ability to distinguish between sample components, and is calculated as a ratio of the k values. Resolution (Rs) is the most important measure of separation, and depends on factors like k, α, and the number of theoretical plates (N). N is a measure of column efficiency, and the height equivalent of a theoretical plate (HETP) describes efficiency. The document outlines how these parameters can be optimized to improve separation and resolution.
DTA AND DDTA AS PER PHARMA
DIFFERENTIAL THERMAL ANALYSIS THE TECHINQUE BASED ON TEMPERATURE USED IN INDUSTRIES OR AS A LABORATORY TECHNIQUE TO CHECK THE STATUS OF SAMPLES.
CAPPILARY ELECTROPHORESIS
Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility through thin capillary tubes when an electric field is applied. Separation occurs due to differences in electrophoretic mobility which depends on an ion's charge and size. Capillary electrophoresis uses high electric fields in thin capillaries to rapidly separate molecules in minutes versus hours for traditional electrophoresis. It has applications in sequencing DNA/RNA, analyzing pharmaceuticals, and separating proteins, peptides, and other biomolecules.
HPLC Instrumentation, Pharmaceutical analysis, HPLC detectorsInstmn sem sharu(4)
This document provides an overview of liquid chromatography (LC) and high-performance liquid chromatography (HPLC). It discusses the history and development of LC from early 1900s work to the widespread use of HPLC in the 1980s. The key aspects covered include types of HPLC techniques based on mode, principle, elution, scale, and analysis. The document also explains the basic principles, instrumentation, and components of an HPLC system including the pump, columns, detectors, and how separation is achieved based on compound affinity to the stationary phase.
Gas chromatography
This document provides an overview of gas chromatography (GC). It describes the basic components and principles of how GC works, including the carrier gas, injection port, separation column in an oven, and detector. It explains that GC separates volatile organic compounds based on how they partition between the mobile gas phase and stationary liquid phase. The document also outlines different types of columns, sample preparation procedures, common detectors like the flame ionization detector, and applications of GC in fields like environmental analysis and refineries.
Capillary Electroporesis
Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility in a thin capillary tube when an electric field is applied. It allows for much faster separation than traditional electrophoresis in under 15 minutes. Capillary electrophoresis can sequence up to 750,000 nucleotides per day due to its high resolution, reproducibility, and ability to be automated. It works by applying an electric field that causes ions to migrate through the capillary at different rates depending on their size and charge, separating them into distinct bands that can then be detected.
Detectors used in HPLC
Detectors are the brain of any chromatograhic system. It help us to record the chromatogram based on certain characteristics of the analyte and help us in identifying that compound both qualitatively and quantitatively.
Gas Chromatography
Gas chromatography head points:
Invention of Chromatography
original chromatography Experiment
Common types of chromatography
Paper and Thin layer chromatography
How does chromatography work?
Theoretical Plate
gas chromatography
schematic of GC
carrier gas-supply
Injection port
sample Injection system
split/spitless Injection
sample valves
GC columns
open tubular columns
Temperature Control
Solid Support Materials
Particle size of Supports
The stationary Phase
Detection systems
Characteristics of the Ideal Detector
Flame Ionization Detectors
Thermal Conductivity Detector
Electron-capture Detectors
https://www.linkedin.com/in/preeti-choudhary-266414182/
https://www.instagram.com/chaudharypreeti1997/
https://www.facebook.com/profile.php?id=100013419194533
https://twitter.com/preetic27018281
Please like, share, comment and follow.
stay connected
If any query then contact:
chaudharypreeti1997@gmail.com
Thanking-You
Preeti Choudhary
Derivatization in HPLC & GC
This document discusses derivatization techniques used to modify compounds to make them suitable for analysis by gas chromatography (GC) and high-performance liquid chromatography (HPLC). It describes how derivatization changes functional groups to alter chemical and physical properties without changing the overall chemical structure. Common derivatization methods include silylation, alkylation, acylation, and chiral derivatization. Silylation adds silyl groups to make compounds more volatile. Alkylation and acylation reduce polarity. Chiral derivatization forms diastereomers to separate enantiomers. Derivatization can occur before or after column separation in HPLC. Pre-column derivatization is manual while post-column occurs automatically via
Super crtical fluid chromatography ppt
This document provides an overview of supercritical fluid chromatography. It defines supercritical fluid chromatography as using a material above its critical temperature and pressure as a mobile phase. The principles are similar to HPLC but use carbon dioxide as the mobile phase. Key components of SFC instrumentation include the stationary phase, pumps to control mobile phase flow, injectors, ovens, and detectors. SFC offers advantages over GC and HPLC like lower operating temperatures and high diffusion coefficients. It finds applications in chiral separations for the pharmaceutical industry.
Vandeemeter equation
The document discusses the Van Deemter equation, which relates the height equivalent to a theoretical plate (HETP) in gas chromatography to experimental parameters like particle diameter, diffusion coefficients, and flow rate. It explains that HETP is affected by eddy diffusion, longitudinal diffusion, and mass transfer between phases. The Van Deemter equation can be used to optimize chromatographic performance by identifying conditions that minimize band broadening like adjusting flow rates and using smaller stationary phase particles.
solid phase extraction method.pptx
This document discusses solid phase extraction (SPE), a popular sample preparation technique. SPE uses a solid stationary phase to isolate and concentrate analytes from liquid samples. It reduces interference levels and minimizes final sample volumes. The degree of analyte enrichment depends on the selectivity and strength of interaction between the analyte and bonded phase. Common retention mechanisms include non-polar, polar, ionic, and hydrogen bonding interactions. SPE provides flexibility, longer column lifetimes, better contaminant removal, recovery, reproducibility and sensitivity compared to liquid-liquid extraction.
sample preparation in hplc
Sample preparation is an essential part of HPLC analysis to provide a reproducible and homogenous solution suitable for injection onto the column. The goal of sample preparation is to remove interferences and ensure the sample is compatible with the HPLC method without damaging the column. Sample matrices can be organic or inorganic solids, semisolids, liquids or gases, with liquids being easiest to prepare. Solid and semisolid samples require reducing particle size through processes like blending or grinding. Filtration is also important to remove particles that could damage the column. Common pretreatment methods for liquid samples include liquid-liquid extraction and solid phase extraction, while newer techniques are used for solid samples like supercritical fluid extraction. Derivatization can improve
Gas chromatography
Gas chromatography is a technique used to separate components of a mixture. It was invented in 1901 by Russian botanist Mikhail Tswett. Key developments include John Porter Martin developing the first gas-liquid chromatograph in the 1950s. Gas chromatography-mass spectrometry allows identification of separated components. The technique works by vaporizing a sample and carrying it by a carrier gas through a column coated with a stationary phase, separating components based on how they partition between the mobile and stationary phases.
