1. AN INTERNAL PARASITE UPDATE
MEAT SHEEP ALLIANCE OF FLORIDA FALL SYMPOSIUM
OCAL A , FLORIDA - OCTOBER 14, 2017
SUSAN SCHOENIAN
Sheep & Goat Specialist
University of Maryland Extension
sschoen@umd.edu - (301) 432-2767 x343
www.wormx.info - www.sheepandgoat.com
https://www.slideshare.net/schoenian
2. PRESENTATION TOPICS
• American Consortium for Small Ruminant Parasite Control (ACSRPC)
• Parasite overview
• Anthelmintics (dewormers) 101
• Anthelmintic resistance
• Combination treatments
• Copper oxide wire particles
• Nematode trapping fungus
• Fecal egg counting
3. AMERICAN CONSORTIUM FOR SMALL
RUMINANT PARASITE CONTROL
A group of scientists, veterinarians, and extension specialists devoted to (1) developing
novel methods for sustainable control of gastro-intestinal nematodes in small ruminants
and (2) educating the stakeholders in the small ruminant industry on the most up-to-date
methods and recommendations for control of gastrointestinal nematodes.
4. WEB SITE
WORMX.INFO OR ACSRPC.ORG
Go-to place for information about internal
parasites.
Not Facebook or Google!
7. OVERVIEW
• Gastro-intestinal parasites are the
primary health problem affecting
sheep and goats worldwide.
• GI parasites can be an obstacle to
profitable and sustainable small
ruminant production in many
production systems.
• Small ruminants are more susceptible
to parasites than other farm livestock
• Goats are more susceptible than
sheep.
• Problem is worsened by drug
resistance.
10. PRIMARY ROUNDWORMS
• Haemonchus contortus
Barber pole worm
• Trichostrongylus spp.
Black scour worm
• Teladorsagia (Ostertagia)
Brown stomach worm
Eggs look the same; need to hatch larvae in order to differentiate species.
Trichostrongylus/Teladorsagia are often not differentiated even when doing larvae ID.
12. ROUNDWORMS CAN BE
HARD TO CONTROL
• Short, direct life cycles
• No intermediate host
• Ability to engage in hypobiosis
• Barber pole worm is prolific egg layer
• Lambs/kids slow to develop
immunity.
• Compromised immunity of
periparturient female.
• Widespread and growing drug
resistance
13. COCCIDIA (EIMERIA SPP.)
THE OTHER BIG PARASITE PROBLEM
• Single-cell protozoan parasite.
• More complex life cycle than roundworms.
• Host-specific
• Not all species are pathogenic (harmful).
• Affects lambs/kids mostly before and after
weaning.
• Sheep develop strong and lifetime immunity;
goats probably less so.
• Causes diarrhea, but not always, and general ill-
thrift
• Associated with poor hygiene and
management.
https://attra.ncat.org/attra-
pub/summaries/summary.php?pub=483
14. PREVENTION
• Good hygiene, nutrition, and
management.
• Coccidiostats in mineral, feed, or water
Need to feed or put in water before risk
period
1. Lasalocid (Bovatec®) - sheep
2. Monensin (Rumensin®) - goats (
horses)
3. Decoquinate (Deccox®) - both
4. Amprolium (Corid®) - ELDU, OTC
• Natural control
• Sericea lespedeza pellets
• Other (?)
• Amprolium (Corid®)- ELDU, OTC
• Sulfa drugs (Di-Methox®) - ELDU, Rx, VFD
Damages lining of small intestines.
Damage can be permanent (“runts”)
TREATMENT
COCCIDIOSIS
15. WHAT ABOUT TAPEWORMS?
MONIEZIA EXPANSA
• Tapeworms are the only parasite visible in the
feces. Diagnosed by seeing segments in
feces.
• Tapeworms tend to be non-pathogenic; not
harmful, but they’re blamed for a lot, usually
no benefit to treatment (goats?).
• Lambs develop immunity at very young age.
