The document discusses two scientific articles about DNA replication. The first article describes an enzyme called NEIL1 that acts as a "cowcatcher" by binding to single-stranded DNA damage before replication to stop the process and allow for repair. The second article reveals new details about the mechanism of DNA unwinding during replication through cryo-electron microscopy images of protein complexes involved. Studying these replication processes has implications for understanding and treating cancer.
Identification of new molecular mechanism indicates new ways to block uncontrolled cell division
Published on August 1/2014
Study reveals intricate mechanisms involved in enzyme that governs DNA duplication
Published on August 4/2014
Identification of new molecular mechanism indicates new ways to block uncontrolled cell division
Published on August 1/2014
Study reveals intricate mechanisms involved in enzyme that governs DNA duplication
Published on August 4/2014
Evento organizado pelo Instituto de Estudos Avançados da USP, Polo Ribeirão Preto (IEA-RP).
Mais informações: http://www.iearp.blogspot.com.br/2012/09/perspectivas-sobre-aplicacoes-de_18.html
The complete genome sequences of a number of organisms, including mammals, have recently become available because of rapid advances in DNA sequencing technology. Nevertheless, the analysis of transcripts still plays a crucial role in bridging the gap between the genome and the proteome, particularly in mammals. Therefore, as a method for the analysis of transcripts, cDNA library construction is crucial, even in the post-genome sequencing era.
https://www.creative-biogene.com/Services/cDNA-Library-Construction-Service
cDNA library construction using mRNA which are derived from DNA. cDNA is formed from the reverse transcription of single stranded mRNA. cDNA contains only the exons, it donot not contains introns. The mRNA consists of poly A tail in which the tRNA and rRNA donot contains poly A tail. A short oligo nucleotide of Poly T is used to isolate mRNA seperately thereby single stranded mRNA is then converted into cDNA by using reverse transcriptase enzyme.
Evento organizado pelo Instituto de Estudos Avançados da USP, Polo Ribeirão Preto (IEA-RP).
Mais informações: http://www.iearp.blogspot.com.br/2012/09/perspectivas-sobre-aplicacoes-de_18.html
The complete genome sequences of a number of organisms, including mammals, have recently become available because of rapid advances in DNA sequencing technology. Nevertheless, the analysis of transcripts still plays a crucial role in bridging the gap between the genome and the proteome, particularly in mammals. Therefore, as a method for the analysis of transcripts, cDNA library construction is crucial, even in the post-genome sequencing era.
https://www.creative-biogene.com/Services/cDNA-Library-Construction-Service
cDNA library construction using mRNA which are derived from DNA. cDNA is formed from the reverse transcription of single stranded mRNA. cDNA contains only the exons, it donot not contains introns. The mRNA consists of poly A tail in which the tRNA and rRNA donot contains poly A tail. A short oligo nucleotide of Poly T is used to isolate mRNA seperately thereby single stranded mRNA is then converted into cDNA by using reverse transcriptase enzyme.
Paper Writing Service - HelpWriting.net 👈
✅ Quality
You get an original and high-quality paper based on extensive research. The completed work will be correctly formatted, referenced and tailored to your level of study.
✅ Confidentiality
We value your privacy. We do not disclose your personal information to any third party without your consent. Your payment data is also safely handled as you process the payment through a secured and verified payment processor.
✅ Originality
Every single order we deliver is written from scratch according to your instructions. We have zero tolerance for plagiarism, so all completed papers are unique and checked for plagiarism using a leading plagiarism detector.
✅ On-time delivery
We strive to deliver quality custom written papers before the deadline. That's why you don't have to worry about missing the deadline for submitting your assignment.
✅ Free revisions
You can ask to revise your paper as many times as you need until you're completely satisfied with the result. Provide notes about what needs to be changed, and we'll change it right away.
✅ 24/7 Support
From answering simple questions to solving any possible issues, we're always here to help you in chat and on the phone. We've got you covered at any time, day or night.