Detector used in hplc chromatogrphy by pradeep jaiswal msc part1 mithibai col...
This document summarizes different detectors used in high performance liquid chromatography (HPLC). It describes the basic components of an HPLC system and various types of detectors including UV-VIS, photodiode array, fluorescence, conductivity, refractive index, electrochemical, and evaporative light scattering detectors. It provides details on the principle, types, working, advantages, disadvantages and applications of electrochemical detectors. The document is authored by Pradeep Jaiswal for a class on HPLC detectors.
Column chromatography
This presentation contains all the topics related to column chromatography. That includes introduction, principle,apparatus, experimental aspects of column chromatography, application of column chromatography, advantage and disadvantage of column chromatography with reference.
Sampling techniques of Infrared Spectroscopy
The document discusses various techniques for sample handling and preparation in infrared spectroscopy. It describes the different methods used for sampling solids, liquids, and gases. For solids, the key techniques discussed are running solids in solution, forming solid films, the mull technique, and pressed pellet preparation using KBr. For liquids, cells made of materials like NaCl, KBr, and ThBr are used. Gas samples utilize larger cells of the same materials to compensate for lower molecule numbers. Proper sample preparation is necessary to obtain infrared spectra without interference from cell materials.
Zone Electrophoresis- Biplob Dey
Electrophoresis is a technique used to separate macromolecules like proteins, DNA, and RNA based on their size and charge. There are two main types: free electrophoresis and zone electrophoresis. Zone electrophoresis is more commonly used and separates particles into zones or bands within a stabilizing medium like a gel or paper. Different electrophoretic techniques exist like polyacrylamide gel electrophoresis and agarose gel electrophoresis which are used to separate biomolecules and help study properties like protein structure and bacterial antibiotic resistance. Electrophoresis has applications in separating complex molecule mixtures and analyzing nucleic acids.
Capillary electrophoresis principles and applications
Capillary electrophoresis is a technique used to separate charged molecules like proteins, nucleic acids, and other small molecules based on their electrophoretic mobility. It has several advantages over traditional gel electrophoresis methods like faster separation, higher resolution, and requiring only small sample volumes. In capillary electrophoresis, samples are injected into a thin, fused silica capillary tube and separated under the influence of an applied electric field based on differences in their charge and size. Key applications of capillary electrophoresis include hemoglobin electrophoresis for detecting abnormal hemoglobins, serum protein electrophoresis, and DNA sequencing. It provides a rapid, automated, and high-resolution method for analyzing biomolecules.
Capillary Electrophoresis Presentation
The document discusses capillary electrophoresis (CE), a technique for separating ions and molecules based on their charge and size. It describes several modes of CE including capillary zone electrophoresis, capillary gel electrophoresis, isotachophoresis, and electrokinetic chromatography. Applications mentioned include pharmaceutical analysis, genetic analysis, and protein characterization. The P/ACE MDQ system automates CE and is being used in the pharmaceutical industry for applications like drug analysis, pharmacokinetic profiling, and plasma protein binding studies.
More Related Content
What's hot
Chromatography
The document discusses the principles of chromatography. It describes how chromatography separates components in a mixture based on differences in their interactions with mobile and stationary phases. It discusses how Michael Tswett first demonstrated chromatography in 1903 and the key aspects of how it works. These include how retention time, partition coefficients, selectivity factors and efficiency parameters like plate number and height equivalent to a theoretical plate are used to characterize chromatographic separations.
Capillary Electrophoresis by Sachin Kuhire
This document discusses capillary electrophoresis, a technique for separating charged molecules. It can separate proteins, peptides, amino acids, nucleic acids, and other molecules. Capillary electrophoresis works by applying an electric field across a thin capillary tube, which causes different molecules to migrate through the buffer at different rates based on their charge and size. It provides high separation efficiency using only small sample volumes. The document outlines the basic components and process of capillary electrophoresis.
Theory of high performance liquid chromatography ppt
This document provides an overview of the theory of high performance liquid chromatography (HPLC). It discusses key concepts such as the retention factor (k), which is a measure of how long a compound is retained on the column. Selectivity (α) refers to the ability to distinguish between sample components, and is calculated as a ratio of the k values. Resolution (Rs) is the most important measure of separation, and depends on factors like k, α, and the number of theoretical plates (N). N is a measure of column efficiency, and the height equivalent of a theoretical plate (HETP) describes efficiency. The document outlines how these parameters can be optimized to improve separation and resolution.
DTA AND DDTA AS PER PHARMA
DIFFERENTIAL THERMAL ANALYSIS THE TECHINQUE BASED ON TEMPERATURE USED IN INDUSTRIES OR AS A LABORATORY TECHNIQUE TO CHECK THE STATUS OF SAMPLES.
CAPPILARY ELECTROPHORESIS
Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility through thin capillary tubes when an electric field is applied. Separation occurs due to differences in electrophoretic mobility which depends on an ion's charge and size. Capillary electrophoresis uses high electric fields in thin capillaries to rapidly separate molecules in minutes versus hours for traditional electrophoresis. It has applications in sequencing DNA/RNA, analyzing pharmaceuticals, and separating proteins, peptides, and other biomolecules.
HPLC Instrumentation, Pharmaceutical analysis, HPLC detectorsInstmn sem sharu(4)
This document provides an overview of liquid chromatography (LC) and high-performance liquid chromatography (HPLC). It discusses the history and development of LC from early 1900s work to the widespread use of HPLC in the 1980s. The key aspects covered include types of HPLC techniques based on mode, principle, elution, scale, and analysis. The document also explains the basic principles, instrumentation, and components of an HPLC system including the pump, columns, detectors, and how separation is achieved based on compound affinity to the stationary phase.
