Vacuoles are membrane-bound organelles found in plant and fungal cells that serve various functions. They were first observed in protozoa in 1776 and named "vacuoles" in 1841. Vacuoles have a membrane called the tonoplast that separates the vacuolar contents from the cytoplasm. There are different types of vacuoles including lytic vacuoles, protein storage vacuoles, contractile vacuoles, food vacuoles, and sap vacuoles. Vacuoles in plants and fungi maintain pH, store water and nutrients, control turgor pressure and cell growth. They also store pigments and break down materials. Animal vacuoles assist in exocytosis and

1. VACUOLES
Presented By:
Inchara R
6th Semester
Molecular Biology
28/05/2020
Guided By:
Dr. Jagadeesh D
Dept. of Molecular Biology

2. CONTENTS
 Introduction
 Discovery
 Structure
 Types of vacuoles
 Functions
 Conclusion
 References
 Acknowledgement

3. INTRODUCTION
Fig 01: Plant Vacuole

4. DISCOVERY
 Contractile vacuoles were first observed by Spallanzani
(1776) in protozoa, although mistaken for respiratory
organs.
 Dujardin (1841) named these "stars" as vacuoles.
 In 1842, Schleiden applied the term for plant cells, to
distinguish the structure with cell sap from the rest of
the protoplasm.
 In 1885, de Vries named the vacuole membrane as
tonoplast.

5. STRUCTURE
Fig 02: Structure of Central Vacuole

6.  They generally have no basic shape or size; its structure varies
according to the requirements of the cell.
 In immature and actively dividing plant cells the vacuoles are quite
small. These vacuoles arise initially in young dividing cells,
probably by the progressive fusion of vesicles derived from the
Golgi apparatus.
 A vacuole is surrounded by a membrane called the tonoplast or
vacuolar membrane and filled with cell sap.
 The tonoplast is the cytoplasmic membrane surrounding a vacuole,
separating the vacuolar contents from the cell’s cytoplasm. As a
membrane, it is mainly involved in regulating the movements of
ions around the cell, and isolating materials that might be harmful
or a threat to the cell

7. TYPES OF VACUOLES
 Lytic vacuoles: These are plant specialized vacuoles, function as
reservoirs for ions and metabolites, including pigments, and are crucial to
processes of detoxification and general cell homeostasis.
 Protein storage vacuoles (PSV): Storage proteins are deposited into protein
storage vacuoles (PSVs) during plant seed development and maturation
and stably accumulate to high levels; subsequently, during germination
the storage proteins are rapidly degraded to provide nutrients for use by
the embryo.
 Contractile vacuoles: Contractile vacuoles are membrane bound organelles
that are typically found among members of kingdom Protista (algae,
amoeba, and ciliates). In these cells, the contractile vacuole is particularly
important given that it helps in osmoregulation (regulation of osmotic
pressure).

8.  Food Vacuoles: A food vacuole is formed by the fusion of a phagosome
and a lysosome. The food vacuole contains digestive enzymes with the
help of which nutrients are digested. The digested materials pass out
into the surrounding cytoplasm.
 Sap vacuoles: It is the large, central organelles that occupy most part
of the cell volume. This organelle contains the fluid known as the cell
sap or vacuolar sap, which consists of such contents as water, sugars,
minerals and amino acids among others.
 Gas vacuoles: Gas vacuoles are composed of hollow cylindrical gas
vesicles. They are typically found in bacteria and have a permeable
membrane that allows air to pass through. Air vacuoles not only store
gases but provide buoyancy, mechanical strength and protection from
harmful radiations.

9. FUNCTIONS
 In plants & fungi:
 Vacuoles in plant and fungi cells perform more functions than
vacuoles in other types of cells. Because fungi and plant cells don’t
contain lysosomes, vacuoles in these cells also break down more
materials than they do in animal cells.
 Maintain proper pH.
 Store water.
 Maintain turgor pressure.
 Cell growth.
 Adjust size of the cell.

10.  In Animals:
 Vacuoles perform mostly subordinate roles, assisting in larger
processes of exocytosis and endocytosis. Animal vacuoles are
smaller than their plant counterparts but also usually greater in
number. There are also animal cells that do not have any vacuoles.
 Some of the other functions include:
 Storage - Apart from proteins, vacuoles are storage compartments
for metabolites, organic acids and sugars among others
 Pigment deposition - Vacuoles are a site where pigments are
deposited allowing for such vegetable colors as red, blue, scarlet
etc.

11. CONCLUSION

12. REFERENCES
 Verma P. S. and Agarwal V. K. 2006. Cell Biology, Genetics, Molecular
Biology, Evolution & Ecology, S. Chand and company Ltd., 1291pp.
 https://microbenotes.com/vacuoles-structure-types-and-functions/
 https://www.sciencedirect.com/science/article/abs/pii/S1369527408
001318
 https://biologydictionary.net/vacuole/

13. ACKNOWLEDGEMENT
I would like to thank the dept. of Molecular Biology for
providing this opportunity to present this seminar.
I would also like to thank my guide Dr. Jagadeesh for his
valuable guidance.
Thank you one and all.