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MarineBiotechnology




1               11/17/2011
ALGAE

    By: Ayesha Nawaz
        Daniela Lopez




2    11/17/2011
What is it?

       Marine biotechnology uses biological material
        from the sea to produce goods and services.




    3                                   11/17/2011
Areas Of Interest
       Extraction of biologically-active compounds or
        pharmaceuticals
       Cloning of proteins of marine origin
       Analysis of marine toxins and anti-venoms
       Development of industrial adhesives
       Development of diagnostic probes for marine
        pathogens.
       Bio-remediation, which uses marine and other
        organisms to digest contaminants and toxins in
        the environment.


    4                                    11/17/2011
Constraints
   Like all other technologies the opportunities
    involve long lead times and high risks.
   Other critical issues that can affect bio-
    prospecting for genetic material include access
    and ownership of intellectual property rights.
   Ability to sustainably produce high-oil-yielding
    algae strains on a large-scale.
   Ability to extract the oil from the algae on a
    large scale.
   Capability for large-scale conversion of algal oil
    into                                    biodiesel.


    5                                    11/17/2011
Algal Strains
       Some prominent strains of algae that have a
        high carbohydrate content and hence are
        promising candidates for ethanol production.

           Sargassum
           Glacilaria
           Prymnesium parvum
           Euglena gracilis




    6                                   11/17/2011
Ethanol from Algae
       Algae have a tendency to have a much different
        makeup than does most feed stocks used in
        ethanol, such as corn and sugar cane.
        Ethanol from algae is possible by converting the
        starch (the storage component) and Cellulose (the
        cell wall component). lipids in algae oil can be
        made into biodiesel, while the carbohydrates can
        be converted to ethanol.
       Algae are the optimal source for second generation
        bioethanol due to the fact that they are high in
        carbohydrates/polysaccharides and thin cellulose
                               walls



    7                                       11/17/2011
Process behind Ethanol from Algae
      Fermentation process to produce ethanol include the following
      stages:


     Growing starch-
accumulating, filament-                                         Separating the
                              Harvesting the grown
   forming, or colony-                                      resulting ethanol from
                                algae to form a
  forming algae in an                                          the fermentation
      aqua culture                 biomass;
                                                                    solution.
      environment;




                                 Contacting the
                               decaying biomass
                              with a yeast capable         Initiating decay of the
                               of fermenting it to                  biomass;
                              form a fermentation
                                     solution;
     8                                                  11/17/2011
Decaying…
    Cellular structure of the biomass begins to decay
    (e.g., cell wall rupture) and release the
    carbohydrates.
    Initiating decay can be accomplished
    mechanically, non-mechanically.
   The yeasts used are typically brewers‟ yeasts
    (Saccharomyces cerevisiae and Saccharomyces
    uvarum).
   Genetically altered bacteria could be useful for
    fermentation can also be used.



    9                                   11/17/2011
Ethanol from De-oiled Algae Biomass



Algal Biomass     Left-over           Conversion into
• Carbohydrates   mass(once the       Ethanol
• Proteins        lipids extracted)   • Carbohydrates in
• Lipids          • Carbohydrates       the left-over algae
                  • Proteins            can be converted
                                        into sugars.
                                      • Sugars can be
                                        processed into
                                        Ethanol




 10                                     11/17/2011
Production Of Ethanol and Biodiesel from
Algae




     FromOligae, 2006, Retrievedfrom http://www.oilgae.com/algae/pro/eth/eth.html



11                                                                                  11/17/2011
Biodiesel from Algae


                                                               Dewatering             Aquafeed
                    Furthertreatm                                                    Animal feed
                    enttorecover
                                                              and Extrusion
                                                                                       Petfeed
                        diesel
                                               Residual
                                              microalgae
                                                                                 Incorporatedi
Selectionofmi                                                 Extraction of
                                                                                   nto human
croalgaespec        Wasteliquor                                  protein
                                                                                      food
     ies



 Growth of                          Extraction of      Oil for
                Harvesting of
 microalgae                         oilfrommicroa   processingint        Biodiesel
                 microalgae
                                          lgae        oBiofuel




    12                                                              11/17/2011
Advantages to make ethanol from algae
instead of diesel

   The lipid (oil) content in algae from different sources
    max. 70% is less than starch+ cellulose+ sugars nearly
    100% content.
   Algae should be dried (a lot of energy) to extract oil
    but needs no treatment for ethanol fermentation.
   Extracting the oil from algae is complicated.
   CO2 from ethanol fermenting can be used as algae
    feedstock.
   The energy from fermenting and distilling can be
    used to heat algae ponds (photo bioreactors) in
    cold climate.

