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Plant cell – Nucleus, Chloroplast, Mitochondria,
Golgi apparatus, Cell wall, Plasma membrane, ER,
Peroxisomes and Glyoxysomes – Structure and
functions.
Cell
Latin word Cella = “small room”
1. It is the basic structural, functional, and biological
unit of all known living organisms
2. Often called the "building blocks of life".
3. The study of cells is called cell biology.
4. The cell was discovered by Robert Hooke in 1665.
Cell
Cell Wall
{Non living}
Protoplasm
{Living}
Cytoplasm Nucleus
Cytosol Cell organelles
Primary Wall
Middle Lamella
Secondary Wall
Found in plant cells
Non-living component
Secreted and maintained by protoplasm
A typical cell wall is composed of three
different regions
1. Middle Lamella
2. Primary cell wall (1-3 μm thick and elastic)
3. Secondary cell wall (5-10 μm thick and rigid)
Functions
Protects the inner contents of the cell
Gives definite shape to the cell
It provides mechanical support to the tissues
Transport of substances between two cells
Hydrophilic in nature
Permeable structure - absorption of minerals
and solutes
CELL WALL
Protoplasm
Cell devoid of cell wall is
called protoplast.
Living, colloidal and semi
fluid substance
Also called as cytoplasm.
Protoplast is enclosed by
a membrane called as cell
membrane or plasma
membrane. Protoplast of Petunia leaf
All cells are enclosed by a thin,
membrane called plasma membrane
or plasma lemma.
Cell membrane consists of proteins,
lipids and other substances
Found both in plant and animal cells
Functions
Surrounds the protoplasm of the cell
Anchors the cytoskeleton
Differentially permeable- regulate
the transport across the membrane.
Maintain the cell potential
CELL MEMBRANE
Oval or spherical in shape
Larger in active cells than in resting cells
Three main parts
1. Nuclear envelope
* Separated from the cytoplasm by a double
membrane called the nuclear envelope
* Space between the outer and inner
membrane is known as nuclear pores
2. Nucleolus
* Spherical, colloidal body found in the
nucleus
* DNA replication and RNA synthesis occur
3. Chromatin
* Basic unit of chromosome
* Contains genes which play important role
in the inheritance of characters to
offspring from parents.
CELL NUCLEUS
Functions
Regulates growth and
reproduction of cells
Nuclear envelope allows the
nucleus to control its contents
- separate them from the rest
of the cytoplasm
DNA replication,
transcription and post
transcriptional modification
occur in the nucleus.
CELL NUCLEUS
Found in plant cells and other eukaryotic
organisms that perform photosynthesis
Green pigment, chlorophyll present.
Surrounded by double layered membrane.
Space between these two layers is called
intermembrane space.
Stroma - Aqueous fluid found inside the
chloroplast.
It contains the machinery required for
carbon fixation, circular DNA, 70 S
ribosomes (thats why called as
semiautonomous organelle) etc.
Within the stroma the stacks of thylakoids
are arranged as stacks called grana.
A thylakoid has a flattened disc shape and
has a lumen or thylakoid space.
Light reactions – thylakoid membrane.
Functions:
Photosynthesis i.e, light and
dark reactions occur
The granum – site of light
reaction of photosynthesis
The stroma - main site for
the dark reaction of
photosynthesis.
Chloroplast has its own
genetic system and is self
replicating. Thus, associated
with cytoplasmic inheritance.
MITOCHONDRIA
*Rod shaped cytoplasmic organelles
*Main sites of cellular respiration - power house of the cell
*Space between the two membranes is called perimitochondrial space
*Inner membrane has a series of infoldings known as cristae
*Inner space enclosed by cristae - filled by a relatively dense material
known as matrix.
Matrix contains
several copies of
round or circular
DNA molecules and
70 S ribosomes
Functions
ATP (readily available form of
energy) - produced in
mitochondria.
Krebs cycle - matrix of
mitochondria.
The enzymes of electron
transport chain -found in the
inner membrane.
Heme synthesis occurs in
mitochondria.
Controls the cytoplasmic Ca2+
concentration
 Ribosomes are ribonucloprotein complexes
 Ribosomes - Prokaryotes – 70S= 50S & 30S
Eukaryotes – 80S = 60S & 40S
 Two or more ribosomes - connected by a single
m RNA and then may be called polyribosome
 Smaller subunit of ribosome provide proper
site for binding of mRNA and its translation.
 Larger subunit of ribosome supports
translation and translocation processes
coupled with polypeptide synthesis.
Functions:
1. They provide the platform for protein
synthesis
2. They have the machinery for protein synthesis.
Dictyosomes or Golgi Apparatus or
Golgi Complex
Long flattened vesicles with bulged
end – Cisternae
Mostly linked with ER
Functions :
1. It helps in Packaging of proteins
for exporting them.
2. It plays a role in sorting of
proteins for incorporation into
organelles.
3. It is involved in the formation of
the cell wall of plant cells
Endoplasmic reticulum
*Arises from the outer membrane of the
nucleus
It is of two types.
