1. Oil spills pollute the environment and harm living things by releasing toxic chemicals. Microorganisms like bacteria and fungi can biodegrade oil through metabolic processes.
2. The biodegradation process involves microbes initially adding oxygen to break down the hydrocarbon molecules in oil into simpler compounds. Nutrients like nitrogen and phosphorus often limit biodegradation and must be supplemented.
3. Different types of hydrocarbons in oil degrade at different rates, with straight-chain alkanes degrading most easily and aromatic rings most resistant to breakdown. Both augmenting indigenous microbes and stimulating their growth through nutrient addition can help remediate oil spills biologically.
ABSTRACT
INTRODUCTION
METHODOLOGY
BIOREMEDIATION OF OIL SPILLS
CASE STUDY
CONCLUSION
Subtopics
Bio remediation in hot and cold environments
Use of Nitrogen fixing Bacteria
Bio remediation using fungi from soil samples
Bio remediation using bacteria and case studies
•Introduction of bioremediation: Bioremediation refers to the process of using microorganisms to remove the environmental pollutants i.e. toxic wastes found in soil, water, air etc.
•In situ bioremediation:
It involves a direct approach for the microbial
degradation of xenobiotics at the sites of pollution
(soil, ground water).
•Types of in situ bioremediation:
Natural attenuation.
Engineered in situ bioremediation.
- Bioventing, biosparging, bioslurping,
phytoremediation.
•Ex situ bioremediation:
Waste or toxic pollutants can be collected from the polluted sites and bioremediation can be carried out at a designated place or site.
• Types of ex situ bioremediation
Land farming, windrow, biopiles, bioreactors.
•Microorganisms use in bioremediation:
A number of naturally occurring marine microbes
such as Pseudomonas sp. is capable of degrading oil and other hydrocarbons.
•Factors affecting bioremediation:
Nutrient availability, moisture content, pH, temperature, contaminant availability.
•References:
Satyanarayana U. Biotechnology. BOOKS AND ALLIED (P) Ltd.
Sharma P.D. Environmental Microbiology. RASTOGI PUBLICATIONS.
Gupta P.K. Biotechnology and Genomics. RASTOGI PUBLICATIONS.
Dubey R.C. A Textbook of Biotechnology. S Chand And Company Ltd.
Dubey R.C. A Textbook of Microbiology. S Chand And Company Ltd.
Willey/Sherwood/Woolverton. Prescott’s Microbiology. McGRAW-HILL INTERNATIONAL EDITION.
www.sciencedirect.com/bioremediation.
Bioremediation of heavy metals pollution by Udaykumar Pankajkumar BhanushaliUdayBhanushali111
Mechanisms and techniques used for Bioremediation which includes phytoremediation, Bacterial & fungal bioremediation. Examples of heavy metal pollution
ABSTRACT
INTRODUCTION
METHODOLOGY
BIOREMEDIATION OF OIL SPILLS
CASE STUDY
CONCLUSION
Subtopics
Bio remediation in hot and cold environments
Use of Nitrogen fixing Bacteria
Bio remediation using fungi from soil samples
Bio remediation using bacteria and case studies
•Introduction of bioremediation: Bioremediation refers to the process of using microorganisms to remove the environmental pollutants i.e. toxic wastes found in soil, water, air etc.
•In situ bioremediation:
It involves a direct approach for the microbial
degradation of xenobiotics at the sites of pollution
(soil, ground water).
•Types of in situ bioremediation:
Natural attenuation.
Engineered in situ bioremediation.
- Bioventing, biosparging, bioslurping,
phytoremediation.
•Ex situ bioremediation:
Waste or toxic pollutants can be collected from the polluted sites and bioremediation can be carried out at a designated place or site.
• Types of ex situ bioremediation
Land farming, windrow, biopiles, bioreactors.
•Microorganisms use in bioremediation:
A number of naturally occurring marine microbes
such as Pseudomonas sp. is capable of degrading oil and other hydrocarbons.
•Factors affecting bioremediation:
Nutrient availability, moisture content, pH, temperature, contaminant availability.
•References:
Satyanarayana U. Biotechnology. BOOKS AND ALLIED (P) Ltd.
Sharma P.D. Environmental Microbiology. RASTOGI PUBLICATIONS.