• Tapeworms can cause mild unthriftiness and
digestive disturbances, intestinal blockages
(rare) and affect gut motility, predisposing
lambs to enterotoxemia (occasionally).
• Treat with albendazole (Valbazen®) or
praziquantel (in some horse dewormers,
ELDU)
https://www.wormx.info/tapeworms
16. WHAT ABOUT MENINGEAL WORM?
PARALAPHOSTRONGYLUS TENIUS
• Parasite of white tail deer.
• Also called deer or brain worm.
• Sheep, goats, alpacas, and llamas are
all abnormal hosts.
• Infection requires an intermediate host,
terrestrial snail or slug.
• Causes various neurological symptoms.
• No diagnostic test in live animal.
• No FDA-approved or proven
treatment.
• Cornell University has been evaluating
treatment protocols: fenbendazole
(SafeGuard®) + anti-inflammatory drugs
(Dexamethasone or Banamine).
https://nydairyadmin.cce.cornell.
edu/uploads/doc_392.pdf
18. WHAT IS AN ANTHELMINTIC?
• Compounds used to kill gastro-intestinal
parasites without harming host.
• More commonly called wormers, dewormers or
drenches.
• Most anthelmintics have a wide margin of
safety.
• Anthelmintics have different chemistries.
• Chemistry determines which worms they are
effective against, mode of action, and
withdrawal period(s).
• Anthelmintics are grouped by chemistries.
19. THERE ARE THREE (SORT OF 4) “CHEMICAL” CLASSES
OF DEWORMERS FOR SMALL RUMINANTS (IN THE
US).
GROUP 1
Benzimidazoles
(BZ)
GROUP 2
Macrocylic lactones (ML)
GROUP 3
Nicotinic agonists
Avermectins Milbemycins Imidazo-thiazoles Tetrahydro-pyrimidines
Fenbendazole
SafeGuard®
Panacur®
Ivermectin
Ivomec®
Moxidectin
Cydectin®
Quest®
Levamisole
Prohibit®
Leva-Med®
Tramisol®
Levasol®
Morantel tartrate
Rumatel®
Albendazole
Valbazen®
Doramectin
Dectomax®
Pyrantel
Strongid®
Eprinomectin
Eprinex®
Oxfendazole
Synanthic®
20. ANTHELMINTICS FDA-APPROVED FOR SHEEP
1
Benzimidazoles
Valbazen®
sheep drench
2a
Avermectins
Ivomec®
sheep drench
2b
Milbimycins
Cydectin®
sheep drench
3
Levamisole
Prohibit®
Leva-Med®
Adult worms
Larvae (L4) Limited
Hypobiotic larvae Limited
Lungworms
Tapeworms
Liver flukes Adult stage
Coccidia
External parasites
Some
labeled for bot control
Some
Not labeled
Persistent activity
Safety
10x
pregnancy restriction
(first 30 days)
20x 5x 3x
Labeled Dosage 3 ml/100 lbs. 3 ml/26 lbs. 1 ml/11 lbs.
