3. OUT LINES OF PRESENTATION
Introduction 11
Benefits of tissue
engineering
22
Aims And Objects 33
Procedure 44
Potential of stem
cells in tissue
engineering
55
Stem cells applications 66
Features of tissue
engineering
77
5. Its ultimate goal is to be a cure, not merely treatment – by repairing or replacing tissues and
organs that fail due to disease, genetic errors, congenital abnormalities
TISSUEENGINEERINGIN
LABORATORY
7. For example Tissue engineering is the field of biomedical
engineering that’s goal is to create new cells
that helps heal organs and if need be, to
create an entirely new organ.
9. Benefits of tissue
engineering
The mainpoint of tissueengineeringis to
makeit easierbasicallyreproduce organs
in order to makepeople’s lives better.
10. AIMS AND OBJECTS
Tissue engineering is multidisciplinary by necessity
“an interdisciplinary field that applies the principles of engineering and life sciences
towards the development of biological substitutes that restore, maintain, or improve
tissue function or a whole organ”
12. Procedure
There are five basic steps in tissue engineering.
1st step is actually
getting the base cells
to work with, from
patient.
2nd Expending cells3rd Seed onto an
appropriate scaffold with
suitable growth factors
and cytokines.
4th step is putting the altered
cells into a scaffold in order
to incubate the cell
5th The final step is to put the
newly created cells or organ
into use
13. Step 01
These cells are then
modified to whatever kind
of cell is needed.
There are 7 sources from
where you can get these
cells.
The materials that are used for tissue engineering are
actually living cells that were taken from somewhere else.
1. Autologous-.
2. Allogenic-
3. Xenogenic-
4. Syngenic-
5. Primary-
6. Secondary-
7. Stemcells-
18. 5. Primary- Come from any organism.
6.Secondary- Come from a cell bank.
7. Stem cells- Undifferentiated cells.
19. Bioreactors are used
in an attempt to
recreate realistic
physiological
environments so that
the cells can grow in
a natural manner.
Step 02
Expending cells in laboratory
20. Step 03 and 04
Once the cells are obtained, they are then put
into a scaffold to let the cells grow into their
new forms.
Scaffolds are materials that have been
engineered to cause desirable cellular
interactions to contribute to the formation of
new functional tissues for medical purposes.
Cells are often 'seeded' into these structures
capable of supporting three-dimensional tissue
formation.
Capillary Culture Units:
The original problem with creating new organs
was that they would die before they could get
placed into the organism that needed them.
This problem has been solved with the recent
developments in 3D printers, which allows for
blood vessels to be put into the new organ.
that delivers nutrients and removes products
of cellular metabolism.
The capillary culture unit (CCU)
consists of a network of artificial
capillaries that simulates the in vivo
vascular matrix.
21. Step 05
This step obviously is when the newly created cells are put into use.
Whether they are being used to create a new organ via the 3D Organ
Printer, or they are being used as new skin.
22. POTENTIAL OF STEM CELLS IN TISSUE ENGINEERING
Capable of self-renewal-- can divide and renew themselves for long periods
Unspecialized cells that can differentiate into other types of cells
23. Pluripotent: Embryonic stem cells:
Can become any cell type in the body
Enormous potential
Self-renewal
Multipotent: Adult stem cells.
Can become multiple but limited number
of cell types.
Derived From Patient
Reduced Risk Of Immune Rejection
24. It is renewable source of
replacement cells and tissues to
replace need for donors.
potential to treat diseases or
injuries that affect tissues that
cannot regenerate
CURRENT RESEARCH APPLICATIONS:
Cardiovascular disease:
Diabetes,
Osteoarthritis,
Spinal cord injury,
Alzheimer’s,
Strokes,
Burns,
Drug discovery,...
STEM CELL APPLICATIONS:
32. FEATURESOF TISSUEENGINEERING
Artificial skin constructed from human skin cells embedded in a hydrogel, such as in the
case of bioprinted constructs for battlefield burn repairs
35. I see tissue engineering as a very promising field in Biomedical engineering. It provide
us a wide view of advantages of advanced bioengineering.
It can solve many of the problems that people experience today. Like:-
This field has developed great hope for the peoples who suffer of liver ailments by
generating artificial liver.
It has reawaked hope in the diabetic patients by producing artificial pancreatic Beta
cells of Iselets of Langhans for insuline.
Tissue engineering is a exciting, interesting and valuable field where is Pakistan lot of
work is needed specially in research and development. No doubt developed countries
are more advanced in this field, and I am confident in future it would be most great
business of world, to sell and purchase human synthetic organs, people would never
talk to purchase Japanese original cars, Taiwan original mobile or other non living
devices but they would talk I have new original kidney from xy lab or country, I have to
buy a new liver ……..
DISCUSSION
36. As a researcher our up coming decade would give us more challenges, by giving exiting
research topics in tissue engineering. Emphasis is still placed on the optimal cell
sources, growth factors, scaffold design and fabrication , and the development of
microfibrication technology to create vascularized tissues and organs.
DISCUSSION
Thanks
