Stem cells can be obtained from embryos or adults. Embryonic stem cells are pluripotent and can become any cell type, while adult stem cells are multipotent and limited to certain lineages. Stem cell research offers promise for therapies but also ethical concerns. Alternatives to embryonic stem cells are being explored, such as stem cells from unfertilized eggs, dead embryos, or engineered structures. While progress is being made, many challenges remain before stem cell therapies can be directly translated from the laboratory.
Stem Cell Technology and its Clinical ApplicationDr. Barkha Gupta
Dr. Barkha Gupta has been teaching Veterinary Biochemistry as well as clinical physiology at CVAS, Udaipur and PGIVER, Jaipur. She has earlier served in various capacities in the Department of Animal Husbandry, Govt. of Rajasthan. She has several publications and awards to her credit. She is the PI of M-RAJUVAS Android Educational Mobile Application for Veterinary and Animal Sciences and Kiosk Information System for Farmers/Livestock Owners. Dr. Gupta is also IFBA Certified Professional.
Stem Cell Technology and its Clinical ApplicationDr. Barkha Gupta
Dr. Barkha Gupta has been teaching Veterinary Biochemistry as well as clinical physiology at CVAS, Udaipur and PGIVER, Jaipur. She has earlier served in various capacities in the Department of Animal Husbandry, Govt. of Rajasthan. She has several publications and awards to her credit. She is the PI of M-RAJUVAS Android Educational Mobile Application for Veterinary and Animal Sciences and Kiosk Information System for Farmers/Livestock Owners. Dr. Gupta is also IFBA Certified Professional.
If the cell is able to form all cell types of the embryo & adult (Fertilized egg cell) Totipotent stem cell
Stem cell able to differentiate into all 3 germ layers Pluripotent stem cell (Embryonic stem cell)
Multipotent stem cell Differentiate to form cells of some but not all 3 germ layers (Bone, cartilage, connective tissue)
Unipotent stem cell Able to form just one other cell type (Spermatogonia)
Embryos created in vitro fertilization
Aborted embryos
Limited tissues (bone marrow, muscle, brain)
Discrete populations of adult stem cells generate replacements for cells that are lost through normal wear and tear, injury or disease
Placental cord
Baby teeth
Diabetes patients lose the function of their insulin-producing beta cells of the pancreas
Human embryonic stem cells may be grown in cell cultures and stimulate to form insulin-producing cells , that can be transplanted into the patients
Pancreas is digested with collagenase that frees islets from surrounding cells
Centrifugation of isolates containing mainly alpha and beta cells, purified islets beta cells
Transplanted through a catheter into the liver where they become permanently established Caused when key brain cells that produce message carrying chemical/neurotransmitter (dopamine) die off.
Symptoms start with the patients trembling and can end up paralyzed
Harvesting of stem cells from patients bone marrow, foetus or any other source
Culturing of harvested stem cells in lab conditions - to get high concentrations of stem cells
Then purified and high concentration of stem cells are surgically injected in the brain of patient.
Induced Pluripotent Stem Cell & Cell Dedifferentiation: The Breakthrough of S...Vincentsia Vienna
The phenomenon of cell dedifferentiation is yet one promising trend to explore. In future, the science fiction of regenerative medicine could be turned into reality.
The use of stem cells in office procedures as the practice of medicine is commercializing in the USA, there are risks that arise because of this, but there are also benefits that are possible. Regulation is needed, but barring an MD and its patient from these procedures simply pushes them outside of the country or into incognito modes. This is where true danger arises, a path to expeditiously and ethically practice should be established where the patient consent is true, and the doctor is enhanced in his practice rather than tied down.
Stem Cell Therapy Clinical Trial at Patients MedicalPatients Medical
Dr. Kamau Kokayi from the New York Stem Cell Treatment Center at Patients Medical gives the latest information on the amazing discoveries and healing capacity of stem cells and details on enrolling in the current clinical trial at NYSCTC.
Stem Cells and Tissue Engineering: past, present and futureAna Rita Ramos
Tissue engineering brings together the principles of the life sciences and medicine with engineering. New biomaterials; advances in genomics and proteomics and increased understanding of healing processes contributed to the increase of this area over the past decade.
Stem cell biology is paving the way for the generation of unlimited cells of specific phenotypes for incorporation
into engineered tissue constructs.