Gas chromatography
This document provides an overview of gas chromatography (GC). It describes the basic components and principles of how GC works, including the carrier gas, injection port, separation column in an oven, and detector. It explains that GC separates volatile organic compounds based on how they partition between the mobile gas phase and stationary liquid phase. The document also outlines different types of columns, sample preparation procedures, common detectors like the flame ionization detector, and applications of GC in fields like environmental analysis and refineries.
Capillary Electroporesis
Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility in a thin capillary tube when an electric field is applied. It allows for much faster separation than traditional electrophoresis in under 15 minutes. Capillary electrophoresis can sequence up to 750,000 nucleotides per day due to its high resolution, reproducibility, and ability to be automated. It works by applying an electric field that causes ions to migrate through the capillary at different rates depending on their size and charge, separating them into distinct bands that can then be detected.
Detectors used in HPLC
Detectors are the brain of any chromatograhic system. It help us to record the chromatogram based on certain characteristics of the analyte and help us in identifying that compound both qualitatively and quantitatively.
Gas Chromatography
Gas chromatography head points:
Invention of Chromatography
original chromatography Experiment
Common types of chromatography
Paper and Thin layer chromatography
How does chromatography work?
Theoretical Plate
gas chromatography
schematic of GC
carrier gas-supply
Injection port
sample Injection system
split/spitless Injection
sample valves
GC columns
open tubular columns
Temperature Control
Solid Support Materials
Particle size of Supports
The stationary Phase
Detection systems
Characteristics of the Ideal Detector
Flame Ionization Detectors
Thermal Conductivity Detector
Electron-capture Detectors
https://www.linkedin.com/in/preeti-choudhary-266414182/
https://www.instagram.com/chaudharypreeti1997/
https://www.facebook.com/profile.php?id=100013419194533
https://twitter.com/preetic27018281
Please like, share, comment and follow.
stay connected
If any query then contact:
chaudharypreeti1997@gmail.com
Thanking-You
Preeti Choudhary
Derivatization in HPLC & GC
This document discusses derivatization techniques used to modify compounds to make them suitable for analysis by gas chromatography (GC) and high-performance liquid chromatography (HPLC). It describes how derivatization changes functional groups to alter chemical and physical properties without changing the overall chemical structure. Common derivatization methods include silylation, alkylation, acylation, and chiral derivatization. Silylation adds silyl groups to make compounds more volatile. Alkylation and acylation reduce polarity. Chiral derivatization forms diastereomers to separate enantiomers. Derivatization can occur before or after column separation in HPLC. Pre-column derivatization is manual while post-column occurs automatically via
Super crtical fluid chromatography ppt
This document provides an overview of supercritical fluid chromatography. It defines supercritical fluid chromatography as using a material above its critical temperature and pressure as a mobile phase. The principles are similar to HPLC but use carbon dioxide as the mobile phase. Key components of SFC instrumentation include the stationary phase, pumps to control mobile phase flow, injectors, ovens, and detectors. SFC offers advantages over GC and HPLC like lower operating temperatures and high diffusion coefficients. It finds applications in chiral separations for the pharmaceutical industry.
Vandeemeter equation
The document discusses the Van Deemter equation, which relates the height equivalent to a theoretical plate (HETP) in gas chromatography to experimental parameters like particle diameter, diffusion coefficients, and flow rate. It explains that HETP is affected by eddy diffusion, longitudinal diffusion, and mass transfer between phases. The Van Deemter equation can be used to optimize chromatographic performance by identifying conditions that minimize band broadening like adjusting flow rates and using smaller stationary phase particles.
solid phase extraction method.pptx
This document discusses solid phase extraction (SPE), a popular sample preparation technique. SPE uses a solid stationary phase to isolate and concentrate analytes from liquid samples. It reduces interference levels and minimizes final sample volumes. The degree of analyte enrichment depends on the selectivity and strength of interaction between the analyte and bonded phase. Common retention mechanisms include non-polar, polar, ionic, and hydrogen bonding interactions. SPE provides flexibility, longer column lifetimes, better contaminant removal, recovery, reproducibility and sensitivity compared to liquid-liquid extraction.
sample preparation in hplc
Sample preparation is an essential part of HPLC analysis to provide a reproducible and homogenous solution suitable for injection onto the column. The goal of sample preparation is to remove interferences and ensure the sample is compatible with the HPLC method without damaging the column. Sample matrices can be organic or inorganic solids, semisolids, liquids or gases, with liquids being easiest to prepare. Solid and semisolid samples require reducing particle size through processes like blending or grinding. Filtration is also important to remove particles that could damage the column. Common pretreatment methods for liquid samples include liquid-liquid extraction and solid phase extraction, while newer techniques are used for solid samples like supercritical fluid extraction. Derivatization can improve
Gas chromatography
Gas chromatography is a technique used to separate components of a mixture. It was invented in 1901 by Russian botanist Mikhail Tswett. Key developments include John Porter Martin developing the first gas-liquid chromatograph in the 1950s. Gas chromatography-mass spectrometry allows identification of separated components. The technique works by vaporizing a sample and carrying it by a carrier gas through a column coated with a stationary phase, separating components based on how they partition between the mobile and stationary phases.
Detector used in hplc chromatogrphy by pradeep jaiswal msc part1 mithibai col...
This document summarizes different detectors used in high performance liquid chromatography (HPLC). It describes the basic components of an HPLC system and various types of detectors including UV-VIS, photodiode array, fluorescence, conductivity, refractive index, electrochemical, and evaporative light scattering detectors. It provides details on the principle, types, working, advantages, disadvantages and applications of electrochemical detectors. The document is authored by Pradeep Jaiswal for a class on HPLC detectors.
Column chromatography
This presentation contains all the topics related to column chromatography. That includes introduction, principle,apparatus, experimental aspects of column chromatography, application of column chromatography, advantage and disadvantage of column chromatography with reference.
Sampling techniques of Infrared Spectroscopy
The document discusses various techniques for sample handling and preparation in infrared spectroscopy. It describes the different methods used for sampling solids, liquids, and gases. For solids, the key techniques discussed are running solids in solution, forming solid films, the mull technique, and pressed pellet preparation using KBr. For liquids, cells made of materials like NaCl, KBr, and ThBr are used. Gas samples utilize larger cells of the same materials to compensate for lower molecule numbers. Proper sample preparation is necessary to obtain infrared spectra without interference from cell materials.
