ntroduction of Lipids,Chemistry, Structural elucidation of Essential Fatty acid. Prostaglandins, Vitamin A, Phospolipids ,Cholesterol, Lanosterol its synthesis
ntroduction of Lipids,Chemistry, Structural elucidation of Essential Fatty acid. Prostaglandins, Vitamin A, Phospolipids ,Cholesterol, Lanosterol its synthesis
Since food is both important for physical and mental well being, the importance of food in life is huge. Proper nutrition means that you get all the essential nutrients required for healthy functioning of the body through your diet. ... These nutrients include vitamins and minerals, fats, carbohydrates, and proteins.
This slide will provide you information about carbohydrates, their classification and functions. Optical activity , epimers, anomers and isomers have also been discussed in the slides.
Lipids (Greek: lipos, means fat or lard)
- are a heterogeneous class of naturally occurring organic substances
- have a distinguished functional group or structural features
- are insoluble in water and highly soluble in one or more of the solvents: ether, chloroform, benzene and acetone.This property sets them apart from proteins, carbohydrates,, nucleic acids and other biomolecules
- are widely distributed in the biological world
- play a wide variety of roles in plant and animal tissues
Since food is both important for physical and mental well being, the importance of food in life is huge. Proper nutrition means that you get all the essential nutrients required for healthy functioning of the body through your diet. ... These nutrients include vitamins and minerals, fats, carbohydrates, and proteins.
This slide will provide you information about carbohydrates, their classification and functions. Optical activity , epimers, anomers and isomers have also been discussed in the slides.
Lipids (Greek: lipos, means fat or lard)
- are a heterogeneous class of naturally occurring organic substances
- have a distinguished functional group or structural features
- are insoluble in water and highly soluble in one or more of the solvents: ether, chloroform, benzene and acetone.This property sets them apart from proteins, carbohydrates,, nucleic acids and other biomolecules
- are widely distributed in the biological world
- play a wide variety of roles in plant and animal tissues
Lipids (Greek letter: lipos means fat) are substances of biological origin that are soluble in organic solvents such as chloroform and methanol but are only sparingly soluble, if at all, in water.
Fatty Acids
Waxes
Triacylglycerols
Phosphoglycerides
Sphingolipids
Sterols And Terpenes
This presentation intends to offer a bird's eye view of bio-molecules in general and lipids in particular along with its beneficial and harmful attributes.
Biochemistry or biological chemistry is the study of chemical processes within and relating to living organisms. Biochemistry is the application of chemistry to the study of biological processes at the cellular and molecular level. It emerged as a distinct discipline around the beginning of the 20th century when scientists combined chemistry, physiology, and biology to investigate the chemistry of living systems.
Introduction
History
Tumor suppressor gene- pRB
- RB gene
- Role of RB in regulation of cell cycle
- Tumor associated with RB gene mutation
Tumor suppressor gene- p53
- What is p53 gene?
- Function of p53 gene
- How it regulates cell cycle
- What happen if p53 gene inactivated
- Cancer associated with p53 mutation
- Conclusion
- References
Introduction
Definition
History
Two hit hypothesis
Functions
Mutation in tumor suppressor genes
What is mutation
Inherited mutation of TSGs
Acquired mutation of TSGs
What is Oncogenes?