Gupta P.K. Biotechnology and Genomics. RASTOGI PUBLICATIONS.
Dubey R.C. A Textbook of Biotechnology. S Chand And Company Ltd.
Dubey R.C. A Textbook of Microbiology. S Chand And Company Ltd.
Willey/Sherwood/Woolverton. Prescott’s Microbiology. McGRAW-HILL INTERNATIONAL EDITION.
www.sciencedirect.com/bioremediation.
Bioremediation of heavy metals pollution by Udaykumar Pankajkumar BhanushaliUdayBhanushali111
Mechanisms and techniques used for Bioremediation which includes phytoremediation, Bacterial & fungal bioremediation. Examples of heavy metal pollution
Crude oil degradation by microorganismsrajani prabhu
importance of microorganism in bioremediation of crude oil contaminated sites. Mechanism of degradation of crude oil,methods used,Examples of organisms.
Introduction
Type of pesticides
Advantage & disadvantages of pesticides
Degradation of pesticide
Microbial degradation of pesticides
Mode of microbial metabolism of pesticides
Strategies for biodegradation
Approaches for biodegradation of pesticide
Chemical reaction leading biodegradation of pesticide
Metabolism of pesticides by MO
Metabolism of DDT
These slides are in pure form and students helpful in future prospects. All slides contains a specific amount of data with no extraordinary burden on students.
Soil is an ecological niche contains all major groups of microorganism - bacteria, fungi, algae, protozoa and virus, but bacteria are most numerouse each play a vital role in the ecological diversity.
✓Waste water is a term that is used to describe waste material that includes....
Food scraps
Oil and soaps.
Human wastes.
Industrial wastes.
Sewage waste that is collected from urban areas.
WASTE WATER AND THEIR TREATMENT (PRIMARY, SECONDARY AND TERTIARY)
This ppt contains all types of Microbial Bioremediation methods . Everyone can understand clearly . Explaining with neat pictures and animation . Useful for presentation about Microbes in bioremediation . At last it contains a small animated video which helps to get clear view .
IntroductionDefinitionPescidesType of pesticidesFate of pesticides in environmentBiodegradation of pesticides in soil Criteria for biodegradation
Strategies for biodegradationDifferent approaches of biodegradationChemical reaction leading to biodegradationChanging the spectrum of toxicityExample of biodegradationAdvantageDisadvantage
Crude oil degradation by microorganismsrajani prabhu
importance of microorganism in bioremediation of crude oil contaminated sites. Mechanism of degradation of crude oil,methods used,Examples of organisms.
Introduction
Type of pesticides
Advantage & disadvantages of pesticides
Degradation of pesticide
Microbial degradation of pesticides
Mode of microbial metabolism of pesticides
Strategies for biodegradation
Approaches for biodegradation of pesticide
Chemical reaction leading biodegradation of pesticide
Metabolism of pesticides by MO
Metabolism of DDT
These slides are in pure form and students helpful in future prospects. All slides contains a specific amount of data with no extraordinary burden on students.
Soil is an ecological niche contains all major groups of microorganism - bacteria, fungi, algae, protozoa and virus, but bacteria are most numerouse each play a vital role in the ecological diversity.
✓Waste water is a term that is used to describe waste material that includes....
Food scraps
Oil and soaps.
Human wastes.
Industrial wastes.
Sewage waste that is collected from urban areas.
WASTE WATER AND THEIR TREATMENT (PRIMARY, SECONDARY AND TERTIARY)
This ppt contains all types of Microbial Bioremediation methods . Everyone can understand clearly . Explaining with neat pictures and animation . Useful for presentation about Microbes in bioremediation . At last it contains a small animated video which helps to get clear view .
IntroductionDefinitionPescidesType of pesticidesFate of pesticides in environmentBiodegradation of pesticides in soil Criteria for biodegradation
Strategies for biodegradationDifferent approaches of biodegradationChemical reaction leading to biodegradationChanging the spectrum of toxicityExample of biodegradationAdvantageDisadvantage
Environmental Microbiology: Microbial degradation of recalcitrant compoundsTejaswini Petkar
A brief presentation on 'Microbial degradation of recalcitrant compounds'- their classes,their sources, the microorganisms involved and their modes of degradation,
Biodegradation is the chemical dissolution of materials by bacteria or other biological means.
biodegradable simply means to be consumed by microorganisms and return to compounds found in nature
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
1. BIODEGRADATION OF OIL SPILLS
Dr. Esther Shoba R
Assistant Professor
Kristu Jayanti College
Bangalore
2. INTRODUCTION
• Oil spills are the exit of liquid petroleum to human activity, and is a form of
pollution.
