The pyruvate dehydrogenase complex (PDH) catalyzes the irreversible oxidative decarboxylation of pyruvate to form acetyl-CoA. PDH is an assembly of three individual enzymes - pyruvate dehydrogenase (E1), dihydrolipoyl transacetylase (E2), and dihydrolipoyl dehydrogenase (E3) - that work together to decarboxylate and oxidize pyruvate, transfer the acetyl group to CoA, and reoxidize dihydrolipoamide. PDH plays an important role in linking glycolysis to the citric acid cycle by converting pyruvate to acetyl-CoA in cells containing mitochondria.

1. PYRUVATE
DEHYDROGENASE
COMPLEX
SUPERVISED BY
MR. TRIDIP BORUAH
ASSISTANT PROFESSOR
M.C. COLLEGE, BARPETA
Submitted by
Monika Das
M.Sc 2nd semester
(Botany)
MC College, Barpeta

2. PRUVATE DEHYDROGENASE COMPLEX
(PYRUVATE OXIDATION)
• THE PYRUVATE DEHYDROGENASE COMPLEX CATALYZES THE IRREVERSIBLE
OXIDATIVE DECARBOXYLATION OF PYRUVATE TO FORM ACETYL-COA. THIS
REACTION LINKS GLYCOLYSIS AND THE CITRIC ACID CYCLE IN EVERY CELL
CONTAINING MITOCHONDRIA.

3. CONVERSION OF PYRUVATE TO ACETLY COA

4. PDH COMPLEX
• Pyruvate Dehydrogenase complex is an
assembly of three individual enzymes:
Pyruvate Dehydrogenase
(E1),Dihydrolipoyl transacetylases (E2)
and Dihydrolipoyl dehydrogenase (E3).

5. TPP : Thiamine phyrophosphate
L : Lipoyl group
CoA : Co enzyme A
PYRUVATE DEHYDROGENASE
COMPLEX
Decarboxylation
and oxidation of
pyruvate
Catalyses transfer of acetyl
Group to CoA
Reoxidizes
dihydrolipoamide

6. REGULATION BY END PRODUCT INHIBITION

7. CONCLUSION
• IN PYRUVATE DEHYDROGENASE COMPLEX THERE ARE THREE MULTIENZYME
COMPLEX PYRUVATE DEHYDROGENASE COMPLEX (E1), DIHYDROLIPOYL
TRANSACETYLASE(E2) AND DIHYDROLIPOYL DEHYDROGENASE (E3) WHICH
INCREASES THE EFFICIENCY OF THE REACTION. ALSO FIVE COENZYMES ARE
INVOLVED THIAMINE PHYROPHOSPHATE (TPP), LIPOIC ACID, FAD, NAD+ AND
CO ENZYME A ACTING ASSOCIATION WITH E1,E2 AND E3 IN PDH COMPLEX.
ACETLY –COA AND NADH , BOTH ARE THE END PRODUCT OF PDH COMPLEX.
AGAIN , 2.5 ATP IS FORMED DURING THE REACTION.

8. REFERENCE
• LIFE SCIENCES BY PATHFINDER (VOL 1)
• FUNDAMENTALS OF BIOCHEMISTRY BY DR. J.L. JAIN, DR. S. JAIN AND NITIN JAIN

9. THANK YOU