SlideShare a Scribd company logo
SOS IN ENVIRONMENTAL CHEMISTRY
JIWAJI UNIVERSITY
GWALIOR
PRESENTATION ON – ATOMIC ABSORPTION
SPECTROSCOPY
Presented to Presented by
Dr . NIMISHA JADON AKASH SAXENA
(Assistant Professor) M.Sc. ICA (2nd
SEM)
CONTENTS
Introduction
History
Principle
Instrumentation
Interferences
Advantages
Disadvantages
Applications
References
HISTORY
Atomic absorption spectroscopy is deals with the
absorption of specific wave length of radiation by
neutral atoms in the ground state.
 The first atomic absorption spectroscopy was
built by CSIRO scientist Alan Walsh & Co-
Workers in Melbourne in 1954
 The first commercial AAS was introduced in
1959. Alan Walsh
(1916-
1998)
INTRODUCTION
PRINCIPLE
When solution of metallic salt is sprayed on to a
flame, fine droplets are formed due to the thermal
energy of flame. The solvent in the flame is
evaporated, leaving fine residue, which are
converted to neutral atoms. These neutral atoms
absorb radiation of specific wavelength emitted by
Hollow Cathode Lamp.
For the determination of every element, specific
Hollow Cathode Lamp is used.
LIQUID
SAMPLE
FORMATION
OF
DROPLETS
FINE
RESIDU
E
FORMATION
OF
NEUTRAL
ATOMS
NEUTRAL ATOMS
ABSORB SPECIFIC
WAVELENGTH OF
RADIATION
MEASUREMENT OF
INTENSITY OF RADIATION
BY USING PHOTOMETRIC
DETECTOR
FIG: Instrumental setup for atomic absorption
spectroscopy
INSTRUMENTATION
1. LIGHT SOURCE
 The light source is usually a Hollow
Cathode Lamp of the element that is
being measured.
 It consist a tungsten anode and a hollow
cylindrical cathode made of the element
to be determined.
 These are sealed in a glass tube filled
with an inert gas (Neon OR Argon). Each
element has its own unique lamp which
must be used for that analysis.
Fig: HOLLOW CATHODE
LAMP
FLAME
Temperature profile when fuel is Natural Gas and oxidant is Air
2. ATOMIZER
Atomization is done by exposing the analyte to high temperature in a
flame or electro thermal atomizer.
(i). Flame Atomizer
The most common way is to use a flame which is used for converting
the liquid sample into the gaseous state.
There are two types of flame atomizer;
(a).Total Consumption Burner
(b).Premixed Burner
Types of atomizer
There are two types of atomizer
(a). Total Consumption burner (b). Premixed burner
(ii). Electro thermal atomizer
In electro thermal atomizer firstly
sample is evaporated at a low
temperature and then it is
converted into ash by increasing
the temperature after ashing, the
temperature is increased from
2000-3000C. Atomization of
sample occur in few seconds
Commonly used electro thermal
atomizer is Graphite tube.
Graphite tube
HCL
3. SAMPEL INTRODUCTION SYSTEM
In atomizer, sample is constantly introduced in form of droplets.
NEBULIZER:
A device for converting sample into fine spray or droplets.
Capillary
Nebuliser
Sample Solution
4. MONOCHROMATER
Monochromater is used to separate out all of the thousand of
lines.
In AAS Prism OR Grating system is used as a monochromater.
5. DETECTORS
For AAS Photomultiplier tube is used.
6. READOUT DEVICE
In AAS, measurement chart recorder is used as readout device.
INTERFERENCES
Different types of interferences which affect AAS are as
follows-
1. Spectral Interference
2. Chemical Interference
3. Ionization Interference
4. Solvent Interference
ADVANTAGES
Equipment relatively cheap
Easy to use (training easy compared to
furnace)
Good precision
High sample throughput
Relatively easy method development
Cheap to run
DISADVANTAGES
Lack of sensitivity (compared to furnace)
Problems with refractory elements
Require large sample size
Sample must be in solution
APPLICATIONS
1. Qualitative Analysis
2. Quantitative Analysis
3. Estimation of trace elements in biological fluid
like blood, urine, etc.
4. Estimation of trace elements like Copper , Nickel
and Zinc in food products.
5. Estimation of Lead in petrol.
6. Estimation of elements in soil samples , water
supply , effluents , ceramics , etc.
REFERENCES
Principles of Instrumental Analysis, 6th Edition
(Douglas A. skoog)
Instrumental Methods of Chemical analysis 5th Edition
(Gurdeep R. Chatwal)
The application of Atomic Absorption Spectra to Chemical
analysis (A. Walsh (1995))
Wikipedia
Presentation1

