Scale up means increasing the quantity or volume of cell culture. For animal cells, the scale up strategies are dependent upon cell types or i.e. whether the cells requires matrix for attachment and growth ( adherent cell culture) or grows freely in suspended form in aqueous media. The scaling up principle for adherent cells are just to increase surface area for attachment while for suspension culture is to increase culture volume. This presentation enlightens the reader about different methods of scaling up of cells culture. Readers are also provided with sample questions for better understanding
Process scale-up is a critical activity that enables a fermentation process achieved in research and development to operate at a commercially viable scale for manufacturing.
Bioreactors for animal cell suspension cultureGrace Felciya
1. Types of culture
2. Techniques of cultivating animal cell
3. suspension culture/ Non anchorage dependent
4. Bioreactor consideration
5. Requirements of Bioreactor
6. Reactors used in cultivation
Scale up means increasing the quantity or volume of cell culture. For animal cells, the scale up strategies are dependent upon cell types or i.e. whether the cells requires matrix for attachment and growth ( adherent cell culture) or grows freely in suspended form in aqueous media. The scaling up principle for adherent cells are just to increase surface area for attachment while for suspension culture is to increase culture volume. This presentation enlightens the reader about different methods of scaling up of cells culture. Readers are also provided with sample questions for better understanding
Process scale-up is a critical activity that enables a fermentation process achieved in research and development to operate at a commercially viable scale for manufacturing.
Bioreactors for animal cell suspension cultureGrace Felciya
1. Types of culture
2. Techniques of cultivating animal cell
3. suspension culture/ Non anchorage dependent
4. Bioreactor consideration
5. Requirements of Bioreactor
6. Reactors used in cultivation
The heart of the fermentation or bioprocess technology is the Fermentor or Bioreactor. A bioreactor is basically a device in which the organisms are cultivated to form the desired products. it is a containment system designed to give right environment for optimal growth and metabolic activity of the organism.
A fermentor usually refers to the containment system for the cultivation of prokaryotic cells, while a bioreactor grows the eukaryotic cells (mammalian, insect cells, etc).
Fermentation
Scale up of fermentation
Steps in scale up
Scale up fermentation process
Optimizing scale up of fermentation process
Rules followed while doing scale up
Studies carried out during scale up
Reference
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
SYNCHRONOUS CULTURES CAN BE OBTAINED IN SEVERAL WAYS:
Physical fractionation .
Chemical appro ach
CENTRIFUGAL ELUTRIATION
Inhibition of DNA synthesis
Nutritional deprivation
SYNCHRONIZATION AT LOW TEMPERATURE
CELLULAR TOTIPOTENCY
SOME HIGHLIGHTS OF CELL SYNCHRONIZATION
REFERENCES
Steps involved in fermentation products producing a viable product output.various steps and process were explained in them. A semester syllabus of undergraduate microbiology student in his/her semester -5 in paper -6 . I think this might be helpful to you and have a good response after reading this .thank you.
The heart of the fermentation or bioprocess technology is the Fermentor or Bioreactor. A bioreactor is basically a device in which the organisms are cultivated to form the desired products. it is a containment system designed to give right environment for optimal growth and metabolic activity of the organism.
A fermentor usually refers to the containment system for the cultivation of prokaryotic cells, while a bioreactor grows the eukaryotic cells (mammalian, insect cells, etc).
Fermentation
Scale up of fermentation
Steps in scale up
Scale up fermentation process
Optimizing scale up of fermentation process
Rules followed while doing scale up
Studies carried out during scale up
Reference
INTRODUCTION
HISTORY
NEED OF SYNCHRONIZATION
SYNCHRONOUS CULTURES CAN BE OBTAINED IN SEVERAL WAYS:
Physical fractionation .
Chemical appro ach
CENTRIFUGAL ELUTRIATION
Inhibition of DNA synthesis
Nutritional deprivation
SYNCHRONIZATION AT LOW TEMPERATURE
CELLULAR TOTIPOTENCY
SOME HIGHLIGHTS OF CELL SYNCHRONIZATION
REFERENCES
Steps involved in fermentation products producing a viable product output.various steps and process were explained in them. A semester syllabus of undergraduate microbiology student in his/her semester -5 in paper -6 . I think this might be helpful to you and have a good response after reading this .thank you.