Depends on dilution
2 ml/50 lbs
(concentrated drench)
Meat withdrawal 7 days 11 days 7 days 3 days
21. ANTHELMINTICS FDA-APPROVED FOR GOATS
1
Benzimidazoles
3b
Morantel
Fenbendazole
SafeGuard®
Albendazole
Valbazen®
Feed premix
Rumatel
Adult worms Not approved
Larvae (L4) Not approved sporadic
Hypobiotic larvae Not approved
Lungworms Not approved
Tapeworms Not approved
Liver flukes Adult stage
Coccidia
External parasites
Persistent activity
Safety wide 10x (sheep)
pregnancy restriction
~20x (sheep)
Dosage 1.2 ml/50 lbs. 4 ml/100 lbs. Varies by product
Meat withdrawal 6 days 7 days 30 days
Milk withdrawal 0 days
22. EXTRA-LABEL ANTHELMINTICS FOR GOATS
1
Benzimidazoles
2a
Avermectins
Ivomec®
sheep drench
2b
Milbimycins
Moxidectin
Cydectin® sheep drench
3a
Levamisole
Prohibit®
Leva-Med®
Fenbendazole
SafeGuard®
Albendazole
Valbazen®
Adult worms
Larvae (L4) Limited
Hypobiotic larvae Limited
Lungworms
Tapeworms
Liver flukes Adult stage
Coccidia
External parasites Some
label for bot control
Some
Not labeled
Persistent activity
Safety wide 10x
pregnancy
restriction
20x 5x 3x
Dosage 1.1 ml/25 lbs. 2 ml/25 lbs. 6 ml/25 lbs. 4.5 ml/25 lbs. Depends on
dilution
Meat withdrawal 16 days
(1 day for each additional day used)
9 days 14 days 17 days 4 days
Milk withdrawal 4 days
(1 day for each additional day used)
7 days 9 days 8 days 3 days
24. IN WORM
• Worms develop resistance to drugs.
Can’t be killed!
Pass resistant genes onto next
generation.
• Some animals are more resistant to
parasites.
Ability to reduce parasite establishment.
Pass resistance genes onto next
generation.
IN ANIMAL
TWO KINDS OF RESISTANCE WHEN WE TALK
ABOUT INTERNAL PARASITES
25. WHAT IS ANTHELMINTIC RESISTANCE?
• Genetic ability of a worm to survive a dose
of anthelmintic which would normally be
effective.
• Only worms that survive treatment carry
genes that confer resistance.
• Result of selection through exposure of
worm population to an anthelmintic.
• When more than 5 percent of worms are
“drug tolerant”; i.e. failure to reduce FEC by
95% or more (some say 90%).
http://www.scops.org.uk/what-is-resistance.html
26. ANTHELMINTIC RESISTANCE
• In the US, worms have developed resistance to
all dewormers and dewormer classes, though it
varies by geographic region and farm.
• Worse in Southeast due to increased parasite risk
and need for deworming.
• Worse on farms that frequently deworm or use
improper deworming practices.
• Most farms have resistance to at least two
dewormers; some farms have resistance to all
dewormers/classes. 0
20
40
60
80
100
Benzimidazoles Ivermectin Levamisole Moxidectin
Maryland Virginia Georgia
29. 0
1
2
3
4
5
6
7
Susceptible Low resistance Low to moderate Moderate to high
Number of farms (n=29) resistant to
Moxidectin based on DrenchRite® tests
Maryland Virginia Georgia
31. ANTHELMINTIC RESISTANCE IS
INEVITABLE, BUT CAN BE DELAYED.
Practices that accelerate development of resistant worms
1. Frequent deworming
2. Whole flock treatments
3. Calendar based treatments
4. Treat and move strategy
5. Rotating dewormers
6. Underdosing
7. Depositing drug in mouth instead of deep into oral cavity.
8. Use of persistent activity dewormers
9. Use injectable dewormers
10. Use pour-on dewormers.
11. Use of feed dewormers*
12. Improper storage of dewormers
32. FECAL EGG COUNT
REDUCTION TEST
• Compare before and after fecal egg counts
from same animals.
• Old protocol compared post-treatment FECs
of treated animals vs. control (untreated)
group.
• ~15 animals per drug tested
• Minimum FEC of 250 epg*, preferably higher.
• Can use individual or pooled (composite)
samples.
• Cost varies. Can learn to do yourself or send
to parasitology lab.
• Labor-intensive laboratory test that
determines resistance to all dewormers
and classes from a single pooled fecal
sample (from ~10 animals).
• Minimum FEC of 500 epg.
• Also identifies larvae: % Haemonchus
Trichostrongyles eggs look the same.
• Test done exclusively by Dr. Ray Kaplan’s
lab at the University of Georgia.