More Related Content

What's hot

Atomic Absorption Spectrophotometer (AAS) by ACMAS Technologies Pvt Ltd.
Atomic Absorption Spectrophotometer (AAS) by ACMAS Technologies Pvt Ltd.Atomic Absorption Spectrophotometer (AAS) by ACMAS Technologies Pvt Ltd.
Atomic Absorption Spectrophotometer (AAS) by ACMAS Technologies Pvt Ltd.
Acmas Technologies Pvt. Ltd.
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
akshay raghavendra
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
Kalsoom Mohammed
 
7 atomic absorption spectroscopy
7 atomic absorption spectroscopy7 atomic absorption spectroscopy
7 atomic absorption spectroscopy
Frank Loh
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
Sadiq Rahim
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
Sangam Kanthale
 
Atomic absorption spectrometry (aas)
Atomic absorption spectrometry (aas)Atomic absorption spectrometry (aas)
Atomic absorption spectrometry (aas)
Maulana Azad College of Engineering and Technology
 
Atomic absorption spectrophotometry
Atomic absorption spectrophotometryAtomic absorption spectrophotometry
Atomic absorption spectrophotometry
Govardhan Joshi
 
Vip atomic absorption spectroscopy
Vip atomic absorption spectroscopyVip atomic absorption spectroscopy
Vip atomic absorption spectroscopy
vipendra singh
 
ATOMIC ABSORPTION SPECTROSCOPY
ATOMIC ABSORPTION SPECTROSCOPYATOMIC ABSORPTION SPECTROSCOPY
ATOMIC ABSORPTION SPECTROSCOPY
SUBHASISH DAS
 
Atomic spectroscopy (AAS & AES)
Atomic spectroscopy  (AAS & AES) Atomic spectroscopy  (AAS & AES)
Atomic spectroscopy (AAS & AES)
Rohan Jagdale
 
Flame Atomic Absorption Spectroscopy
Flame Atomic Absorption SpectroscopyFlame Atomic Absorption Spectroscopy
Flame Atomic Absorption Spectroscopy
Haydar Mohammad Salim
 
atomic absorption spectroscopy
atomic absorption spectroscopyatomic absorption spectroscopy
atomic absorption spectroscopy
JyotiMhoprekar
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
Ajay Dominic
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
Saptarshi Das
 
Atomic absorption spectroscopy
Atomic absorption spectroscopy Atomic absorption spectroscopy
Atomic absorption spectroscopy
Mayur Bodhankar
 
Q canalytic aas2
Q canalytic aas2Q canalytic aas2
Q canalytic aas2
Pepay Galicia
 
Flame Spectroscopy Analysis B.Pharma and M/Pharma
Flame Spectroscopy Analysis B.Pharma and M/PharmaFlame Spectroscopy Analysis B.Pharma and M/Pharma
Flame Spectroscopy Analysis B.Pharma and M/Pharma
AmanKapoorMPharma
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
chemistrypriyanka
 
Presentation1
Presentation1Presentation1
Presentation1
MARYAM ANSAR
 

What's hot (20)

