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IB Biology
3 Chemistry of Life
3.2 Carbohydrates, Lipids and Proteins
All syllabus statements ©IBO 2007
All images CC or public domain or link to original material.

Jason de Nys




                             http://commons.wikimedia.org/wiki/File:Burger_King_Angus_Bacon_%26_Cheese_Steak_Burger.jpg
Carbs


 Rabbit food




Proteins
& Fats
                 Fats
3.2.1 Distinguish between organic and inorganic compounds


          Organic molecules are based on carbon and are
                         in living things.

            Inorganic compounds are everything else plus
              carbonates (e.g. HCO3⁻ and CaCO3), carbon
               dioxide (CO2 )and carbon monoxide (CO).




                                                     http://commons.wikimedia.org/wiki/File:GraphiteUSGOV.jpg
3.2.2 Identify amino acids, glucose*, ribose* and fatty acids from diagrams showing their
structure
           Alanine                          Arginine                            Leucine




  Here are three of
  the twenty-one
  amino acids found
  in eukaryotes.
  Identify what parts
  of their structures
  are identical.
                                                              http://commons.wikimedia.org/wiki/File:Alanine.png
                                                             http://commons.wikimedia.org/wiki/File:Arginine.png
                *glucose and ribose shown in 3.2.3            http://commons.wikimedia.org/wiki/File:Leucine.png
Alanine     Arginine   Leucine




Yeah, that bit…
Drawn slightly differently you can see the bit that is
always the same and the R Group.
The R group is like x in an equation. It is a variable
that stands in for a bunch of different side chains




                                           http://commons.wikimedia.org/wiki/File:AminoAcidball.svg
The amine
group (NH2)
                                    The acid
                                    group (COOH)




      Look out for this structure
Hmmm… an amine group and an acid
group…

What shall we
call this class
of molecule?




                        http://commons.wikimedia.org/wiki/File:Filos_tercer_logo.JPG
The amine and acid groups
could be at opposite ends, the
R could be on top, bottom or
side depending on orientation.
Or it could be represented differently:
Don’t freak out, you don’t need to
know them all, just the general formula
                                 http://commons.wikimedia.org/wiki/File:Amino_Acids.svg
General structural formula for a
fatty* acid                                                               O
            H3C              (CH2)n                        C
             Chain (or ring) of carbon                                    OH
              and hydrogen atoms
                                                      Carboxylic group




     *I prefer “big boned”           http://www.eufic.org/article/pt/nutricao/gorduras/expid/23/
3.2.3 List three examples each of monosaccharides, disaccharides and polysaccharides

Monosaccharide #1
                                            Glucose has the formula C6H12O6
                                             It forms a hexagonal ring (hexose)

                                                   Glucose is the form
                                                   of sugar that fuels
                                                       respiration
                                                               Glucose forms the
                                                               base unit for many
                                                                    polymers

                                             5 of the carbons form
                                              corners on the ring
                                              with the 6th corner
                                                taken by oxygen
                                                      http://commons.wikimedia.org/wiki/File:Glucose_crystal.jpg
Monosaccharide #2                                            Galactose is also a
                                                                 hexose sugar
           Spot the difference                                 It has the same
                                                              formula C6H12O6
                                                              but is less sweet



                                                                Most commonly
                                                                 found in milk,
                                                                but also found
    Galactose                    Glucose                           in cereals




                                     http://commons.wikimedia.org/wiki/File:Galactose-3D-balls.png
                                 http://commons.wikimedia.org/wiki/File:Alpha-D-glucose-3D-balls.png
Monosaccharide #3     Ribose is a pentose
                         sugar, it has a
                        pentagonal ring


                    It forms the backbone
                            of RNA


                     Deoxyribose differs as
                         shown in the
                    diagram, and forms the
                       backbone of DNA




                            Original owner of image unknown
Monosaccharide #4                          Fructose is another
You only need 3, this is a bonus!            pentose sugar




                                       Commonly found in
                                        fruits and honey


                                     It is the sweetest
                                    naturally occurring
                                        carbohydrate

                                       http://www.flickr.com/photos/max_westby/4045923/
                                     http://commons.wikimedia.org/wiki/File:Red_Apple.jpg
                                     http://commons.wikimedia.org/wiki/File:3dfructose.png
Disaccharide #1
(Literally “two sugars”)               Lactose (C12H22O11) is most
                                        commonly found in milk


                                                    The two subunits that
                                                     make up lactose are
                                                    glucose and galactose,
                                                      our friends from a
                                                     couple of slides ago.




