Mechanically agitated bioreactors, pneumatically driven bioreactors, and hydrodynamically driven disposable bioreactors are the main types used in plant cell cultures. Disposable bioreactors are becoming more common as they are low cost, simple to operate, and ensure high process security by eliminating cleaning and sterilization needs. These bioreactors are primarily used for large scale production of secondary metabolites and biotransformation of compounds in the pharmaceutical industry.

1. Bio-reactors for Plant
Cell Culture
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2. BIOREACTORS
• A bioreactor is a vessel in which a chemical process is
carried out which involves organisms or biochemically
active substances derived from such organisms.
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3. Types of cell cultures
• Continuous culture
• Batch culture
• Immobilized cell lines
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4. Types of Bio-reactors Used in Plant
Cultures
• Mechanically agitated bioreactors
• Pneumatically driven bioreactors
• Hydro dynamically driven disposable bioreactors
• Non-Agitated bioreactor
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5. Mechanically agitated bioreactors
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Stirred tank reactors
Rotary Drum bioreactors

6. Pneumatically driven bioreactors
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Bubble column bioreactor
Propeller loop airlift Jet loop airlift

7. Internal and external loop airlift
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8. Disposable bioreactors
• Disposable bioreactors represent modern alternatives to such
traditional cultivation systems.These bioreactors consist of a
sterile plastic chamber that is partially filled with media(10% to
55%) , inoculated with cells and discarded after harvest.
• The single use chamber eliminating any need for cleaning or
sterilization is made of FDA-approval biocompatible plastic
such as polyethylene, polystyrene and polypropylene.
Usually, the disposable bioreactors are low cost, simple to
operate and guarantee high process security
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9. Hydro dynamically driven
disposable bioreactor
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Wave and undertow bioreactors Biowave bioreactor

10. Non agitated bioreactors
• Various types of membrane reactor were configured
namely Hollow fiber reactor,Tubular membrane
reactor, Flat plate membrane reactor with one side or two
side flow, Multimembrane reactor
• Fluidized bed reactor
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11. Uses and advantages
• These bioreactors are mostly used in suspension culture
for large scale production of secondary metabolites.
• They are used in biotransformation of compounds
• Reduces labour
• More utilization in pharmaceutical industry
• More successful biomass production than the use of plant
as bio factories
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12. Disadvantage
• Instability of the productive cell lines,
• The slowness of the cell growth and production phase
• Limited knowledge about the secondary metabolite
pathways.
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13. Thank you
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