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1. GENE CLONING
DONE BY
SAMEERASADAF
IInd BSC-BIOCHEMISTRY
ISLAMIAH WOMENS ARTS AND SCIENCE COLLEGE

2. GENE
A Gene is a basic physical and
functional unit of heredity. Genes
which are made up of DNA act as
institutions to molecule called protein

3. CLONING
Cloning is the process of generating an
identical copy of cell or an organism.
Clones are that organism that have
identical gene

4. GENE CLONING
Gene cloning is a genetic engineering
techniques in which a gene of interest
is fused into a plasmid and introduce
into a suitable host,self replicate and
generate a large number of identical
copies of the particular gene

5. STEPS INVOLVED IN GENE CLONING
 Generation of foreign DNA molecule
 Selection suitable vector
 Insertion of foreign DNA into vector
 Recombinant DNA introduce into a host cell
 Selection and screening of recombinant clones

6. ESSENTIAL COMPONENTS FOR CLONING A GENE
 Enzymes of cutting DNA fragments-Restriction
endonuclease
 Enzymes of join DNA fragments-DNA ligase
 Vehicles for introducing the recombinant
molecule into host cell-Vector
 DNA fragments-Gene libraries
 Selection of the transformed cell for the presence
of the rDNA

7. CYCLE OF GENE CLONING

8. APPLICATIONS OF GENE CLONING
 Production of protein from cloned gene
 Production of recombinant pharmaceuticals in
medicine
EG:Recombinant Insulin
 Synthesis of human growth hormones in E.coli
 Recombinant vaccine
EG:Vaccine for hepatitis B
 The gene addition approach to plant genetic
engineering in agricultural field
EG:plants that make their own insecticides
herbicides resistant crops

9.  Identifying the genes in a genomes sequence
determing the function of unknown genes
 Studying protein –protein interactions

10. THANK YOU