                                                http://www.flickr.com/photos/vermininc/2764742483/
                           http://commons.wikimedia.org/wiki/File:Alpha-lactose-from-xtal-3D-balls.png
Disaccharide #2
                  Sucrose (C12H22O11) is also
                    known as table sugar


                        The two subunits that
                   The two monosaccharides
                      that make itsucrose are
                         make up up are
                        glucose and fructose.
                     glucose and fructose




                         http://commons.wikimedia.org/wiki/File:Sucrose.gif
                      http://www.flickr.com/photos/carowallis1/4388310394/
Disaccharide #3
                                            Maltose (C12H22O11) is a dimer
                                                     of glucose




   Gosh! Isn’t it sweet?! The two glucose
      molecules are holding hands.




                                                 http://commons.wikimedia.org/wiki/File:Maltose_syrup.jpg
                                              http://commons.wikimedia.org/wiki/File:Maltose_Haworth.svg
Polysaccharide #1
                                       Glycogen (C6H10O5)n is a
                                      polymer made of repeating
                                           glucose subunits

  It is not just a simple chain, it
        branches many times




                                      http://en.wikipedia.org/wiki/File:Glycogen_spacefilling_model.jpg
Polysaccharide #2                                   Amylose is a form
                                                     of starch, and is
                                                    made of repeating
                                                      glucose units




          Typically an amylose molecule
         is made up of 300-3000 glucose
          units, but it can be many more


                                    http://www.flickr.com/photos/caroslines/5534432762/
                                    http://commons.wikimedia.org/wiki/File:Amylose3.svg
Polysaccharide #3                Cellulose (C6H10O5)n is a
                                  linear molecule made
                                   of repeating glucose
                                          units
  Multiple hydrogen bonds form
   between adjacent strands.
   Making strong microfibrils.




                                           http://www.flickr.com/photos/caroslines/5534432762/
                                 http://en.wikipedia.org/wiki/File:Cellulose_spacefilling_model.jpg
3.2.4 State one function of glucose, lactose and glycogen in animals, and of fructose, sucrose
and cellulose in plants
       After expending 10 slides on a “List” question, let’s answer this one in a table!

                           Carb.            Molecule                         Function
                       Glucose            Monosaccharide          Used in respiration to
                                                                  produce ATP
                       Lactose            Disaccharide            Energy source in milk
                       Glycogen           Polysaccharide          Short term energy storage
                                                                  in liver and muscles

                       Fructose           Monosaccharide          Energy source in fruits and
                                                                  honey
                       Sucrose            Disaccharide            Convenient form for
                                                                  transferring energy
                                                                  around plants
                       Cellulose          Polysaccharide          Structural component of
                                                                  the cell wall

                                                                  http://www.flickr.com/photos/pasma/575078217/
                                                         http://www.flickr.com/photos/93387088@N04/8527309132/
3.2.5 Outline the role of condensation and hydrolysis in the relationships between
monosaccharides, disaccharides and polysaccharides; between fatty acids and triglycerides; and
between amino acids and polypeptides.




           Two ways to help you remember what happens in
           condensation:
           • Water (H20) is formed, like condensation on a glass
           • The former separate molecules have “squished up“
             together and are “condensed”
                                                           http://www.flickr.com/photos/good_day/315807334/
Hydrolysis
(water- splitting)

H2O is split in the process
of breaking apart a
polymer, typically one H
will bind to one of the
resultant molecules and
OH will bind to the other.
 Condensation
                                      reactions joining two
                                      glucose molecules to
                                      make maltose + water



The bond between saccharide units is called a glycosidic bond




                                      Original source of glucose maltose image unknown
You might see the term “dehydration reaction”
mentioned interchangeably with condensation reaction.

 Technically a dehydration reaction is when the water
    molecule has come from one of the reactants.

Whereas for a condensation reaction, part of each water
       molecule has come from each reactant.

 In the case of the previous slide, OH from one glucose
                  and H from the other.




                                  http://www.flickr.com/photos/zachd1_618/5738829330/
 Hydrolysis reactions
                          break starch down into
                            maltose molecules.

                                  Amylase in saliva
                                     at work
                                               See 6.1




Hydrolysis of lactose
 into galactose and
     glucose 

  Lactase at work
       See 3.6.5
                        http://commons.wikimedia.org/wiki/File:Lactose_hydrolysis.svg
                        http://commons.wikimedia.org/wiki/File:Amylase_reaction.png
Condensation
reactions between
amino acids lead
to dipeptide
molecules

Keep adding
amino acids and
you end up with a
polypeptide.