• Oil Spills can harm living things because its chemical
constituents are poisonous.
• This can Trace organisms both from internal exposure to oil through
ingestion or inhalation and from external exposure through eye and skin
irritation.
• Oil can also smother some small species of fish or invertebrates and fur and
coat feathers, reducing birds, and mammals, ability to maintain their body
temperatures.
• A tarball is a blob of petroleum and remnants of oil pollution which has
been weathered after floating in the ocean.
• Tarballs are an aquatic pollutant in further environments, although they
can happen naturally and as such are not always associated with oil spills
some small
3. • Petroleum-based products are the major source of energy for industry and
daily life.
• Leaks and accidental spills occur regularly during the exploration,
production, refining, transport, and storage of petroleum and petroleum
products.
• The amount of natural crude oil seepage was estimated to be 600,000
metric tons per year with a range of uncertainty of 200,000 metric tons
per year
• Release of hydrocarbons into the environment whether accidentally or
due to human activities is a main cause of water and soil pollution
• Soil contamination with hydrocarbons causes extensive damage of local
system since accumulation of pollutants in animals and plant tissue may
cause death or mutations
4. • The technology commonly used for the soil remediation includes
mechanical, burying, evaporation, dispersion, and washing.
• However, these technologies are expensive and can lead to incomplete
decomposition of contaminants.
• Biodegradation by natural populations of microorganisms represents
one of the primary mechanisms by which petroleum and other
hydrocarbon pollutants can be removed from the environment and is
cheaper than other remediation technologies
• The success of oil spill bioremediation depends on one’s ability to
establish and maintain conditions that favor enhanced oil
biodegradation rates in the contaminated environment.
5. • One important requirement is the presence of microorganisms with the
appropriate metabolic capabilities.
• If these microorganisms are present, then optimal rates of growth and
hydrocarbon biodegradation can be sustained by ensuring that adequate
concentrations of nutrients and oxygen are present and that the pH is
between 6 and 9.
• The physical and chemical characteristics of the oil and oil surface area
are also important determinants of bioremediation success.
• There are the two main approaches to oil spill bioremediation: (a)
bioaugmentation, in which known oil-degrading bacteria are added to
supplement the existing microbial population, and (b) biostimulation, in
which the growth of indigenous oil degraders is stimulated by the
addition of nutrients or other growth-limiting cosubstrates.
6. Composition of oil
• Crude oil is made up of four main elements.
• It usually contains 84% to 87% carbon, 11% to 14% hydrogen, 0.1% to
8% sulfur, 0.1% to 1.8% nitrogen, and 1% to 1.5% oxygen
• Although there are sodium, nitrogen, and oxygen compounds in oil, the
most common molecules are hydrocarbons.
• There are three main groups of hydrocarbon molecules in crude oil;
aromatics, naphthenes, and alkanes
7. AROMATIC HYDROCARBONS
• The most difficult hydrocarbons for bacteria to biodegrade are
aromatic compounds.
• Aromatic compounds are double-bonded carbon rings. Some of them
also have attached chains of hydrocarbons.
• Very small (one or two ring) aromatics evaporate off of a spill or can be
biodegraded.
• Larger aromatics, however, resist biodegradation, and can persist in
the area of a spill for a long time
• The only way that they can be broken down is by photo oxidation, or
degradation by U.V. light.
• Aromatics are also the most toxic compounds in crude oil
8. NAPTHALENES and ALKANES
• Naphthenes are single-bonded, saturated hydrocarbon rings.
• Naphthenes can be biodegraded more easily than aromatics, but not
as quickly as alkanes because they contain more bonds
• Alkanes are straight or branched saturated hydrocarbons.
• They only contain single bonds, which is ideal for microbial degradation
because it does not take much energy to break apart the molecules,
compared to double-bonded molecules.
9. • They can be solids, liquids, or gases, depending on the number of carbon
atoms they contain.