Pluripotent Stem Cells and their applications in disease modelling, drug disc...tara singh rawat
This ppt gives an insight of the potential and possibilities of pluripotent stem cells research in disease modelling, drug discovery and regenerative medicine
If the cell is able to form all cell types of the embryo & adult (Fertilized egg cell) Totipotent stem cell
Stem cell able to differentiate into all 3 germ layers Pluripotent stem cell (Embryonic stem cell)
Multipotent stem cell Differentiate to form cells of some but not all 3 germ layers (Bone, cartilage, connective tissue)
Unipotent stem cell Able to form just one other cell type (Spermatogonia)
Embryos created in vitro fertilization
Aborted embryos
Limited tissues (bone marrow, muscle, brain)
Discrete populations of adult stem cells generate replacements for cells that are lost through normal wear and tear, injury or disease
Placental cord
Baby teeth
Diabetes patients lose the function of their insulin-producing beta cells of the pancreas
Human embryonic stem cells may be grown in cell cultures and stimulate to form insulin-producing cells , that can be transplanted into the patients
Pancreas is digested with collagenase that frees islets from surrounding cells
Centrifugation of isolates containing mainly alpha and beta cells, purified islets beta cells
Transplanted through a catheter into the liver where they become permanently established Caused when key brain cells that produce message carrying chemical/neurotransmitter (dopamine) die off.
Symptoms start with the patients trembling and can end up paralyzed
Harvesting of stem cells from patients bone marrow, foetus or any other source
Culturing of harvested stem cells in lab conditions - to get high concentrations of stem cells
Then purified and high concentration of stem cells are surgically injected in the brain of patient.
Induced Pluripotent Stem Cell & Cell Dedifferentiation: The Breakthrough of S...Vincentsia Vienna
The phenomenon of cell dedifferentiation is yet one promising trend to explore. In future, the science fiction of regenerative medicine could be turned into reality.
The use of stem cells in office procedures as the practice of medicine is commercializing in the USA, there are risks that arise because of this, but there are also benefits that are possible. Regulation is needed, but barring an MD and its patient from these procedures simply pushes them outside of the country or into incognito modes. This is where true danger arises, a path to expeditiously and ethically practice should be established where the patient consent is true, and the doctor is enhanced in his practice rather than tied down.
Stem Cell Therapy Clinical Trial at Patients MedicalPatients Medical
Dr. Kamau Kokayi from the New York Stem Cell Treatment Center at Patients Medical gives the latest information on the amazing discoveries and healing capacity of stem cells and details on enrolling in the current clinical trial at NYSCTC.
Stem Cells and Tissue Engineering: past, present and futureAna Rita Ramos
Tissue engineering brings together the principles of the life sciences and medicine with engineering. New biomaterials; advances in genomics and proteomics and increased understanding of healing processes contributed to the increase of this area over the past decade.
Stem cell biology is paving the way for the generation of unlimited cells of specific phenotypes for incorporation
into engineered tissue constructs.
Pluripotent Stem Cells and their applications in disease modelling, drug disc...tara singh rawat
This ppt gives an insight of the potential and possibilities of pluripotent stem cells research in disease modelling, drug discovery and regenerative medicine
“Stem Cell, Possibilities And Utility In Health sector” Ajit Tiwari
The role of stem cells in basic biological processes in vivo, namely in development, tissue repair and cancer.
Remarkable progress has been achieved in studying stem cells. The most exciting use of cultured stem cells is the promise for curing many devastating diseases like Parkinson's and diabetes. However, more basic research remains before stem-cell based therapy is widely used.
ES cells have the most capacity to differentiate into a variety of cells and their proliferation capacity is also unsurpassed by any other cell type. There are three major problems with ES cells; ethical issues, immunological rejection problems and the potential of developing teratomas.
In the future, ideally, somatic stem cells from the patient will be extracted and manipulated and then reintroduced into the same patient to cure debilitating diseases.
stem cell research is yet to be advanced , once fully developed can alleviate human suffering, this ppt reviews the contemporary evidence, pitfalls and challenges
STEM CELLS ARE THE UNDIFFERENTIATED CELLS LATER THEIR DIFFERENTIATION TAKES PLACE WHICH LET THEM TO CONVERT INTO SPECIALIZED CELLS CALLED AS STEM CELLS.
Stem cell technology | Presented by pranjali V. Bhadanepranjali bhadane
This presentation is related to biological topic stem cell technology. stem cell technology plays an important role in life science. stem cell technology is most important for future researchers on personal genomics, personal drug and Complex Disease Research...
Elevating Tactical DDD Patterns Through Object CalisthenicsDorra BARTAGUIZ
After immersing yourself in the blue book and its red counterpart, attending DDD-focused conferences, and applying tactical patterns, you're left with a crucial question: How do I ensure my design is effective? Tactical patterns within Domain-Driven Design (DDD) serve as guiding principles for creating clear and manageable domain models. However, achieving success with these patterns requires additional guidance. Interestingly, we've observed that a set of constraints initially designed for training purposes remarkably aligns with effective pattern implementation, offering a more ‘mechanical’ approach. Let's explore together how Object Calisthenics can elevate the design of your tactical DDD patterns, offering concrete help for those venturing into DDD for the first time!
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
Key Trends Shaping the Future of Infrastructure.pdfCheryl Hung
Keynote at DIGIT West Expo, Glasgow on 29 May 2024.