TSGs and Oncogenes : Brakes and accelerators
Stop and go signal
Examples of TSGs:
RB-The retinoblastoma gene
P53 protein
TSGs &cell suicide
Conclusion
References
Introduction
Protein synthesis
Synthesis of secretory proteins on membrane-bound ribosomes
Processing of newly synthesized proteins in the ER
Synthesis of integral membrane protein on membrane bound ribosomes
Maintenance of membrane asymmetry
Conclusion
Reference
Introduction
Definition
Factors required for Translation
Formation of aminoacyl t-RNA
1)Activation of amino acid
2) Transfer of amino acid to t-RNA
Translation involves following steps:-
1)Initiation
2)Elongation
3)Termination
Conclusion
Reference
Introduction
Definition
History
central dogma
Major components
mRNA,tRNA,rRNA
Energy source
Amino acids
Protien factor
Enzymes
Inorganic ions
Step involves in translation:
Aminoacylation of tRNA
Initiation
Elongation
termination
Importance of translation
Conclusion
Reference
Introduction
Protein modifications
Folding
Chaperon mediated
Enzymatic
Cleavage
Addition of functional groups
Chemical groups
Hydrophobic groups
Proteolysis
Conclusion
Reference
INTRODUCTION
HISTORY
WHAT IS TRANSCRIPTION
PROKARYOTIC TRANSCRIPTION
STEPS OF TRANSCRIPTION
HOW TRANSCRIPTION OCCURS
PROCESS OF TRANSCRIPTION
Initiation
Elongation
Termination
CONCLUSION
REFRENCES
Enzyme Kinetics and thermodynamic analysisKAUSHAL SAHU
Introduction
Kinetics and thermodynamicSG
Thermodynamic in enzymatic reactions
balanced equations in chemical reactions
changes in free energy determine the direction & equilibrium state of chemical reactions
the rates of reactions
Factors effecting enzymatic activity
(i) Enzyme concentration.
(ii) Substrate concentration.
(iii)Temperature
(iv) pH.
(v) Activators.
(vi)Inhibitors
Michaelis-menten equation
CONCLUSIONS
REFERENECES
Recepter mediated endocytosis by kk ashuKAUSHAL SAHU
INTRODUCTION
DEFINITION OF RECEPTOR MEDIATED ENDOCYTOSIS
WHAT TYPE OF LIGANDS ENTER BY RME?
FORMATION OF CLATHRIN-COATED VESICLES
TRISKELIONS
ROLE OF DYNAMIN IN THE FORMATION OF CLATHRIN-COATED VESICLES
ROLE OF PHOSPHOLIPIDS IN THE FORMATION OF COATED VESICLES
ENDOCYTIC PATHWAY
LDLs AND CHOLESTROL METABOLISM
CONCLUSION
REFERENCES
The delivery of newly synthesized protein to their proper cellular destination, usually referred to as protein targeting or sorting.
The mode of protein transport depends chiefly on the location in the cell cytoplasm of the polysomes involved in protein synthesis.
There are two modes of protein sorting:-
1) Co - translational Transportation.
2) Post - translational Transportation.
Prokaryotic translation machinery by kk KAUSHAL SAHU
Introduction
Definition
Factors required for Translation
Formation of aminoacyl t-RNA
1)Activation of amino acid
2) Transfer of amino acid to t-RNA
Translation involves following steps:-
1)Initiation
2)Elongation
3)Termination
Conclusion
Reference
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
1. STORAGE LIPID
.
By
KAUSHAL KUMAR SAHU
Assistant Professor (Ad Hoc)
Department of Biotechnology
Govt. Digvijay Autonomous P. G. College
Raj-Nandgaon ( C. G. )
3. SOME IMPORTANT TERMS
ADIPOCYTES - storage cell.
LIPASE- enzyme that hydrolyze neutral fats.
LEPTIN- hormone that regulate fat in body.
LIPOPROTEIN- protein bind with lipid and act as a carrier.
4. INTRODUCTION AND HISTORY
The term lipid was used for the first time by
Bloor in 1943.
Lipids are heterogeneous group of compounds
related to fatty acids and include fats, oils, waxes
and other related substances.
CLASSIFICATION
A.Simple lipids or Homolipids
B. Compound lipids or Heterolipids.
C. Derived lipids
Electron micrograph of a thin section of a fat
storage cell or adipocyte
5. STORAGE LIPIDS
• Derivatives of fatty acids.
• Low oxidation state (that is, as highly reduced)
• The cellular oxidation of fatty acids (to CO2 and H2O) is highly exergonic.
Two types of fatty acid–containing compounds:
FATS AND OILS (NATURAL LIPID AND TRIGLYCERIDES )
•most abundant
•constitute about 98% of total dietary lipids
•are nonpolar, hydrophobic molecules since they contain no electrically charged or
highly polar functional groups.
•In animals, adipocytes contain very large quantities of triglycerides.
• Adipocytes are abundantly found under the skin, in the abdominal cavity and in the
mammary glands.
6. Human body contains enough
fat to make 7 bars of soap !
In a normal man, weighing 70
kg, at least 10-20% of the body
weight is lipid, the bulk of
which is triacylglycerol (TAG).
