Biotechnology being multidisciplinary subject has applications in different areas. Marine Biotechnology is the field dealing with the uses of marine organisms for human use.
The drugs which are obtained from marine organisms are know as marine drugs. these marine drugs are used since ancient times. chines and japanes are very famous to use these resources. And interstingly,innumarable products derived from the marine organisms in several 'crude forms' have been widely used across the globe by the traditional practitioners for thousands of years.
Cancer is one of the fatal disease that has no known complete cure.One of approach in developing the novel drug is through unraveling the enigma of marine biodiversity.
Biotechnology being multidisciplinary subject has applications in different areas. Marine Biotechnology is the field dealing with the uses of marine organisms for human use.
The drugs which are obtained from marine organisms are know as marine drugs. these marine drugs are used since ancient times. chines and japanes are very famous to use these resources. And interstingly,innumarable products derived from the marine organisms in several 'crude forms' have been widely used across the globe by the traditional practitioners for thousands of years.
Cancer is one of the fatal disease that has no known complete cure.One of approach in developing the novel drug is through unraveling the enigma of marine biodiversity.
Polyketide Synthase type III Isolated from Uncultured Deep-Sea Proteobacteriu...Hadeel El Bardisy
Screening and Isolation of possible bacterial PKS type III in Atlantis II deep brine pool using a metagenomic approach
and gaining a deeper insights into the evolutionary origin of PKS type III among Prokaryotes and Eukaryotes
Jamur yang diklaim menunjukkan antitumor, antivirus, antibiotik, anti-inflamasi, hipoglikemik, kegiatan hipokolesterolemik dan hipotensi, serta memiliki nilai terapeutik dan gizi (Chang dan Miles, 1989; Breene, 1990; Miles dan Chang, 1997). Aktivitas antitumor adalah medisinal efek yang paling diteliti secara menyeluruh terutama dari jamur shiitake, jamur maitake, Sclerotina sclerotiorum dan HHB (Borchers, 1999).
Aktivitas antibakteri ekstrak metanol dari tiga Polypore jamur Phellinus rimosus, Ganoderma lucidum dan Navesporus floccosa dipelajari (Sheena et al., 2003). Studi pada sifat antimikroba dari Ganoderma lucidum, Fomes lignosus, HHB, Pleurotus florida, Lentinus subnudus, Leptoporus sp, Panus fulvus, Coriolus versicolor, Trametes saepiara, Trametes betulina, Daedalea elegans dan Auricularia auricula dilakukan (Fagedev, Oyalede, (2009).
Ekstrakdari etil asetat, kloroform, aseton dan etil alkohol Pleurotus ostreatus (Jack ex Fr.) Kum var. salignus, Pleurotus florida Fovose, HHB Fr., Helvella leucomelaena (Pexs) Nannf. dan Amanita virosa (Fr.) Bertillon diselidiki Kegiatan antimikrobanya terhadap Escherichia coli ATCC 25922, Staphylococcus aureus dan Pseudomonas aureginosa ATCC 27857 dengan Metode Difusi (Demirhan et al., 2007). Dalam penelitian ini, kegiatan antijamur Pseudoclitocybe cyathiformis Bull.
Ekstraksi dengan bantuan aseton, kloroform terhadap ke Fusarium moniliforme dan Fusarium culmorum diselidiki.
Balai Perikanan Budidaya Laut Batam
Alternative strategies for minimizing the detrimental effects of bacterial infection and prevention of diseases in aquaculture are necessary since the ongoing efficacy of antibiotics is proving to be unsustainable. One of the most promising approach is the use of aqua herbal conditioners to stimulate the immune system of fish to allow them to fight off infections. In this study, the protective effect of aqua herbal conditioners produced from, mainly, mangrove and neem plant extracts in marine fish, was tested on Asian Seabass Lates calcarifer and Silver Pompano Trachinotus blochii at 8-10 g of weight size. Challenge tests were performed by immersion with two pathogenic bacteria: Vibrio harveyi and Vibrio parahaemolyticus, at a concentration of 105 cells ml-1 for 60 minutes after 12 h, 24 h and 36 h conditioning treatment. The experimental trial show that after 72 h, commercially available aqua herbal conditioners (AquaHerb) was able to significantly increase the percentage survival of L. calcarifer and T. blochii and reduces their susceptibilityto the V.harveyi and V.parahaemolyticus. Significantly higher leukocytesnumber, monocyte, neutrophil andphagocyticindexwere detected in all conditioning group for Silver Pompano and Asian Seabass. These results suggest that the combination of herbal extracts together with other trace elements contained in AquaHerb were able to act as immunostimulants and appear to improve the immune status and disease resistance of Asian Seabass and Silver Pompano.