DNA is a highly complex, intricate and extraordinary macromolecule found within all living cells. DNA is a "biochemical noun" and can be defined as "...a self- .... In other words, DNA refers to the molecules inside cells that carry genetic information and pass it from one generation to the next. The scientific name for DNA .... Free Essays from 123 Help Me | acid (DNA) is a molecule, a material rather, that is present in almost all living organisms. It is self-replicating and is .... 17. 2. 2022 ... Dna is the genetic fabric which is present in all the cells of the body. This molecule present a few characteristics, as VNTR, special present .... 24. 4. 2020 ... Genetic ancestry testing holds the potential to identify the geographic origins of an individual's ancestors, ancestral lineages, and relatives, .... 25. 4. 2022 ... 2022 DNA Day Essay Contest: Full Essays · 1st Place: Man Tak Mindy Shie, Grade 12. Teacher: Dr. Siew Hwey Alice Tan School: Singapore .... DNA, or Deoxyribonucleic acid, is two self replicating biopolymer strands that contain biological information that is necessary for human life. DNA is what .... DNA, also known as deoxyribonucleic acid, is a genetic information-carrying molecule that is essential for the development, growth, and reproduction of all .... DNA stand for deoxyribonucleic acid. RNA stands for ribonucleic acid. They share some similarities, such as both being nucleic acids.. Free Essay: The Structure and Replication of DNA Introduction The ... The enzyme polymerase is used to add new nucleotides to the growing DNA strands.
DNA is a long polymer of simple units called nucleotides. Each one contains a phosphate group (acid component), a sugar group (neutral component) and a nitrogen base (basic component).
DNA Replicaiton Cause Cancer Essay
Dna Replication And The Cell Cycle
Dna Replication Research Paper
DNA Complications In Biology
The Replication Of DNA In Escherichia Coli
Dna Replication Essay examples
Dna Synthesis Case Study
Dna Replication Essay
Dna And Cloning Research Paper
Semi-Conservative Dna Replication Essay
Rosalind Franklin Dna Replication
Replication Of The Viral Dsdna
DNA Essay
The Process of DNA Replication Essay
Dna Synthesis
Dna And Sequence Of Dna Essay
Dna Rna And : Dna Replication
DNA replication is a semiconservative process. It means that each strand acts as a template for the synthesis of a new complementary strand. Therefore, this process takes us from one parent molecule to two daughter molecules, with each newly formed double helix containing one new and one old strand.
The Discovery of DNA Essay
DNA EXTRACTION Essay
DNA Essay
DNA Essay example
DNA Essay
Dna Replication Essay
Dna Replication Essay examples
Dna Essay
DNA Profiling Essay example
Dna And Sequence Of Dna Essay
Essay On DNA Discoveries
DNA Forensic Essay
DNA testing Essay
Dna And Protein Synthesis Essay
Dna Discovery Essay
Dna Editing Research Paper
Dna Essay
DNA Essay
Dna Essay
DevOps and Testing slides at DASA ConnectKari Kakkonen
My and Rik Marselis slides at 30.5.2024 DASA Connect conference. We discuss about what is testing, then what is agile testing and finally what is Testing in DevOps. Finally we had lovely workshop with the participants trying to find out different ways to think about quality and testing in different parts of the DevOps infinity loop.
In his public lecture, Christian Timmerer provides insights into the fascinating history of video streaming, starting from its humble beginnings before YouTube to the groundbreaking technologies that now dominate platforms like Netflix and ORF ON. Timmerer also presents provocative contributions of his own that have significantly influenced the industry. He concludes by looking at future challenges and invites the audience to join in a discussion.
SAP Sapphire 2024 - ASUG301 building better apps with SAP Fiori.pdfPeter Spielvogel
Building better applications for business users with SAP Fiori.
• What is SAP Fiori and why it matters to you
• How a better user experience drives measurable business benefits
• How to get started with SAP Fiori today
• How SAP Fiori elements accelerates application development
• How SAP Build Code includes SAP Fiori tools and other generative artificial intelligence capabilities
• How SAP Fiori paves the way for using AI in SAP apps
Climate Impact of Software Testing at Nordic Testing DaysKari Kakkonen
My slides at Nordic Testing Days 6.6.2024
Climate impact / sustainability of software testing discussed on the talk. ICT and testing must carry their part of global responsibility to help with the climat warming. We can minimize the carbon footprint but we can also have a carbon handprint, a positive impact on the climate. Quality characteristics can be added with sustainability, and then measured continuously. Test environments can be used less, and in smaller scale and on demand. Test techniques can be used in optimizing or minimizing number of tests. Test automation can be used to speed up testing.