Zone Electrophoresis- Biplob Dey
Electrophoresis is a technique used to separate macromolecules like proteins, DNA, and RNA based on their size and charge. There are two main types: free electrophoresis and zone electrophoresis. Zone electrophoresis is more commonly used and separates particles into zones or bands within a stabilizing medium like a gel or paper. Different electrophoretic techniques exist like polyacrylamide gel electrophoresis and agarose gel electrophoresis which are used to separate biomolecules and help study properties like protein structure and bacterial antibiotic resistance. Electrophoresis has applications in separating complex molecule mixtures and analyzing nucleic acids.
What's hot (20)
Theory of high performance liquid chromatography ppt
Theory of high performance liquid chromatography ppt
HPLC Instrumentation, Pharmaceutical analysis, HPLC detectorsInstmn sem sharu(4)
HPLC Instrumentation, Pharmaceutical analysis, HPLC detectorsInstmn sem sharu(4)
Detector used in hplc chromatogrphy by pradeep jaiswal msc part1 mithibai col...
Detector used in hplc chromatogrphy by pradeep jaiswal msc part1 mithibai col...
Viewers also liked
Capillary electrophoresis principles and applications
Capillary electrophoresis is a technique used to separate charged molecules like proteins, nucleic acids, and other small molecules based on their electrophoretic mobility. It has several advantages over traditional gel electrophoresis methods like faster separation, higher resolution, and requiring only small sample volumes. In capillary electrophoresis, samples are injected into a thin, fused silica capillary tube and separated under the influence of an applied electric field based on differences in their charge and size. Key applications of capillary electrophoresis include hemoglobin electrophoresis for detecting abnormal hemoglobins, serum protein electrophoresis, and DNA sequencing. It provides a rapid, automated, and high-resolution method for analyzing biomolecules.
Capillary Electrophoresis Presentation
The document discusses capillary electrophoresis (CE), a technique for separating ions and molecules based on their charge and size. It describes several modes of CE including capillary zone electrophoresis, capillary gel electrophoresis, isotachophoresis, and electrokinetic chromatography. Applications mentioned include pharmaceutical analysis, genetic analysis, and protein characterization. The P/ACE MDQ system automates CE and is being used in the pharmaceutical industry for applications like drug analysis, pharmacokinetic profiling, and plasma protein binding studies.
CAPILLARY ELECTROPHORESIS
This presention covers the working principles,the technical stories behind the scenes, merits and dmerits over conventional ways of separation.
Electrophoresis ppt.
This document provides information about electrophoresis. It discusses different types of electrophoretic techniques including slab electrophoresis, capillary electrophoresis, capillary zone electrophoresis, capillary gel electrophoresis, capillary isotachophoresis, and micellar electrokinetic chromatography. It also covers principles, instrumentation, applications in areas like DNA analysis and vaccine analysis.
Capillary electrophoresis and application by Dr. Anurag Yadav
Dr. Anurag Yadav presented on capillary electrophoresis. Capillary electrophoresis is a technique used to separate molecules like amino acids, peptides, proteins, DNA fragments, and drugs based on their charge and size. It involves applying a high voltage to a thin capillary filled with buffer, causing molecules to separate as they migrate through the capillary at different speeds. Detection is usually done through ultraviolet absorption, refractive index changes, or fluorescence near the end of the capillary. Capillary electrophoresis provides high resolution, requires only small sample volumes, and can separate a wide range of biomolecules.
Electrophoresis part 3
This document discusses two electrophoresis techniques: capillary electrophoresis and isoelectric focusing. Capillary electrophoresis uses very small volumes of samples and reagents in fused silica capillaries, allowing for high resolution, automation, and detection of low analyte levels. Isoelectric focusing separates proteins based on their isoelectric point, where they have no net charge and become immobilized in a pH gradient gel. Both techniques have various applications in biochemistry including separating proteins, DNA, amino acids, and other biomolecules.
Electrophoresis presentation
Electrophoresis is a technique used to separate charged molecules like proteins and nucleic acids based on their size and charge. It works by applying an electric current to move these molecules through a medium like a gel or paper. The document discusses different types of electrophoresis like gel electrophoresis, paper electrophoresis, and isolectric focusing. It also explains how various factors like the molecule's charge, size, and shape affect its movement during electrophoresis.
capillary electrophorosis techniques
This document provides an overview of capillary electrophoresis. It defines key terminology used in capillary electrophoresis such as migration time, electrophoretic mobility, electroosmotic flow, efficiency, and resolution. It also describes the main modes of capillary electrophoresis including capillary zone electrophoresis, isoelectric focusing, capillary gel electrophoresis, and micellar electrokinetic capillary chromatography. For each mode, it provides a brief explanation of how separation is achieved. The document concludes by noting that capillary electrophoresis techniques can be used to separate a variety of analytes including proteins, peptides, inorganic ions, and pharmaceutical compounds.
Capillary Electrophoresis
CE is an efficient separation technique that uses differences in charge and size to separate molecules in a capillary tube under the influence of an electric field. It provides high resolution separations using small sample volumes in short time frames. Variations include CZE which relies on differences in charge, CGE which separates by size in a gel, and CIEF which focuses molecules at their isoelectric point. CE has advantages over HPLC in speed, efficiency, and cost but lacks reproducibility and is not suitable for large-scale separations.
Electrophoresis and its application
Electrophoresis:
Electrophoresis is separation technique based on movement of charge particle in an electric field.