Isolation of Bioactive Secondary Metabolites from Seaweeds Amphiroa anceps ag...Ferdinand .S. Lubobi
The biodiversity of ocean living resources provide an ample scope for the extraction of drugs and chemicals for therapeutic purposes. Sea weed Amphiroa anceps, collected from Thondi-Palk Strait region of Tamil Nadu, was studied for antagonistic activity of proteins. Seaweed species were identified based on specula morphology. Ethyl
Acetate extracts yielded a total of 0.9 g, 0.12 g, 0.02 g, 0.14 g and 0.17 g from 1.5 g of sponge respectively. The antagonistic activity of crude extract against bacterial pathogens showed clear inhibition zones against Yersinia sp., Streptococcus sp. and Vibrio sp. The extracted Seaweed strain had Minimum Inhibitory Concentrations against all the three chicken meat associated pathogens, Vibrio sp., Yersinia sp. and Streptococcus sp. and Minimum Bactericidal Concentration against two chicken meat associated pathogens, Vibrio sp. and Yersinia sp.
A Review on the Antimicrobial Activity of Sesuvium Portulacastrumijtsrd
Mangroves are unique group of vascular plants that occur in saline coastal habitats and are known to tolerate extreme environmental conditions. Some mangrove plants are used for a wide range of conditions, including bacterial, fungal, and viral diseases. The rise of antibiotic-resistant microorganisms is one of the severe problems in healthcare systems of the world, and infectious diseases are the second most serious cause of death worldwide. Therefore, new drugs have to be found in order to combat such diseases and it is essential to find new compounds that have antimicrobial properties. Medicinal-plant extracts, known to produce certain bioactive molecules which react with other organisms in the environment, are known to be less toxic to humans and are environmentally friendly due to the less pollutant released during production. Antimicrobial properties of medicinal plants are being increasingly reported worldwide. Mangroves are biochemically unique and produce a wide array of novel natural products and are considered a rich source of steroids, triterpenes, saponins, flavonoids, alkaloids, and tannins. Extracts from the leaves, stems, barks, and roots of mangrove species have shown positive results for antioxidant activity tests. G. Beulah | D. Divya"A Review on the Antimicrobial Activity of Sesuvium Portulacastrum" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-4 , June 2018, URL: http://www.ijtsrd.com/papers/ijtsrd14540.pdf http://www.ijtsrd.com/biological-science/zoology/14540/a-review-on-the-antimicrobial-activity-of-sesuvium-portulacastrum/g-beulah
Unveiling the Ocean's Pharmacy: A Deep Dive into Drugs from Marine Organisms ...The Lifesciences Magazine
In this article, we delve into the fascinating realm of "drugs from marine organisms," exploring the potential, challenges, and groundbreaking research in this exciting field.
Hepatoprotective and stress - reducing effects of dietary Moringaoleifera ext...Agriculture Journal IJOEAR
Abstract— The main aim of the present study was to evaluate the hepatoprotective and stress-reducing effects of Moringaoleifera extract against A. hydrophila infection and transportation- induced stress in Nile tilapia, Oreochromisniloticus fingerlings. Fish were fed diets representing different supplementation levels of Moringaoleifera leaf extract. The graded levels of M. oleifera leaf extract were 0.00g (control), 0.05g, 0.10g, 0.15g, 0.20g, 0.25g per 100g for each diet. After six weeks of the feeding trial, fish previously fed each experimental diet were exposed to pathogenic strain of Aeromonashydrophila at a concentration of 9.3 × 105 CFU /mL. After bath exposure, fish from each dietary treatment was placed into the aquaria culture system. They were fed their respective diets at 5% body weight twice daily, and mortality was monitored for the remaining 4 weeks of the feeding trial. After the feeding trial, fish previously fed each experimental diet were kept in plastic tanks for a 2-hour journey. Blood and liver samples were collected for hepatocellular assessments (Aspartate transaminase (AST), Alanine transaminase (ALT), Lactate dehydrogenase (LDH) and Malate dehydrogenase (MDH) tests) and stress biomarkers (survival, cortisol and glucose). Results showed that the increases of the AST, ALT, LDH, MDH, cortisol and glucose induced by stressors were significantly reduced (P< 0.05) by supplementing the fish with M. oleifera leaf extract in the diets. Based on the result of this study, a dose of 0.10g/100g dietary Moringa leaf supplementation was sufficient as a hepatoprotective and stress reducing agent in Nile tilapia, O. niloticus.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
How libraries can support authors with open access requirements for UKRI fund...