PHP Frameworks: I want to break free (IPC Berlin 2024)Ralf Eggert
In this presentation, we examine the challenges and limitations of relying too heavily on PHP frameworks in web development. We discuss the history of PHP and its frameworks to understand how this dependence has evolved. The focus will be on providing concrete tips and strategies to reduce reliance on these frameworks, based on real-world examples and practical considerations. The goal is to equip developers with the skills and knowledge to create more flexible and future-proof web applications. We'll explore the importance of maintaining autonomy in a rapidly changing tech landscape and how to make informed decisions in PHP development.
This talk is aimed at encouraging a more independent approach to using PHP frameworks, moving towards a more flexible and future-proof approach to PHP development.
Essentials of Automations: The Art of Triggers and Actions in FMESafe Software
In this second installment of our Essentials of Automations webinar series, we’ll explore the landscape of triggers and actions, guiding you through the nuances of authoring and adapting workspaces for seamless automations. Gain an understanding of the full spectrum of triggers and actions available in FME, empowering you to enhance your workspaces for efficient automation.
We’ll kick things off by showcasing the most commonly used event-based triggers, introducing you to various automation workflows like manual triggers, schedules, directory watchers, and more. Plus, see how these elements play out in real scenarios.
Whether you’re tweaking your current setup or building from the ground up, this session will arm you with the tools and insights needed to transform your FME usage into a powerhouse of productivity. Join us to discover effective strategies that simplify complex processes, enhancing your productivity and transforming your data management practices with FME. Let’s turn complexity into clarity and make your workspaces work wonders!
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
Pushing the limits of ePRTC: 100ns holdover for 100 daysAdtran
At WSTS 2024, Alon Stern explored the topic of parametric holdover and explained how recent research findings can be implemented in real-world PNT networks to achieve 100 nanoseconds of accuracy for up to 100 days.
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 4. In this session, we will cover Test Manager overview along with SAP heatmap.
The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
Welcome to the first live UiPath Community Day Dubai! Join us for this unique occasion to meet our local and global UiPath Community and leaders. You will get a full view of the MEA region's automation landscape and the AI Powered automation technology capabilities of UiPath. Also, hosted by our local partners Marc Ellis, you will enjoy a half-day packed with industry insights and automation peers networking.
📕 Curious on our agenda? Wait no more!
10:00 Welcome note - UiPath Community in Dubai
Lovely Sinha, UiPath Community Chapter Leader, UiPath MVPx3, Hyper-automation Consultant, First Abu Dhabi Bank
10:20 A UiPath cross-region MEA overview
Ashraf El Zarka, VP and Managing Director MEA, UiPath
10:35: Customer Success Journey
Deepthi Deepak, Head of Intelligent Automation CoE, First Abu Dhabi Bank
11:15 The UiPath approach to GenAI with our three principles: improve accuracy, supercharge productivity, and automate more
Boris Krumrey, Global VP, Automation Innovation, UiPath
12:15 To discover how Marc Ellis leverages tech-driven solutions in recruitment and managed services.
Brendan Lingam, Director of Sales and Business Development, Marc Ellis
1. 'Cowcatcher' Enzyme
Fixes Single-Strand DNA
AND Key Step in
Molecular 'Dance' That
Duplicates DNA
Deciphered
CARMEN SOFIA TORRES DAZA
Medical Student
III semester
Medellín/UPB
2.
3.
4. DNA, or deoxyribonucleic acid, is the hereditary
material in humans and almost all other organisms.
An important property of DNA is that it can
replicate, or make copies of itself. Each strand of
DNA in the double helix can serve as a pattern for
duplicating the sequence of bases. This is critical
when cells divide because each new cell needs to
have an exact copy of the DNA present in the old
cell.
Before it can occur, the length of the DNA double
helix about to be copied must be unwound. In
addition, the two strands must be separated, much
like the two sides of a zipper, by breaking the weak
hydrogen bonds that link the paired bases. Once
the DNA strands have been unwound, they must
be held apart to expose the bases so that new
nucleotide partners can hydrogen-bond to them.
If in this important process is found an error , its
necessary stop the replication for correct the
implicated bases and then continue whit this.
6. When a cell divides, this cell exposes
its genome. Prior to cell division, this
DNA splits into two single strands and
these splits are so vulnerable to many
assaults.
This assaults can generates changes in
the genetic information and if the
assaults don´t correct will be generate
mutations…
this damage can produce cancers and
other illness to the people.
FIRST NEW
7. According with the authors, this
investigation helps us to imagine
the DNA separation during
replication as a zipper.
This zipper opens and expose it
bases, and the problem is that the
new strand is susceptible to
damage and lead to breaking it.