• Alkanes with one to four carbon atoms are gases, also called volatile
compounds.
• During an oil spill, these compounds evaporate off of the slick and into the
air.
• Alkanes with five to sixteen carbon atoms are liquids. These form most of
the oil slick.
• They can be degraded relatively quickly by bacteria.
• The smaller the chain, the easier it is for bacteria to break it down.
• Alkanes with more that sixteen carbon atoms are solids, and are difficult for
bacteria to break down.
10. • The saturates, the aromatics, the asphaltenes (phenols, fatty acids,
ketones, esters, and porphyrins), and the resins (pyridines, quinolines,
carbazoles, sulfoxides, and amides)
• The susceptibility of hydrocarbons to microbial degradation can be
generally ranked as follows: linear alkanes >branched alkanes > small
aromatics >cyclic alkanes
11.
12. MICROORGANISMS USED IN BIODEGRADATION
• Hydrocarbons in the environment are biodegraded primarily by
bacteria, yeast, and fungi.
• The reported efficiency of biodegradation ranged from 6% to 82% for
soil fungi, 0.13% to 50% for soil bacteria, and 0.003% to 100% for
marine bacteria.
• Many scientists reported that mixed populations with overall broad
enzymatic capacities are required to degrade complex mixtures of
hydrocarbons such as crude oil in soil, fresh water, and marine
environments.
13. BACTERIA
• Bacteria are the most active agents in petroleum degradation, and they
work as primary degraders of spilled oil in environment.
• Several bacteria are even known to feed exclusively on hydrocarbons
• Acinetobacter sp. was found to be capable of utilizing n-alkanes of
chain length C10–C40 as a sole source of carbon.
• Bacterial genera, namely, Gordonia, Brevibacterium, Aeromicrobium,
Dietzia, Burkholderia, and Mycobacterium isolated from petroleum
contaminated soil proved to be the potential organisms for
hydrocarbon degradation.
• The degradation of poly-aromatic hydrocarbons by Sphingomonas is
reported
14. FUNGI
• Fungal genera,
namely, Amorphoteca, Neosartorya, Talaromyces, and Graphium and
yeast genera, namely, Candida, Yarrowia, and Pichia were isolated from
petroleum-contaminated soil and proved to be the potential organisms
for hydrocarbon degradation.
• terrestrial fungi,
namely, Aspergillus, Cephalosporium, and Pencillium which were also
found to be the potential degrader of crude oil hydrocarbons.
• The yeast species, namely, Candida lipolytica, Rhodotorula
mucilaginosa, Geotrichum sp, and Trichosporon mucoides isolated from
contaminated water were noted to degrade petroleum compounds
15. • Alga, Prototheca zopfi which was capable of utilizing crude oil and a
mixed hydrocarbon substrate and exhibited extensive degradation of n-
alkanes and isoalkanes as well as aromatic hydrocarbons.
• It is observed that nine cyanobacteria, five green algae, one red alga,
one brown alga, and two diatoms could oxidize naphthalene.
17. NUTRIENTS
• Nutrients are very important ingredients for successful biodegradation
of hydrocarbon pollutants especially nitrogen, phosphorus, and in
some cases iron.
• Some of these nutrients could become limiting factor thus affecting
the biodegradation processes.
• Atlas reported that when a major oil spill occurred in marine and
freshwater environments, the supply of carbon was significantly
increased and the availability of nitrogen and phosphorus generally
became the limiting factor for oil degradation.
• In marine environments, it was found to be more pronounced due to
low levels of nitrogen and phosphorous in seawater
18. Mechanism of Petroleum Hydrocarbon Degradation
• The most rapid and complete degradation of the majority of organic
pollutants is brought about under aerobic conditions.
• The initial intracellular attack of organic pollutants is an oxidative
process and the activation as well as incorporation of oxygen is the
enzymatic key reaction catalyzed by oxygenases and peroxidases.
• Peripheral degradation pathways convert organic pollutants step by
step into intermediates of the central intermediary metabolism, for
example, the tricarboxylic acid cycle.
• Biosynthesis of cell biomass occurs from the central precursor
metabolites, for example, acetyl-CoA, succinate, pyruvate.
• Sugars required for various biosyntheses and growth are synthesized
by gluconeogenesis.