Marine Pharmaceuticals
1. Marine Pharmaceuticals
Drugs from the Deep
Presented by: Justin Porter BIOL 4320H Winter 2012
2. Why look to the Sea?
• Biodiversity and evolution of unique defensive
compounds
• Approx. 11,000 marine compared to 155,000
terrestrial derived drugs
• New treatments for MDR
• Low toxic side effects
3. Diving into marine drug
development
• Properties discovered:
•Anti-bacterial
•Anti-coagulant
•Anti-fungal
•Anti-protozoal
•Anti-tuberculosis
•Anti-viral
5. Sponges
• Anti-viral:
•Currently on the market
•Acyclovir
•Ara-A (vidarabine)
•Ara-C (cytarabine)
•In development
•4-methylaaptamine
•EC50 = 2.4 µM
•Verongiaquinol
6. Sponges (continued)
• Anti-bacterial & Anti-fungal:
•Verongiaquinol
•MIC = 8 µg/mL
•Manzamine A
•Broad-spectrum vs Gram-positive
•Good vs Gram-negative
•Verongiaquinol and Manzamine A
are very effective antifungal agents.
7. Sponges (continued)
• Anti-protozoal & Anti-tuberculosis:
•Xestoquinone
•Anti-malarial polyketide
•Pfnek-1 kinase inhibition
•IC50 = 1.1 µM to 3 µM
•Manzamine Alkaloids
•Inhibit M. tuberculosis
•IC50 = 0.4 µg/mL to 5.2 µg/mL
•6-hydroxymanzamine E is most potent
12. Algae
• Anti-viral:
•Most antiviral compounds from marine
algae are polysaccharides
•Diterpenes from Dictyota spp.
•Inhibit HIV-1 reverse
transcriptase
•Dolabelladienetriol
•Terpenoid from Sargassum binderi
•Non-competitive inhibitor of HIV-1 RT
13. Algae (continued)
• Anti-inflammatory & Anti-fungal:
•Fucoxanthin
•Pigment from Myagropsis myagorides
•Anti-inflammatory effect rivals the current SAID
predinisolone!
•Extracts from Odonthalia corymbifera
•Potent inhibition of fungal Isocitrate Lysase
•IC50 = 23 µg/mL
•May be effective against Candida albicans
15. Fish
Anti-bacterial Properties
• Innate antimicrobial arsenal of complements, lectins and
lysozymes in the mucus and serum
• Acidic glycoprotien L-amino acid oxidase (LAO) was
isolated from Rockfish (Sebastes schlegelii)
•Specifically catalyzes L-lysine
•Induces damage and morphological changes to bacterial cell
surface
16. Fish (continued)
Anti-coagulant Properties
•Monomeric protein isolated from Yellowfin sole (Limanda
aspera) inhibited serine endopeptidase factor XII
•Controls platelet aggregation by creating an inactive
complex
18. Corals
• Anti-inflammatory:
•Pseudopterosin A
•Diterpene glycoside from Pseudopterogorgia
elisabethae (Caribbean Sea Whip)
•Anti-inflammatory effect rivals industry standards
•Used in some Estée Lauder cosmetics
20. • Many promising compounds in preclinical stages:
•TGAP
•Anticoagulant polypeptide from Tegillarca granosa
(Malaysian cockle)
•Inhibits conversion of prothrombin to thrombin
21. Future of Marine Pharmaceuticals
• Challenges:
•Accessibility
•Rarity
•Endangered or protected species and their products
require cumbersome permitting procedures
•Large-scale production
•Chemical synthesis may be a solution for some
•Acyclovir
•Cultivation using bioreactors
•Combinatorial biosynthesis
•There are many more discoveries to be made!