    13                                      11/17/2011
Advantages of Using Algae

    Algae have many important advantages over other
     oil-producing crops, like corn, canola and soybeans.
     It can be grown in almost any enclosed space and
     it multiplies rapidly and requires very few inputs to
     flourish - mainly just sunlight, water and carbon
     dioxide.
    Because algae has a high surface-area-to-volume
     ratio, it can absorb nutrients very quickly, and its
     small size is what makes it mighty.
    The Energy Returned is much higher than Energy
     Invested or required to produce algae ethanol.

    14                                     11/17/2011
Advantages of Using Algae
    Algae Consume CO2, a Major Greenhouse Gas.
    Do Not Require Arable Land.
    Grow Very Rapidly.
    Represent a “New” Source of Fuel.
    Represent a New Source of Animal Food.




    15                              11/17/2011
Simpler…
    Algae ethanol does not require a very
     complicated equipment or machinery to set it
     up
    As Scientists and researchers of Canadian
     National Renewable Energy Association have
     observed that: "algae ethanol plant does not
     eat up the country's bread basket" and gives to
     mankind many valuable bi-products that are
     used in several ways.




    16                                 11/17/2011
•Large scale cultures to fulfil
                         demands
             Scale      •Ponds vs.
                         photobioreactors*




                               •Large area requirements
                               •Good climatic conditions
Challenges       Land          •Close to resources (water,
                                CO2)
                               •Flat land




                        •Large amount of water for
                         culture
                        •Saline water and
             Water       evaporation replacement
                        •Complete discharge of
                         ponds due to increased
                         salinity
17                                   11/17/2011
•Phosphate. Non-renewable and
                           competition with food crops
                          •Nitrogen.
                           •6-8% of dry microalgae.
                           •Can affect lipid composition and
             Nutrients      culture
                           •2 kg of CO2 kg-1 of N
                           •Waste water?. Inconsistent
                            composition
                          •CO2. Transportation costs




                                  •20-30% of the total cost

Challenges       Harvesting       •Different sizes and shapes (2 to 200
                                   m for individual cells
                                  •Keeping the biomass newtonian




                           •Homogenization & bead milling
                Cell       •Cooling systems due to energy
             disruption    dissipation
                           •Reducing cell wall strength


18                                       11/17/2011
Open orclosed?

                                      Ponds                    PBR
                                                                     Expensive
                                                                     construction
                                              Low cost
                                                                     and operative
                                                                     costs


                                                                     Monitoring
                                                                     systems eg. Gas
                                              Simplicity
                                                                     exchanging
                                                                     system



                                              High
                                                                     Easier water
                                              evaporation
                                                                     recycling
                                              rates
From “
Placingmicroalgaeonthebiofuelspri
oritylist: a
reviewofthetechnologicalchalleng
                                              Culture
es”, by Greenwellet al, 2009, J. R.                                  Controlled        Bios (2008).
Soc. Interface, 7, p 708                      contamination,                           PhotoBioReactorSculpture.
                                                                     conditions
                                              temperature.                             RetrievedOct 7 2011 from
                                                                                       http://biosarch.wordpress.com/200
                                                                                       8/07/08/photobioreactor-
                                                                                       sculpture/



        19                                                                   11/17/2011
Algae vs. crops




                                                            From “Biotech‟sgreen gold?”, by Waltz E. 2009, NatureBiotechnology, 27, p. 16


RetrievedOct 7 2011
fromhttp://www.odec.ca/projects/2008/adit8i2/benefit.html




  20                                                                                           11/17/2011
Economicalevaluation

Base case (current technology)        Projected case
                                      (achievablebutnotdemonstrated)
Productionofbiomassusing500 ha
systems                               =
Oil extraction                        =
Co-                                   Notconsidered(Smallmarkets)
productionofhighvalueproduct(HV
P)