These reactions
are catalysed by
ribosomes in cells
See 3.5 and HL 7.4
                     http://commons.wikimedia.org/wiki/File:Peptidformationball.svg
Hydrolysis reactions
       break peptides down
       into their constituent
          amino acids. The
         body can then use
       those amino acids to
             make new
         peptides/proteins.

         Pepsin is an enzyme
         in the stomach that
         hydrolyses proteins
                         See 6.1




http://commons.wikimedia.org/wiki/File:Amino_acid4.png
Condensation reaction between glycerol and fatty acids




Glycerol     Three Fatty Acids              Triglyceride

 Hydrolysis is the reverse of this                         3H2O
  process, catalysed by lipase

             Lipids are glycerol combined with 1, 2 or 3 fatty
                  acids, therefore triglycerides are lipids
3.2.6 State 3 functions of lipids




     1) Insulation:
    look how round
      this seal is!
                                    http://www.flickr.com/photos/ucumari/2585053774/
2) Energy Storage:
the fat on this bacon
 was a piggy’s bank
      of energy
      http://www.flickr.com/photos/johnnystiletto/5411371373/
3) Cell membranes: look all those
       lovely phospholipids!




Take a moment to review 2.4 cell membranes and
       name all of the bits in this diagram

                          http://commons.wikimedia.org/wiki/File:Cell_membrane_detailed_diagram_blank.s
                                                                                                    vg
3.2.7 Compare the use of carbohydrates and lipids in energy storage



                                    Energy Ratio by mass
                                           Fat : Carbs : Protein
                                              2:1:1
                                                    So it makes the most sense for
                                                  animals to store long term energy
                                                  reserves as fat. The same amount
                                                  of energy stored as carbohydrates
     The energy stored in lipids is                   would mass twice as much.
    primarily in the fatty acid side
   chains, so triglycerides are used
    for energy storage rather than
   diglycerides or monoglycerides
                                                           http://www.flickr.com/photos/markscott/162791929/
Glycogen is the medium-term energy storage molecule in animals. It
 is stored in the liver and muscles. The energy stored in glycogen is
         more readily available than the energy stored in fat.

Glucose in the bloodstream is for immediate use and will either be
  used in respiration to yield ATP or converted to glycogen or fat

An analogy:                           Wallet
                                      (Glycogen)
                                                         easy to get to,
                                                   would be too big if you put
                                                       in all your money

           You are
         paid in cash
                    (Glucose)
                                                          Bank
                                                          (Fat)
                                           Can put lots of money here, more
                                             of a hassle to get it back out
          Spend it!
           (Respiration)
Unlike most animals,
  most plants do not
need to move, so they
 store their energy as
    carbohydrates.

      Especially plants with storage
         roots, shoots or tubers

                    http://www.flickr.com/photos/87106931@N00/8359169832/
Seeds are the exception,
               they need to disperse to
                spread the species. So
              they use lipids for energy
                      storage.

Thus sunflower
oil, canola oil etc
Further information:




                       Three of the best sites for
                       IB-specific Biology
                       information. The top link
                       takes you to the PPT by
                       Stephen Taylor

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IB Biology Core 3.2: Carbohydrates Lipids and Proteins