• $450 per sample
DRENCHRITE® TEST
TWO WAYS TO TEST FOR
ANTHELMINTIC RESISTANCE
35. “ THERE NOW IS VERY STRONG EVIDENCE
THAT USING COMBINATION TREATMENT IS
THE BEST METHOD FOR USING
DEWORMERS AND SHOULD BE INSTITUTED
ON ALL FARMS IMMEDIATELY.”
DR. RAY KAPLAN, UNIVERSITY OF GEORGIA (JANUARY
2017)
http://www.wormx.info/combinations
36. RATIONALE FOR
COMBINATION TREATMENTS
• Most farms have resistance to at
least two of the three major groups
of dewormers; some have resistance
to all.
• At first introduction, drug efficacy is over
99%.
• Once efficacy falls below 95%, drug
resistance is present, though drug is still
useful for treatment.
• As effectiveness of dewormer decreases
(<95%), as it is used more, it provides less
and less benefit to animals.
• Below 50%, it is no longer effective as sole
There is already resistance to Zolvix® in
other countries. Zolvix® is not sold in US.
37. WHY GIVE COMBINATION
TREATMENTS?
• Contrary to popular belief, rotating between
dewormers will not prevent resistance from
developing. In fact, it will allow worms to
develop resistance to multiple drugs
simultaneously. It is no longer recommended.
• Research done in New Zealand has shown that
the best approach is to use several different
dewormers at one time as a combination
treatment.
• When combined with “best management
practices” (that help to maintain refugia),
combination treatments may improve drug
efficacy and result in a reversion back toward
susceptibility.
Most dewormers sold in New Zealand and Australia
are combination products (multiple drug actives in
same product); combination products are not
available in US.
38. HOW DO COMBINATIONS WORK?
• Unlike rotating drugs, there is an
additive effect with each drug used
in a combination treatment.
• By achieving a higher efficacy,
there are fewer resistant worms
that survive treatment.
• The sooner you start using
combination treatments the better,
as you achieve the greatest
difference in the percentage of
resistant survivors when efficacy of
dewormers is high.
Drug 1 Drug 2 Drug 3 Combo12 Combo123
80% 80% 80% 96.00% 99.20%
90% 90% 90% 99.00% 99.90%
60% 95% 98.00% 98.00%
60% 60% 95% 84.00% 99.20%
99% 99% 99.99% 99.99%
60% 60% 60% 84.00% 93.60%
50% 50% 50% 75.00% 87.50%
40% 40% 40% 64.00% 78.40%
95% 80% 20% 99.00% 99.20%
39. RECOMMENDATIONS FOR USING
COMBINATION TREATMENTS
• Purchase and administer each dewormer separately in a
separate syringe.
• Do not mix dewormers. They are not chemically
compatible. Only veterinarians have the right to
compound medications.
• Administer each dewormer at full dose based on an
accurate weight.
• Can give one drug immediately after the other.
• Observe withdrawal period of drug with longest
withdrawal period
40. RECOMMENDATIONS FOR USING DEWORMERS
IN COMBINATION
Valbazen® Cydectin® Prohibit®*
Sheep 1.5 ml/50 lbs.
[7 days]
4.5 ml/50 lbs.
[7 days]
Depends on dilution
[3 days]
Goats 4 ml/50 lbs.
[9 days meat]
[7 days milk]
9 ml/50 lbs.
[17 days meat]
[8 days milk]
Depends on dilution
[4 days meat]
[3 days milk]
44. WHAT ARE COPPER OXIDE WIRE
PARTICLES (COWPS)?
• Tiny metal rods of copper oxide
(Cu2O).
• Poorly absorbed, slow release form of
copper versus copper sulfate which is
very absorbable; therefore, greater
potential for toxicity (especially in
sheep).
• Has been shown to reduce barber
pole worm infections in sheep and
goats.
• Available as copper supplement
(different brands) for cattle (12.5 and
25 g) and goats (2 and 4 g).
https://www.wormx.info/copper-oxide-wire-particles
45. COPPER BASICS
• Sheep and goats require copper in
their diets.