      Internalrateofreturn(IRR). Valuesabove15% are consideredprofitable




 21                                                   11/17/2011
Economicalevaluation




22                                                                                                               11/17/2011
     From “Aneconomicandtechnicalevaluationofmicroalgalbiofuels”, by Stephens E. et al 2010, NatureBiotechnology, 28, p. 127
Economicalevaluation




23                                                                                                               11/17/2011
     From “Aneconomicandtechnicalevaluationofmicroalgalbiofuels”, by Stephens E. et al 2010, NatureBiotechnology, 28, p. 127
Isit profitable?
    Yes, with increasedproductivity/largeproduction
    Estimate: Currenttechnology could produce
     $84/bblbut in thefuture a price of $50/bbl could
     be achieved
    Sinergy with other industries for a
     sustainablesystem




    24                                  11/17/2011
What’s happening?
      Companies         Organizations       Universities

• Aurora biofuels      • Algal biomass   • Algael biofuel
• Algenol                organization      challenge (UK
• Sapphire             • National          academic
  energy                 Algae             institutions)
• Solarvest              Association     • Murdoch +
  BioEnergy            • European          University of
• Solazyme               Algae Biomass     Adelaide
• Chevron [(National     Association     • USA…
  Renewable Energy
  Laboratory (NREL)]   • International
                         Fossil Algae
                         Association


 25                                       11/17/2011
References
    Borowitzka    MA    and   Reza     Navid,         2010,     „Sustainablebiofuelsfromalgae‟,
     MitigAdaptStrategGlobChange, Springer.
    Greenwell HC, Laurens LML, Shields RJ,              Lovitt RW and Flynn KJ, 2010,
     „Placingmicroalgaeonthebiofuelsprioritylist: a reviewofthetechnologicalchallenges‟, J.R.
     Soc.Interface, 7, 703-726
    Gold rushforalgae, (2009, September 24), NatureNews, 461, 460-61
    Oligae. http://www.oilgae.com/
    Sheehan J, Dunahay T, Benemann J andRoessler P (1998), „A Look Back at the U.S.
     DepartmentofEnergy‟sAquaticSpeciesProgram— Biodiesel fromAlgae‟, (Close out report
     1978-1996),   Colorado        USA,      NationalRenewableEnergyLaboratory,    U.S.
     DepartmentofEnergy‟s Office ofFuelsDevelopment
    Stephens E, Ross IL, King Z, Mussgnung JH, Kruse O, Posten C, Borowitzka MA andHankamer
     B, 2010, „Aneconomicandtechnicalevaluationofmicroalgalbiofuels‟, Nat Biotechnol, 28
     (2), 126-128
    Waltz E, 2009, „Biotechs‟sgreen gold?, Nat Biotechnol, 27(1), 15-18