Cheryl Hung, ochery.com
Sr Director, Infrastructure Ecosystem, Arm.
The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
Generating a custom Ruby SDK for your web service or Rails API using Smithyg2nightmarescribd
Have you ever wanted a Ruby client API to communicate with your web service? Smithy is a protocol-agnostic language for defining services and SDKs. Smithy Ruby is an implementation of Smithy that generates a Ruby SDK using a Smithy model. In this talk, we will explore Smithy and Smithy Ruby to learn how to generate custom feature-rich SDKs that can communicate with any web service, such as a Rails JSON API.
Smart TV Buyer Insights Survey 2024 by 91mobiles.pdf91mobiles
91mobiles recently conducted a Smart TV Buyer Insights Survey in which we asked over 3,000 respondents about the TV they own, aspects they look at on a new TV, and their TV buying preferences.
The Art of the Pitch: WordPress Relationships and SalesLaura Byrne
Clients don’t know what they don’t know. What web solutions are right for them? How does WordPress come into the picture? How do you make sure you understand scope and timeline? What do you do if sometime changes?
All these questions and more will be explored as we talk about matching clients’ needs with what your agency offers without pulling teeth or pulling your hair out. Practical tips, and strategies for successful relationship building that leads to closing the deal.
State of ICS and IoT Cyber Threat Landscape Report 2024 previewPrayukth K V
The IoT and OT threat landscape report has been prepared by the Threat Research Team at Sectrio using data from Sectrio, cyber threat intelligence farming facilities spread across over 85 cities around the world. In addition, Sectrio also runs AI-based advanced threat and payload engagement facilities that serve as sinks to attract and engage sophisticated threat actors, and newer malware including new variants and latent threats that are at an earlier stage of development.
The latest edition of the OT/ICS and IoT security Threat Landscape Report 2024 also covers:
State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
Builder.ai Founder Sachin Dev Duggal's Strategic Approach to Create an Innova...Ramesh Iyer
In today's fast-changing business world, Companies that adapt and embrace new ideas often need help to keep up with the competition. However, fostering a culture of innovation takes much work. It takes vision, leadership and willingness to take risks in the right proportion. Sachin Dev Duggal, co-founder of Builder.ai, has perfected the art of this balance, creating a company culture where creativity and growth are nurtured at each stage.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
Encryption in Microsoft 365 - ExpertsLive Netherlands 2024Albert Hoitingh
In this session I delve into the encryption technology used in Microsoft 365 and Microsoft Purview. Including the concepts of Customer Key and Double Key Encryption.
Encryption in Microsoft 365 - ExpertsLive Netherlands 2024
Inman_Stem_Cells_05
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4. Early Development Fertilized egg Totipotent stem cells Totipotent : Can become any cell in body or placenta Fate Decision Pluripotent stem cells Blastocyst Pluripotent : Can become any cell in body Implantation Gastrulation Fate Decision Multipotent stem cells Primary Germ Cells Endoderm (inner) Mesoderm (middle) Ectoderm (outer) Multipotent : Can become any cell within a specific germ layer or cell lineage Embryonic stem cells come from inner cell mass of blastocyst.
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6. Is that an Embryonic Stem Cell? Embryonic stem cells injected into a SCID mouse will grow into teratomas, tumors of the germ cell layers. Individual ESCs under the correct conditions will make many different cell types. The true potential of stem cells can only be assessed retrospectively SCID Mouse: Severe Combined ImmunoDeficiency
7. Stem Cells: From Embryonic to Adult Embryonic stem cells are those removed from the blastocyst before the fate decision from pluripotentiality to multipotentiality. Adult stem cells are those multipotential cells that persist in fully developed tissues. These cells never differentiated into the mature cell types of the tissues in which they reside. http://www.brown.edu/Courses/BI0032
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9. Adult Stem Cells – Bone Marrow NIH: stemcells.nih.gov/ info/basics/basics4.asp Major repository of adult stem cells -Hematopoeitic -Mesenchymal Give rise to immune system cells Constant turnover
10. Stem Cell Phenotype Fate dictated by environment… Shihabuddin, et al., J. Neuroscience 20(23) 8727-8735, 2000 Neural stem cells Neurons Astrocytes Oligodendrocytes Oligodendrocyte Progenitor Cells Stem cells placed in brain become neurons… Stem cells placed in spinal cord become glial cells…
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14. Culturing Embryonic Stem Cells Obtain stem cells from 1. Remove inner cell mass 2. Put cells in dish with feeder layer 3. Cells divide Somatic Cell Nuclear Transfer Oocyte without nucleus Inject nucleus from adult somatic cell Blastocyst Fertilized egg Blastocyst + Sperm Oocyte In Vitro Fertilization
![Stem Cells in Research ,[object Object],Denise Inman, PhD University of Washington Department of Neurosurgery](https://image.slidesharecdn.com/1181453/85/Inman_Stem_Cells_05-1-320.jpg)