Yield twice much more energy
than cabohydrates.
7. Saturated Fatty Acids
All carbon atom are interlinked by single bonds and each carbon atom carries a
hydrogen atom.
They cannot take up any more hydrogen, hence saturated. They have straight chains.
They have a general formula CnH2nO2 .
Unsaturated Fatty Acids
Two adjacent carbon atom at one or more places lack hydrogen atom and are
interlinked by double bond (C=C). they can take up additional hydrogen, hence called
unsaturated.
They have general formula CnH2n-2xO2.
8.
9. WAXES
The term “wax” originates from the Old English weax, meaning “the material of the
honeycomb.
Biological waxes are esters of long-chain (C14 to C36) saturated and unsaturated fatty
acids with long-chain (C16 to C30) alcohols.
Their melting points (60 to 100 _C) are generally higher
In plankton, the free-floating microorganisms at the bottom of the food chain for
marine animals, waxes are the chief storage form of metabolic fuel.
Lanolin (from lamb’s wool), beeswax ,
carnauba wax (from a Brazilian palm tree), and
wax extracted from spermaceti oil are widely used in
the manufacture of lotions, ointments, and polishes.
A honeycomb, constructed of
beeswax, is firm at 25_C .
10. Processing of Dietry lipid in vertebrates
Digestion and absorption of dietry lipid occur in small intestine and the fatty acid released from triglycerol
are packaged and delivered to muscles and adipose tissue.
11. FUNCTIONS OF STORAGE LIPID
RESERVE FOOD MATERIAL-
They are stored in special regions – connective around the kidney, under the dermis
in mammals , in the fat bodies in frog and lizards and in the liver in fish . The adipose
tissue are specialize in storing fat droplets.
In plants oil is stored in the seeds to provide nourisment to the germinating
embyro.
a) FUEL
b) INSULATION COATS
c) SOURCE OF WATER
Waxes form a water-proof protecting coating on animal fur and feather,on plant
stem,leaves and fruit.
12. BIOLOGICAL ROLES OF LIPIDS
(a) The fat under the skin of
this elephant seal protect it
from cold.
(b) kidneys are embedded in
fatty tissue.
(c) The wax suface of leaves repel water
but also reduce water loss from plant.
(d) Every cell membrane
contain lipid.
15. Lipid storage disorders
I. Lipid storage diseases, or the lipidoses, are a group of inherited metabolic
disorders in which harmful amounts of fatty materials (lipids) accumulate in
various cells and tissues in the body.
II. People with these disorders either do not produce enough of one of the enzymes
needed to break down (metabolize) lipids or they produce enzymes that do not
work properly.
III. Inherited from one or both parents.
Gaucher disease- caused due to deficiency of the enzyme glucocerebrosidase
Niemann-Pick disease- caused due to accumulation of fat and cholesterol in cells of
the liver, spleen, bone marrow, lungs, and, in some patients, brain.
16. CONCLUSIONS
* Almost all fatty acids, the hydrocarbon components of many lipids, have an even
number of carbon atoms (usually 12 to 24); they are either saturated or unsaturated,
with double bonds almost always in the cis configuration.
* Triacylglycerols contain three fatty acid molecules esterified to the three hydroxyl
groups of glycerol. Simple triacylglycerols contain only one type of fatty acid; mixed
triacylglycerols, two or three types.
* Triacylglycerols are primarily storage fats;they are present in many foods.
17. REFERENCES
BOOKS
1. PRINCIPLES OF BIOCHEMISTRY 5TH EDITION BY David L. Nelson and Michael M. Cox,
2. Harper's Illustrated Biochemistry (Murray, McGraw-Hill Medical, 26th Ed),
3. Fundamental of biochemistry by H.P Gareja and S.V Patel.,1st Ed.,
4. Basic concept of biochemistry by Hiram F. Gilbert, 2nd Ed.,
5. Biochemistry by J.L Jain.
WEBSITES
www.NINDS.com (26 /08/13, 6pm to 8pm )
www.ask.com (26 /08/13, 6pm to 8pm )
www.livestrong.com (28/08/13,7pm-8pm)
www.beltina.org (28/08/13,7pm-8pm)