FIRST NEW
8. Was Founded!!! NEIL 1 : “The Cowcatcher” …
In a in vitro experiments, scientists founded a enzyme called NEIL 1 .
This enzyme actually rides in front of the replication process looking a single strand DNA damage.
For this reason was called “ cowcatcher”.
If the cowcatcher detect a damage the NEIL 1 binds to the damage site and "stop" the replication .
literally it works of this way: “There is a regresses and the two strands come back together which
allows repair of the damaged base in duplex DNA, replacing the damaged base with the
appropriate normal base.“
for understanding this, the author of the article, Sankar Mitra, makes an analogy for the
function of NEIL 1: he call it the cowcatcher by comparing it with the structure of a
“locomotive on it front side” that is used to take off the animals or debris out of the road.
“basically the cowcatcher is in front of the train of the replication to see if there is any
damage in front of it” “if there is any damaged found, the train moves back, repairs it and
then start to moving again”
FIRST NEW
9. PERSONAL OPINION:
this investigation is a
good example for
show as molecular
biology is becomes
today in the way to
new goals and
knowledge that help
to improve the life
quality of the
people.
FIRST NEW
10. Key Step in
Molecular 'Dance'
That Duplicates
DNA Deciphered
“science daily, July 14
2013”
SECOND NEW
11. This research, was published in
the journal Nature Structural &
Molecular Biology and give us
information about the some
key step-molecular through
images showing how the
enzyme that unwinds the DNA
double helix gets drawn to and
wrapped around its target. And,
also the details and mechanism
of DNA replication with
important implications in
current cáncer investigations.
SECOND NEW
12. The "origin recognition complex" (ORC), identifies and binds to DNA-
replication "start" sites, and it is a important factor in this is process
because this protein also interacts with the enzyme that eventually
unwinds the DNA double helix into two separate strands. There are a
kind of missing link- what happens to this helicase. This helicase needs
different protein components to generate a helicase loading complex.
SECOND NEW
13. They purified and then remixed together pieces of the protein including the
origin recognition complex, the replication initiator, the core of the helicase,
and other components and embedded them in ice, took pictures with a cryo-
electron microscope and reconstructe the 3-D structure in a computer. "The
3-D reconstruction gave their a snapshot of the elusive intermediate
structure. This intermediate structure is a very important part of the
molecular machinery that exist on fleeting timescales.
This investigations about the helicasa have important implications, possibly
pointing to new ways to fight cancer, because irregularities in DNA
duplication and uncontrolled cell division are hallmarks of the disease.
SECOND NEW
14. PERSONAL OPINION
I think in a way this article keeps
relation with the previous ,
because "stop" the cell replication
when it detects any damage is
very important to save the lives of
many people with many types of
cancers.
15. MEDICAL UTILITY
The DNA is contained all the genetic information of a living being. The study of DNA is
important in many subjects of the science but in medicine specially because we can know
why some people are more predisposed than others to certain diseases or why some
people respond better than others to the treatments used against some diseases. You
can also learn what environmental factors influence people with a particular genetic
heritage that make developing certain diseases and those same people in another
environment does not develop.
MEDICAL UTILITY
16. This whole set of genetic
studies / genome will allow
researchers to develop better
treatments for some diseases
and determine which factors
should be avoided by individuals
to prevent the development of
some diseases.
MEDICAL UTILITY
17. We know some genes are involved in certain hereditary
diseases and today are conducting studies to discover exactly
the site or sites in the genome that are involved in it, knowing
the risk factor in the population and its mode of inheritance.
Also used for human identification, for paternity, forensic
identification which has been very useful in the judicial
branch.
MEDICAL UTILITY
18. And as we know the great utility of
the DNA molecule, it is also clear the
importance of today of repair
damage to the genome level. In this
way, doctors can improve and even
slow the lives of their patients.
MEDICAL UTILITY
19. BIBLIOGRAPHY
¨ Science daily , “ 'Cowcatcher' Enzyme Fixes Single-Strand DNA “ ,
published on July 29 2013, in the National Academy of Sciences,
[ http://www.sciencedaily.com/releases/2013/07/130729161751.htm ] .
¨ Science daily, “Key Step in Molecular 'Dance' That Duplicates
DNA Deciphered ” , published on July 14 2013, in the journal Nature
Structural & Molecular Biology , [
http://www.sciencedaily.com/releases/2013/07/130714160514.htm ] .