1. Targeted Drug Delivery System LIPOSOMES

17. Methods of liposomes preparations Passive loading technique Active loading technique Mechanical dispersion methods Solvent dispersion methods Detergent removal methods

20. Physical Characterization Surface charge Free-flow electrophoresis Electrical surface potential and surface pH Zetapotential measurements & pH sensitive probes Percent of free drug/ percent capture Drug release Diffusion cell/ dialysis Parameter Characterization method Vesicle shape and surface morphology Mean vesicle size and size distribution Dynamic light scattering, zetasizer, Photon correlation spectroscopy, laser light scattering, gel permeation and gel exclusion Minicolumn centrifugation, ion-exchange chromatography, radiolabelling Transmission electron microscopy, Freeze-fracture electron microscopy

21. Phopholipid peroxidation UV absorbance, Iodometric and GLC Phospholipid hydrolysis, Cholesterol auto-oxidation HPLC and TLC Osmolarity Parameter Characterization method Phospholipid concentration Cholesterol concentration Cholesterol oxidase assay and HPLC Osmometer Barlett assay, stewart assay, HPLC Chemical Characterization

22. Biological Characterization Animal toxicity Monitoring survival rates, histology and pathology Parameter Characterization method Sterility Pyrogenicity Limulus Amebocyte Lysate (LAL) test Aerobic or anaerobic cultures

28. Pseudomonas infection, aeruginosa Pulmonary delivery Tobramycin Asthma Pulmonary delivery Pentoxyfylline Pain muscle condition Ocular delivery Ketoprofen Diabetic mellitus Oral, Ocular, Pulmonary and Transdermal delivery Insulin Mycotic infection Oral delivery Amphotericin-B Targeted Diseases Route of administration Drug

29. Cancer Transdermal Methotrexate Meningococal, staphylococcal Pulmonary delivery Penicillin G Glucoma, Conjectivitis Ocular delivery Adrenaline Rheumatoid arthritis Oral delivery Ibuprofen ulcer on mucous surface with pain Transdermal Benzocain Asthma Pulmonary delivery Salbutamol Targeted Diseases Route of administration Drug

30. Britannia Pharm, UK Expanding lung diseases in babies Dry protein free powder of DPPC-PG ALECTM The liposome company, USA Systemic inflammatory diseases Prostaglandin-E1 VENTUSTM SEQUUS, USA fungal infections, Leishmaniasis Amphotericin-B AmphotecTM NeXstar, USA Kaposi’s sarcoma, breast & lung cancer Daunorubicin DaunoXomeTM SEQUUS, USA Kaposi’s sarcoma Doxorubicin DoxilTM or CaelyxTM Company Target diseases Drug used Marketed product

31. Swiss serum & vaccine institute, Switzerland Hepatitis A Inactivated hepatitis-A Virions Epaxal –Berna Vaccine Vineland lab, USA Chicken pox Killed avian retrovirus Avian retrovirus vaccine Novavax, USA Smallpox Smallpox vaccine Novasome® Skye Pharm, USA Cancer therapy Cytarabine Depocyt Ozone, USA Asthma Terbutaline sulphate Topex-Br Company Target diseases Drug used Marketed product

35. Any question...

36. Thank You