    26                                                                     11/17/2011
THANKS




27            11/17/2011

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Marine Biotechnology/Algae

  • 2. ALGAE By: Ayesha Nawaz Daniela Lopez 2 11/17/2011
  • 3. What is it?  Marine biotechnology uses biological material from the sea to produce goods and services. 3 11/17/2011
  • 4. Areas Of Interest  Extraction of biologically-active compounds or pharmaceuticals  Cloning of proteins of marine origin  Analysis of marine toxins and anti-venoms  Development of industrial adhesives  Development of diagnostic probes for marine pathogens.  Bio-remediation, which uses marine and other organisms to digest contaminants and toxins in the environment. 4 11/17/2011
  • 5. Constraints  Like all other technologies the opportunities involve long lead times and high risks.  Other critical issues that can affect bio- prospecting for genetic material include access and ownership of intellectual property rights.  Ability to sustainably produce high-oil-yielding algae strains on a large-scale.  Ability to extract the oil from the algae on a large scale.  Capability for large-scale conversion of algal oil into biodiesel. 5 11/17/2011
  • 6. Algal Strains  Some prominent strains of algae that have a high carbohydrate content and hence are promising candidates for ethanol production.  Sargassum  Glacilaria  Prymnesium parvum  Euglena gracilis 6 11/17/2011
  • 7. Ethanol from Algae  Algae have a tendency to have a much different makeup than does most feed stocks used in ethanol, such as corn and sugar cane.  Ethanol from algae is possible by converting the starch (the storage component) and Cellulose (the cell wall component). lipids in algae oil can be made into biodiesel, while the carbohydrates can be converted to ethanol.  Algae are the optimal source for second generation bioethanol due to the fact that they are high in carbohydrates/polysaccharides and thin cellulose walls 7 11/17/2011
  • 8. Process behind Ethanol from Algae Fermentation process to produce ethanol include the following stages: Growing starch- accumulating, filament- Separating the Harvesting the grown forming, or colony- resulting ethanol from algae to form a forming algae in an the fermentation aqua culture biomass; solution. environment; Contacting the decaying biomass with a yeast capable Initiating decay of the of fermenting it to biomass; form a fermentation solution; 8 11/17/2011
  • 9. Decaying…  Cellular structure of the biomass begins to decay (e.g., cell wall rupture) and release the carbohydrates.  Initiating decay can be accomplished mechanically, non-mechanically.  The yeasts used are typically brewers‟ yeasts (Saccharomyces cerevisiae and Saccharomyces uvarum).  Genetically altered bacteria could be useful for fermentation can also be used. 9 11/17/2011
  • 10. Ethanol from De-oiled Algae Biomass Algal Biomass Left-over Conversion into • Carbohydrates mass(once the Ethanol • Proteins lipids extracted) • Carbohydrates in • Lipids • Carbohydrates the left-over algae • Proteins can be converted into sugars. • Sugars can be processed into Ethanol 10 11/17/2011
  • 11. Production Of Ethanol and Biodiesel from Algae FromOligae, 2006, Retrievedfrom http://www.oilgae.com/algae/pro/eth/eth.html 11 11/17/2011
  • 12. Biodiesel from Algae Dewatering Aquafeed Furthertreatm Animal feed enttorecover and Extrusion Petfeed diesel Residual microalgae Incorporatedi Selectionofmi Extraction of nto human croalgaespec Wasteliquor protein food ies Growth of Extraction of Oil for Harvesting of microalgae oilfrommicroa processingint Biodiesel microalgae lgae oBiofuel 12 11/17/2011
  • 13. Advantages to make ethanol from algae instead of diesel  The lipid (oil) content in algae from different sources max. 70% is less than starch+ cellulose+ sugars nearly 100% content.  Algae should be dried (a lot of energy) to extract oil but needs no treatment for ethanol fermentation.  Extracting the oil from algae is complicated.  CO2 from ethanol fermenting can be used as algae feedstock.  The energy from fermenting and distilling can be used to heat algae ponds (photo bioreactors) in cold climate. 13 11/17/2011
  • 14. Advantages of Using Algae  Algae have many important advantages over other oil-producing crops, like corn, canola and soybeans.  It can be grown in almost any enclosed space and it multiplies rapidly and requires very few inputs to flourish - mainly just sunlight, water and carbon dioxide.  Because algae has a high surface-area-to-volume ratio, it can absorb nutrients very quickly, and its small size is what makes it mighty.  The Energy Returned is much higher than Energy Invested or required to produce algae ethanol. 14 11/17/2011