1. Granular or rough endoplasmic
reticulum in which the outer surface of
endoplasmic reticulum is studded with
ribosome
2. Agranular or smooth endoplasmic
reticulum in which the ribosomes are not
attached.
Functions :
1. RER - Synthesis of proteins.
2. SER - Synthesis of lipids and glycogen.
3. Acts as an inter-cellular transport system
for various substances.
4. It contains many enzymes which perform
various synthetic and metabolic activities.
Membrane bound organelle found in plant cell
Surrounded by a single membrane - tonoplast.
It is an enclosed compartment
Functions :
1. Stores waste products.
2. Maintains internal hydrostatic pressure or
turgor and acidic internal pH within the cell.
3. Exports unwanted substances from the cell.
4. Structural support – Gives rigidity
5. Most plants stores chemicals in the vacuole that
react with chemicals in the cytosol.
6. In seeds, stored proteins needed for
germination are kept in protein bodies which
are modified vacuole.
7. Contains pigments - Anthocyanin
Difference between vacuole of animal and plant cell
• Ubiquitous organelles - eukaryotic plant
cells.
• Mostly spherical with diameter 0.2um -
1.5um. Two types of microbodies
1. Peroxisomes
• Surrounded by single layer membrane
• Contains key enzymes for glycolate cycle
• Peroxisomal enzyme – Catalase
• Site of photorespiration
2. Glyoxysomes
• Found in fat rich seeds (Oil storing seeds)
• Contains key enzymes for Glyoxylate cycle
• Conversion of fatty acids in to sugars
 Present within the cytoplasm
 Made up of protein
 Present in all cells
 Three main kinds of cytoskeleton filaments:
1. Microfilament: Composed of actin subunits.
2. Intermediary filaments: Maintain cell shape
by bearing tension.
3. Microtubules: Hollow cylinders mostly
comprising of 13 protofilaments which in
turn are alpha and beta tubulin.
Functions:
1. Provides mechanical support
2. Anchors organelles
3. Helps to move substances intracellular.
♦ Plasmodesmata (singular: plasmodesma)
♦ Microscopic channels - traverse the cell walls of
plant cells and some algal cells
♦Enabling transport and communication between
them.
♦ Specialized cell-to-cell communication pathways
known as Plasmodesmata, pores in the primary cell
wall through which the plasmalemma and
endoplasmic reticulum of adjacent cells are
continuous.
♦ Middle lamella forms an extracellular domain –
apoplast.
♦ Plasmodesmata enable direct, regulated, symplastic
transport of substances between cells.
Two forms of plasmodesmata:
♦ Primary - formed during cell division
♦ Secondary - form between mature cells.
Cphy 161 lec-2 (plant cell)

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Cphy 161 lec-2 (plant cell)

  • 1. Plant cell – Nucleus, Chloroplast, Mitochondria, Golgi apparatus, Cell wall, Plasma membrane, ER, Peroxisomes and Glyoxysomes – Structure and functions.
  • 2.
  • 3. Cell Latin word Cella = “small room” 1. It is the basic structural, functional, and biological unit of all known living organisms 2. Often called the "building blocks of life". 3. The study of cells is called cell biology. 4. The cell was discovered by Robert Hooke in 1665.
  • 5.
  • 6. Primary Wall Middle Lamella Secondary Wall Found in plant cells Non-living component Secreted and maintained by protoplasm A typical cell wall is composed of three different regions 1. Middle Lamella 2. Primary cell wall (1-3 μm thick and elastic) 3. Secondary cell wall (5-10 μm thick and rigid) Functions Protects the inner contents of the cell Gives definite shape to the cell It provides mechanical support to the tissues Transport of substances between two cells Hydrophilic in nature Permeable structure - absorption of minerals and solutes CELL WALL
  • 7. Protoplasm Cell devoid of cell wall is called protoplast. Living, colloidal and semi fluid substance Also called as cytoplasm. Protoplast is enclosed by a membrane called as cell membrane or plasma membrane. Protoplast of Petunia leaf
  • 8. All cells are enclosed by a thin, membrane called plasma membrane or plasma lemma. Cell membrane consists of proteins, lipids and other substances Found both in plant and animal cells Functions Surrounds the protoplasm of the cell Anchors the cytoskeleton Differentially permeable- regulate the transport across the membrane. Maintain the cell potential CELL MEMBRANE
  • 9. Oval or spherical in shape Larger in active cells than in resting cells Three main parts 1. Nuclear envelope * Separated from the cytoplasm by a double membrane called the nuclear envelope * Space between the outer and inner membrane is known as nuclear pores 2. Nucleolus * Spherical, colloidal body found in the nucleus * DNA replication and RNA synthesis occur 3. Chromatin * Basic unit of chromosome * Contains genes which play important role in the inheritance of characters to offspring from parents. CELL NUCLEUS
  • 10. Functions Regulates growth and reproduction of cells Nuclear envelope allows the nucleus to control its contents - separate them from the rest of the cytoplasm DNA replication, transcription and post transcriptional modification occur in the nucleus. CELL NUCLEUS
  • 11. Found in plant cells and other eukaryotic organisms that perform photosynthesis Green pigment, chlorophyll present. Surrounded by double layered membrane. Space between these two layers is called intermembrane space. Stroma - Aqueous fluid found inside the chloroplast. It contains the machinery required for carbon fixation, circular DNA, 70 S ribosomes (thats why called as semiautonomous organelle) etc. Within the stroma the stacks of thylakoids are arranged as stacks called grana. A thylakoid has a flattened disc shape and has a lumen or thylakoid space. Light reactions – thylakoid membrane.
