Mitochondria are cytoplasmic organelles found in eukaryotic cells that generate most of the cell's supply of adenosine triphosphate (ATP), used as a source of chemical energy. They contain a double membrane, with the inner membrane folded into cristae that contain enzymes involved in oxidative phosphorylation. Mitochondria also contain their own circular DNA and ribosomes. They are believed to have originated from symbiotic bacteria and play a key role in cellular respiration by harnessing energy from the oxidation of carbohydrates and fats to produce ATP.
Presentation on Electrical Properties of Cell MembraneRubinaRoy1
Cell membrane has the characteristic property to receive stimulus and convey the message through electrical signals, itself getting depolarized and repolarized.
“Foundations of Biochemistry” is a process‐oriented guided inquiry learning (POGIL) style workbook for use in upper division Biochemistry courses. The book contains 36 exercises, which could be used for an almost‐exclusively POGIL one semester course or supplemented with lectures, case studies, or student presentations for a full year course. It is intended as a supplement to a textbook, and the very modest price makes it a very cost‐effective educational resource.
This power point presentation consists of 64 slides including information about plant and other type of cell wall. Chemical composition, structure, function and properties of cell wall have been explained. Ultra structure of plant cell wall has also been high lighted. Algal,Fungal,Bacterial and Archaeal cell walls have also been explained.
Mitochondria are membrane-bound cell organelles (mitochondrion, singular), known as the power house of the cell that generate most of the chemical energy needed to power the cell's biochemical reactions. Mitochondria generates most of the cell's supply of adenosine triphosphate (ATP), by a process called
“oxidative phosphorylation”.
Structure and function of plasma membrane 2ICHHA PURAK
The presentation consists of 72 slides,describes following heads
DEFINITION : STRUCTURE OF PLASMA MEMBRANE
COMPONENTS OF PLASMA MEMBRANE ( (BIOCHEMICAL PROPERTIES)
LIPID BILAYER
PROTEINS
CARBOHYDRATES
CHOLESTEROL
MODELS EXPLAINING STRUCTURE OF BIO MEMBRANE
FLUID MOSAIC MODEL
MOBILITY OF MEMBRANE
GLYCOCALYX : GLYCOPROTEINS AND GLYCOLIPIDS
TRANSPORT OF IONS AND MOLECULES ACROSS PLASMA MEMBRANE
FUNCTIONS OF PLASMA MEMBRANE
DIVERSITY OF CELL MEMBRANES
SITE OF ATPASE ION CARRIER CHANNELS AND PUMPS-RECEPTORS
Diversity of cell size & shape By KK Sahu SirKAUSHAL SAHU
SYNOPSIS
Introduction to cell
Historical Aspects
Cell Diversity
Types Of Cell Diversity
Cell Diversity In Origin
Cell Diversity In size
Cell Diversity In Shape
Some Other Types
5) Differentiation And Specialisation Of Cell Diversity
6) Conclusion
7) References
Surrogacy in simple terms can be defined as an Assisted Reproductive Technique (ART) through which a woman carries pregnancy for another person or couple (intending parents or commissioning parents) and such woman is known as surrogate mother.
for more information:
Dr.Samit Sekhar
www.kiranivfgenetic.com
Email:info@kiranivfgenetic.com
Cell : +91-994-817-5768.
Presentation on Electrical Properties of Cell MembraneRubinaRoy1
Cell membrane has the characteristic property to receive stimulus and convey the message through electrical signals, itself getting depolarized and repolarized.
“Foundations of Biochemistry” is a process‐oriented guided inquiry learning (POGIL) style workbook for use in upper division Biochemistry courses. The book contains 36 exercises, which could be used for an almost‐exclusively POGIL one semester course or supplemented with lectures, case studies, or student presentations for a full year course. It is intended as a supplement to a textbook, and the very modest price makes it a very cost‐effective educational resource.
This power point presentation consists of 64 slides including information about plant and other type of cell wall. Chemical composition, structure, function and properties of cell wall have been explained. Ultra structure of plant cell wall has also been high lighted. Algal,Fungal,Bacterial and Archaeal cell walls have also been explained.
Mitochondria are membrane-bound cell organelles (mitochondrion, singular), known as the power house of the cell that generate most of the chemical energy needed to power the cell's biochemical reactions. Mitochondria generates most of the cell's supply of adenosine triphosphate (ATP), by a process called
“oxidative phosphorylation”.
Structure and function of plasma membrane 2ICHHA PURAK
The presentation consists of 72 slides,describes following heads
DEFINITION : STRUCTURE OF PLASMA MEMBRANE
COMPONENTS OF PLASMA MEMBRANE ( (BIOCHEMICAL PROPERTIES)
LIPID BILAYER
PROTEINS
CARBOHYDRATES
CHOLESTEROL
MODELS EXPLAINING STRUCTURE OF BIO MEMBRANE
FLUID MOSAIC MODEL
MOBILITY OF MEMBRANE
GLYCOCALYX : GLYCOPROTEINS AND GLYCOLIPIDS
TRANSPORT OF IONS AND MOLECULES ACROSS PLASMA MEMBRANE
FUNCTIONS OF PLASMA MEMBRANE
DIVERSITY OF CELL MEMBRANES
SITE OF ATPASE ION CARRIER CHANNELS AND PUMPS-RECEPTORS
Diversity of cell size & shape By KK Sahu SirKAUSHAL SAHU
SYNOPSIS
Introduction to cell
Historical Aspects
Cell Diversity
Types Of Cell Diversity
Cell Diversity In Origin
Cell Diversity In size
Cell Diversity In Shape
Some Other Types
5) Differentiation And Specialisation Of Cell Diversity
6) Conclusion
7) References
Surrogacy in simple terms can be defined as an Assisted Reproductive Technique (ART) through which a woman carries pregnancy for another person or couple (intending parents or commissioning parents) and such woman is known as surrogate mother.
for more information:
Dr.Samit Sekhar
www.kiranivfgenetic.com
Email:info@kiranivfgenetic.com
Cell : +91-994-817-5768.