Atomic Absorption Spectrophotometer (AAS) by ACMAS Technologies Pvt Ltd.
Atomic Absorption Spectrophotometer (AAS) by ACMAS Technologies Pvt Ltd.Atomic Absorption Spectrophotometer (AAS) by ACMAS Technologies Pvt Ltd.
Atomic Absorption Spectrophotometer (AAS) by ACMAS Technologies Pvt Ltd.
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
 
7 atomic absorption spectroscopy
7 atomic absorption spectroscopy7 atomic absorption spectroscopy
7 atomic absorption spectroscopy
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
 
Atomic absorption spectrometry (aas)
Atomic absorption spectrometry (aas)Atomic absorption spectrometry (aas)
Atomic absorption spectrometry (aas)
 
Atomic absorption spectrophotometry
Atomic absorption spectrophotometryAtomic absorption spectrophotometry
Atomic absorption spectrophotometry
 
Vip atomic absorption spectroscopy
Vip atomic absorption spectroscopyVip atomic absorption spectroscopy
Vip atomic absorption spectroscopy
 
ATOMIC ABSORPTION SPECTROSCOPY
ATOMIC ABSORPTION SPECTROSCOPYATOMIC ABSORPTION SPECTROSCOPY
ATOMIC ABSORPTION SPECTROSCOPY
 
Atomic spectroscopy (AAS & AES)
Atomic spectroscopy  (AAS & AES) Atomic spectroscopy  (AAS & AES)
Atomic spectroscopy (AAS & AES)
 
Flame Atomic Absorption Spectroscopy
Flame Atomic Absorption SpectroscopyFlame Atomic Absorption Spectroscopy
Flame Atomic Absorption Spectroscopy
 
atomic absorption spectroscopy
atomic absorption spectroscopyatomic absorption spectroscopy
atomic absorption spectroscopy
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
 
Atomic absorption spectroscopy
Atomic absorption spectroscopy Atomic absorption spectroscopy
Atomic absorption spectroscopy
 
Q canalytic aas2
Q canalytic aas2Q canalytic aas2
Q canalytic aas2
 
Flame Spectroscopy Analysis B.Pharma and M/Pharma
Flame Spectroscopy Analysis B.Pharma and M/PharmaFlame Spectroscopy Analysis B.Pharma and M/Pharma
Flame Spectroscopy Analysis B.Pharma and M/Pharma
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
 
Presentation1
Presentation1Presentation1
Presentation1
 

Similar to Presentation1

ATOMIC ABSORPTION SPECTROSCOPY
ATOMIC ABSORPTION SPECTROSCOPYATOMIC ABSORPTION SPECTROSCOPY
ATOMIC ABSORPTION SPECTROSCOPY
Qurratulain Shams Rizvi
 
Atomic absorption spectroscopy
Atomic absorption spectroscopy Atomic absorption spectroscopy
Atomic absorption spectroscopy
Zainab&Sons
 
Atomic absorption spectroscopy lec
Atomic absorption spectroscopy lecAtomic absorption spectroscopy lec
Atomic absorption spectroscopy lec
Zainab&Sons
 
Atomic Absorption Spectroscopy (www.Redicals.com)
Atomic Absorption Spectroscopy (www.Redicals.com)Atomic Absorption Spectroscopy (www.Redicals.com)
Atomic Absorption Spectroscopy (www.Redicals.com)
Goa App
 
Mridu- AAS.pptx
Mridu- AAS.pptxMridu- AAS.pptx
Mridu- AAS.pptx
SweetuCutie
 
Atomic Absorption Spectroscopy (AAS)
Atomic Absorption Spectroscopy (AAS)Atomic Absorption Spectroscopy (AAS)
Atomic Absorption Spectroscopy (AAS)
Parijat Suryawanshi
 
Atomic absorption
Atomic absorption Atomic absorption
Atomic absorption
Qayoom Mugheri
 
Aas
AasAas
flame photometery
flame photometeryflame photometery
flame photometery
KarthikSwamybm
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
Sharmi143
 
Atomic Absorption Spectrometer.pptx
Atomic Absorption Spectrometer.pptxAtomic Absorption Spectrometer.pptx
Atomic Absorption Spectrometer.pptx
drpvczback
 