Movement of charge particles can be determined by following formula--
V= Eq/f
Where,
V= Velocity of the charged particle;
E= electric field of the molecule;
q= Net charge of the molecule; and
f= Frictional co-efficient of the molecule
Types of electrophoresis:
1. Agarose gel electrophoresis ;
2. Poly-acryl amide gel electrophoresis [PAGE];
3. Sodium do-decyl sulphate Poly- acrylamide gel electrophoresis [SDS-PAGE] ;
4. Two dimensional –Poly-acrylamide gel electrophoresis [2D-PAGE];
5. Pulse field gel electrophoresis [PFGE];
6. Capillary gel electrophoresis [CGE]; and
7. Disc electrophoresis for Protein.
Application of electrophoresis:
1. Estimation of the DNA molecule.[ Agarose , PAGE ]
2. Analysis of PCR product. [ Agarose ]
3. Separation of restricted genomic DNA and RNA. [Agarose and PAGE respectively]
4. Conformation of newly isolated DNA .[Agarose]
5. Separation of most small fragments of DNA. [PAGE]
6. In forensic science.[Agarose , PAGE, SDS-PAGE, 2D PAGE ,Capillary gel electrophoresis , PFGE]
8. In determining molecular wt. of protein.[SDS-PAGE].etc
PROTEIN ANALYSIS
PROTEIN ANALYSIS
SDS-PAGE (SDS-polyacrylamide gel electrophoresis)
Isoelectric focussing
High-performance liquid chromatography (HPLC)
Thin-layer chromatography (TLC)
Two-dimensional (2-D) gel electrophoresis
Electrophoresis
This document discusses electrophoresis, which separates charged compounds using an electric field. It moves negatively charged proteins in serum towards the anode at pH 8.6. Applications include separating serum proteins, lipoproteins, hemoglobin variants, and isoenzymes. Zone electrophoresis is commonly used and involves applying samples to a support medium like paper, cellulose acetate, or polyacrylamide gel. After separation, proteins are visualized through staining and quantified using densitometry. Serum protein electrophoresis is used to analyze the albumin and globulin percentages. Abnormal patterns can indicate conditions like multiple myeloma or liver cirrhosis.
Electrophoresis
This document provides an overview of electrophoresis techniques. It discusses the basic principles of electrophoresis, including that the rate of migration depends on the charge-to-mass ratio of particles. It describes various electrophoretic techniques such as moving boundary electrophoresis, zone electrophoresis using free solution, gel, or paper methods, as well as isoelectric focusing, isotachophoresis, and capillary electrophoresis. It provides details on how each technique is performed and discusses their applications and advantages.
Instrumental analysis
This document provides an overview of common instrumental analytical techniques used in chromatography and spectroscopy. It describes various chromatographic techniques including thin layer chromatography, column chromatography, gas chromatography, and high performance liquid chromatography. It also outlines several spectroscopic techniques such as atomic absorption spectroscopy, colorimetry, and UV-visible spectroscopy. The document explains the basic principles, components, and applications of these analytical methods for separating and analyzing mixtures.
Electrophoresis
Electrophoresis is the migration of charged particles through an electric field towards the electrode of opposite charge. It works by separating molecules based on their size and charge. Key factors that affect electrophoresis include the net charge and size/shape of molecules, as well as the ionic strength, pH, temperature, and type of support medium used. Common applications include separating proteins, nucleic acids, and other biological molecules to analyze samples.
electrophoresis
This document summarizes the principles and techniques of electrophoresis. Electrophoresis involves applying an electric field to move charged biomolecules like proteins and nucleic acids through a gel or liquid medium. It was first developed in the 1930s to study serum proteins. Factors like molecular charge, size, shape and buffer conditions determine electrophoretic mobility. Common applications include protein/nucleic acid analysis and purification. Techniques include agarose gel electrophoresis for DNA/RNA separation by size, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to denature and linearly separate proteins by mass. Isoelectric focusing separates proteins based on their isoelectric point.
Gel electrophoresis
The document provides information on gel electrophoresis techniques. It discusses different types of gels like agarose, polyacrylamide, and starch that can be used for electrophoresis. Agarose gel is commonly used to separate macromolecules like nucleic acids and proteins due to its large pore size. Polyacrylamide gel is used to separate smaller molecules and provides better resolution than agarose. SDS-PAGE allows separation based on molecular weight by making all proteins negatively charged. The document outlines the procedures for agarose and polyacrylamide gel electrophoresis and their applications in analyzing proteins and nucleic acids.
Gel electrophoresis
Gel electrophoresis is a process that uses an electric current to separate DNA fragments of varying sizes placed in an agarose gel. Smaller DNA fragments move faster and further through the gel than larger fragments, allowing separation. After running the current through the gel, DNA bands can be visualized under UV light and photographed. Gel electrophoresis revolutionized science by providing an easy way to test and compare DNA samples, leading to more advanced DNA testing with applications in forensics, paternity testing, and criminal investigations.
Separation Techniques
A pure substance consists of only one substance, while a mixture contains two or more substances not chemically combined. The purity of a substance can be determined by testing its fixed melting and boiling points or using chromatography. Various separation methods exist that separate substances based on differences in their physical properties such as solubility, boiling points, magnetism, and ability to sublime. Common separation methods include filtration, crystallization, distillation, chromatography, and magnetic attraction.
Gel electrophoresis power point
Gel electrophoresis is a process that separates DNA fragments by size. It involves placing DNA into a gel and applying an electric current, which causes the smaller fragments to travel farther through the gel than larger fragments. Gel electrophoresis is used for various applications like solving criminal cases, diagnosing genetic diseases, and determining genetic relationships between species.
Viewers also liked (20)
Capillary electrophoresis principles and applications
Capillary electrophoresis principles and applications
Capillary electrophoresis and application by Dr. Anurag Yadav
Capillary electrophoresis and application by Dr. Anurag Yadav
Similar to Capillary electrophoresis
Capillary electrophoresis
Capillary electrophoresis is a separation technique that uses narrow bore capillaries. Charged molecules migrate through the capillary under the influence of an applied electric field and separate based on their charge and size. The principle involves electrostatic forces moving molecules toward the electrode of opposite charge, as well as electroosmotic flow dragging buffer molecules. Capillary electrophoresis has various modes of operation and is used to separate and analyze biological samples in clinical and diagnostic applications.
ce ms.pptx
This document provides an overview of capillary electrophoresis-mass spectrometry (CE-MS). CE-MS combines the separation capabilities of capillary electrophoresis with the detection capabilities of mass spectrometry. Key points discussed include the instrumentation of CE-MS systems, including the capillary, high voltage supply, electrodes, detectors, mass spectrometry interface, and mass analyzer. Optimization parameters for CE-MS analyses and various applications are also summarized, such as clinical analysis of body fluids for disease biomarkers and metabolomics studies.
