This document summarizes features of the Prism graphing software for customizing graphs. It discusses how to customize various graph types like scatter plots, bar charts, and box plots by changing axes, colors, labels, and more. It also covers exporting graphs, cloning graphs to create new figures easily, and using templates to store common graph types and analyses. Upcoming seminars on curve fitting and statistical applications in Prism are also listed.

1. 3/25/2010
1
Customizing Your Graphs in PrismCustomizing Your Graphs in Prism
Presented by: Jeff Skinner, M.S.
Biostatistics Specialist
Bioinformatics and Computational Biosciences Branch
National Institute of Allergy and Infectious Diseases
Office of Cyber Infrastructure and Computational Biology
Basic Biostatistics * Curve Fitting * Graphing

2. 3/25/2010
2
NIAID Site License
http://graphpad.com/paasl/index.cfm?sitecode=NIHNIAID
http://bioinformatics.niaid.nih.gov/
Prism Training and SupportPrism Training and Support
ScienceApps@niaid.nih.gov

3. 3/25/2010
3
Outline
• Overview of Prism software features
• Scatter Plot• Scatter Plot
– Axis limits, tick intervals, additional tick lines, subscripted text
– Color schemes, color choices, format individual points, floating notes
• Interleaved Bar Chart
– Axis breaks, format tick labels, special characters
• Box and Whiskers Plot
– Duplicate Graph Prism Layout files Overlay figuresDuplicate Graph, Prism Layout files, Overlay figures
• Exporting Graphs
– Copy‐and‐paste vs. Export graph options
• Prism Magic feature, Clone graphs, Prism Templates
Overview
• Five types of linked data
tables and graphs
– XY, Column, Grouped,
Contingency and Survival
• Importing data
– Copy‐and‐paste from Excel
– Prism Import WizardPrism Import Wizard
• Project organization
– Data Tables, Info files, Results,
Graphs and Layouts

4. 3/25/2010
4
Scatter Plot (XY)
• Format title for axes
and graph
• Change range and tick
intervals for axes
• Format symbol for
one point or one data
set
• Label point with row
title
• Add floating notes
• Create additional ticks
with labels
Interleaved Bar Chart (Grouped)
• Draw customized error
bars from raw data
• Format bars and lines
for all data sets
• Create axis breaks
• Format tick labels
• Insert special characters

5. 3/25/2010
5
Box and Whiskers Plot (Column)
• Change box andChange box and
whiskers type
• Duplicate family
• Create layouts
• Equalize and arrange
graphs on layouts
Exporting Graphs
• Send to MS Word
or MS PowerPoint
• Export graphs for
publication

6. 3/25/2010
6
Other Cool Features
• Clone a graph :C o e a g ap :
– Prism creates a copy of that graph along with linked data
tables, info sheets and analyses
– All you have to do is replace the data to create the new figure.
• Create a template
– Similar to “clone graph” feature, but allows user to create aSimilar to clone graph feature, but allows user to create a
menu of stored graphs and analyses
• Magic tool: Make graphs look consistent
Upcoming Seminars
• Curve Fitting in PRISM
– April 1st at 10 AM, Building 50, Room 1227/1233
• Statistical Applications in PRISM
– April 8th at 10 AM, Building 50, Room 1227/1233
• http://collab.niaid.nih.gov/sites/research/SIG/
Bioinformatics/seminars.aspx