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Natural killer (NK) cells are cytotoxic immune cells that play an important role in the defense against cancer. Genetic manipulation of NK cells to improve their persistence, tumor-targeting
capacity, and the ability to home to disease sites in vivo may further enhance the efficacy of NK cell-based cancer immunotherapy. At creative biolabs, we provide mRNA reprogramming as
an alternative method to genetically modify NK cells, with the potential to reprogram multiple NK cell properties that boost their antitumor function without incurring any major negative
effects on this cell population.
 Custom NK cell reprogramming by mRNA
Page 7
Workflow
mRNA Production NK Cells Expansion NK Cells Transduction Efficacy Evaluation
Cytotoxicity Assay
NK Cells Migration Assay
NK Cells Degranulation Assay
Persistence
Tumor-targeting capacity
Electroporation (Commonly used)
Lipofection
Viral vectors
Isolation of NK cells
Culture and Expansion of NK Cells
Isolation of PBMC
Analysis of purity and phenotype
mRNA design
mRNA Synthesis
Areas of Expertise
Our Capabilities
24

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NK cell reprogramming by mRNA

  • 1. Natural killer (NK) cells are cytotoxic immune cells that play an important role in the defense against cancer. Genetic manipulation of NK cells to improve their persistence, tumor-targeting capacity, and the ability to home to disease sites in vivo may further enhance the efficacy of NK cell-based cancer immunotherapy. At creative biolabs, we provide mRNA reprogramming as an alternative method to genetically modify NK cells, with the potential to reprogram multiple NK cell properties that boost their antitumor function without incurring any major negative effects on this cell population.  Custom NK cell reprogramming by mRNA Page 7 Workflow mRNA Production NK Cells Expansion NK Cells Transduction Efficacy Evaluation Cytotoxicity Assay NK Cells Migration Assay NK Cells Degranulation Assay Persistence Tumor-targeting capacity Electroporation (Commonly used) Lipofection Viral vectors Isolation of NK cells Culture and Expansion of NK Cells Isolation of PBMC Analysis of purity and phenotype mRNA design mRNA Synthesis Areas of Expertise Our Capabilities 24