Atomic absorption spectroscopy (AAS).pptx
Atomic absorption spectroscopy (AAS).pptxAtomic absorption spectroscopy (AAS).pptx
Atomic absorption spectroscopy (AAS).pptx
Priyankashah645680
 
Flame emission & atomic absorption spectroscopy
Flame emission & atomic absorption spectroscopyFlame emission & atomic absorption spectroscopy
Flame emission & atomic absorption spectroscopy
Himal Barakoti
 
Chetan
ChetanChetan
Atomic absorption Spectrophotometry
Atomic absorption Spectrophotometry Atomic absorption Spectrophotometry
Atomic absorption Spectrophotometry
Hari Sharan Makaju
 
1606732404-atomic-absorption-emission-2.ppt
1606732404-atomic-absorption-emission-2.ppt1606732404-atomic-absorption-emission-2.ppt
1606732404-atomic-absorption-emission-2.ppt
RanaNoman21
 
Atomic Absorption Techniques & Applications
Atomic Absorption  Techniques & ApplicationsAtomic Absorption  Techniques & Applications
Atomic Absorption Techniques & Applications
Gamal Abdel Hamid
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
Salah Ud Din Shabab
 
ATOMIC ABSORPTION SPECTROSCOPY by Faizan Akram
ATOMIC ABSORPTION SPECTROSCOPY by Faizan AkramATOMIC ABSORPTION SPECTROSCOPY by Faizan Akram
ATOMIC ABSORPTION SPECTROSCOPY by Faizan Akram
Faizan Akram
 
ATOMIC ABSORPTION SPECTROPHOTOMETRY
ATOMIC ABSORPTION SPECTROPHOTOMETRYATOMIC ABSORPTION SPECTROPHOTOMETRY
ATOMIC ABSORPTION SPECTROPHOTOMETRY
Raju Sanghvi
 

Similar to Presentation1 (20)

ATOMIC ABSORPTION SPECTROSCOPY
ATOMIC ABSORPTION SPECTROSCOPYATOMIC ABSORPTION SPECTROSCOPY
ATOMIC ABSORPTION SPECTROSCOPY
 
Atomic absorption spectroscopy
Atomic absorption spectroscopy Atomic absorption spectroscopy
Atomic absorption spectroscopy
 
Atomic absorption spectroscopy lec
Atomic absorption spectroscopy lecAtomic absorption spectroscopy lec
Atomic absorption spectroscopy lec
 
Atomic Absorption Spectroscopy (www.Redicals.com)
Atomic Absorption Spectroscopy (www.Redicals.com)Atomic Absorption Spectroscopy (www.Redicals.com)
Atomic Absorption Spectroscopy (www.Redicals.com)
 
Mridu- AAS.pptx
Mridu- AAS.pptxMridu- AAS.pptx
Mridu- AAS.pptx
 
Atomic Absorption Spectroscopy (AAS)
Atomic Absorption Spectroscopy (AAS)Atomic Absorption Spectroscopy (AAS)
Atomic Absorption Spectroscopy (AAS)
 
Atomic absorption
Atomic absorption Atomic absorption
Atomic absorption
 
Aas
AasAas
Aas
 
flame photometery
flame photometeryflame photometery
flame photometery
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
 
Atomic Absorption Spectrometer.pptx
Atomic Absorption Spectrometer.pptxAtomic Absorption Spectrometer.pptx
Atomic Absorption Spectrometer.pptx
 
Atomic absorption spectroscopy (AAS).pptx
Atomic absorption spectroscopy (AAS).pptxAtomic absorption spectroscopy (AAS).pptx
Atomic absorption spectroscopy (AAS).pptx
 
Flame emission & atomic absorption spectroscopy
Flame emission & atomic absorption spectroscopyFlame emission & atomic absorption spectroscopy
Flame emission & atomic absorption spectroscopy
 