About how cellular respiration occurs in Mitochondria, it discusses first the parts and functions of mitochondrion then the types of respiration and the 3 processes occurs in aerobic respiration.
Dr.S.KARTHIKUMAR
Associate Professor
Department of Biotechnology
Kamaraj College of Engineering and Technology, K.Vellakulam-625701, TN, India
Email: skarthikumar@gmail.com
Biochemistry serves as a fundamental discipline in the life sciences, exploring the chemical processes and biomolecules that underlie biological systems. It bridges the gap between biology and chemistry, investigating the molecular basis of life. Biochemistry delves into the study of macromolecules such as proteins, nucleic acids, carbohydrates, and lipids, as well as the intricate interactions and reactions that occur within cells. It encompasses vital topics such as metabolism, energy production, cellular respiration, and photosynthesis. The field examines DNA, RNA, and gene expression to unravel the genetic information and molecular mechanisms that govern living organisms. Additionally, biochemistry explores the molecular structures, chemical bonds, and synthesis of biomolecules, as well as the diverse biochemical pathways and cellular functions they regulate. It also encompasses aspects of molecular genetics, protein synthesis, enzyme kinetics, biochemical regulation, and cell signaling. Biochemistry finds applications in various areas including biotechnology, pharmaceuticals, genetic engineering, and the study of metabolic diseases. It plays a pivotal role in advancing our understanding of life at the molecular level and holds significant implications for numerous scientific and medical advancements.
CELL STRUCTURE, CELL ORGANELLES, CELL FUNCTIONS.
BRIEF IDEA ABOUT CELL STRUCTURE, CELL ORGANELLES AND THEIR FUNCTIONS, COMPARTMENTALIZATION INSIDE CELL
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
2. Introduction
• The mitochondria (Gr., mito=thread,chondrion=granule) are granular
cytoplasmic organelle
• Are present in all aerobic cells of higher animals and plants and certain
micro-organisms including algae, protozoa and fungi.
• They are absent in bacterial cells
• Vital stain Janus green stains living mitochondria greenish blue due to its
action with cytochrome oxidase
3. Historical
• The mitochondria were first observed by Kolliker in 1850
• And term coined by Benda (1897-1897)
• Altman suggested name bioblast for the mitochondria
4. Distribution or localization
• The distribution and number of mitochondria is depend on functional state
of that cell
• Typically mitochondria with many cristae are associated with mechanical
and osmotic work situations
• More demand of ATP more number of mitochondria. E.g. muscle cells
• Myocardial muscle cells have numerous large mitochondria called
sacrosomes.
• The cells of green plants contain less number of mitochondria than animals
because in plant cells the function of mitochondria is taken over by
chloroplast
5. Biogenesis
• Some theories suggests that mitochondria are originated de novo from the
simple building blocks like amino acids and lipids but there is not direct
evidence
• Fission of mitochondria leads to formation of two new mitochondria
7. Structure
a) Membrane - Mitochondria is bonded by double membrane. The outer
membrane contains many copies of transport protein called porin. Outer
membrane is permeable to all molecules. Inner membrane is impermeable
forming a series of infoldings known as cristae.
b) Oxysomes - also known as inner membrane subunits, elementary particles
F1 particles or f0-F1 particles and are meant for ATP synthesis
(phosphorylation) and also for ATP oxidation (acting as ATP synthetase
and ATPase)
c) Matrix- mitochondria matrix is homogeneous, gel-like proteinaceous
material. Matrix contains lipids, proteins, circular DNA, ribosomes and
granules
8. Chemical composition
• Mitochondria are found to contain 65-70% protein, 25-30% lipids,0.5%
RNA and small amount of DNA.
• Lipid contents of mitochondria are composed 90% phospholipids.
• The inner membrane is rich in cardiolipin (type of phospholipid) which
makes this membrane impermeable
• Mitochondria contains variety of ions like Na, K, Cl, AMP, and so on
9. Chemical composition-Enzymes
I. Enzyme of outer membrane- important enzymes of outer membrane are
monoamine oxidase, NADH-cytochrome C-reductase.
II. Enzymes of intermembrane space- adenylate kinase an nucleotide
diphosphokinase
III. Enzymes of inner membrane- ATP synthetase, cytochrome oxidase and
succinate dehydrogenase
IV. Enzymes of matrix -matrix contains hundreds of enzymes including those
required for oxidation of pyruvate and fatty acids and for citric acid cycle
or Krebs cycle. Contains enzymes like malate dehydrogenase, isocitrate
dehydrogenase, citrate synthetase and so on
10. Function- ATP synthesis
• What is ATP?
• ATP- The adenine and ribose sugar collectively constitute the nucleoside
adenosine, which by having one, two or three phosphate groups forms
AMP, ADP or ATP.
• In ATP last phosphate group is linked with ADP by a special bond known
as energy rich bond. Breakage of this bond releases energy
A P-P-P = A P-P + Pi + 7300 calories
11. Function- ATP synthesis
• Oxidation of carbohydrates
a) Glycolysis- under anaerobic conditions glucose is degraded into lactic acid
by a process of glycolysis (generates 2 ATP)
b) Krebs cycle and ETS- two molecules of FADH2 and 6 molecules of NADH
produced in Krebs cycle (from 2 molecules of acetyl CoA) are oxidised by
molecular o2 in ETS and produces ATP molecules
• Oxidation of fat- during β-oxidation of two molecules of ATP are releases
and total 5 molecules of ATP are produced.