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
A bioreactor is a type of fermentation vessel that is used for the production of various chemicals and biological reactions. It is a closed container with adequate arrangement for aeration, agitation, temperature and pH control, and drain or overflow vent to remove the waste biomass of cultured microorganisms along with their products.
It gives the general knowledge about plant tissue culture. As this topic is an important aspects of plant biotechnology, it will remind a brief idea about why it is necessary.
This ppt is prepared by Sandeep Kumar Maurya , m. pharma ,department of pharmaceutical sciences, dr. harisingh gour university sagar madhya pradesh. contains fermentation technology.
Bioreactors are essential in tissue
engineering, not only because they provide an
in vitro environment mimicking in vivo conditions
for the growth of tissue substitutes, but also
because they enable systematic studies of the
responses of living tissues to various mechanical
and biochemical cues.
Plant biotechnology also known as green biotechnology is the use of biotechnology in plant or crop production. There are several techniques used such as ell culturing. Organ culture, explant culture, cell suspension culture are some culture types. This is a very useful technology in which have several applications like synthetic seed production, somaclonal variation, cybridization, hybridization.
Many important bio-products are produced by means of fermentation where microbial, plant or animal cells are employed to produce them as their metabolites.
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Live your todays life happily.Stay awaken with good practices.Maintain your physical and mental fitness by taking care of few tips.....Follow it and live your life.
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This is a powerpoint presentation solely to give a brief idea about the role of Mass Spectrometry (MS) which is one of the powerful analytical technique.This presentation describes the role of Mass Spectrometry in the field of food industry.These slides deals with the basic principle,working,components,detailed analysis etc.
Product polishing techniques in Downstream ProcessingErin Davis
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Ideal Gas Laws in chemistry for school studentsErin Davis
This is a presentation useful for understanding basic gas laws in chemistry.Moreover , this PowerPoint presentation is helpful get an overall idea about the same.
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Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
How to Make a Field invisible in Odoo 17Celine George
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3. Requirements for a bioreactor for animal cell culture
1) well-controlled environment
(Temperature , pH, DO, nutrients, and wastes)
2) supply of nutrients
3) gentle mixing
(avoid shear damage to cells)
4) gentle aeration
(add oxygen slowly to the culture medium, but avoid the
formation of large bubbles which can damage cells on
contact).
5) removal of wastes
4. o Small volume reactors
T flasks, shaker flasks ( 5 - 25 mL)
o Intermediate scale
Small, highly controlled bioreactors (1 - 5 L)
o Production scale
Large reactors (20 - 1,000 L)
Scale-up
5. A. Static flasks & roller bottle
B. Spinner flasks
C. Rotary perfusion bioreactor
D. Rotating wall bioreactor
E. Compression bioreactor
Bioreactor for Animal Cell Cultures
6.
7. Tissue flasks
Easy to use for small
scale
Roller bottles
Good control of gas phase
Labour intensive
9. Spinner flask
Used in tissue engineering
bioprocessing, in particular for cartilage
grown in static medium, even if it is still
too thin for clinical use. Mimic a
traditional stirred tank reactor
Rotating wall bioreactor
The wall of the vessel rotates, providing
an upward hydrodynamic drag force that
balances with the downward gravitational
force, resulting in the scaffold remaining
suspended in the media. As tissue grows
in the bioreactor, the rotational speed
must be increased
11. Compression Bioreactor
It provides a controllable mechanical and
physiological environment for simulating in
vivo conditions in vitro. This class of
bioreactor is generally used in cartilage
engineering and can be designed so that both
static and dynamic loading can be applied
Rotary Perfusion bioreactors
System allows a continuous
feeding of the cell chamber
from external media bottle; cells
are retained in the cell chamber
by molecular weight cut off
membrane.
12. Growth of animal cells in a suspension
Celline bioreactor
• The is a disposable, two compartment cultivation device
suitable for many cell culture applications.
• Example: The production of monoclonal
antibodies on a laboratory scale.
• Efficient cell cultivation is dependent on an optimal supply of
oxygen and nutrients.
13. • The two compartment bioreactor is designed by dividing the
bioreactor into a medium compartment and a cell
compartment.
• A semipermeable membrane between the compartments
allows small molecules to diffuse from one compartment to
the other
• Higher molecular weight molecules secreted by the
proliferating cells are retained within the cell compartment.