Chetan
ChetanChetan
Chetan
 
Atomic absorption Spectrophotometry
Atomic absorption Spectrophotometry Atomic absorption Spectrophotometry
Atomic absorption Spectrophotometry
 
1606732404-atomic-absorption-emission-2.ppt
1606732404-atomic-absorption-emission-2.ppt1606732404-atomic-absorption-emission-2.ppt
1606732404-atomic-absorption-emission-2.ppt
 
Atomic Absorption Techniques & Applications
Atomic Absorption  Techniques & ApplicationsAtomic Absorption  Techniques & Applications
Atomic Absorption Techniques & Applications
 
Atomic absorption spectroscopy
Atomic absorption spectroscopyAtomic absorption spectroscopy
Atomic absorption spectroscopy
 
ATOMIC ABSORPTION SPECTROSCOPY by Faizan Akram
ATOMIC ABSORPTION SPECTROSCOPY by Faizan AkramATOMIC ABSORPTION SPECTROSCOPY by Faizan Akram
ATOMIC ABSORPTION SPECTROSCOPY by Faizan Akram
 
ATOMIC ABSORPTION SPECTROPHOTOMETRY
ATOMIC ABSORPTION SPECTROPHOTOMETRYATOMIC ABSORPTION SPECTROPHOTOMETRY
ATOMIC ABSORPTION SPECTROPHOTOMETRY
 

Recently uploaded

NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...
NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...
NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...
Payaamvohra1
 
Haunted Houses by H W Longfellow for class 10
Haunted Houses by H W Longfellow for class 10Haunted Houses by H W Longfellow for class 10
Haunted Houses by H W Longfellow for class 10
nitinpv4ai
 
Observational Learning
Observational Learning Observational Learning
Observational Learning
sanamushtaq922
 
skeleton System.pdf (skeleton system wow)
skeleton System.pdf (skeleton system wow)skeleton System.pdf (skeleton system wow)
skeleton System.pdf (skeleton system wow)
Mohammad Al-Dhahabi
 
A Free 200-Page eBook ~ Brain and Mind Exercise.pptx
A Free 200-Page eBook ~ Brain and Mind Exercise.pptxA Free 200-Page eBook ~ Brain and Mind Exercise.pptx
A Free 200-Page eBook ~ Brain and Mind Exercise.pptx
OH TEIK BIN
 
CIS 4200-02 Group 1 Final Project Report (1).pdf
CIS 4200-02 Group 1 Final Project Report (1).pdfCIS 4200-02 Group 1 Final Project Report (1).pdf
CIS 4200-02 Group 1 Final Project Report (1).pdf
blueshagoo1
 
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
Nguyen Thanh Tu Collection
 
Geography as a Discipline Chapter 1 __ Class 11 Geography NCERT _ Class Notes...
Geography as a Discipline Chapter 1 __ Class 11 Geography NCERT _ Class Notes...Geography as a Discipline Chapter 1 __ Class 11 Geography NCERT _ Class Notes...
Geography as a Discipline Chapter 1 __ Class 11 Geography NCERT _ Class Notes...
ImMuslim
 
Pharmaceutics Pharmaceuticals best of brub
Pharmaceutics Pharmaceuticals best of brubPharmaceutics Pharmaceuticals best of brub
Pharmaceutics Pharmaceuticals best of brub
danielkiash986
 
Data Structure using C by Dr. K Adisesha .ppsx
Data Structure using C by Dr. K Adisesha .ppsxData Structure using C by Dr. K Adisesha .ppsx
Data Structure using C by Dr. K Adisesha .ppsx
Prof. Dr. K. Adisesha
 
Standardized tool for Intelligence test.
Standardized tool for Intelligence test.Standardized tool for Intelligence test.
Standardized tool for Intelligence test.
deepaannamalai16
 
220711130083 SUBHASHREE RAKSHIT Internet resources for social science
220711130083 SUBHASHREE RAKSHIT  Internet resources for social science220711130083 SUBHASHREE RAKSHIT  Internet resources for social science
220711130083 SUBHASHREE RAKSHIT Internet resources for social science
Kalna College
 