• Copper metabolism is very
complicated, with several antagonists
(interactions), including molybdenum,
sulfur, zinc, cadmium, and iron.
• Copper absorption is more important
than copper concentration in diet.
• Copper absorption rates vary from
90% in young animals to 10% in mature
animals to less than 1% in the presence
of antagonists.
46. COPPER REQUIREMENTS
• Sheep: old copper requirements were 7-11 ppm
(mg/kg), or 1-8.6 ppm, depending upon
physiological state of animal [NRC, 1985]
• New requirements use factorial method (equation)
to determine copper requirements [NRC, 2007];
equation considers absorption coefficients,
antagonists (Mo, S, Fe, Cd, Zn), and interactions.
• Same data is not available for goats; requirements
are set at 15, 20, and 25 ppm for lactating,
mature, and growing goats respectively;
maximum tolerable amount is unknown.
• Goats have higher copper requirements than
sheep and are less susceptible to copper toxicity.
47. ASSESS THE COPPER
STATUS OF YOUR
FLOCK/HERD
• Sheep are highly susceptible to copper
toxicity; narrow margin between
requirement and excess; are your goats
copper deficient?
• Blood copper levels can be misleading
• Excess copper accumulates in liver.
• Collect liver and kidney samples for
testing.
• Frozen or chilled samples can be sent to
Michigan State University for testing.
https://www.animalhealth.msu.edu/
51. NEMATODE TRAPPING FUNGUS
DUDDINGTONIA FLAGRANS
• Duddingtonia flagrans is a
nematophagous fungus, meaning that it
traps, paralyzes, and consumes parasites.
• Non-chemical, biological control of the
free-living stage of nematode parasites.
• Substantially reduces number of infective
worm larvae, including multi-resistant
larvae.
• Is fed to grazing animals. Spores resist
digestion. No effect on host animal.
Passes through into manure.
• Reduces amount of reinfection from
contaminated pasture. Interrupts of life
cycle.
www.duddingtonia.co
m
52. USING FUNGUS TO CONTROL PARASITES
• Not commercially available. Not yet.
• Dr. Jim Miller from Louisiana State
University (retired) has been told that
product (fungus) should be available
sometime early 2018.
• Will need to feed every day to maintain
effect.
• Cost may be issue.
• Greatest application is probably zoo
animals.
54. QUALITATIVE
• Positive or negative. Yes or no. - or +
• Mix feces with flotation solution.
Place cover slip on meniscus. Put on
slide.
• Estimates number of eggs in a fresh sample
of manure: eggs per gram of feces (EPG).
• Mix known amount of feces (2-4 g) with
known amount of flotation solution (26-28
ml)
• Fill chambers of McMaster slide
QUANTITATIVE
FECAL EGG COUNTS (FECS)
• You can learn to do your own
fecals or send sample to a
parasitology lab.
• Microscope (100x)
Mechanical stage helpful
• McMaster egg counting slide
• Homemade flotation solution
55. THREE PRIMARY USES OF
FECAL EGG COUNTS
1. Determine treatment efficacy by comparing before and
after fecal egg counts from a group of animals (~n=15)
• Determine drug resistance on your farm
• Determine efficacy of alternative treatment(s)
2. Monitor pasture contamination
• How fast is pasture contamination building up?
• Determine when to move animals
3. Identify resistant and susceptible animals
• Differentiate between resistance and resilience
• Need sufficient parasite challenge to get data separation (at
least 500 epg group average, 1000 epg better)
By themselves,
fecal egg
counts are not
a good
diagnostic tool
for making
individual
deworming
decisions.
56. S USAN S CHOENIAN
S H E E P & G OAT S P E C I A L I S T
U N I V E R S I T Y O F M A R Y L A N D E X T E N S I O N
S S C H O E N @ U M D. E D U – ( 3 0 1 ) 4 3 2 - 2 7 6 7 X 3 4 3
S H E E PA N D G OAT. CO M – W O R M X . I N F O
Thank you for your
attention.
Questions?
Comments?
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