This document discusses substrate level phosphorylation in glycolysis. Glycolysis is the process by which glucose is broken down into pyruvate, producing a net yield of 2 ATP molecules per glucose through substrate level phosphorylation at two steps. In step 7, 1,3-bisphosphoglycerate is converted to 3-phosphoglycerate with the generation of ATP. In step 10, phosphoenolpyruvate is converted to pyruvate with another ATP generated. These substrate level phosphorylation steps contribute significantly to ATP production in glycolysis, especially under anaerobic conditions.

1. TOPIC:- SUBSTRATE LEVEL PHOSPHORYLATION IN GLYCOLYSIS
GUIDED BY:-
TRIDIP BORUAH
PG Department Of Botany
M.C. COLLEGE, BARPETA
PRESENTED BY:-
NAME:- SUSUMA PAUL
CLASS:- M.Sc. 2nd Semester

2. CONTENT
 INTRODUCTION
 GLYCOLYTIC PATHWAY AND SUBSTRATE LEVEL PHOSPHORYLATION
 CONCLUSION

3. INTRODUCTION
 Glycolysis ( Glycos = glucose and lysis = breakdown) is a process where one
glucose molecule converted into 2 molecule of pyruvate.
 This process takes place in the cytosol. Hence it is also called cellular respiration.
 It is also known as Embden-Meyerhof-Parnas (EMP) pathway.
There are two phases in glycolysis-
I. Preparatory phase
II. Payoff phase

4. Fig. Glycolytic Pathway
Preparatory phase
Payoff phase

5. Reaction No. Consuption/ gain of ATP Gain of NADH
1 Consumption 1 ATP
3 Consumption 1 ATP
6 Gain 2 NADH
7 Gain 2 ATP
10 Gain 2 ATP
Net gain of ATP = 4-2=2 and
NADH=2
NET ENERGY YEILD IN GLYCOLYSIS:-

6. Substrate level phosphorylation in glycolysis
Step-7
1,3-bisphosphoglycerate 3-Phosphoglycerate
ADP ATP
Step- 10
Phosphoenolpyruvate Pyruvate
ADP ATP
Phosphoglycerate kinase
Pyruvate kinase

7. CONCLUSION
Substrate level phosphorylation in glycolysis is a vital process that contribute to ATP
production,crucial for cellular energy metabolism,particularly in anaerobic condition.
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8. References
 Studies in botany II
https://images.app.goo.gl/cyjMMW6R1TFab85m6
 https://images.app.goo.gl./JzMNzM9uTQDwM8ZY8
 https://www.slideshare.net/PrakashPokhrel1/glycolysis-61531085

9. THANK YOU