This document describes single cell RNA sequencing services offered by Creative Biolabs. The service involves three main steps: 1) isolating single cells, 2) constructing sequencing libraries from the isolated cells, and 3) analyzing the sequencing data. Single cell RNA sequencing allows characterization of transcriptomes at the single cell level, providing insights not possible from bulk tissue analysis. Creative Biolabs has expertise in precision oncology and can provide various single cell omics solutions and data analysis services.

1. singlecell.creative-biolabs.com Email: info@creative-biolabs.com NY，
USA
SINGLE CELL RNA (SCRNA)
SEQUENCING SERVICE
Single Cell
Single Cell Isola�on
1
Tissue
Dissocia�on
Cells
Single Cell
Sequencing Service
Recent advances in various
single-cell technologies are
providing unprecedented
opportunities to discover the
differences of biological
systems at single-cell level.
Traditional average data
analyzed across bulk
populations can not
illustrate various crucial
biological phenomena.
Single cell RNA (scRNA)
sequencing service can be
divided into the following
three steps:
1) Single Cell Isolation
2) Library Construction and
Sequencing
3) Data Analysis
The scRNA-seq enables
researchers to characterize
transcriptomes at single-cell
level, which is especially
important for further under-
standing the cellular interac-
tion networks of tissues and
organs. The main advan-
tage of scRNA-seq is that
high resolution and
genome-wide range make it
possible to solve problems
that are intractable using
other methods, such as bulk
RNA-seq or single-cell
RT-qPCR.
With unrivaled expertise in
precision oncology, Creative
Biolabs has developed
integrated single cell omics
solutions for various fields,
facilitating sought-after and
innovative approaches to
discover the differences
between each single cells.
Our scientists and techni-
cians are dedicated to
advancing your single
cell-based scientific
programs from various
omics solutions to big data.
Library Construc�on and Sequencing
2
Data Analysis
3
Isola�on
Limi�ng Dilu�on
LCM
Micromanipula�on Microfluidic
FACS
Sheath
Flow
Laser
Deflector
Plates
Collection
Tubes
Multispectral
Detector
-
-
-
+
+
Charge
a
b
c
a b
Empty well
96-well MTP
Wel with 1+x cells
Cells
Cutting
Catapulting
Laser
(focused)
Laser
(defocused)
Enzyme,
Labeled Cells
Oil
10x Barcoded
Gel Beads
Single Cell
GEMs
Pipette
Cells
Isola�on Technology - High Throughput
Isolation Technology
- Low Flux
· Characterizing Heterogeneity
· Exploring Cancer Immunotherapies
· Unravelling Cancer Evolu�on
· Monitoring of Immunity
· Profiling the Tumor Microenvironment
· Virus Entry Mechanism and Therapeu�cs Discovery
· Single Cell DNA Sequencing Services
· Single Cell Methyla�on Sequencing Services
· Single Cell ATAC Sequencing Services
· Single Cell Proteomic Analysis
WHAT CAN WE DO OTHER SERVICES
scRNA Sequencing Methods
CEL-seq Drop-seq
MARS-seq
STRT-seq Tang-seq
Smart-seq
Isolation Workflow
RNA cDNA
Library Construc�on
Single Cell
RT & Second -strand
synthesis
Extraction
Sequencing Amplified cDNA
PCR
IVT
RT
Amplified RNA
Cell Subpopula�on
Ra�o Analysis
Gene Expression Profiles
Clustering Analysis
Trajectory Analysis
of Cell State
Immune Response Analysis
Regulatory Network Analysis Rare Subtype Iden�fica�on
Rare Subtype
Gene
overlap
5
25
50
75
GO
Gene Ra�o
Adjusted
p-value
10-4
10-6
10-8
10-12
10-10
10-14
Gene Set Analysis
Single Cell