This document discusses the mechanism of enzyme action through a learning outcome and several sections. It begins with introducing the topic of enzyme mechanisms and defining enzymes as biological catalysts for cellular reactions. It then explains the "lock and key" hypothesis for enzyme action, where a specific substrate molecule binds to the active site of an enzyme, forming an unstable enzyme-substrate complex. This allows the substrate to be converted into products, which then leave the active site so the enzyme can catalyze another reaction. The document assessments students' understanding with multiple choice and short answer questions about enzyme specificity and how temperature affects reaction rates.

1. EDT 5303
TECHNOLOGY AND
INNOVATION IN EDUCATION
( GROUP 12)
TOPIC :
MECHANISM OF
ENZYME ACTION
1 . N U R F AU ZAN A BT ZU LKIF LI (G 1 027668)
2. N O R ‘AID AH BT LU K M AN (G
1 02731 2)
3. WAN S U H AILA BT WAN IS M AIL (G 1 022282 )

2. C O N TE N T
LEARNING
1 OUTCOME
2 INTRODUCTION
PRIOR
3 KNOWLEDGE
DEFINITION OF
4 ENZYME
MECHANISM OF
5 ENZYME
ACTION
6 ASSESSMENT

3. LE AR N IN G O U TC O M E
4.10 :
Explain the
mechanism of
enzyme
action

4. IN TR O D U C TIO N
Do you realize the
term of COENZYME
on detergent
products?
CLICK HERE !!

5. P R IO R K N O WLE D G E
RECALL !!!
Maltose + Water Glucose + Glucose
ENZYME maltase BREAK
DOWN maltose into 2
molecules of glucose

6. P rior knowle d ge
Enzyme
s
Cloth
Stains
How the stains have been
removed from the cloth?

7. D E F IN ITIO N O F E N ZYM E
Enzymes are biological
catalyst that direct or guide
almost all cellular reactions.

8. M E C H AN IS M O F E N ZM E
AC TIO N
‘LOCK AND KEY’
HYPOTHESIS

9. M E C H AN IS M O F E N ZM E
AC TIO N
Product
Product
Enzyme Enzyme-substrate complex
EXPLANATION EXPLANATION EXPLANATION
Substrate

10. M E C H AN IS M O F E N ZM E
AC TIO N
A specific substrate molecule
arrives at the active site of the
enzyme molecule.

11. M E C H AN IS M O F E N ZM E
AC TIO N
The substrate molecule binds to
the active site to form an
enzyme-substrate complex
which is very unstable.

12. M E C H AN IS M O F E N ZM E
AC TIO N
Product molecules leave the
active site of the enzyme.
The enzyme is free to take
another substrate molecule
into its active site to catalyse
another reaction.

13. M E C H AN IS M O F E N ZM E
AC TIO N
Which substrate can bind to active site of enzyme sucrase?
Sucrose can bind to
active site of sucrase
Enzymes
have
specific
sites called
Sucrose
active site
which bind
to specific
substrates
Sucrase Maltose
Maltose CANNOT bind to
active site of sucrase

14. as s e s s m e nt
Question 1 Question 2
Question 3 Question 4

15. Q u e s tion 1
Fill the blank with the correct answer.
2. A specific ______________ molecule arrives Product
at the active site of the ______________ molecule.
enzyme-
substrate
2. The substrate molecule binds to the active site of the complex
enzyme to form an ______________.
enzyme
3. The enzyme is free to take another substrate molecule
active site
into its ______________ to catalyse another reaction.
substrate
4. ______________ molecules leave the active site of the
enzyme.

16. Q u e s tion 2
Which of the following shows the characteristic of enzymes?
A. Enzyme reaction is specific
A. Enzyme reaction is influenced by pH
C. Enzyme reaction is influence by temperature
D. The structure of the enzyme change at the end
of the reaction.

17. Q u e s tion 3
Protease Dissolve starch
stains on cloth
Amylase Ripening cheese
Lipase Dissolve protein
stains on cloth

18. Q u e s tion 4
Enzyme is used to wash away the blood
stain on a shirt.
Explain how the enzyme acts on the
blood stain at a maximum rate.
(3 MARKS)
ANSWER :
Blood is made up from proteins. Protease in detergent
will break down protein into amino acids. This reaction
occur effectively at 40 ºC which is optimum temperature
for enzyme action.

19. THANK YOU ….