1. Hemoglobin and other heme-containing proteins are broken down, releasing iron and producing bilirubin, which is conjugated in the liver and excreted in bile and feces.
2. Heme synthesis takes place in the liver and bone marrow and is regulated by negative feedback inhibition by heme.
3. Issues with heme metabolism can cause porphyrias or jaundice in newborns from immature bilirubin conjugation enzymes.
simple diagrammatic presentation of heme catabolism. highlighted the steps with explanation. Definition , causes, clinical features and biochemical investigation of various types of jaundice is explained in detail. congenital jaundice is included.
rft is described in detail . function of kidney, objectives of doing the test. the various test available for assessing the renal function with clinical interpretation is available.
Describes the plasma membrane in detail, explains the each major component with its functions.
Transport mechanism across the cell is covered with detailed explanation with examples.
by Dr. N.Sivaranjani, MD
A comprehensive presentation on Hemoglobin chemistry for medical ,dental ,biotechnology ,Life sciences ,& pharmacology students. Presentation includes structure & functions of a normal hemoglobin molecule.Bohr's effect along with allosteric modulators of hemoglobin for oxygen transport are illustrated.Molecular changes ,types,diagnosis, Management & inheritance of Sickle cell anemia is described .Types , mutations involved ,diagnosis ,inhertance & Management of Thalassemia disease is presented here . Presentation also involves other hemoglobinopathies Hb C/D/E /Lepore/Wyane etc.Changes in oxygen carrying capacity of hemoglobin after formation of Carboxy Hemoglobin is illustrated . Formation of Meth-Hb in vivo & in vitro is described along with its genetic & diagnostic aspects.Unstable variants & chronic Heinz body anemia are described briefly .Text is supported by Google images.
explains the breakdown of purine. source and excretion of purine is explained. hyperuricemia and hypouricemia is discussed. types of Gout, clinical features and treatment is included.
Enzyme inhibition is explained with its kinetics, animations showing mechanism of inhibitors action, examples of inhibitors are explained in detail with Enzyme inhibited.
by Dr. N. Sivaranjani, MD
Nearly all water in the world contains contaminants, even in the absence of nearby pollution-causing activities
Many dissolved minerals, carbon compounds, and microbes find their way into drinking water as it comes in contact with air and soil
When pollutant and contaminant levels in drinking water are high, they may affect household routines and be detrimental to human health
The only way to ensure that your water supply is safe is to have a periodic laboratory water quality analysis done on your drinking water. Hach India is the leading provider of high end water quality analysis equipment in india
Liver function tests and interpretation is a very important topic for students of medical and allied fields. It is essential for efficient practice of clinical and laboratory medicine.
Disorders of liver and kidney, Nitrogen metabolism.pdfshinycthomas
Disorders of liver and kidney – Jaundice, fatty liver, normal and abnormal functions of liver and kidney. Inulin and urea clearance.
Abnormalities of nitrogen metabolism
This is a lecture note for 5th semester MBBS students. Lecture notes on hepatology, liver disease, alcoholic liver disease, alcohol-related liver disease, portal hypertension, and hepatic encephalopathy. Introduction to hepatic encephalopathy, causes, differentials, approach, and management of hepatic encephalopathy .
This content is suitable for medical technologists/technicians/lab assistants/scientists writing the SMLTSA board exam. The content is also suitable for biomedical technology students and people also interested in learning about the liver. This chapter describes the liver and interpretation of the liver function tests. Please note that these notes are a collection I used to study for my board exam and train others who got distinctions using these.
Disclaimer: Credit goes to those who wrote the notes and the examiners of each exam question. Please use only as a reference guide and use your prescribed textbook for the latest and most accurate notes and ranges. The material here is not referenced as it is a collection of pieces of study notes from multiple people, and thus will not be held viable for any misinterpretations. Please use at your own discretion.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
2. Objectives
• Haem synthesis
• Regulation of haem synthesis
• Catabolism of haem
• Regulation of haem catabolism
• Clinical importance of haem metabolism
3. Haem
Porphyrin+Fe
Some important human
haemoproteins are;
haemoglobin, myoglodin,cytochromes,
catalase, tryptophan pyrrolase
In plants Porphyrin+Zinc- photosynthetic
pigment chlorophyll
N
N
N
N
CH3 HC
CH3
S CH2
CH3
CH S CH2
CH3
CH2
CH2
COO-
CH3
H3C
CH2CH2
-
OOC
protein
protein
Fe
4. Haem Synthesis
Takes place in the bone marrow and liver
Starts with condensation of glycine and
succinyl CoA in the mitochondria
Condensation reaction is catalysed by a-
aminolevulinate synthase (ALA synthase)
In the liver its ALA synthase I and in the
bone marrow its ALA synthase II.
Co-factor needed for this reaction is
pyridoxal phosphate
This reaction is the rate limiting step of
haem synthesis
OOC CH2 CH2 C S-CoA
O
+
OOC CH2 NH3
+
OOC CH2 CH2 C
O
CH2 NH3
+
CO2CoA-SH
H+succinyl-CoA glycine
-aminolevulinate (ALA)
-Aminolevulinic
AcidSynthase
6. ① It is regulated by repression mechanism. Heme
inhibits the synthesis of ALA synthesis by acting as a
corepressor. The feedback regulatory effect is a typical
example of end-product inhibition.
1. ALA synthase
Major site of regulation is at the level of ALA synthase.
Regulation of heme synthesis
7. ② ALA synthase is also allosterically inhibited by
haematin.
