This document describes the procedure for preparing adipic acid from cyclohexanol via oxidation. Cyclohexanol is oxidized using nitric acid and ammonium metavanadate catalyst at 65-70°C to yield adipic acid crystals. The theoretical and experimental yields and melting point of adipic acid are calculated and measured. Adipic acid is an important industrial dicarboxylic acid used to produce nylon polymers.
Solventless reaction in green chemistryAfrin Nirfa
Solventless reactions or solid state reactions are one of the principles involved in green chemistry. these reactions are more useful because the toxicity of solvents are reduced, easy to handle, cheaper and makes environment friendly.
Solventless reaction in green chemistryAfrin Nirfa
Solventless reactions or solid state reactions are one of the principles involved in green chemistry. these reactions are more useful because the toxicity of solvents are reduced, easy to handle, cheaper and makes environment friendly.
Synthesis And Characterization of Novel Processable Poly (EtherAzomethine)S C...inventionjournals
The novel dialdehyde 1,1-bis[4-(4-benzaldehyde oxy) phenyl] cyclopentane (III) (BBPC) was synthesized starting from cyclopentanone and phenol to give 1, 1-Bis (4-hydroxy phenyl) cyclopentane (II); followed by reaction with p- fluorobenzaldehyde in N, N-dimethyl formamide (DMAc), containing anhydrous potassium carbonate. New series of poly (ether – azomethine)s were synthesized from (BBPC) with different diamines such as 4, 4’- diamino diphenyl ether (ODA),4,4’-diaminodiphenyl methane(MDA),4-aminophenyl sulfone(SDA), p-phenylene diamines, etc. in DMAc with 5 wt% LiCl by solution polycondensation technique. Inherent viscosities of these polymers were in the range 0.19 to 0.42 dL/g indicating formation of moderate molecular weights. These polymers exhibited good solubility in various polar aprotic solvents such as Nmethylpyrrolidone (NMP), N, N-dimethylacetamide (DMAc) and N,N-dimethyl formamide (DMF), etc. X-Ray diffraction pattern of polymers showed that introduction of cardo cyclopentylidene moiety containing ether linkage would disrupt the chain regularity and packing, leading to amorphous nature. Thermal analysis by TGA showed excellent thermal stability of polymers. The structure- property correlation among these polyazomethines were studied; in view of their potential applications as high performance polymers.
Synthesis and Characterization of cyclohexylidene containing novel cardo pol...inventionjournals
International Journal of Engineering and Science Invention (IJESI) is an international journal intended for professionals and researchers in all fields of computer science and electronics. IJESI publishes research articles and reviews within the whole field Engineering Science and Technology, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
International Journal of Engineering Research and Applications (IJERA) is an open access online peer reviewed international journal that publishes research and review articles in the fields of Computer Science, Neural Networks, Electrical Engineering, Software Engineering, Information Technology, Mechanical Engineering, Chemical Engineering, Plastic Engineering, Food Technology, Textile Engineering, Nano Technology & science, Power Electronics, Electronics & Communication Engineering, Computational mathematics, Image processing, Civil Engineering, Structural Engineering, Environmental Engineering, VLSI Testing & Low Power VLSI Design etc.
Synthesis, Characterization and Study of Antioxidant Activities of Some New P...IJRES Journal
A series of substituted pyrazoline derivatives 5(a-c) have been synthesized by the reaction of substituted chalcones 4(a-c) with isatinhydrazide. The starting materials, chalcones were prepared by clasien schimidt condensation of appropriate 1-hydroxy-2-acetonaphthone with substituted aldehydes in the presence of sodium hydroxide and in poly ethylene glycol (PEG-400). The structures of the synthesized compounds were confirmed by IR, 1HNMR & Mass spectral data. The synthesized compounds were screened for Antioxidant Activity by DPPH method.
In this presentation I have mentioned whatever the possible relevant content is required for this method
Citation Is done at the end of slide.
Content is up to date & true to my belief.
Thanks & Best Regards.
Anurag Pandey
B.Pharm (FACULTY OF PHARMACY, INVERTIS UNIVERSITY)
M.Pharm (INSTITUTE OF PHARMACY, NIRMA UNIVERSITY)
Email :- anurag.dmk05@gmail.com
Theories of coordination compounds, CFSE, Bonding in octahedral and tetrahedral complex, color of transition metal complex, magnetic properties, selection rules, Nephelxeuatic effect, angular overlap model
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
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z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
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from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
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(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...