23. References
Aqil, F., M. Zahin, K.A. El Sayed, I. Ahmad, K.Y. Orabi, and J.M. Kijjoa A. and P. Sawangwong. 2004. Drugs and Cosmetics
Arif. 2011. Antimicrobial antioxidant, and anitmutagenic from the Sea. Mar. Drugs. 2: 73-82.
activities of selected marine natural products and Kitani Y., N. Kikuchi, G. Zhang, S. Ishizaki, K. Shimakura, K.
tobacco cembranoids. Drug and Chemical Toxicology, Shiomi and Y. Nagashima. 2008. Antibacterial action of
24(2): 167-179. L-amino acid oxidase from the skin mucus of rockfish
Berrue, F., O.P. Thomas, R. Laville, S. Prado, J. Golebiowski, R. Sebastes schlegelii. CBP Part B: Biochemistry and
Fernandez, and P. Amade. 2007. The marine sponge Molecular Biology. 149( 2): 394-400.
Plakortis zyggompha: a source of original bioactive Larghi, E.L., M.L. Bohn, and T.S. Kaufman. 2009. Aaptamine
polyketides. Tetrahedron. 63: 2328-2334. and related products. Their isolation, chemical
De Souza Pereira H., L.R. Leaso-Ferreira, N. Moussatche, V.L. syntheses, and biological activity. Tetrahedron, 65:
Telixeira , D.N. Cavalcanti, L.J. Da Costa and I.C. 4257-4282.
Fruulhetti. 2005. Effects of diterpenses isolated from Leary, D., M. Vierros, G. Hamon, S, Arico and C. Monagle.
the Brazilian marine alga Dictyota menstrualis on HIV-1 2009. Marine genetic resources: A review of scientific
reverse transcriptase. Planta Med. 71(11): 1019-24. and commercial interest. Marine Policy, 33: 183-194.
El-Amraoui, B., J.F. Biard, M.J. Uriz, S. Rifai, and A. Fassouane. Lee, J.B. , K. Hayashi, M. Hirata, E. Kuroda, E. Suzuki, Y. Kubo,
2010. Antifungal and antibacterial activity of Porifera and T. Hayashi. 2006. Antiviral sulfated polysaccharide
extracts from the Moroccan Atlantic coasts. Journal de from Navicula directa, a diatom collected from deep-
Mycologie Médicale. 20: 70-74. seawater in Toyama Bay, Biol. Pharm. Bull. 29: 2135–
Imhoff, J.F., A. Labes, and J. Wiese. 2011. Bio-mining the 2139.
microbial treasures of the ocean: New natural products. Mayer, A.M.S, A.D. Rodriguez, R.G.S. Berlinck and M.T.
Biotechnology Advances. 29: 468-482. Hamann. 2009. Marine pharmacology in 2005-6:
Jacquet, S., T. Miki, R. Noble, P. Peduzzi and S. Wilhelm. Marine compounds with anthelmintic, antibacterial,
2010. Viruses in aquatic ecosystems: important anticoagulant, antifungal, anti-inflammatory,
advancements of the last 20 years and prospects for the antimalarial, antiprotozoal, antituberculosis an antiviral
future in the field of microbial oceanography and activies; affecting the cardiovascular, immune and
limnology. Advances in Oceanography and Limnology, nervous systems, and other miscellaneous mechanisms
1(1): 97-141. of action. Biochima et Biophysica Acta, 1790: 283-308.
24. References (continued)
Mayer A.M.S., A.D. Rodriguez, R.G.S. Berlinck and N. Fusetani. Subramanian, S., N.W. Ross and S.L. Mackinnon. 2008.
2011. Marine pharmacology in 2007-8: Marine Comparison of antimicrobial activity in the epidermal
compounds with antibacterial, anticoagulant, mucus extracts of fish. CBP Part B: Biochemisty and
antifungal, anti-inflammatory, antimalarial, Molecular Biology, 150(1): 85-92
antiprotozoal, antitberculosis and antiviral activities: Villa, F.A. and L. Gerwick. 2010. Marine natural products
affecting the immune and nervous system, and other discovery: Leads for treatment of inflammation, cancer,
miscellaneous mechanisms of action. Comparative infections, and neurological disorders.