  • 12. Functions: Photosynthesis i.e, light and dark reactions occur The granum – site of light reaction of photosynthesis The stroma - main site for the dark reaction of photosynthesis. Chloroplast has its own genetic system and is self replicating. Thus, associated with cytoplasmic inheritance.
  • 13. MITOCHONDRIA *Rod shaped cytoplasmic organelles *Main sites of cellular respiration - power house of the cell *Space between the two membranes is called perimitochondrial space *Inner membrane has a series of infoldings known as cristae *Inner space enclosed by cristae - filled by a relatively dense material known as matrix. Matrix contains several copies of round or circular DNA molecules and 70 S ribosomes
  • 14. Functions ATP (readily available form of energy) - produced in mitochondria. Krebs cycle - matrix of mitochondria. The enzymes of electron transport chain -found in the inner membrane. Heme synthesis occurs in mitochondria. Controls the cytoplasmic Ca2+ concentration
  • 15.  Ribosomes are ribonucloprotein complexes  Ribosomes - Prokaryotes – 70S= 50S & 30S Eukaryotes – 80S = 60S & 40S  Two or more ribosomes - connected by a single m RNA and then may be called polyribosome  Smaller subunit of ribosome provide proper site for binding of mRNA and its translation.  Larger subunit of ribosome supports translation and translocation processes coupled with polypeptide synthesis. Functions: 1. They provide the platform for protein synthesis 2. They have the machinery for protein synthesis.
  • 16. Dictyosomes or Golgi Apparatus or Golgi Complex Long flattened vesicles with bulged end – Cisternae Mostly linked with ER Functions : 1. It helps in Packaging of proteins for exporting them. 2. It plays a role in sorting of proteins for incorporation into organelles. 3. It is involved in the formation of the cell wall of plant cells
  • 17. Endoplasmic reticulum *Arises from the outer membrane of the nucleus It is of two types. 1. Granular or rough endoplasmic reticulum in which the outer surface of endoplasmic reticulum is studded with ribosome 2. Agranular or smooth endoplasmic reticulum in which the ribosomes are not attached. Functions : 1. RER - Synthesis of proteins. 2. SER - Synthesis of lipids and glycogen. 3. Acts as an inter-cellular transport system for various substances. 4. It contains many enzymes which perform various synthetic and metabolic activities.
  • 18. Membrane bound organelle found in plant cell Surrounded by a single membrane - tonoplast. It is an enclosed compartment Functions : 1. Stores waste products. 2. Maintains internal hydrostatic pressure or turgor and acidic internal pH within the cell. 3. Exports unwanted substances from the cell. 4. Structural support – Gives rigidity 5. Most plants stores chemicals in the vacuole that react with chemicals in the cytosol. 6. In seeds, stored proteins needed for germination are kept in protein bodies which are modified vacuole. 7. Contains pigments - Anthocyanin
  • 19. Difference between vacuole of animal and plant cell
  • 20. • Ubiquitous organelles - eukaryotic plant cells. • Mostly spherical with diameter 0.2um - 1.5um. Two types of microbodies 1. Peroxisomes • Surrounded by single layer membrane • Contains key enzymes for glycolate cycle • Peroxisomal enzyme – Catalase • Site of photorespiration 2. Glyoxysomes • Found in fat rich seeds (Oil storing seeds) • Contains key enzymes for Glyoxylate cycle • Conversion of fatty acids in to sugars
  • 21.  Present within the cytoplasm  Made up of protein  Present in all cells  Three main kinds of cytoskeleton filaments: 1. Microfilament: Composed of actin subunits. 2. Intermediary filaments: Maintain cell shape by bearing tension. 3. Microtubules: Hollow cylinders mostly comprising of 13 protofilaments which in turn are alpha and beta tubulin. Functions: 1. Provides mechanical support 2. Anchors organelles 3. Helps to move substances intracellular.
  • 22. ♦ Plasmodesmata (singular: plasmodesma) ♦ Microscopic channels - traverse the cell walls of plant cells and some algal cells ♦Enabling transport and communication between them. ♦ Specialized cell-to-cell communication pathways known as Plasmodesmata, pores in the primary cell wall through which the plasmalemma and endoplasmic reticulum of adjacent cells are continuous. ♦ Middle lamella forms an extracellular domain – apoplast. ♦ Plasmodesmata enable direct, regulated, symplastic transport of substances between cells. Two forms of plasmodesmata: ♦ Primary - formed during cell division ♦ Secondary - form between mature cells.