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1 
Atomic Absorption SSppeeccttrroossccooppyy 
(( AAAASS)) 
APPLICATIONS 
By: 
Tapeshwar Bhardwaj
2 
AAttoommiicc AAbbssoorrppttiioonn 
SSppeeccttrroossccooppyy 
(( AAAASS )) 
· IInnttrroodduuccttiioonn 
· IInnssttrruummeennttaattiioonn 
· AApppplliiccaattiioonnss
3 
Innttrroodduuccttiioonn 
What is AAS ? 
Atomic Absorption Spectroscopy is 
a quantitative method of analysis 
that is applicable to many metals 
and a few elements.
4 
What is AAS ? 
Introduced in 1955 
By Alan Walsh in Australia 
First commercial AA Spectrometer 
was introduced in 1955. 
Alan Walsh 1916-1998
5 
What is AAS ? 
• An atomic absorption spectrophotometer 
consists of a light source, a sample 
compartment and a detector. 
SSaammppllee 
CCoommppaarrttmmeenntt 
LLiigghhtt SSoouurrccee DDeetteeccttoorr
6 
IInnssttrruummeennttaattiioonn 
Line 
source Atomization 
Monochromator Detector 
Nebulizer Read-out 
Schematic diagram of a flame 
spectrophotomer
7 
HHoollllooww CCaatthhooddee LLaammpp ((HHCCLL)) 
Cathode--- in the form of a cylinder, 
made of the element being studied in 
tAhneo fdlaem--e-tungsten
8 
A hollow cathode lamp ffoorr AAlluummiinnuumm ((AAll))
9 
SpectrAA - AAS 
HCL 
Motorized 
Mirror
10 
Sample Atomization Techniques 
Flame Atomization 
Electro-Thermal Atomization/ 
Graphite Furnace 
Hydride Atomization 
Cold-Vapor Atomization
11
12 
Common fuels and oxidants used in flame 
spectroscopy
13 
Atomic Absorption 
Spectrophotometer
14 
Sensitivity and Detection limit (ng/mL)
15 
APPLICATIONS 
• ENVIRONMENTAL SCIENCE 
• FOOD TECHNOLOGY 
•• PHARMACEUTICALS 
• PETROCHEMICALS 
•• GEOCHEMICAL/MINING 
•• BIO-MONITORING 
•• AGRICULTURE 
• NANOMATERIALS 
• PATHOLOGY
16 
IN FORENSIC SCIENCES 
1. Determination of trace elements 
2. Elemental profiles of biological samples. 
3. Trace elements in artificial fibres. 
4. Determination of the mode of poisoning. 
5. Hair analysis for heavy metal poisons. 
6. Determinations of ammunition manufacturers. 
7. Discrimination of objects/Elements.
17
18 
Advantages and disadvantages 
High sensitivity 
[10-10g (flame), 10-14g (non-flame)] 
Good accuracy 
(Relative error 0.1 ~ 0.5 % ) 
High selectivity 
Widely used 
A resonance line source is required 
for each element to be determined
19
20

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gastroretentive drug delivery system-PPT.pptx
 

Applications of Atomic Absorption Spectrometry (AAS)

  • 1. 1 Atomic Absorption SSppeeccttrroossccooppyy (( AAAASS)) APPLICATIONS By: Tapeshwar Bhardwaj
  • 2. 2 AAttoommiicc AAbbssoorrppttiioonn SSppeeccttrroossccooppyy (( AAAASS )) · IInnttrroodduuccttiioonn · IInnssttrruummeennttaattiioonn · AApppplliiccaattiioonnss
  • 3. 3 Innttrroodduuccttiioonn What is AAS ? Atomic Absorption Spectroscopy is a quantitative method of analysis that is applicable to many metals and a few elements.
  • 4. 4 What is AAS ? Introduced in 1955 By Alan Walsh in Australia First commercial AA Spectrometer was introduced in 1955. Alan Walsh 1916-1998
  • 5. 5 What is AAS ? • An atomic absorption spectrophotometer consists of a light source, a sample compartment and a detector. SSaammppllee CCoommppaarrttmmeenntt LLiigghhtt SSoouurrccee DDeetteeccttoorr
  • 6. 6 IInnssttrruummeennttaattiioonn Line source Atomization Monochromator Detector Nebulizer Read-out Schematic diagram of a flame spectrophotomer
  • 7. 7 HHoollllooww CCaatthhooddee LLaammpp ((HHCCLL)) Cathode--- in the form of a cylinder, made of the element being studied in tAhneo fdlaem--e-tungsten
  • 8. 8 A hollow cathode lamp ffoorr AAlluummiinnuumm ((AAll))
  • 9. 9 SpectrAA - AAS HCL Motorized Mirror
  • 10. 10 Sample Atomization Techniques Flame Atomization Electro-Thermal Atomization/ Graphite Furnace Hydride Atomization Cold-Vapor Atomization
  • 11. 11
  • 12. 12 Common fuels and oxidants used in flame spectroscopy
  • 13. 13 Atomic Absorption Spectrophotometer
  • 14. 14 Sensitivity and Detection limit (ng/mL)
  • 15. 15 APPLICATIONS • ENVIRONMENTAL SCIENCE • FOOD TECHNOLOGY •• PHARMACEUTICALS • PETROCHEMICALS •• GEOCHEMICAL/MINING •• BIO-MONITORING •• AGRICULTURE • NANOMATERIALS • PATHOLOGY
  • 16. 16 IN FORENSIC SCIENCES 1. Determination of trace elements 2. Elemental profiles of biological samples. 3. Trace elements in artificial fibres. 4. Determination of the mode of poisoning. 5. Hair analysis for heavy metal poisons. 6. Determinations of ammunition manufacturers. 7. Discrimination of objects/Elements.
  • 17. 17
  • 18. 18 Advantages and disadvantages High sensitivity [10-10g (flame), 10-14g (non-flame)] Good accuracy (Relative error 0.1 ~ 0.5 % ) High selectivity Widely used A resonance line source is required for each element to be determined
  • 19. 19
  • 20. 20

Editor's Notes

  1. abbreviation