• The celline is perfectly suited for a wide range of applications
involving suspension cell culture, like monoclonal antibody
production or long term continuous culture maintenance.
14. Cell line in a T flask
Wheaton CELLine Bioreactors
15. Micro carrier culture
• Micro carrier cell culture is typically carried out in
spinner flasks , although other vessels such as rotating wall
microgravity bioreactors or fluidized bed bioreactors can also
support micro carrier -based cultures.
• Micro carrier culture introduces new possibilities and for the
first time makes possible the practical high yield culture of
anchorage-dependent cells.
• In micro carrier culture cells grow as monolayers on the
surface of small spheres.
• By using micro carriers in simple suspension culture
systems it is possible to achieve yields of several million
cells per millilitre.
16. Advantages of micro carrier technology
In the vaccine industry include
a. Ease of scale-up
b. Ability to precisely control cell growth conditions in
sophisticated, computer-controlled bioreactors
c. An overall reduction in the floor space and
incubator volume required for a given-sized
manufacturing operation
d. Drastic reduction in technician labour.
23. Fibers are made of a porous material
Intraluminal (Cells inside fibers )
Extra luminal (Cells outside fibers)
Permits movement of small molecules (O2 ,glucose) , but
not cells
High cell densities
Good oxygenation
Difficult to remove cells
Hollow Fiber Bioreactor
24. Plant suspension
culture
Plant Reactors
Hairy root
System bioreactor
Seed based
plant bioreactor
Seed oil body
bioreactors
Seed protein
storage vacuole
bioreactors
Chloroplast
bioreactor
25. Plant suspension cultures
In this plant cells are grown under sterile conditions as susp
ension or callus cultures and given the appropriate hormonal
supplements for growth and are used in expression of recomb
inant proteins, secondary metabolites and antibodies.
Other kinds of plant reactors:
26. Hairy root system bioreactor
• This has rhizosecretion caused
due to infection of agro-bacterium
rhizogenes and is highly stable
and suitable for different
biopharmaceuticals.
• Express recombinant proteins,
secondary metabolites and
antibodies transported to
subcellular organelles.
• Example:
expression of 80-kDa human
lysosomal protein.
27. The nuclear chromosomes of chloroplasts are inserted with
the foreign genes that are responsible for required product.
Insulin, interferons and other proteins can be prepared.
Example
High yield in the expression of human serum
albumin protein in chloroplast
Chloroplast bioreactor
28. • Seed is the most suitable bioreactor because of their large
protein accumulation during its development.
• Specificity of expression and subcellular storage environment
are the factors that will decide which seeds are used for
producing desired products.
• The advantage of these systems is that, proteins do not
degrade at ambient temperature and are stable for long
term storage.
• Example: Alpha- L- iduronidase in Arabidopsis thaliana seeds
Seed based plant bioreactors
29. There are two types of seed based plant bioreactors
Seed protein storage vacuole bioreactors
• The protein storage vacuoles in seeds contain some dominant
sub compartments like matrix, globoid and crystalloids
which are best for storing recombinant protein.
• Matrix is suitable for soluble storage proteins, globoids
for hydrolytic enzymes and crystalloids for some intrinsic
protein sequences.
30. Seed oil body bioreactors
• This bioreactor can store a large amount of
macromolecules.
• It has oleosin proteins which are ideal carriers of
heterologous proteins encircling the seed oil body.
• This also provides recognition signal for lipase binding
during oil mobilization in seedlings.
31. Vaccine antigens:
Antigens like Insulin, rotavirus enterotoxin,
anthrax lethal factor, HIV antigen, foot and mouth
disease virus antigen, heat stable toxin have been
produced in plants.
Therapeutic products:
The first successful production of a functional antibody,
namely a mouse immunoglobulin in plants, was
reported in 1989.
In 1992, C.J. Amtzen and co workers expressed
hepatitis B surface antigen in tobacco to produce
immunologically active ingredients via genetic engineering
of plants
Products
32. Nutritional components:
Carotene , Lycopene ,Flavonoid , Nutraceuticals , Fatty acidβ ,
Vitamins , Minerals , Carbohydrates .
Biodegradable plastics:
Poly hydroxy alkanoates : biodegradable polymers which
occur naturally in plants. Plant was engineered to produce
PHAs or PHBs in the various plant cell compartments.