TECHNIQUES OF ELECTROPHORESIS
CONTENT
Capillary Electrophoresis
1) INSTRUMENTATION
2) APPLICATION
High voltage electrophoresis
1) INSTRUMENTATION
2) APPLICATION
Low voltage electrophoresis
1) INSTRUMENTATION
2) APPLICATION
Microchip electrophoresis
Microchip Electrophoresis is the new talk of the town, which revolutionize the field of electrophoresis. It is shown to be an attractive tool for time & cost saving development of a separation method for complex sample mixtures. It made possible the simultaneous separation of catecholamines and their cationic metabolites.
The principle and performance of capillary electrophoresis
Capillary electrophoresis is a technique that uses thin capillary tubes to separate ionic and molecular species based on their electrophoretic mobility. Key aspects of the technique include the use of an electric field to drive electrophoretic separation and electroosmotic flow, which allows both cations and anions to be separated in a single run. Common modes of capillary electrophoresis are capillary zone electrophoresis, capillary gel electrophoresis, capillary isoelectric focusing, and micellar electrokinetic chromatography. The document provides detailed explanations of the principles and instrumentation of capillary electrophoresis.
CP Capillary electrophoresis (1).pdf
This document provides an overview of electrophoresis and capillary electrophoresis. It defines electrophoresis as the differential movement of ions under an electric field based on their charge and size. Capillary electrophoresis separates ions in a capillary based on their electrophoretic mobility under an applied voltage. It discusses the principles, instrumentation, sample injection methods, detection methods, modes, and applications of capillary electrophoresis including genetic analysis, pharmaceutical analysis, and enantiomer separation.
Capillary Electrophoresis
This document provides an overview of electrophoresis and capillary electrophoresis. It defines electrophoresis as the differential movement of ions under an electric field based on their charge and size. Capillary electrophoresis separates ions in a capillary based on their electrophoretic mobility under an applied voltage. It discusses the principles, instrumentation, sample injection methods, detection methods, modes such as CZE and CGE, and applications for analyzing pharmaceuticals, proteins, DNA, and enantiomers. Advantages include high efficiency, speed, and automation, while disadvantages include sensitivity issues and lack of standardized methods.
Lc nmr
This document provides an overview of LC-NMR spectroscopy and its applications. It discusses the history, principles, instrumentation, and modes of LC-NMR. The key components of an LC-NMR system include the LC unit, interface, and NMR unit. Modes of analysis include continuous flow, stopped flow, time slicing, peak parking, and peak trapping. Technology such as online SPE, high field magnets, and solvent suppression methods can improve sensitivity. LC-NMR is useful for structural elucidation of organic compounds.
Sub-Percent Electron Polarimetry for the EIC
Discussion of the opportunities for precision electron beam polarimetry at the Electron-Ion Collider. Delivered at the International Workshop on Accelerator Science and Technology at Jefferson Lab on March 19, 2014.
Capillary electrophoresis.pptx
Capillary electrophoresis is an analytical technique that separates charged particles using electricity and a very thin glass tube called a capillary. It works by applying a high voltage which causes particles to separate based on their size and charge as they migrate through the capillary at different speeds. The detector measures the particles as they pass by and generates an electropherogram plot showing the separation. Capillary electrophoresis provides fast, high resolution separation and is used in applications like DNA analysis, pharmaceutical quality control, and protein characterization.
Electrophoresis presentation
This presentation provides an overview of electrophoresis techniques. It defines electrophoresis as a separation technique where solutes migrate through a conductive medium under an applied electric field. It describes how charges migrate based on their size and charge. It then discusses the different forms of electrophoresis, focusing on capillary zone electrophoresis and gel electrophoresis. For capillary electrophoresis, it explains the concepts of electrophoretic mobility, electroosmotic mobility, and their roles in solute migration. It also outlines the basic instrumentation and processes involved like injection, separation, and detection. For gel electrophoresis, it discusses how it separates biomolecules like DNA and proteins based on size and provides examples of its applications.
Capillary electrophoresis
Capillary electrophoresis is a technique that uses narrow bore capillaries to separate charged molecules via electrophoretic mobility. When a voltage is applied, molecules migrate through the capillary at different rates depending on their charge and size. This allows analytes like proteins, nucleic acids, and small molecules to be separated. Key advantages are high efficiency, short analysis times, and low sample volume requirements. Common modes include capillary zone electrophoresis, capillary gel electrophoresis, and micellar electrokinetic capillary chromatography. Applications include analysis in pharmaceuticals and detection of microbial contamination.
Methods in Electrochemical in chemistry
This document discusses various electrochemical methods and their applications. It covers topics like potentiometry, types of electrochemical cells, reference electrodes, quantitative applications in clinical and environmental analysis, and potentiometric titrations. Ion selective electrodes are explained in detail, including how they work, types of analytes and electrodes, maintenance, and applications in various fields like analytical chemistry, clinical diagnostics, and environmental monitoring. Different types of voltammetry techniques are also introduced.
Electrophoresis
Electrophoresis: introduction, definition, principal, types, apparatus, working, applictions, advantages and disadvantages.
The principle and performance of capillary electrophoresis
Capillary electrophoresis is a separation technique performed in narrow capillaries using high voltages and electric fields. It has high efficiency, requires small sample volumes, and operates quickly. Components migrate based on their electrophoretic mobility and electroosmotic flow. There are various modes that provide different selectivity, including capillary zone electrophoresis, capillary gel electrophoresis, micellar electrokinetic chromatography, and capillary electrochromatography. Capillary electrophoresis has numerous applications in biochemistry, pharmacology, toxicology, clinical chemistry, and forensics.
[Group 5] electrochemistry, electrophoresis, isoelectric focusing
This document discusses various principles of electrochemistry including concentration measurement using the Nernst equation, reference electrodes such as silver-silver chloride and calomel electrodes, indicator electrodes that are selective for specific ions, and ion-selective electrodes for measuring ions like hydrogen, sodium, and ammonium. It also covers principles of electrophoresis such as particle migration in an electric field, gel electrophoresis using agarose or polyacrylamide gels, and two-dimensional electrophoresis. Factors that affect electrochemical measurements and applications in clinical analysis are summarized.
2103313001_dhavalkumar H rathod_MPH101T.pptx
The document discusses moving boundary electrophoresis and isoelectric focusing. It begins with an introduction to electrophoresis, defines key terms, and describes the principles and factors that affect electrophoresis. It then explains moving boundary electrophoresis, which separates molecules in solution without a supporting medium, and isoelectric focusing, which establishes a pH gradient to separate proteins by their isoelectric points. The document concludes by discussing applications and disadvantages of these techniques.