19.
20. • The key step of hydrocarbon degradation is the addition of one oxygen
atom, in some cases, two oxygen atoms, to the hydrocarbon molecule,
which is then converted to an alkanol (in the case of aliphatic
hydrocarbons) or to a phenol (in the case of aromatic molecules).
• In some species, an epoxide is the first intermediate.
• This activation makes the hydrocarbon more soluble in water, marks a
reactive site, and introduces a reactive site for the next reactions.
• The reaction requires energy, which is typically generated via the
oxidation of a reduced biological intermediate such as NADH, which
itself is reoxidized by an electron acceptor.
21. • The main intermediates of the alkane degradation are fatty acids, which
are produced from the alkanols via aldehydes.
• These acids can be further decomposed by the pathway typical of
physiologica carboxylic acid degradation, in which the molecule is
shortened stepwise.
• However, fatty acids can also be excreted by the cells and accumulate in
the environment.
• Once released, they can produce ambiguous effects. On the one hand,
fatty acids can serve as a carbon source for bacteria of a community, thus
enhancing the hydrocarbon degradation.
• On the other hand, fatty acids (chain length 14 C) can inhibit growth and
hydrocarbon metabolism because they interfere with the cell membrane
22.
23. Biodegradation of n-alkanes: metabolism begins with the activity of a monooxygenase which introduces a hydroxyl group into
the aliphatic chain. [A]-monoterminal oxidation, [B]-biterminal oxidation, [C]- subterminal oxidation);
24. Biodegradation of aromatic hydrocarbons: metabolism begins with the activity of a monooxygenase [1] or a dioxygenase [2] which introduce one or
two atoms of oxygen; it can also begin with unspecific reactions [3]
25. Complete mineralization or the dioxygenase pathway
• This pathway is taken mainly by bacteria.
• The monoaromatic molecule or one ring of the polyaromatic system is
attacked by a dioxygenase, and the molecule is oxidized stepwise via
formation of a diol and subsequent ring cleavage.
• Pyruvate is one of the main intermediates of the pathway.
• The main products are biomass and carbon dioxide.
• An accumulation of dead-end products is rare and occurs mostly when
cells are deficient in their degradation pathway.
• The disadvantage of this pathway is that only ring systems of up to
four rings are mineralized.
• Systems with a higher number of rings seem to be recalcitrant
26. Unspecific oxidation via radical reactions
• It includes the attack of phenolic molecule structure by a nonspecific
action, thus also attacking other aromatic structures such as PAH.
• The type of cleavage product is not predictable.
• Frequent metabolites of PAHs are quinones, quinoles, and ring systems
with a ring number lower than that of the original substance.
• These compounds may be incorporated into sediments and alter the
sediment structure.
• The wood-destroying white rot fungi, e.g., have been shown to destroy
the structure of lignin via the activity of extracellular peroxidases and
phenol oxidases.
27. Anaerobic hydrocarbon degradation
• The metabolic routes of alkane degradation seem to function differently and
are not completely understood yet.
• However it includes terminal or sub terminal addition of a one-carbon
moiety or a fumarate molecule to the alkane as an activation mechanism.
• For aromatic molecules, it has been demonstrated that alkyl benzenes which
have a methyl group as a side chain undergo an enzymes addition of
fumarate, most likely via a radical mechanism.
• This was demonstrated for toluene.
• Alkyl benzenes with side chains of two or more carbon atoms are activated
by dehydrogenation of the side chain.
• This has been shown for ethyl- and propylbenzene
28. Proposed pathway for anaerobic degradation of n-alkanes; activation via addition of a C1-moiety
(subterminal carboxylation at C3).
29. Proposed pathways of anaerobic degradation of aromatic hydrocarbons; activation via addition of fumarate,
[1]—succinate.
30.
31. BIOSURFACTANTS
• Biosurfactants are surface-active substances synthesized by living cells.
•
• Interest in microbial surfactants has been steadily increasing in recent
years due to their diversity, environmentally friendly nature, possibility of
large-scale production, selectivity, performance under extreme conditions,
and potential applications in environmental protection.
• Biosurfactants enhance the emulsification of hydrocarbons, have the
potential to solubilize hydrocarbon contaminants, and increase their
availability for microbial degradation.
