PLEASE DOWNLOAD TO SEE ANIMATION.
San Diego Research Ethics Consortium,
Salk Institute for Biological Studies.
Workshop, Parthenogenetic stem cell lines: Ethical considerations.
8 July 2011, La Jolla, CA
youtube link : https://www.youtube.com/watch?v=da69DB6dU58&lc=z13osnvyfnnryny2z22qh3y4rs2bd3h2d
Stem cells can be defined simply as cells which are not specialized in any specific tissue or organs.
In other words, stem cells have not differentiated into other cell types to form tissues and organs.
They are the base or foundational cells to develop into cells which specialized in certain functions.
Another distinguishing characteristics of stem cells is their ability to undergo division, giving rise to more stem cells.
The significance of stem cells in their application to the human body and human health boils down to the two important characteristics of differentiation and self-regeneration.
Imagine how powerful they can be if stems cells can be developed into heart cells, especially when someone’s heart is doomed to fail Or, for someone with damaged brain cells or nerve cells, wouldn’t it be extremely great news if stem cells can develop new brain cells or nerve cells for the person.
Indeed, the potential and possibilities of exploiting stem cells for medical science and health science are enormous.
Many untreatable diseases and ailments may in the near future become curable.
Stem cells are classified into various types based on their ability to undergo differentiation into different cell types.
In other words, their classification, and hence their name, is derived from their potential to develop into one, two or several other cell types.
In my presentation I’ll discuss the principals of formation the stem cell and its applications .
youtube link : https://www.youtube.com/watch?v=da69DB6dU58&lc=z13osnvyfnnryny2z22qh3y4rs2bd3h2d
Stem cells can be defined simply as cells which are not specialized in any specific tissue or organs.
In other words, stem cells have not differentiated into other cell types to form tissues and organs.
They are the base or foundational cells to develop into cells which specialized in certain functions.
Another distinguishing characteristics of stem cells is their ability to undergo division, giving rise to more stem cells.
The significance of stem cells in their application to the human body and human health boils down to the two important characteristics of differentiation and self-regeneration.
Imagine how powerful they can be if stems cells can be developed into heart cells, especially when someone’s heart is doomed to fail Or, for someone with damaged brain cells or nerve cells, wouldn’t it be extremely great news if stem cells can develop new brain cells or nerve cells for the person.
Indeed, the potential and possibilities of exploiting stem cells for medical science and health science are enormous.
Many untreatable diseases and ailments may in the near future become curable.
Stem cells are classified into various types based on their ability to undergo differentiation into different cell types.
In other words, their classification, and hence their name, is derived from their potential to develop into one, two or several other cell types.
In my presentation I’ll discuss the principals of formation the stem cell and its applications .
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
Embryo culture is a component of in vitro fertilisation where in resultant embryos are allowed to grow for some time in an artificial medium before being inserted into the uterus.
In placental mammals, the umbilical cord (also called the navel string, birth cord or funiculus umbilicalis) is a conduit between the developing embryo or fetus and the placenta. During prenatal development, the umbilical cord is physiologically and genetically part of the fetus and, (in humans), normally contains two arteries (the umbilical arteries) and one vein (the umbilical vein), buried within Wharton's jelly. The umbilical vein supplies the fetus with oxygenated, nutrient-rich blood from the placenta. Conversely, the fetal heart pumps deoxygenated, nutrient-depleted blood through the umbilical arteries back to the placenta.
The blood within the umbilical cord, known as cord blood, is a rich and readily available source of primitive, undifferentiated stem cells (of type CD34-positive and CD38-negative). These cord blood cells can be used for bone marrow transplant.
Some parents choose to have this blood diverted from the baby's umbilical blood transfer through early cord clamping and cutting, to freeze for long-term storage at a cord blood bank should the child ever require the cord blood stem cells (for example, to replace bone marrow destroyed when treating leukemia).
In the future, cord blood-derived embryonic-like stem cells (CBEs) may be banked and matched with other patients, much like blood and transplanted tissues. The use of CBEs could potentially eliminate the ethical difficulties associated with embryonic stem cells (ESCs).
INTRODUCTION
DEFINATION
GAMETES
STRUCTURE OF GAMETES
SPERM
OVUM
RECOGNITION OF EGG AND SPERM
CAPACITATION
ACROSOME REACTION
SPECIES-SPECIFIC RECOGNITION
GAMETE BINDING AND RECOGNITION
GAMETE FUSION
PREVENTION OF POLYSPERMY
ACTIVATION OF GAMETE METABOLISM
FUSION OF THE GENETIC MATERIAL
SIGNIFICANCE OF FERTILIZATION
CONCLUSIONS
REFERENCES
A stem cell is a "blank" cell that can give rise to multiple tissue types such as a skin, muscle, or nerve cell.