  • 1. IB Biology 3 Chemistry of Life 3.2 Carbohydrates, Lipids and Proteins All syllabus statements ©IBO 2007 All images CC or public domain or link to original material. Jason de Nys http://commons.wikimedia.org/wiki/File:Burger_King_Angus_Bacon_%26_Cheese_Steak_Burger.jpg
  • 3. 3.2.1 Distinguish between organic and inorganic compounds Organic molecules are based on carbon and are in living things. Inorganic compounds are everything else plus carbonates (e.g. HCO3⁻ and CaCO3), carbon dioxide (CO2 )and carbon monoxide (CO). http://commons.wikimedia.org/wiki/File:GraphiteUSGOV.jpg
  • 4. 3.2.2 Identify amino acids, glucose*, ribose* and fatty acids from diagrams showing their structure Alanine Arginine Leucine Here are three of the twenty-one amino acids found in eukaryotes. Identify what parts of their structures are identical. http://commons.wikimedia.org/wiki/File:Alanine.png http://commons.wikimedia.org/wiki/File:Arginine.png *glucose and ribose shown in 3.2.3 http://commons.wikimedia.org/wiki/File:Leucine.png
  • 5. Alanine Arginine Leucine Yeah, that bit…
  • 6. Drawn slightly differently you can see the bit that is always the same and the R Group. The R group is like x in an equation. It is a variable that stands in for a bunch of different side chains http://commons.wikimedia.org/wiki/File:AminoAcidball.svg
  • 7. The amine group (NH2) The acid group (COOH) Look out for this structure
  • 8. Hmmm… an amine group and an acid group… What shall we call this class of molecule? http://commons.wikimedia.org/wiki/File:Filos_tercer_logo.JPG
  • 9. The amine and acid groups could be at opposite ends, the R could be on top, bottom or side depending on orientation.
  • 10. Or it could be represented differently:
  • 11. Don’t freak out, you don’t need to know them all, just the general formula http://commons.wikimedia.org/wiki/File:Amino_Acids.svg
  • 12. General structural formula for a fatty* acid O H3C (CH2)n C Chain (or ring) of carbon OH and hydrogen atoms Carboxylic group *I prefer “big boned” http://www.eufic.org/article/pt/nutricao/gorduras/expid/23/
  • 13. 3.2.3 List three examples each of monosaccharides, disaccharides and polysaccharides Monosaccharide #1 Glucose has the formula C6H12O6 It forms a hexagonal ring (hexose) Glucose is the form of sugar that fuels respiration Glucose forms the base unit for many polymers 5 of the carbons form corners on the ring with the 6th corner taken by oxygen http://commons.wikimedia.org/wiki/File:Glucose_crystal.jpg
  • 14. Monosaccharide #2 Galactose is also a hexose sugar Spot the difference It has the same formula C6H12O6 but is less sweet Most commonly found in milk, but also found Galactose Glucose in cereals http://commons.wikimedia.org/wiki/File:Galactose-3D-balls.png http://commons.wikimedia.org/wiki/File:Alpha-D-glucose-3D-balls.png
  • 15. Monosaccharide #3 Ribose is a pentose sugar, it has a pentagonal ring It forms the backbone of RNA Deoxyribose differs as shown in the diagram, and forms the backbone of DNA Original owner of image unknown
  • 16. Monosaccharide #4 Fructose is another You only need 3, this is a bonus! pentose sugar Commonly found in fruits and honey It is the sweetest naturally occurring carbohydrate http://www.flickr.com/photos/max_westby/4045923/ http://commons.wikimedia.org/wiki/File:Red_Apple.jpg http://commons.wikimedia.org/wiki/File:3dfructose.png
  • 17. Disaccharide #1 (Literally “two sugars”) Lactose (C12H22O11) is most commonly found in milk The two subunits that make up lactose are glucose and galactose, our friends from a couple of slides ago. http://www.flickr.com/photos/vermininc/2764742483/ http://commons.wikimedia.org/wiki/File:Alpha-lactose-from-xtal-3D-balls.png
  • 18. Disaccharide #2 Sucrose (C12H22O11) is also known as table sugar The two subunits that The two monosaccharides that make itsucrose are make up up are glucose and fructose. glucose and fructose http://commons.wikimedia.org/wiki/File:Sucrose.gif http://www.flickr.com/photos/carowallis1/4388310394/
  • 19. Disaccharide #3 Maltose (C12H22O11) is a dimer of glucose Gosh! Isn’t it sweet?! The two glucose molecules are holding hands. http://commons.wikimedia.org/wiki/File:Maltose_syrup.jpg http://commons.wikimedia.org/wiki/File:Maltose_Haworth.svg
  • 20. Polysaccharide #1 Glycogen (C6H10O5)n is a polymer made of repeating glucose subunits It is not just a simple chain, it branches many times http://en.wikipedia.org/wiki/File:Glycogen_spacefilling_model.jpg
  • 21. Polysaccharide #2 Amylose is a form of starch, and is made of repeating glucose units Typically an amylose molecule is made up of 300-3000 glucose units, but it can be many more http://www.flickr.com/photos/caroslines/5534432762/ http://commons.wikimedia.org/wiki/File:Amylose3.svg
  • 22. Polysaccharide #3 Cellulose (C6H10O5)n is a linear molecule made of repeating glucose units Multiple hydrogen bonds form between adjacent strands. Making strong microfibrils. http://www.flickr.com/photos/caroslines/5534432762/ http://en.wikipedia.org/wiki/File:Cellulose_spacefilling_model.jpg
