More Related Content Similar to Ngs technology (20) More from ShawVivian (19) Ngs technology1. Next-generation sequencing
(NGS) technologies have
progressive advantages in
terms of cost-effectiveness,
unprecedented sequencing
speed, high resolution and
accuracy in genomic analy-
ses, thus are playing an
increasing important role in
fields of oncology and immu-
nology.
The sequencing principles of
common sequencing instru-
ments on the market: (1) first,
construct a DNA template
library. Obtain DNA library
fragments (tens to hundreds
of bases in length) by
randomly breaking genomic
DNA, or construct paired-end
fragments that control the
distance distribution. Adapter
sequences were ligated to
both ends of the DNA
fragment and then denatured
to obtain a single-stranded
template library and immobi-
lized on a solid surface. (2)
Second, amplify the clones,
which performed by one of
several methods, such as
bridge PCR and emulsion
PCR. (3) Then, forming DNA
clusters or amplifying micro-
spheres of DNA cluster arrays
on a chip, performing a series
of cyclic reaction operations
using a polymerase or ligase,
and monitoring the optical
events generated in each
cycle biochemical reaction by
a microscopic detection syst-
em. Time series analysis of
the resulting array images is
performed to obtain sequenc-
es of DNA fragments. These
fragments are then assem-
bled into longer contigs
according to certain computer
algorithms.
Creative Biolabs uses the
advanced SuPrecision™ pl-
atform to support researchers
all over the world with their
sequencing needs for cancer.
NEXT GENERATION
SEQUENCING SERVICE
Creative Biolabs
Next Generation
Sequencing
Service
© 2007 - 2019 Creative-Biolabs All Rights Reserved Email: info@creative-biolabs.com Tel: 1-631-381-2994
WHAT WE DO:
Whole Genome Sequencing (WGS)
Service for Cancer
Whole Exome Sequencing (WES)
Service for Cancer
Targeted Sequencing Service for
Cancer
Whole Transcriptome Sequencing (WTS)
Service for Cancer
Immune Repertoire Sequencing (Rep-seq)
Service for Cancer
Bioinformatics Analysis Service
2. DNA Library Preparation
1 Clonal Amplification
2
Cyclic Array Sequencing
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