Under certain physiologic or experimental conditions, they can be induced to become tissue- or organ-specific cells with special functions.
Embryo culture is a component of in vitro fertilisation where in resultant embryos are allowed to grow for some time in an artificial medium before being inserted into the uterus.
In placental mammals, the umbilical cord (also called the navel string, birth cord or funiculus umbilicalis) is a conduit between the developing embryo or fetus and the placenta. During prenatal development, the umbilical cord is physiologically and genetically part of the fetus and, (in humans), normally contains two arteries (the umbilical arteries) and one vein (the umbilical vein), buried within Wharton's jelly. The umbilical vein supplies the fetus with oxygenated, nutrient-rich blood from the placenta. Conversely, the fetal heart pumps deoxygenated, nutrient-depleted blood through the umbilical arteries back to the placenta.
The blood within the umbilical cord, known as cord blood, is a rich and readily available source of primitive, undifferentiated stem cells (of type CD34-positive and CD38-negative). These cord blood cells can be used for bone marrow transplant.
Some parents choose to have this blood diverted from the baby's umbilical blood transfer through early cord clamping and cutting, to freeze for long-term storage at a cord blood bank should the child ever require the cord blood stem cells (for example, to replace bone marrow destroyed when treating leukemia).
In the future, cord blood-derived embryonic-like stem cells (CBEs) may be banked and matched with other patients, much like blood and transplanted tissues. The use of CBEs could potentially eliminate the ethical difficulties associated with embryonic stem cells (ESCs).
INTRODUCTION
DEFINATION
GAMETES
STRUCTURE OF GAMETES
SPERM
OVUM
RECOGNITION OF EGG AND SPERM
CAPACITATION
ACROSOME REACTION
SPECIES-SPECIFIC RECOGNITION
GAMETE BINDING AND RECOGNITION
GAMETE FUSION
PREVENTION OF POLYSPERMY
ACTIVATION OF GAMETE METABOLISM
FUSION OF THE GENETIC MATERIAL
SIGNIFICANCE OF FERTILIZATION
CONCLUSIONS
REFERENCES
In Vitro Analog of the Primitive Streak (ANIMATED)Nikolay Turovets
PLEASE DOWNLOAD TO SEE ANIMATION.
In vitro analog of the primitive streak: efficient derivation of highly enriched populations of hepatocytes from various types of human pluripotent stem cells.
May, 2011
Radical Life Extension_Dr. Leon Peshkin_Dec 2023Levi Shapiro
Presentation for mHealth Israel by Dr. Leon Peshkin, Harvard Medical School, exploring research into Embryology and Longevity, emphasizing Germline and protein aggregates. Includes current overview, anti aging through history, Lifespan Expansion, ALEC: Animal Life Expectancy in Controls, Scalable Platform in Pharmacobiology, Embryos Cleanse Protein Aggregates, Lysosomal switch triggers proteostasis renewal, Xenopus, Rejuvenation Roadmap, Citizen Science Approach to Aging, Xenopus: Maternal Dowry Molecules, Human Disease Modeling
Reprogramming to pluripotency is possible from adult cells of different tissues and species through the ectopic expression of defined factors. The generated induced Pluripotent Stem Cells (iPSCs) are relevant for various purposes, including disease modeling, drug or toxicity screening and autologous cell therapy. Over the last few years, increased efforts are being made to improve the reprogramming techniques, the efficiency and quality of the generated iPSCs, as well as to identify the best cell source to be reprogrammed. Cells derived from fetal tissues, such as amniotic fluid, placenta and umbilical cord, offer distinct advantages in terms of reprogramming compared to adult somatic cells. Importantly, fetal cells are more primitive, easily achievable in sufficient numbers and are devoid of any ethical concern. They show great plasticity, high proliferation rate, low immunogenity and absence of teratoma formation. Therefore, they can be reprogrammed much faster and more efficiently than adult cells. Here, we provide a comprehensive overview of the advantages of reprogramming fetal sources in comparison to other commonly used cell types.
Comparative analysis of gene regulation in mouse rat and humanconstantina mylona
Which is the most suitable model mouse or rat even in the use of the latest gene editing tool-CRISPR/Cas 9 ?
Final Project presentaion on BSc Human Biology
Fertility Restoration after Cancer: Current and Future Therapies By Paul J. ...The Turek Clinics
Urologist and male fertility doctor for vasectomy and vasectomy reversal, sperm retrieval, testicular mapping, varicocele repair and ejaculatory duct repair, Dr. Paul Turek, speaks about Fertility Restoration after Cancer: Current and Future Therapies. Dr. Turek is director of The Turek Clinic. Located in San Francisco, California, The Turek Clinic provides world-class patient care with an essential holistic approach. (WARNING: Images in slides not appropriate for all audiences due to subject matter.)
Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
The Gram stain is a fundamental technique in microbiology used to classify bacteria based on their cell wall structure. It provides a quick and simple method to distinguish between Gram-positive and Gram-negative bacteria, which have different susceptibilities to antibiotics
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
Follow us on: Pinterest
Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Basavarajeeyam is an important text for ayurvedic physician belonging to andhra pradehs. It is a popular compendium in various parts of our country as well as in andhra pradesh. The content of the text was presented in sanskrit and telugu language (Bilingual). One of the most famous book in ayurvedic pharmaceutics and therapeutics. This book contains 25 chapters called as prakaranas. Many rasaoushadis were explained, pioneer of dhatu druti, nadi pareeksha, mutra pareeksha etc. Belongs to the period of 15-16 century. New diseases like upadamsha, phiranga rogas are explained.
- Video recording of this lecture in English language: https://youtu.be/kqbnxVAZs-0
- Video recording of this lecture in Arabic language: https://youtu.be/SINlygW1Mpc
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Adv. biopharm. APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMSAkankshaAshtankar
MIP 201T & MPH 202T
ADVANCED BIOPHARMACEUTICS & PHARMACOKINETICS : UNIT 5
APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMS By - AKANKSHA ASHTANKAR
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
263778731218 Abortion Clinic /Pills In Harare ,ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group of receptionists, nurses, and physicians have worked together as a teamof receptionists, nurses, and physicians have worked together as a team wwww.lisywomensclinic.co.za/
1. Nikolay Turovets, PhD Director of Research and Therapeutic Development How is Parthenogenesis Done? San Diego Research Ethics Consortium Salk Institute for Biological Studies Parthenogenetic stem cell lines: Ethical considerations 8 July 2011, La Jolla, CA parthenogenesis.eventbrite.com www.turovets.com
2. Parthenogenetic activation is a way to create pluripotent stem cells without disruption of the viable embryo Fertilization Parthenogenetic activation Ca 2+ time fertilization Ionomycin 5 min Ca 2+ time parthenogenetic activation ionomycin 6-DMAP or puromycin 4-5 hours 6-DMAP or puromycin Nikolay Turovets, PhD www.turovets.com
3. Parthenogenetic activation Parthenogenetic activation is a way to create pluripotent stem cells without disruption of the viable embryo Fertilization No life possible Embryonic stem cells Parthenogenetic stem cells Nikolay Turovets, PhD www.turovets.com
4. HLA heterozygous hpSC Immune-matching with donor HLA homozygous hpSC Immune-matching with people other than the donor Two types of human parthenogenetic stem cells (hpSC) Nikolay Turovets, PhD www.turovets.com Recipient Transplantation Recipients cells Recipient Donor Transplantation Donor cells
9. HLA-heterozygous hpSC lines are totally immune-matched with the oocyte donors Nikolay Turovets, PhD www.turovets.com
10. One HLA-homozygous hpSC line can be immune-matched with millions of people Nikolay Turovets, PhD www.turovets.com
11. hpSCs are patented and published by ISCO Nikolay Turovets, PhD www.turovets.com
12. hpSCs respond to well characterized differentiation signals and demonstrate appropriate gene expression dynamics and transitions Nikolay Turovets, PhD www.turovets.com
13. Hepatocytes derived from hpSC demonstrate appropriate functions in vitro and in vivo ICG test PAS test glycogen storage PROD assay/P450 activity in vitro in vivo Nikolay Turovets, PhD www.turovets.com
15. Differentiation capacity of hpSC: Cornea-like structures CK 18-19 DAPI ZO-1 DAPI Nikolay Turovets, PhD www.turovets.com
16. Major Collaborators Hepatocytes Cedars-Sinai Medical Center, LA Jeffrey Fair UC San Francisco Holger Willenbring Definitive endoderm ViaCyte/Novocell Ed Baedge Kevin D’Amour RPE UC Irvine Hans Keirstead Magdalene Seiler Methylation and global gene expression The Scripps Research Institute Jeanne Loring Louise Laurent Neurology University of Wurzburg, Germany Albrecht Muller Immunogenicity studies UC San Diego Ewa Carrier Matthias von Herrath University of Berlin, Germany Hans-Dieter Volk Genetic studies Genesis Genetics Institute Marcus Hughes Nikolay Turovets, PhD www.turovets.com
Editor's Notes
Results of these work were published in two papers of “Cloning and Stem Cells” Journal in this and last years.