Introduction History Definition Structure of plasma membrane(fluid mosaic model) Examples to show dynamic nature of plasma membrane The diffusion of membrane protein after fusion Restriction on protein and mobility Control of membrane protein and mobility Membrane lipid mobility Membrane domains and cell polarity Function of plasma membrane Conclusion. References.

1. DYNAMIC STRUCTURE OF PLASMA MEMBRANE
)
By
KAUSHAL KUMAR SAHU
Assistant Professor (Ad Hoc)
Department of Biotechnology
Govt. Digvijay Autonomous P. G. College
Raj-Nandgaon ( C. G. )

2. •SYNOPSIS
 Introduction
 History
 Definition
 Structure of plasma membrane(fluid mosaic model)
 Examples to show dynamic nature of plasma membrane
o The diffusion of membrane protein after fusion
o Restriction on protein and mobility
o Control of membrane protein and mobility
o Membrane lipid mobility
o Membrane domains and cell polarity
 Function of plasma membrane
 Conclusion.
 References.

3. •INTRODUCTION
• The cell is the basic structural functional and
biological unit of all known living organism.
• Membranes are usually from 60 to 100 A thick .
• They are dynamic in nature any injured part of the
membrane is repaired with no time.

4. •HISTORY
• The plasma membrane was discovered by Schwann
1838.
• The term cell membrane was used by c.nageli and
c.cramer was 1855.
• The same was named “plasma lemma” was given
by j.o. plower 1931.

5. •DEFINATION
• Cell membrane is outer layer of protoplasm
which control ionic concentration between
protoplasm and atmosphere.

6. •STRUCTURE OF PLASMA MEMBRANE
(Fluid mosaic model)
• The essential feature of the fluid mosaic model is
that biological membrane considered to be classified
structure in which the lipid and integral protein are
arranged in mosaic manner .
• The fluid mosaic model of Singer & Nicolson is now
widely.

7. •HYDROPHOBIC INTERACTION

8. •THE DYNAMIC NATURE OF PLASMA
MEMBRANE
• Lipid bilayer can exit in a relatively fluid state.
• Lipid provide the matrix in which integral
proteins of a membrane are embedded the
physical state of the lipid is an important
determinant of the mobility of integral
protein.

9. •STRUCTURE

10. 1)EXAMPLE TO SHOW DYNAMIC NATURE
OF OF MEMBRANE PROTEIN
• Cell fusion is a technique where by two different
types of or cells from two different species can be
fused to produce one cell with a common cytoplasm
and a single continuous plasma membrane .
• The first experiments to demonstrate that
membrane proteins could move within the plane of
the membrane utilized cell fusion and they were
reported in 1970 by Larry Frye and Michel deaden of
Johns Hopkins university.

11. EXAMPLE TO SHOW DYNAMIC NATURE OF
PLASMA MEMBRANE PROTEIN
• In their experiments mouse and human cells were
fused and the locations of specific protein of the
plasma membrane followed once the two
membranes had become continuous .
• These early cell fusion experiments gave impression
that integral membrane proteins were capable of
virtually unrestricted movements .

12. STRUCTURE OF CELL FUSION

13. RESTRICTION ON PROTEIN AND LIPID
MOBILITY
• Several techniques allow researchers to follow the
movements of molecules in the membranes of living
cell using the light minerals in a fluorescence
recovery after photo bleaching

14. 2)RESTRICTION ON PROTEIN AND LIPID
MOBILITY
• In single particle tracking individual membrane
protein molecules are labeled usally with antibody
coated gold particle (approximately 40nm in
diameter) and the movements of the labeled
molecules are followed by computer enhanced
video microscopy .
• Some membrane proteins move randomly through -
out membrane though generally at rates
considerably less than would be measured in an
artificially lipid bilayer.
• Some membrane proteins fuil to move and
considered are to be immobilized

15. •Structure Measuring the diffusion rates of
membrane proteins by fluorescence recovery
after photo bleaching

16. 3)CONTROL OF MEMBRANE
PROTEIN AND MOBILITY
• The plasma membrane of many cells possess a febrile network
membrane of cell sifting of peripheral protein situated on the
cytoplasm sure face of the membrane certain proportion of a
membrane integral protein molecules are enter tethered to
the membrane skeleton or otherwise restricted by it.

17. •STRUCTURE OF MEMBRANE PROTEIN
MOBILITY

18. 4)MEMBRANE LIPID MOBILITY
• Computer analysis indicates that the phospholipids
diffuses freely within one compartment before it
jumps the “fence “ in to a neighboring compartment
and than over another fence in to an adjacent
compartment.

19. •STRUCTURE OF MEMBRANE LIPID
MOBILITY

20. 5)MEMBRANE DOMAINS IN CELL
POLARITY
• Most membrane however exhibit district variation in
protein composition and mobility ,especially in cells
whose various surface display different function .
• The epithelial cell that line intestinal wall or make up
the microscopic tubules of the kidney are highly
polarized cell whose different surface carry out
different function studies.

21. •STRUCTURE OF MEMBRANE DOMAINS AND
CELL POLARITY

22. •FUNCTION OF PLASMA MEMBRANE
• It gives shape to the cell .
• It helps in transportation of molecules.
• Receptors are present on the startle of membrane
which helps in transduction of different material.
• For signal transduction .
• It prevents the cell loss of macromolecule.
• It protects the cell from harmful substance.

23. •CONCLUSION
• Plasma membrane is mainly composed of lipid and
protein structure .
• It control the entrance and exit of molecules and ions
.
• It is very thin elastic and permeable membrane .
• Plasma membrane is essential for the maintenance
of cellular integrity which shows the dynamic
structure of the membrane .

24. •REFERANCES
• Gerald carp (sixth edition)cell &molecular biology .
• Geoffrey M.Cooper robert Hauseman –the cell a
molecular approach(2009)
• From net-(Wikipedia ./Wiki/cell membrane)
• Date and time (10.30am,10/9/2013)