Electrophoresis
Electrophoresis is a physical method used to separate charged particles like proteins and amino acids using an electric field. It works by applying a voltage across a medium like paper or gel containing the sample, causing charged molecules to migrate at different rates depending on factors like their size, shape, and charge. The history of electrophoresis began in 1931 with Arne Tiselius' work separating proteins. It continues to be developed today and has applications in biochemistry like separating amino acids, proteins, enzymes, and antibiotics.
electrophoresis-170820132815-converted.pptx
Electrophoresis is a technique used to separate charged molecules like proteins and nucleic acids based on their size and charge. It works by applying an electric current to move these molecules through a medium like a gel or paper. There are several types of electrophoresis that differ in the medium used, including gel electrophoresis, the most common, which uses an agarose or polyacrylamide gel to separate molecules by size. SDS-PAGE is a type of gel electrophoresis often used to separate proteins by molecular weight. Electrophoresis is widely applied in biomedical research and clinical diagnostics.
3.1 Test Methodologies Notes
This content is suitable for medical technologists/technicians/lab assistants/scientists writing the SMLTSA board exam. The content is also suitable for biomedical technology students and people also interested in learning about test methodologies used in medical technology. This chapter describes test methodologies and their uses. Please note that these notes are a collection I used to study for my board exam and train others who got distinctions using these.
Disclaimer: Credit goes to those who wrote the notes and the examiners of each exam question. Please use only as a reference guide and use your prescribed textbook for the latest and most accurate notes and ranges. The material here is not referenced as it is a collection of pieces of study notes from multiple people, and thus will not be held viable for any misinterpretations. Please use at your own discretion.
Similar to Capillary electrophoresis (20)
The principle and performance of capillary electrophoresis
The principle and performance of capillary electrophoresis
The principle and performance of capillary electrophoresis
The principle and performance of capillary electrophoresis
[Group 5] electrochemistry, electrophoresis, isoelectric focusing
[Group 5] electrochemistry, electrophoresis, isoelectric focusing
Recently uploaded
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
বাংলাদেশের অর্থনৈতিক সমীক্ষা ২০২৪ [Bangladesh Economic Review 2024 Bangla.pdf] কম্পিউটার , ট্যাব ও স্মার্ট ফোন ভার্সন সহ সম্পূর্ণ বাংলা ই-বুক বা pdf বই " সুচিপত্র ...বুকমার্ক মেনু 🔖 ও হাইপার লিংক মেনু 📝👆 যুক্ত ..
আমাদের সবার জন্য খুব খুব গুরুত্বপূর্ণ একটি বই ..বিসিএস, ব্যাংক, ইউনিভার্সিটি ভর্তি ও যে কোন প্রতিযোগিতা মূলক পরীক্ষার জন্য এর খুব ইম্পরট্যান্ট একটি বিষয় ...তাছাড়া বাংলাদেশের সাম্প্রতিক যে কোন ডাটা বা তথ্য এই বইতে পাবেন ...
তাই একজন নাগরিক হিসাবে এই তথ্য গুলো আপনার জানা প্রয়োজন ...।
বিসিএস ও ব্যাংক এর লিখিত পরীক্ষা ...+এছাড়া মাধ্যমিক ও উচ্চমাধ্যমিকের স্টুডেন্টদের জন্য অনেক কাজে আসবে ...
Mule event processing models | MuleSoft Mysore Meetup #47
Mule event processing models | MuleSoft Mysore Meetup #47
Event Link:- https://meetups.mulesoft.com/events/details/mulesoft-mysore-presents-mule-event-processing-models/
Agenda
● What is event processing in MuleSoft?
● Types of event processing models in Mule 4
● Distinction between the reactive, parallel, blocking & non-blocking processing
For Upcoming Meetups Join Mysore Meetup Group - https://meetups.mulesoft.com/mysore/YouTube:- youtube.com/@mulesoftmysore
Mysore WhatsApp group:- https://chat.whatsapp.com/EhqtHtCC75vCAX7gaO842N
Speaker:-
Shivani Yasaswi - https://www.linkedin.com/in/shivaniyasaswi/
Organizers:-
Shubham Chaurasia - https://www.linkedin.com/in/shubhamchaurasia1/
Giridhar Meka - https://www.linkedin.com/in/giridharmeka
Priya Shaw - https://www.linkedin.com/in/priya-shaw
BÀI TẬP BỔ TRỢ TIẾNG ANH 8 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2023-2024 (CÓ FI...
BÀI TẬP BỔ TRỢ TIẾNG ANH 8 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2023-2024 (CÓ FI...Nguyen Thanh Tu Collection
https://app.box.com/s/y977uz6bpd3af4qsebv7r9b7s21935vdMain Java[All of the Base Concepts}.docx
This is part 1 of my Java Learning Journey. This Contains Custom methods, classes, constructors, packages, multithreading , try- catch block, finally block and more.
ANATOMY AND BIOMECHANICS OF HIP JOINT.pdf
it describes the bony anatomy including the femoral head , acetabulum, labrum . also discusses the capsule , ligaments . muscle that act on the hip joint and the range of motion are outlined. factors affecting hip joint stability and weight transmission through the joint are summarized.
RHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem students
Physical pharmaceutics notes for B.pharm students
Philippine Edukasyong Pantahanan at Pangkabuhayan (EPP) Curriculum
(𝐓𝐋𝐄 𝟏𝟎𝟎) (𝐋𝐞𝐬𝐬𝐨𝐧 𝟏)-𝐏𝐫𝐞𝐥𝐢𝐦𝐬
𝐃𝐢𝐬𝐜𝐮𝐬𝐬 𝐭𝐡𝐞 𝐄𝐏𝐏 𝐂𝐮𝐫𝐫𝐢𝐜𝐮𝐥𝐮𝐦 𝐢𝐧 𝐭𝐡𝐞 𝐏𝐡𝐢𝐥𝐢𝐩𝐩𝐢𝐧𝐞𝐬:
- Understand the goals and objectives of the Edukasyong Pantahanan at Pangkabuhayan (EPP) curriculum, recognizing its importance in fostering practical life skills and values among students. Students will also be able to identify the key components and subjects covered, such as agriculture, home economics, industrial arts, and information and communication technology.