Biochemistry and Physiology, Part C. 153: 191-222. Immunopharmacology and Immunotoxicology, 32(2):
Pangestuti R. and S. Kim. 2011. Biological activities and 228-237.
health benefit effects of natural pigments derived from Vo, T., D. Ngo, Q.V. Ta and S. Kim. 2011. Marine organisms as
marine algae. Journal of Functional Foods, 3:255-266. a therapeutic source against herpes simplex virus
Qi S.H., S. Zhang, L.H. Yang and Qian P.Y. 2008. Antifouling infection. European Journal of Pharmaceutical Science,
and antibacterial compounds from the gorgians 44: 11-20.
Subergorgia gracillis. Natural Products Research, 22 (2): Wase, N.V. and P.C. Wright. 2008. Systems biology of
154-166 cyanobacterial secondary metabolite production and its
Rastogi, R.P. and R.P. Sinha. 2009. Biotechnological and role in drug discovery. Expert Opin. Drug Discov., 3(8):
industrial significance of cyanobacterial secondary 903-929.
metabolites. Biotechnology Advances, 27: 521-539. Wiliams, P.G. 2008. Planning for chemical gold: marine
Sperstad S.V., T. Haug, H. Blencke, O.B. Styrvoid, C. Li and K. bacteria as a source of new therapeutics. Trends in
Stensvag. 2011. Antimicrobial peptides from marine Biotechnology, 27(1): 45-52.
invertebrates: Chanllenges and perspectives in marine Uzair B., Z. Mahmood and S. Tabassum., 2011. Antiviral
antimicrobial peptide discovery. Biotechnology activity of natural products extracted from Marine
Advances. 29: 519-530. organisms. BioImpacts 1(4): 203-211.
Stengel, D.B., S. Connan and Z.A. Popper. 2011. Algal Yasuhara-Bell, J. and Y. Lu. 2010. Marine compounds and
chemodiversity and bioactivity: Sources of natural their antiviral activities. Antiviral Research, 66: 231-240
variability and implications for commercial application.
Biotechnology Advances, 29: 483-501.
25. References (continued)
Mayer A.M.S., A.D. Rodriguez, R.G.S. Berlinck and N. Fusetani. Subramanian, S., N.W. Ross and S.L. Mackinnon. 2008.
2011. Marine pharmacology in 2007-8: Marine Comparison of antimicrobial activity in the epidermal
compounds with antibacterial, anticoagulant, mucus extracts of fish. CBP Part B: Biochemisty and
antifungal, anti-inflammatory, antimalarial, Molecular Biology, 150(1): 85-92
antiprotozoal, antitberculosis and antiviral activities: Villa, F.A. and L. Gerwick. 2010. Marine natural products
affecting the immune and nervous system, and other discovery: Leads for treatment of inflammation, cancer,
miscellaneous mechanisms of action. Comparative infections, and neurological disorders.
Biochemistry and Physiology, Part C. 153: 191-222. Immunopharmacology and Immunotoxicology, 32(2):
Pangestuti R. and S. Kim. 2011. Biological activities and 228-237.
health benefit effects of natural pigments derived from Vo, T., D. Ngo, Q.V. Ta and S. Kim. 2011. Marine organisms as
marine algae. Journal of Functional Foods, 3:255-266. a therapeutic source against herpes simplex virus
Qi S.H., S. Zhang, L.H. Yang and Qian P.Y. 2008. Antifouling infection. European Journal of Pharmaceutical Science,
and antibacterial compounds from the gorgians 44: 11-20.
Subergorgia gracillis. Natural Products Research, 22 (2): Wase, N.V. and P.C. Wright. 2008. Systems biology of
154-166 cyanobacterial secondary metabolite production and its
Rastogi, R.P. and R.P. Sinha. 2009. Biotechnological and role in drug discovery. Expert Opin. Drug Discov., 3(8):
industrial significance of cyanobacterial secondary 903-929.
metabolites. Biotechnology Advances, 27: 521-539. Wiliams, P.G. 2008. Planning for chemical gold: marine
Sperstad S.V., T. Haug, H. Blencke, O.B. Styrvoid, C. Li and K. bacteria as a source of new therapeutics. Trends in
Stensvag. 2011. Antimicrobial peptides from marine Biotechnology, 27(1): 45-52.
invertebrates: Chanllenges and perspectives in marine Uzair B., Z. Mahmood and S. Tabassum., 2011. Antiviral
antimicrobial peptide discovery. Biotechnology activity of natural products extracted from Marine
Advances. 29: 519-530. organisms. BioImpacts 1(4): 203-211.