  • 15. Advantages of Using Algae  Algae Consume CO2, a Major Greenhouse Gas.  Do Not Require Arable Land.  Grow Very Rapidly.  Represent a “New” Source of Fuel.  Represent a New Source of Animal Food. 15 11/17/2011
  • 16. Simpler…  Algae ethanol does not require a very complicated equipment or machinery to set it up  As Scientists and researchers of Canadian National Renewable Energy Association have observed that: "algae ethanol plant does not eat up the country's bread basket" and gives to mankind many valuable bi-products that are used in several ways. 16 11/17/2011
  • 17. •Large scale cultures to fulfil demands Scale •Ponds vs. photobioreactors* •Large area requirements •Good climatic conditions Challenges Land •Close to resources (water, CO2) •Flat land •Large amount of water for culture •Saline water and Water evaporation replacement •Complete discharge of ponds due to increased salinity 17 11/17/2011
  • 18. •Phosphate. Non-renewable and competition with food crops •Nitrogen. •6-8% of dry microalgae. •Can affect lipid composition and Nutrients culture •2 kg of CO2 kg-1 of N •Waste water?. Inconsistent composition •CO2. Transportation costs •20-30% of the total cost Challenges Harvesting •Different sizes and shapes (2 to 200 m for individual cells •Keeping the biomass newtonian •Homogenization & bead milling Cell •Cooling systems due to energy disruption dissipation •Reducing cell wall strength 18 11/17/2011
  • 19. Open orclosed? Ponds PBR Expensive construction Low cost and operative costs Monitoring systems eg. Gas Simplicity exchanging system High Easier water evaporation recycling rates From “ Placingmicroalgaeonthebiofuelspri oritylist: a reviewofthetechnologicalchalleng Culture es”, by Greenwellet al, 2009, J. R. Controlled Bios (2008). Soc. Interface, 7, p 708 contamination, PhotoBioReactorSculpture. conditions temperature. RetrievedOct 7 2011 from http://biosarch.wordpress.com/200 8/07/08/photobioreactor- sculpture/ 19 11/17/2011
  • 20. Algae vs. crops From “Biotech‟sgreen gold?”, by Waltz E. 2009, NatureBiotechnology, 27, p. 16 RetrievedOct 7 2011 fromhttp://www.odec.ca/projects/2008/adit8i2/benefit.html 20 11/17/2011
  • 21. Economicalevaluation Base case (current technology) Projected case (achievablebutnotdemonstrated) Productionofbiomassusing500 ha systems = Oil extraction = Co- Notconsidered(Smallmarkets) productionofhighvalueproduct(HV P) Internalrateofreturn(IRR). Valuesabove15% are consideredprofitable 21 11/17/2011
  • 22. Economicalevaluation 22 11/17/2011 From “Aneconomicandtechnicalevaluationofmicroalgalbiofuels”, by Stephens E. et al 2010, NatureBiotechnology, 28, p. 127
  • 23. Economicalevaluation 23 11/17/2011 From “Aneconomicandtechnicalevaluationofmicroalgalbiofuels”, by Stephens E. et al 2010, NatureBiotechnology, 28, p. 127
  • 24. Isit profitable?  Yes, with increasedproductivity/largeproduction  Estimate: Currenttechnology could produce $84/bblbut in thefuture a price of $50/bbl could be achieved  Sinergy with other industries for a sustainablesystem 24 11/17/2011
  • 25. What’s happening? Companies Organizations Universities • Aurora biofuels • Algal biomass • Algael biofuel • Algenol organization challenge (UK • Sapphire • National academic energy Algae institutions) • Solarvest Association • Murdoch + BioEnergy • European University of • Solazyme Algae Biomass Adelaide • Chevron [(National Association • USA… Renewable Energy Laboratory (NREL)] • International Fossil Algae Association 25 11/17/2011
  • 26. References  Borowitzka MA and Reza Navid, 2010, „Sustainablebiofuelsfromalgae‟, MitigAdaptStrategGlobChange, Springer.  Greenwell HC, Laurens LML, Shields RJ, Lovitt RW and Flynn KJ, 2010, „Placingmicroalgaeonthebiofuelsprioritylist: a reviewofthetechnologicalchallenges‟, J.R. Soc.Interface, 7, 703-726  Gold rushforalgae, (2009, September 24), NatureNews, 461, 460-61  Oligae. http://www.oilgae.com/  Sheehan J, Dunahay T, Benemann J andRoessler P (1998), „A Look Back at the U.S. DepartmentofEnergy‟sAquaticSpeciesProgram— Biodiesel fromAlgae‟, (Close out report 1978-1996), Colorado USA, NationalRenewableEnergyLaboratory, U.S. DepartmentofEnergy‟s Office ofFuelsDevelopment  Stephens E, Ross IL, King Z, Mussgnung JH, Kruse O, Posten C, Borowitzka MA andHankamer B, 2010, „Aneconomicandtechnicalevaluationofmicroalgalbiofuels‟, Nat Biotechnol, 28 (2), 126-128  Waltz E, 2009, „Biotechs‟sgreen gold?, Nat Biotechnol, 27(1), 15-18 26 11/17/2011
  • 27. THANKS 27 11/17/2011