  • 23. 3.2.4 State one function of glucose, lactose and glycogen in animals, and of fructose, sucrose and cellulose in plants After expending 10 slides on a “List” question, let’s answer this one in a table! Carb. Molecule Function Glucose Monosaccharide Used in respiration to produce ATP Lactose Disaccharide Energy source in milk Glycogen Polysaccharide Short term energy storage in liver and muscles Fructose Monosaccharide Energy source in fruits and honey Sucrose Disaccharide Convenient form for transferring energy around plants Cellulose Polysaccharide Structural component of the cell wall http://www.flickr.com/photos/pasma/575078217/ http://www.flickr.com/photos/93387088@N04/8527309132/
  • 24. 3.2.5 Outline the role of condensation and hydrolysis in the relationships between monosaccharides, disaccharides and polysaccharides; between fatty acids and triglycerides; and between amino acids and polypeptides. Two ways to help you remember what happens in condensation: • Water (H20) is formed, like condensation on a glass • The former separate molecules have “squished up“ together and are “condensed” http://www.flickr.com/photos/good_day/315807334/
  • 25. Hydrolysis (water- splitting) H2O is split in the process of breaking apart a polymer, typically one H will bind to one of the resultant molecules and OH will bind to the other.
  • 26.  Condensation reactions joining two glucose molecules to make maltose + water The bond between saccharide units is called a glycosidic bond Original source of glucose maltose image unknown
  • 27. You might see the term “dehydration reaction” mentioned interchangeably with condensation reaction. Technically a dehydration reaction is when the water molecule has come from one of the reactants. Whereas for a condensation reaction, part of each water molecule has come from each reactant. In the case of the previous slide, OH from one glucose and H from the other. http://www.flickr.com/photos/zachd1_618/5738829330/
  • 28.  Hydrolysis reactions break starch down into maltose molecules. Amylase in saliva at work See 6.1 Hydrolysis of lactose into galactose and glucose  Lactase at work See 3.6.5 http://commons.wikimedia.org/wiki/File:Lactose_hydrolysis.svg http://commons.wikimedia.org/wiki/File:Amylase_reaction.png
  • 29. Condensation reactions between amino acids lead to dipeptide molecules Keep adding amino acids and you end up with a polypeptide. These reactions are catalysed by ribosomes in cells See 3.5 and HL 7.4 http://commons.wikimedia.org/wiki/File:Peptidformationball.svg
  • 30. Hydrolysis reactions break peptides down into their constituent amino acids. The body can then use those amino acids to make new peptides/proteins. Pepsin is an enzyme in the stomach that hydrolyses proteins See 6.1 http://commons.wikimedia.org/wiki/File:Amino_acid4.png
  • 31. Condensation reaction between glycerol and fatty acids Glycerol Three Fatty Acids Triglyceride Hydrolysis is the reverse of this 3H2O process, catalysed by lipase Lipids are glycerol combined with 1, 2 or 3 fatty acids, therefore triglycerides are lipids
  • 32. 3.2.6 State 3 functions of lipids 1) Insulation: look how round this seal is! http://www.flickr.com/photos/ucumari/2585053774/
  • 33. 2) Energy Storage: the fat on this bacon was a piggy’s bank of energy http://www.flickr.com/photos/johnnystiletto/5411371373/
  • 34. 3) Cell membranes: look all those lovely phospholipids! Take a moment to review 2.4 cell membranes and name all of the bits in this diagram http://commons.wikimedia.org/wiki/File:Cell_membrane_detailed_diagram_blank.s vg
  • 35. 3.2.7 Compare the use of carbohydrates and lipids in energy storage Energy Ratio by mass Fat : Carbs : Protein 2:1:1 So it makes the most sense for animals to store long term energy reserves as fat. The same amount of energy stored as carbohydrates The energy stored in lipids is would mass twice as much. primarily in the fatty acid side chains, so triglycerides are used for energy storage rather than diglycerides or monoglycerides http://www.flickr.com/photos/markscott/162791929/
  • 36. Glycogen is the medium-term energy storage molecule in animals. It is stored in the liver and muscles. The energy stored in glycogen is more readily available than the energy stored in fat. Glucose in the bloodstream is for immediate use and will either be used in respiration to yield ATP or converted to glycogen or fat An analogy: Wallet (Glycogen) easy to get to, would be too big if you put in all your money You are paid in cash (Glucose) Bank (Fat) Can put lots of money here, more of a hassle to get it back out Spend it! (Respiration)
  • 37. Unlike most animals, most plants do not need to move, so they store their energy as carbohydrates. Especially plants with storage roots, shoots or tubers http://www.flickr.com/photos/87106931@N00/8359169832/
  • 38. Seeds are the exception, they need to disperse to spread the species. So they use lipids for energy storage. Thus sunflower oil, canola oil etc
  • 39. Further information: Three of the best sites for IB-specific Biology information. The top link takes you to the PPT by Stephen Taylor