The document discusses different types of absorption and emission spectroscopy techniques. It describes UV spectroscopy, IR spectroscopy, atomic absorption spectroscopy, fluorimetry, and flame photometry. For each technique it covers the light source, filters, monochromator, detectors, and applications. Overall the document provides an overview of various spectroscopy methods, the core components used, and their uses in analyzing samples.

1. SPECTROSCOPY
Different types of Absorption and Emission spectroscopy
Rahul Sharma

2. Types of Spectroscopy
Absorption
Spectroscopy
Emission
Spectroscopy
★ UV Spectroscopy
★ IR Spectroscopy
★ Atomic Absorption Spectroscopy
★ Fluorimetry
★ Flame Photometry

3. UV IR Fluorimetry
In UV we measure absorption as a
function of wavelength in nm.
Source of light-:
I.Tungsten lamp
II.Deuterium lamp
III.Mercury vapour lamp
Filters-:
I.Absorption filter
II.Interference filter
Monochromator:-
I.Prism
II. Grating
Sample cell material:-
•Quartz (crystalline silica)
In IR we measure % transmittance as
a function of wavenumber in cm–1
.
Source of light-:
I.Tungsten halogen lamp
II.Nernest glower
III.Incandescent lamp
IV.Globar source
V.Mercury Lamp
Filters-:
-----------
Monochromator:-
I.Prism
II.Grating
Sample cell material:-
•Nacl or Kbr
In this we measure photons that are
emitted from excited sample.
Source of light-:
I.Mercury vapour lamp
II.Xenon lamp
III.Tungsten lamp
Filters-:
I.Primary filter
II.Secondary filter
Monochromator:-
I.Excitation monochromator
II.Emission monochromator
Sample cell material:-
• Quartz

4. UV IR FLUORIMETRY
Detectors:-
I.Photo Voltaic Cell
II.Photo tube /Photoemissive cell
III.Photo Multiplier Tube (PMT)
Application:-
I.Detection of impurities.
II. Structure elucidation of organic
compound.
III. Estimation of amount of drug.
IV. Detection of functional groups.
V. Molecular weight determination.
VI. As HPLC detector.
VII. Chemical kinetics.
Detectors:-
I.Thermal Detector
II.Photon Detector
Application:-
I. Identification of functional groups.
II. Identification of H-bonding.
III. Identification of cis-trans isomers.
IV. Identification of polymers,resins.
V. Detection of impurities and
additives.
VI. Studying the progress of
reaction.
Detectors:-
I.Photo Voltaic cells
II.Photo Multiplier Tube (PMT)
Application:-
I. Analysis of medicinal compound.
II. Detection of impurities.
III. Used as flourescent indicator.
IV. Determination of Vit. B1 and B2.
V. Organic analysis.

5. Flame Photometry Atomic absorption
It is a the measurement of intensity of light emitted at a
particular wavelength from the atoms that a excited
thermally in a plane.
Source of light:- Burner with fuel and oxidant.
Types of burner-:
I.Total consumption burner.
II.Premix/Laminar-Flow burner.
Filters
Monochromator. Same as UV
Detectors
It is a very common technique for detecting
metals and metaloids in a sample.
Source of light:-
❏ Halogen Cathode Lamp(HCL).
❏ Burner with fuel and oxidant.
Filters
Monochromator Same as UV
Detectors