Stengel, D.B., S. Connan and Z.A. Popper. 2011. Algal Yasuhara-Bell, J. and Y. Lu. 2010. Marine compounds and
chemodiversity and bioactivity: Sources of natural their antiviral activities. Antiviral Research, 66: 231-240
variability and implications for commercial application.
Biotechnology Advances, 29: 483-501.
Marine Environment = ~1/2 of global biodiversity ( Sperstad et al. , 2011; Vo et al ., 2011; Yasuhara-Bell and Lu, 2010) Oceans contain 34 of the 36 phyla ~300,000 described species (small % of what is out there, since only about 3% of ocean has been explored) Estimated between 3 million and 500 million species Coral reefs alone rival the biodiversity of tropical rainforests Deep sea hydrothermal vents Abyssal plain (not barren) Plankton (global) Many extreme environments --> novel compounds The sea is rich in Biodiversity and having to live under extreme conditions has resulted in the evolution of very unique defences to survive. Marine Pharmaceuticals are in their infancy compared to terrestrial Pharmaceuticals. about 11,000 marine derived pharmaceuticals compared to 155,000 terrestrial products. (2009) The search for new drugs, therapeutic agents and natural products is crucial, especially with the rise of antibiotic resistant and multi-drug resistant (MDR) bacteria, fungi, protozoa and viruses To improve the medicine and treatment of ailments with compounds that have low toxic side effects. Example fungicides and anti-mycobaterial for tuberculosis treatments very toxic and take 6-9months of use.
It all started with sponges in 1959. In 1959, was the first study of sponges producing antimicrobial substances. Since then there has been a boom in marine exploration for new chemical compounds used to combat infectious diseases Exploration of numerous species of sponges, fungi, fish, bacteria, algae and other invertebrates compounds that have shown anti-bacterial, anti-coagulant, anti-fungal, anti-inflammatory, anti-protozoal, anti-tuberculosis and anti-viral properties
There have been more than 15,000 products derived from more than 5,300 different compounds from marine sponges (Vo et al. , 2011; Yasuhara-Bell and Lu, 2010). It is no surprise that sponges are a significant source of pharmacologically active agents, given their location and role in the marine environment Sponges (Porifera) are benthic stationary filter feeding organisms that "clean" the marine environment
Anti-Viral: Many antiviral agents have been derived from marine sponges Currently on the market: Acyclovir - Synthetic derivative of arabinosyl nucleosides from sponge, Tethya cripta - HSV Inhibits HSV-1 replication EC50 = 8.6 µM Ara-A (vidarabine) /Ara-C (cytarabine) - HSV Alkaloid - 4-methylaaptamine from sponge Aaptos aaptos Inhibits HSV-1 replication EC50 = 2.4 µM (Better than acyclovir!) Verongiaquinol (Aquil et al. , 2011) Anti-HIV-1 activity (ED50 = 6.1 µM)
Anti-Bacterial : (Aquil et al. , 2011) Over 800 antibacterial compounds have been isolated from sponges (El-Amraoui et al. , 2010) Verongioquinol from Red Sea sponge Aplysina spp. (minimum IC = 8 µg/mL) Manzamine A from Indo-Pacific Acanthostrongylophora spp. Manzamines are polycyclic alkaloids Very promising Has shown broad-spectrum activity vs Gram-positive bacteria Moderate-good activity vs Gram-negative bacteria Anti- Fungal: Verongioquinol and Manzamine A
Anti-Protozoal (Gutiérrez et al. , 2005; Kaur et al. , 2009; Mayer et al ., 2009) Parasitic diseases: Malaria ( Plasmodium falciparum ), leishmaniasis, Chagas disease (Trypanosoma cruzi ) , dengue fever Xestoquinone is polyketide anti-malarial agent from Xestospongia spp. Pfnek-1 kinase inhibition (IC 50 = 1.1 µM to 3 µM) P. falciparum NIMA (never-in-mitosis)-related kinase. – Prevents asexual reproduction Anti-Tuberculosis [ Mycobacterium tuberculosis ] ( Berrué et al. , 2007; Mayer et al. , 2009; Mayer et al. , 2011) No known mechanisms of antimycobacterial action for marine compounds. Manzamine Alkaloids (Kishore et al. , 2009; Rao et al. , 2006) Inhibits M. tuberculosis with IC50 = 0.4-5.2 µg/mL 6-hydroxymanzamine E (IC50 = 0.4 µg/mL) Manzamine A (IC50 = 1.5 µg/mL) Manzamine F (IC50 = 2.6 µg/mL) Manzamine E (IC50 = 3.8 µg/mL) Manzamine Y (IC50 = 5.2 µg/mL) Spiculoic Acids (Berrué et al. , 2007) 24-Norisospiculoic acid A - antimycobacterial effect on M. tuberculosis with minimum IC99 = 50 µg/mL
Many bacterial species permanently inhabit sponges as symbionts. In some cases, associated bacteria might be the producers of the isolated pharmaceutically active compounds from sponges.
