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Histology of Epithelium
Dr. Muhammad Ali Rabbani
Asst. Professor Anatomy
MBBS (NMC, Multan)
FCPS (Anatomy)
CHPE (Certificate in Health Professional Education)
DHPE (Diploma in Health Professional Education)
MBA (Masters of Business Administration)
MPH (Masters of Public Health)
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Learning Objectives
By the end of this session students should be able to
• Define epithelium and classify various types of epithelium
• Identify various types of simple epitheliums under
microscope and draw their histological diagrams
• Enumerate the examples of each type of simple epithelium
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Basic Types of Tissues
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Epithelium
Epithelial tissues are composed of closely aggregated polyhedral cells
adhering strongly to one another and to a thin layer of ECM, forming
cellular sheets that line the cavities of organs and cover the body
surface
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Principle Functions
• Covering, lining, and protecting
• Absorption
• Secretion
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Characteristics
• Shape & dimensions
• Shape of nucleus
• Number of layers
• Adjacent connective tissue
• Lamina propria
• Papilla
• Polarity of Epithelial Cells
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Basement Membrane
• The basal surface of all epithelia rests on a thin extracellular, felt-like
sheet of macromolecules referred to as the basement membrane
• Semi-permeable
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Basement Membrane Structure
• Basal Lamina
• Lamina Densa
• Lamina rara
• Reticular Lamina
• Structures forming Basal Lamina
• Type IV collagen
• Laminin
• Nidogen & Perlecan
• Structures forming reticular lamina
• Type III & Type VII collagen
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Basement Membrane Functions
• Filter
• Structural Support
• Attach epithelium to underlying connective tissue
• Organize integrins → maintain cell polarity
• Localize endocytosis, signal transduction etc
• Mediate cell-to-cell interactions
• Scaffold for repair
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Types of Epithlium
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Types
Epithelium
Surface
Epithelium
Simple
Stratified
Glandular
Epithelium
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a) lining the thin renal loops of Henle
b) covering the outer wall of the intestine
c) lining the inner surface of the cornea
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a) a renal collecting tubule
b) a large thyroid follicle
c) the thick mesothelium covering an ovary
Simple cuboidal epithelium.
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a) renal collecting duct
b) the oviduct lining, with both secretory and ciliated cells
c) the lining of the gall bladder
Simple columnar
epithelium
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Pseudo stratified epithelium
• Pseudostratified epithelium
appears stratified, although
some of the cells do not reach
the free surface; all rest on the
basement membrane
• A good example of
pseudostratified columnar
epithelium is that lining the
upper respiratory tract, where
the cells are also heavily ciliated.
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Glandular Epithelium
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Learning objectives
• Define glandular epithelium and explain with examples
• Differentiate the structure between serous and mucus secreting
glands
• Identify and draw histological sections of different types of glandular
epithelium with eosin and hematoxylin
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A gland is an organ of secretion made of specialized secretory cells
derived from surface epithelium on which it opens
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General Features
• Glands can be present as discrete organs or in the layers of viscera.
• The secretory cells of the glands form functional units called secretory
end pieces, which are either flask shaped (acini) or cylindrical in
shape (tubules).
• It is epithelial in origin.
• The fluid secreted by the gland contains enzymes, hormones, mucus
or fat
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• The secretory end pieces of some exocrine glands are surrounded by
star-shaped contractile cells that lie between the secretory cells and
the basement membrane.
• They are called myoepithelial cells as they share characteristics of
both epithelial and muscle cells
31
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Classification Based on the site of secretion
1. Exocrine gland: secretes its products onto a surface through ducts,
e.g. salivary glands.
2. Endocrine gland: secretes its products into the bloodstream, e.g.
thyroid glands.
3. Paracrine gland: secretes its products into the local extracellular
space affecting the surrounding cells, e.g enteroendocrine cells of
gastrointestinal tract (GIT)
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Based on the number of cells
1. Unicellular gland: composed of a single cell, e.g. goblet cells in the
respiratory and intestinal tracts.
2. Multicellular gland : composed of many cells, e.g. all glands other
than goblet cells
35
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Based on the number of ducts and shape of
secretory end piece
1. Simple gland: has one duct
2. Compound gland: has minor and major ducts
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Based on the mode of secretion
1. Merocrine gland: Secretory cells release their contents by exocytosis
(no loss of cytoplasm), e.g. most of the compound glands.
2. Apocrine gland:
Apical part of the cytoplasm of the secretory cells is lost in the process
of secretion (partial loss of cytoplasm), e.g. lactating mammary gland,
sweat gland in the axilla and external genitalia.
3. Holocrine gland: Secretory cells burst out pouring their contents,
resulting in the death of the cells (complete loss of cytoplasm), e.g.
sebaceous gland, tarsal gland
40
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Based on the nature of secretion
1. Serous gland: Parotid salivary gland
2. Mucous gland: sublingual salivary gland
3. Mixed gland (seromucous): submandibular salivary gland
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