32. BIOSURFACTANTS
• The use of chemicals for the treatment of a hydrocarbon polluted site may
contaminate the environment with their by-products, whereas biological
treatment may efficiently destroy pollutants, while being biodegradable
themselves.
• Therefore, biosurfactant-producing microorganisms may play an important
role in the accelerated bioremediation of hydrocarbon-contaminated sites.
• When grown on hydrocarbon substrate as the carbon source, these
microorganisms synthesize a wide range of chemicals with surface activity,
such as glycolipid, phospholipid, and others.
• These chemicals are synthesized to emulsify the hydrocarbon substrate
and facilitate its transport into the cells.
• In some bacterial species such as Pseudomonas aeruginosa,
biosurfactants are also involved in a group motility behavior called
swarming motility
33. • Pseudomonads are the best known bacteria capable of utilizing
hydrocarbons as carbon and energy sources and producing
biosurfactants.
• Among Pseudomonas, P. aeruginosa is widely studied for the
production of glycolipid type biosurfactants.
• However, glycolipid type biosurfactants are also reported from some
other species like P. putida and P. chlororaphis.
• Biosurfactants increase the oil surface area and that amount of oil is
actually available for bacteria to utilize it.
• Biosurfactants can act as emulsifying agents by decreasing the surface
tension and forming micelles.
• The microdroplets encapsulated in the hydrophobic microbial cell
surface are taken inside and degraded.
34.
35. Alcanivorax borkumensis
• Alcanivorax borkumensis is a marine bacteria that can absorb and digest
linear and branched alkanes that are found in crude oil and its products
• A. borkumensis is a gram-negative bacteria, meaning that the bacteria
has an outer membrane of lipopolysaccharides, unlike gram positive
bacteria who do not possess this layer.
• It is also a rod-shaped bacterium that is aerobic (oxygen reliant)
• A. borkumensis is included in the genus Bacillus, which is a genus for
rod-shaped bacterium and is in the class Gammaproteobacteria,
meaning that it is a scientifically important bacteria
• Since A. borkumensis occurs naturally in unpolluted waters all over the
world (including freshwater), it has to have a source of energy.
36. • A particular study has found that strains of two of the most abundant
cyanobacteria in the ocean (Prochlorococcus and Synechococcus)
produce and accumulate hydrocarbons, particularly alkanes C15 and
C17.
• These alkanes are the energy source for A. borkumensis in unpolluted
water.
• A. borkumensis naturally flourishes after an oil spill because there is a
more abundant source of energy that can sustain a larger population.
• A. borkumensis also participates in wastewater treatment by being
foamed by Nocardia spp.
• A borkumensis breaks apart the bonds in hydrocarbons in oil that
have been exposed to the sea, using enzymes and oxygen found in
the seawater
37. • A. borkumensis creates enzymes AlkB1 and AlkB2.
• AlkB1 is involved with the direct reversal of alkylation damage,
specifically in single-stranded DNA.
• AlkB1 hydroxylases alkanes with 5 to 12 carbons, and AlkB2
hydroxylases alkanes with 8 to 16 carbons .
• The chain lengths with the most A. borkumensis growth are 14 to 19
carbon chains.
• A. borkumensis is able to outcompete other hydrocarbonoclastic
species of bacteria because it can break down such a wide range of
alkane chains
38. BIOFILMS
• A biofilm may be defined as an assemblage of microorganisms comprising
of microbial species attached to a biological or inert surface and encased
in a self-synthesized matrix comprising of water, proteins, carbohydrates
and extracellular DNA.
• It may be anticipated that different microbial species present in consortia
of biofilms each with different metabolic degradation pathway are
capable of degrading several pollutants either individually or collectively.
• Biofilm forming bacteria are adapted to survive and suited for
bioremediation as they compete with nutrients and oxygen and
observations of tolerance of biofilms towards harsh environment found
way in the process of bioremediation.
• Biofilm mediated remediation is environment friendly and cost effective
option for cleaning up environmental pollutants.
39. • Use of biofilms is efficient for bioremediation as biofilms absorb,
immobilize and degrade various environmental pollutants.
• Bacterial biofilms exist within indigenous populations near the heavily
contaminated sites to better persist, survive and manage the harsh
environment.