Skimbleshanks-The-Railway-Cat by T S Eliot
Skimbleshanks-The-Railway-Cat by T S EliotSkimbleshanks-The-Railway-Cat by T S Eliot
Skimbleshanks-The-Railway-Cat by T S Eliot
nitinpv4ai
 
Temple of Asclepius in Thrace. Excavation results
Temple of Asclepius in Thrace. Excavation resultsTemple of Asclepius in Thrace. Excavation results
Temple of Asclepius in Thrace. Excavation results
Krassimira Luka
 
Bonku-Babus-Friend by Sathyajith Ray (9)
Bonku-Babus-Friend by Sathyajith Ray  (9)Bonku-Babus-Friend by Sathyajith Ray  (9)
Bonku-Babus-Friend by Sathyajith Ray (9)
nitinpv4ai
 
BIOLOGY NATIONAL EXAMINATION COUNCIL (NECO) 2024 PRACTICAL MANUAL.pptx
BIOLOGY NATIONAL EXAMINATION COUNCIL (NECO) 2024 PRACTICAL MANUAL.pptxBIOLOGY NATIONAL EXAMINATION COUNCIL (NECO) 2024 PRACTICAL MANUAL.pptx
BIOLOGY NATIONAL EXAMINATION COUNCIL (NECO) 2024 PRACTICAL MANUAL.pptx
RidwanHassanYusuf
 
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptxCapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
CapitolTechU
 
Andreas Schleicher presents PISA 2022 Volume III - Creative Thinking - 18 Jun...
Andreas Schleicher presents PISA 2022 Volume III - Creative Thinking - 18 Jun...Andreas Schleicher presents PISA 2022 Volume III - Creative Thinking - 18 Jun...
Andreas Schleicher presents PISA 2022 Volume III - Creative Thinking - 18 Jun...
EduSkills OECD
 
220711130088 Sumi Basak Virtual University EPC 3.pptx
220711130088 Sumi Basak Virtual University EPC 3.pptx220711130088 Sumi Basak Virtual University EPC 3.pptx
220711130088 Sumi Basak Virtual University EPC 3.pptx
Kalna College
 
How to Manage Reception Report in Odoo 17
How to Manage Reception Report in Odoo 17How to Manage Reception Report in Odoo 17
How to Manage Reception Report in Odoo 17
Celine George
 

Recently uploaded (20)

NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...
NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...
NIPER 2024 MEMORY BASED QUESTIONS.ANSWERS TO NIPER 2024 QUESTIONS.NIPER JEE 2...
 
Haunted Houses by H W Longfellow for class 10
Haunted Houses by H W Longfellow for class 10Haunted Houses by H W Longfellow for class 10
Haunted Houses by H W Longfellow for class 10
 
Observational Learning
Observational Learning Observational Learning
Observational Learning
 
skeleton System.pdf (skeleton system wow)
skeleton System.pdf (skeleton system wow)skeleton System.pdf (skeleton system wow)
skeleton System.pdf (skeleton system wow)
 
A Free 200-Page eBook ~ Brain and Mind Exercise.pptx
A Free 200-Page eBook ~ Brain and Mind Exercise.pptxA Free 200-Page eBook ~ Brain and Mind Exercise.pptx
A Free 200-Page eBook ~ Brain and Mind Exercise.pptx
 
CIS 4200-02 Group 1 Final Project Report (1).pdf
CIS 4200-02 Group 1 Final Project Report (1).pdfCIS 4200-02 Group 1 Final Project Report (1).pdf
CIS 4200-02 Group 1 Final Project Report (1).pdf
 
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
CHUYÊN ĐỀ ÔN TẬP VÀ PHÁT TRIỂN CÂU HỎI TRONG ĐỀ MINH HỌA THI TỐT NGHIỆP THPT ...
 