Oxidation of cyclohexanol or preparation of adipic acid
1. CPP-1.5 (1.1) Practical Organic Chemistry
DOS & R in Organic Chemistry, TUT Page 1
Prepared By
Dr. Krishnaswamy G
Faculty
DOS & R in Organic Chemistry
Tumkur University
Tumakuru
2. CPP-1.5 (1.1) Practical Organic Chemistry
DOS & R in Organic Chemistry, TUT Page 2
Oxidation of Cyclohexanol or Preparation of Adipic
acid from cyclohexanol
AIM:
To prepare Adipic acid from cyclohexanol by oxidation.
PRINCIPLE:
Adipic acid is a dicarboxylic acid with IUPAC name Hexane-1,6-dioic
acid with the formula (CH2)4(COOH)2. Fromm an industrial perspective,
it is the most important dicarboxylic acid used as monomer for the
production of nylon by polycondensation reaction with hexamethylene
diamine forming 6, 6,-nylon. Hence, the purpose of this experiment is to
synthesize Adipic acid through oxidation of cyclohexanol using 50%
nitric acid in presence of catalyst ammonium metavanadate.
CHEMICALS REQUIRED:
Cyclohexanol, Concentrated nitric acid, Ammonium metavanadate.
APPARATUS:
Conical flask, Thermometer, Beaker, Funnel, Measuring cylinder, Filter
paper.
PROCEDURE:
3. CPP-1.5 (1.1) Practical Organic Chemistry
DOS & R in Organic Chemistry, TUT Page 3
In a 250 mL round bottom flask or conical flask 15 mL of 50%
HNO3 was placed and the acid solution was heated on water bath
nearly to 65-70oC.
A pinch of ammonium metavanadate (~0.05 g) was added to hot
acid solution.
1 mL of cyclohexanol was added dropwise to the acid solution
maintaining the temperature at 65-70oC.
The reaction progress was indicated by the evolution of brown gas
i.e. oxides of nitrogen.
After complete addition of cyclohexanol, maintain the temperature
for 10 more minutes till evolution of brown gas ceases.
The reaction mixture was cooled to lab temperature and was then
placed in an ice bath. Crystallization of Adipic acid takes place as
light yellow/white crystals.
The light yellow/white crystals were then collected by filtration
and washed with cold 50% HNO3. (Do not wash with excess)
The light yellow/white crystals were then dried and weighed.
Calculate the yield and obtain melting point.
REACTION:
OH
COOH
COOH50 % HNO3
Cat. NH4VO3
65-70o
C
CALCULATIONS:
4. CPP-1.5 (1.1) Practical Organic Chemistry
DOS & R in Organic Chemistry, TUT Page 4
Compound Amount
Molecular
Weight
Moles Equivalent
Cyclohexanol 1 mL
100.1
D = 0.96 g/cm3
- -
50 % HNO3 15 mL - - -
NH4VO3
Pinch
(~0.05g)
- - -
THEORETICAL CALCULATION:
100.1 g of cyclohexanol gives 146.14 g of Adipic acid
1 g of cyclohexanol will forms X g of Adipic acid
Theoretical yield =
Practical yield =
% Yield = (Practical yield) / (Theoretical yield) X 100
CHEMICAL TESTS:
Reaction with NaHCO3
Cyclohexanol: No evolution of gas (-Ve)
Adipic Acid: Evolution of gas (+Ve)
Litmus test:
Cyclohexanol: Blue litmus remain to blue and red litmus remain to red
(Neutral)
Adipic Acid: Blue litmus remain turns to red and red litmus remain to
red (Acidic)
5. CPP-1.5 (1.1) Practical Organic Chemistry
DOS & R in Organic Chemistry, TUT Page 5
MECHANISM:
OH
50 % HNO3
OH2
NO3
-H2O
O
N
O
O
H
O
HNO2 HNOH2O
OHO
N
O
NOH
O
O
COOH
COOHVO3
H2O
Keto-Enol tautomerism
H
O
-HNO2
Oxidation
6. CPP-1.5 (1.1) Practical Organic Chemistry
DOS & R in Organic Chemistry, TUT Page 6
REPORT:
Structure
and name
of the
compound
Theoretical
yield
Practical
yield
Percentage
yield
Theoretical
Melting
point
Experimental
Melting
point
COOH
COOH
Adipic acid
152oC
REFERENCES:
1. https://www.scribd.com/doc/114328909/Oxidation-of-
Cyclohexanol-to-Adipic-Acid.
2. Organic Syntheses, Coll. Vol. 1, p.18 (1941); Vol. 5, p.9 (1925).
3. https://www.academia.edu/31380866/PREPARATION_OF_ADIP
IC_ACID_FROM_CYCLOHEXENE_Chem_126_4.doc.