When there is excess of free heme without globin
chains to bind with, the Fe++ is oxidized to Fe+++ forming
hematin. Haematin will inhibit ALA synthase to prevent
excessive unwanted production of haem.
Haematin will also inhibit the translocation of ALA synthase
from the cytoplasm into the mitochondria where its substrate,
succinyl CoA is formed. thus heme synthesis is inhibited till
there are sufficient globin chains to bind with.
8. ③ Lack of Vit B6 will decrease the synthesis of ALA.
Drugs like INH (isonicotinic acid hydrazide) that decrease
the availability of pyridoxal phosphate may also affect
heme synthesis.
4. Drugs that induce cytochrome P450 eg barbiturates
griseofulvin induce ALAS 1 synthesis. In patients with
porphyria these drugs can precipitate attacks
9. 2. Haem synthesis may be inhibited by heavy metals.
the steps catalyzed by ALA dehydratase and
ferrochelatase are inhibited by lead.
3. erythropoietin, EPO
The kidneys also secrete a hormone called erythropoietin.
The function of erythropoietin is to stimulate the production
of red blood cells. The kidney produces 85~95% of the body's
erythropoietin so when the kidney is damaged (kidney disease or
failure), not enough erythropoietin is produced to maintain
normal red blood cell levels. This leads to anemia.
10. Clinical Correlates
(Porphyria)
• Rare genetic diseases in which
activity of one of the enzymes
involved in heme synthesis is
decreased (e.g., PBG Synthase,
Porphobilinogen Deaminase,
etc…)
• Usually inherited in autosomal
dominant except congenital
erythropoeitic porphyria
• Symptoms depend on enzyme
affected, organ involved
• Symptoms are usually acute or
cutaneous
• Acute symptoms usually involves
enzymes before
uroporphobilinogen
(accumulation of ALA, PBG).
Includes abdominal pains and
neuropsychiatric symptoms
• Cutaneous symptoms results
from enzyme deficiencies from
uroporphobilinogen. Substrates
accumulate in skin leading to
oxidized products.
Photosensitvity –skin damage
13. • From family pedigree
• History of abdominal
pain+neuropsychiatric symptoms
• Photosensitive skin rashes
• Urine which changes colour on
exposure to sunlight (red)
• medical support for vomiting and
pain
• hemin, decreases ALA synthase
synthesis
• Consumption of large amounts of
carbohydrates
• avoidance of sunlight and
precipitating drugs, factors
• Avoid drugs that induce
cytochrome P450 eg barbiturates
14. Haem catabolism
Cytochrome has high turnover rate
Red blood cells have life span of 60-120 days
When haemoproteins are destroyed they yield haem
70% of haem from red blood cells
Haem is eventually converted to bilirubin for excretion
15. Extravascular Pathway for RBC Destruction
(Liver, Bone marrow,
& Spleen)
Haemoglobin
Globin
Amino acids
Amino acid pool
Haem Bilirubin
Fe2+
Excreted
Phagocytosis & Lysis
16. Haem degradation
• Takes place in the ER of
reticuloendothelial system of
spleen,liver,bone marrow
• CO monoxide produced is exhaled
(can be used to quantify haem
turnover rate)
• In birds and amphibians the green
biliverdin is excreted
• Bilirubin is sparingly soluble and must
be transported bound to albumin
• Bilirubin is transported to the liver for
elimination
17. • Bilirubin is taken up in the
liver by carrier-mediate
transport into parenchymal
cells
• In the cells bilirubin binds to
Ligandin and protein Y
• Conjugation of bilirubin to
make it polar is achieved by
addition of mainly
glucuronide. Others include
xylose, sulfate and ribose
• Conjugation with
glucuronide is catalysed by
glucuronyltransferase
(glucuronyl-UDP)
• Two glucuronic acid is
conjugated to bilirubin
(bilirubin diglucuronide)
• Conjugated bilirubin is
secreted into the bile by an
active transport mediated
by multidrug resistance like
protein 2 (MRP-2)
18. • Bile gets secreted into the intestine
• At the terminal ileumand large intestine
bacterial glucuronidases remove the
glucuronides to form
• The free bilirubin is reduced in the intestine
to the colourless urobilinogen
• Urobilinogen/stercobilinogen is oxidized to
the coloured urobilin/stercobilin (excreted in
feaces- imparts red brown colour to stool)
• Some of the urobilinogen is taken up by the
blood back to the liver to be re-excreted back
into the bile (enterohepatic urobilinogen
cycle)
• Urobilinogen excreted by the liver. Oxidized
to urobilin (gives urine its unique straw
colour
19. Hyperbilirubinaemia
• Increases levels of
bilirubin (conjugated or
unconjugated)
• Could be due to increased
bilirubin production or
decreased bilirubin
secretion
• Classified as pre hepatic,
intra-hepatic and post
hepatic
20. Neonatal Jaundice
• Common, particularly in premature infants
• Transient (resolves in the first 10 days)
• Due to immaturity of the enzymes involved in bilirubin conjugation
• High levels of unconjugated bilirubin are toxic to the newborn – due to its
hydrophobicity it can cross the blood-brain barrier and cause a type of mental
retardation known as kernicterus
• If bilirubin levels are judged to be too high, then phototherapy with UV light is used to
convert it to a water soluble, non-toxic form
• If necessary, exchange blood transfusion is used to remove excess bilirubin
• Phenobarbital is oftentimes administered to Mom prior to an induced labor of a
premature infant – crosses the placenta and induces the synthesis of UDP glucuronyl
transferase
• Jaundice within the first 24 hrs of life or which takes longer then 10 days to resolve is
usually pathological and needs to be further investigated