Geography as a Discipline Chapter 1 __ Class 11 Geography NCERT _ Class Notes...
Geography as a Discipline Chapter 1 __ Class 11 Geography NCERT _ Class Notes...Geography as a Discipline Chapter 1 __ Class 11 Geography NCERT _ Class Notes...
Geography as a Discipline Chapter 1 __ Class 11 Geography NCERT _ Class Notes...
 
Pharmaceutics Pharmaceuticals best of brub
Pharmaceutics Pharmaceuticals best of brubPharmaceutics Pharmaceuticals best of brub
Pharmaceutics Pharmaceuticals best of brub
 
Data Structure using C by Dr. K Adisesha .ppsx
Data Structure using C by Dr. K Adisesha .ppsxData Structure using C by Dr. K Adisesha .ppsx
Data Structure using C by Dr. K Adisesha .ppsx
 
Standardized tool for Intelligence test.
Standardized tool for Intelligence test.Standardized tool for Intelligence test.
Standardized tool for Intelligence test.
 
220711130083 SUBHASHREE RAKSHIT Internet resources for social science
220711130083 SUBHASHREE RAKSHIT  Internet resources for social science220711130083 SUBHASHREE RAKSHIT  Internet resources for social science
220711130083 SUBHASHREE RAKSHIT Internet resources for social science
 
Skimbleshanks-The-Railway-Cat by T S Eliot
Skimbleshanks-The-Railway-Cat by T S EliotSkimbleshanks-The-Railway-Cat by T S Eliot
Skimbleshanks-The-Railway-Cat by T S Eliot
 
Temple of Asclepius in Thrace. Excavation results
Temple of Asclepius in Thrace. Excavation resultsTemple of Asclepius in Thrace. Excavation results
Temple of Asclepius in Thrace. Excavation results
 
Bonku-Babus-Friend by Sathyajith Ray (9)
Bonku-Babus-Friend by Sathyajith Ray  (9)Bonku-Babus-Friend by Sathyajith Ray  (9)
Bonku-Babus-Friend by Sathyajith Ray (9)
 
BIOLOGY NATIONAL EXAMINATION COUNCIL (NECO) 2024 PRACTICAL MANUAL.pptx
BIOLOGY NATIONAL EXAMINATION COUNCIL (NECO) 2024 PRACTICAL MANUAL.pptxBIOLOGY NATIONAL EXAMINATION COUNCIL (NECO) 2024 PRACTICAL MANUAL.pptx
BIOLOGY NATIONAL EXAMINATION COUNCIL (NECO) 2024 PRACTICAL MANUAL.pptx
 
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptxCapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
CapTechTalks Webinar Slides June 2024 Donovan Wright.pptx
 
Andreas Schleicher presents PISA 2022 Volume III - Creative Thinking - 18 Jun...
Andreas Schleicher presents PISA 2022 Volume III - Creative Thinking - 18 Jun...Andreas Schleicher presents PISA 2022 Volume III - Creative Thinking - 18 Jun...
Andreas Schleicher presents PISA 2022 Volume III - Creative Thinking - 18 Jun...
 
220711130088 Sumi Basak Virtual University EPC 3.pptx
220711130088 Sumi Basak Virtual University EPC 3.pptx220711130088 Sumi Basak Virtual University EPC 3.pptx
220711130088 Sumi Basak Virtual University EPC 3.pptx
 
How to Manage Reception Report in Odoo 17
How to Manage Reception Report in Odoo 17How to Manage Reception Report in Odoo 17
How to Manage Reception Report in Odoo 17
 