𝐄𝐱𝐩𝐥𝐚𝐢𝐧 𝐭𝐡𝐞 𝐍𝐚𝐭𝐮𝐫𝐞 𝐚𝐧𝐝 𝐒𝐜𝐨𝐩𝐞 𝐨𝐟 𝐚𝐧 𝐄𝐧𝐭𝐫𝐞𝐩𝐫𝐞𝐧𝐞𝐮𝐫:
-Define entrepreneurship, distinguishing it from general business activities by emphasizing its focus on innovation, risk-taking, and value creation. Students will describe the characteristics and traits of successful entrepreneurs, including their roles and responsibilities, and discuss the broader economic and social impacts of entrepreneurial activities on both local and global scales.
Leveraging Generative AI to Drive Nonprofit Innovation
In this webinar, participants learned how to utilize Generative AI to streamline operations and elevate member engagement. Amazon Web Service experts provided a customer specific use cases and dived into low/no-code tools that are quick and easy to deploy through Amazon Web Service (AWS.)
Gender and Mental Health - Counselling and Family Therapy Applications and In...
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
Constructing Your Course Container for Effective Communication
Communicating effectively and consistently with students can help them feel at ease during their learning experience and provide the instructor with a communication trail to track the course's progress. This workshop will take you through constructing an engaging course container to facilitate effective communication.
Advanced Java[Extra Concepts, Not Difficult].docx
This is part 2 of my Java Learning Journey. This contains Hashing, ArrayList, LinkedList, Date and Time Classes, Calendar Class and more.
BÀI TẬP DẠY THÊM TIẾNG ANH LỚP 7 CẢ NĂM FRIENDS PLUS SÁCH CHÂN TRỜI SÁNG TẠO ...
BÀI TẬP DẠY THÊM TIẾNG ANH LỚP 7 CẢ NĂM FRIENDS PLUS SÁCH CHÂN TRỜI SÁNG TẠO ...Nguyen Thanh Tu Collection
https://app.box.com/s/qhtvq32h4ybf9t49ku85x0n3xl4jhr15BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...Nguyen Thanh Tu Collection
https://app.box.com/s/tacvl9ekroe9hqupdnjruiypvm9rdanePengantar Penggunaan Flutter - Dart programming language1.pptx
Pengantar Penggunaan Flutter - Dart programming language1.pptx
Recently uploaded (20)
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
Film vocab for eal 3 students: Australia the movie
Film vocab for eal 3 students: Australia the movie
Mule event processing models | MuleSoft Mysore Meetup #47
Mule event processing models | MuleSoft Mysore Meetup #47
BÀI TẬP BỔ TRỢ TIẾNG ANH 8 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2023-2024 (CÓ FI...
BÀI TẬP BỔ TRỢ TIẾNG ANH 8 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2023-2024 (CÓ FI...
RHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem students
RHEOLOGY Physical pharmaceutics-II notes for B.pharm 4th sem students
Philippine Edukasyong Pantahanan at Pangkabuhayan (EPP) Curriculum
Philippine Edukasyong Pantahanan at Pangkabuhayan (EPP) Curriculum
Leveraging Generative AI to Drive Nonprofit Innovation
Leveraging Generative AI to Drive Nonprofit Innovation
Gender and Mental Health - Counselling and Family Therapy Applications and In...
Gender and Mental Health - Counselling and Family Therapy Applications and In...
B. Ed Syllabus for babasaheb ambedkar education university.pdf
B. Ed Syllabus for babasaheb ambedkar education university.pdf
Constructing Your Course Container for Effective Communication
Constructing Your Course Container for Effective Communication
BÀI TẬP DẠY THÊM TIẾNG ANH LỚP 7 CẢ NĂM FRIENDS PLUS SÁCH CHÂN TRỜI SÁNG TẠO ...
BÀI TẬP DẠY THÊM TIẾNG ANH LỚP 7 CẢ NĂM FRIENDS PLUS SÁCH CHÂN TRỜI SÁNG TẠO ...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...
IGCSE Biology Chapter 14- Reproduction in Plants.pdf
IGCSE Biology Chapter 14- Reproduction in Plants.pdf
Pengantar Penggunaan Flutter - Dart programming language1.pptx
Pengantar Penggunaan Flutter - Dart programming language1.pptx
Capillary electrophoresis
- 1. Instrumental Analysis Laboratory Fall 2014 Final Project 1
- 2. Introduction to CE-PL Capillary Electrophoresis-Photoluminescence 2
- 3. Capillary Electrophoresis Abbreviated as: CE: Capillary Electrophoresis CZE: Capillary Zone Electrophoresis HPCE: High-Performance Capillary Electrophoresis 3
- 4. ELECTROPHORESIS: Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a spatially uniform electric field 4
- 6. Capillary Structure and Electroosmotic Flow: 6
- 7. Injection: 7 1. Electrokinetic Method(Used for Ionic Solutes) 2. Hydrodynamic Methods(Used for Neutral Solutes) • Using Gravity Flow • Negative Pressure to the Outlet end of Column • Positive Pressure to the Sample Solution
- 8. Detector: 8 • UV-vis Absorbance • Photoluminescence • Electrochemical Methods • Mass Spectrometry • Potentiometric • Conductometric • Amperometric • Laser Induced Fluorescence(LIF) the most sensitive detection method with detection limits about nM • Indirect LIF
- 9. CE vs. LC 9 • High Efficiency • Short Analysis Time • Simple Apparatus • Ease in Changing the Separation Buffer • Small Sample and Separation Buffer Volumes • High Selectivity(through changing the buffer additives)
- 10. 10
- 11. 11 Oregongreen 488 carboxylic acid, succinimidyl ester(OG-SE) Most of the analytes do not have native fluorescence, therefor, fluorescent probe molecules should attach to the molecules
- 12. Experimental: Samples were collected every hour from fermentation broth.(for 210h) 12 Electropherogram of one of the samples ` The buffer was prepared from sodium tetraborate To enhance the separation between amino acids sodium dodecyl sulfate(SDS) and 18-crown-6 ether was added
- 13. 13 Results:
- 14. 14 Thank You For Your Consideration Ali Amiri Naeini