Marine fungi have been found with: Antiviral antibacterial Antifungal properties (Vo et al. , 2011; Yasuhara-Bell and Lu, 2011)
Antiviral Linear peptides - Halovirs A-E isolated from marine fungus Scytidium spp. Halovir A inhibits HSV-1 and HSV-2 with EC50 = 280 nM in standard plaque reduction assay Render HSV non-infectious by membrane destabilisation (Vo et al. , 2011) Antibacterial Corollosporine - antibacterial metabolite from Corollospora maritime (Yasuhara-Bell and Lu, 2011).
Dictyota = Brown algae (macroalgae) Sargassum binderi = Brown macroalgae
Anti-inflammatory Pigment - Fucoxanthin from Myagropsis myagroides (Pangestuti and Kim, 2011) Anti-inflammatory effect rivals the current steroidal anti-inflammatory drug (SAID) predinisolone Antifungal: Odonthalia corymbifera (Lee et al. , 2007) Red macroalga. Inhibits fungal Isocitrate Lysase required for glyoxylate cycle Most dangerous human pathogenic fungus = Candida albicans Potent inhibition of ICL (IC50 = 23 µg/mL)
The Mucus and serum found on fish serve as a fish’s first line of defence against pathogens. Recently, there has been great interest in fish as a source for novel bacterialcides Studies on the mucus extracts exhibit inhibition of microbial activity for both fish and human pathogens (Subramanian et al. 2008) e.g. salmonella. Mucus extracts were taken from haddock, arctic Char, hagfish and brook trout. Isolated from the Serum of rockfish was 120kDa acidic glycoprotein L-amino acid oxidase (LAO). Which selectively targeted and inhibited gram-negative bacteria (MIC=0.078-0.63 µg/mL). Using an electron microscope it was found that the protein induced damage and morphological changes to the surface of the bacterial cells. The effectiveness of the antibacterial action was controlled by H 2 O 2. (Mayer et al. 2011). LAO specifically catalyzes L-lysine with a K m 0.37 mM and k cat 57.1 s − 1 serum LAO exhibited antibacterial activity potently against Aeromonas hydrophila and Aeromonas salmonicida, which are disease associated with Cholera in infants and children with compromised immune systems, but it is commonly a persistent disease for amphibians, fish and reptiles. Commonly known by fish enthusiasts as Fin and Tail Rot. Very promising results for a novel agent especially for aquariums and veterinary Medicine.
-In the marine yellowfin sole ( Limanda aspera ) was discovered a 12.01 kDa single-chain monomeric protein which inhibited the blood coagulation serine endopeptidase factor XII. -It accomplishes this by creating an inactive complex by binding to a membrane glycoprotein intergrin, which stimulates platelet aggregation.
Corals are Cnidarians Relatives of jellyfish and sea anemones
Anti-inflammatory - Pseudopterosin A from Pseudopterogorgia elisabethae [Gorgonian soft coral, Octocoral] Has been used by Estée Lauder in cosmetic products
Molluscs - Clams, mussels, oysters, abalone, snails, sea slugs, cephalopods) Crustaceans Echinoderms – Sea urchins, sea stars, brittle stars, sea cucumbers, sand dollars T. granosa Anti-coagulant polypeptide (TGAP) from Bivalve ( Tegillarca granosa ) “Malaysian cockle” or “Granulated ark” Inhibition of prothrombin (factor II) to thrombin ( IIa) conversion IC 50 = 77.9 nM Specific binding to factor Va and prothrombin (factor II)
Compare Pros and cons of Marine pharmaceuticals Challenges Large-scale production Chemical synthesis may be a solution for some e.g. Acyclovir Cultivation using bioreactors Combinatorial biosynthesis