Presentation1

  • 1. SOS IN ENVIRONMENTAL CHEMISTRY JIWAJI UNIVERSITY GWALIOR PRESENTATION ON – ATOMIC ABSORPTION SPECTROSCOPY Presented to Presented by Dr . NIMISHA JADON AKASH SAXENA (Assistant Professor) M.Sc. ICA (2nd SEM)
  • 3. HISTORY Atomic absorption spectroscopy is deals with the absorption of specific wave length of radiation by neutral atoms in the ground state.  The first atomic absorption spectroscopy was built by CSIRO scientist Alan Walsh & Co- Workers in Melbourne in 1954  The first commercial AAS was introduced in 1959. Alan Walsh (1916- 1998) INTRODUCTION
  • 4. PRINCIPLE When solution of metallic salt is sprayed on to a flame, fine droplets are formed due to the thermal energy of flame. The solvent in the flame is evaporated, leaving fine residue, which are converted to neutral atoms. These neutral atoms absorb radiation of specific wavelength emitted by Hollow Cathode Lamp. For the determination of every element, specific Hollow Cathode Lamp is used.
  • 5. LIQUID SAMPLE FORMATION OF DROPLETS FINE RESIDU E FORMATION OF NEUTRAL ATOMS NEUTRAL ATOMS ABSORB SPECIFIC WAVELENGTH OF RADIATION MEASUREMENT OF INTENSITY OF RADIATION BY USING PHOTOMETRIC DETECTOR
  • 6. FIG: Instrumental setup for atomic absorption spectroscopy INSTRUMENTATION
  • 7. 1. LIGHT SOURCE  The light source is usually a Hollow Cathode Lamp of the element that is being measured.  It consist a tungsten anode and a hollow cylindrical cathode made of the element to be determined.  These are sealed in a glass tube filled with an inert gas (Neon OR Argon). Each element has its own unique lamp which must be used for that analysis. Fig: HOLLOW CATHODE LAMP
  • 9. Temperature profile when fuel is Natural Gas and oxidant is Air
  • 10. 2. ATOMIZER Atomization is done by exposing the analyte to high temperature in a flame or electro thermal atomizer. (i). Flame Atomizer The most common way is to use a flame which is used for converting the liquid sample into the gaseous state. There are two types of flame atomizer; (a).Total Consumption Burner (b).Premixed Burner Types of atomizer There are two types of atomizer
  • 11. (a). Total Consumption burner (b). Premixed burner
  • 12. (ii). Electro thermal atomizer In electro thermal atomizer firstly sample is evaporated at a low temperature and then it is converted into ash by increasing the temperature after ashing, the temperature is increased from 2000-3000C. Atomization of sample occur in few seconds Commonly used electro thermal atomizer is Graphite tube. Graphite tube HCL
  • 13. 3. SAMPEL INTRODUCTION SYSTEM In atomizer, sample is constantly introduced in form of droplets. NEBULIZER: A device for converting sample into fine spray or droplets. Capillary Nebuliser Sample Solution
  • 14. 4. MONOCHROMATER Monochromater is used to separate out all of the thousand of lines. In AAS Prism OR Grating system is used as a monochromater. 5. DETECTORS For AAS Photomultiplier tube is used. 6. READOUT DEVICE In AAS, measurement chart recorder is used as readout device.
  • 15. INTERFERENCES Different types of interferences which affect AAS are as follows- 1. Spectral Interference 2. Chemical Interference 3. Ionization Interference 4. Solvent Interference
  • 16. ADVANTAGES Equipment relatively cheap Easy to use (training easy compared to furnace) Good precision High sample throughput Relatively easy method development Cheap to run
  • 17. DISADVANTAGES Lack of sensitivity (compared to furnace) Problems with refractory elements Require large sample size Sample must be in solution
  • 18. APPLICATIONS 1. Qualitative Analysis 2. Quantitative Analysis 3. Estimation of trace elements in biological fluid like blood, urine, etc. 4. Estimation of trace elements like Copper , Nickel and Zinc in food products. 5. Estimation of Lead in petrol. 6. Estimation of elements in soil samples , water supply , effluents , ceramics , etc.
  • 19. REFERENCES Principles of Instrumental Analysis, 6th Edition (Douglas A. skoog) Instrumental Methods of Chemical analysis 5th Edition (Gurdeep R. Chatwal) The application of Atomic Absorption Spectra to Chemical analysis (A. Walsh (1995)) Wikipedia