Glycolysis is the metabolic pathway that converts glucose into pyruvate, generating a small amount of ATP. It occurs in the cytosol of cells and has three phases: energy investment, splitting of molecules, and energy generation. Glycolysis is regulated by three key enzymes - hexokinase, phosphofructokinase, and pyruvate kinase. It yields two ATP per glucose under anaerobic conditions when pyruvate is reduced to lactate, and eight ATP when pyruvate enters the citric acid cycle under aerobic conditions.
Pentose phosphate pathway is also called Hexose monophosphate pathway/ HMP shunt/ Phosphogluconate pathway.
It is an alternative route for the metabolism of glucose.
It is more complex pathway than glycolysis.
It is more anabolic in nature.
It takesplace in cytosol.
The tissues such as liver, adipose tissue, adrenal gland, erythrocytes,testes and lactating mammary gland are highly active in HMP shunt.
It concern with the biosynthesis of NADPH and pentoses.
Pentose phosphate pathway is also called Hexose monophosphate pathway/ HMP shunt/ Phosphogluconate pathway.
It is an alternative route for the metabolism of glucose.
It is more complex pathway than glycolysis.
It is more anabolic in nature.
It takesplace in cytosol.
The tissues such as liver, adipose tissue, adrenal gland, erythrocytes,testes and lactating mammary gland are highly active in HMP shunt.
It concern with the biosynthesis of NADPH and pentoses.
Glycolysis (from glycose, an older term for glucose + -lysis degradation) is the metabolic pathway that converts glucose C6H12O6, into pyruvate, CH3COCOO− + H+. The free energy released in this process is used to form the high-energy molecules ATP (adenosine triphosphate) and NADH (reduced nicotinamide adenine ...
Glycogenolysis, process by which glycogen, the primary carbohydrate stored in the liver and muscle cells of animals, is broken down into glucose to provide immediate energy and to maintain blood glucose levels during fasting. These slides will provide you detail explanation of Glycogenolysis.
Substrate level phosphorylation and it's mechanism || Biochemistry || B Pharmacy || Project || slideshare || biology || chemistry
*images use in this ppt is only for educational purpose
In this presentation, i tell about substrate level phosphorylation
Phosphorylation involves the transfer of phosphate
group from one compound to other.
➢ Substrate level phosphorylation is a direct
phosphorylation of ADP with a phosphatase group by
using the energy obtain from a coupled reaction.
➢ Occurs in cytoplasm ( glycolysis – due to aerobic and
anaerobic condition) and in mitochondrial matrix ( krebs
cycle – anaerobic condition)
Glycolysis (from glycose, an older term for glucose + -lysis degradation) is the metabolic pathway that converts glucose C6H12O6, into pyruvate, CH3COCOO− + H+. The free energy released in this process is used to form the high-energy molecules ATP (adenosine triphosphate) and NADH (reduced nicotinamide adenine ...
Glycogenolysis, process by which glycogen, the primary carbohydrate stored in the liver and muscle cells of animals, is broken down into glucose to provide immediate energy and to maintain blood glucose levels during fasting. These slides will provide you detail explanation of Glycogenolysis.
Substrate level phosphorylation and it's mechanism || Biochemistry || B Pharmacy || Project || slideshare || biology || chemistry
*images use in this ppt is only for educational purpose
In this presentation, i tell about substrate level phosphorylation
Phosphorylation involves the transfer of phosphate
group from one compound to other.
➢ Substrate level phosphorylation is a direct
phosphorylation of ADP with a phosphatase group by
using the energy obtain from a coupled reaction.
➢ Occurs in cytoplasm ( glycolysis – due to aerobic and
anaerobic condition) and in mitochondrial matrix ( krebs
cycle – anaerobic condition)
Carbohydrates are the sugars, starches and fibers found in fruits, grains, vegetables and milk products. Though often maligned in trendy diets, carbohydrates — one of the basic food groups — are important to a healthy diet.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
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2. Metabolism
Metabolism is “the entire set of enzyme-
catalyzed transformations of organic
molecules in living cells.
Two broad classes:
Catabolism & Anabolism
Catabolic Pathways:
Transform fuels into cellular energy
3. Requires inputs of energy to proceed.
Useful energy + small molecules
complex molecules.
Pathways that can be either anabolic or
catabolic, depending on the energy
conditions in the cell are referred to as
amphibolic pathways
Anabolic Pathways
4. Glycolysis occurs in almost every living cell.
It was the first metabolic sequence to be
studied.
This pathway is also called Embden-
Meyerhof pathway (E.M-Pathway).
It occurs in cytosol.
Glycolysis
5. Definition
Glycolysis is defined as the sequence of
reactions converting glucose to pyruvate or
lactate, with the production of ATP.
Salient features:
Takes place in all cells of the body.
The enzymes of this pathway are present in
the cytosomal fraction of the cell.
6. Glycolysis occurs in the absence of oxygen
(anaerobic) or in presence of oxygen
(aerobic).
Lactate is the end product under anaerobic
condition.
In aerobic condition, pyruvate is formed,
which is then oxidized to CO2 & H2O.
7. Glycolysis is a major pathway for ATP
synthesis in tissues lacking mitochondria,
erythrocytes, cornea, lens etc.
Glycolysis is very essential for brain which is
dependent on glucose for energy.
8. The glucose in brain has to undergo
glycolysis before it is oxidized to CO2 & H2O.
Glycolysis is a central metabolic pathway
with many of its intermediates providing
branch point to other pathways.
The intermediates of glycolysis are useful for
the synthesis of amino acids and fat.
9. Glucose entry into cells
Glucose transporter-4 (GluT4) transports
glucose from extracellular fluid to muscle
cells & adipocytes.
This is under the influence of Insulin.
In diabetes mellitus, insulin deficiency hinders
the entry of glucose into the peripheral cells.
GluT2 is the transporter in liver cell.
It is not under the control of insulin.
10. Reactions of Glycolysis
Divided into three distinct phases.
Energy investment phase or priming phase
Splitting phase
Energy generation phase.
11. Glucose obtained from
The diet through intestinal hydrolysis of
lactose, sucrose, glycogen, or starch is
brought into the hexose phosphate pool
through the action of hexokinase.
Free glucose is phosphorylated to glucose 6
phosphate by hexokinase
Energy investment phase
12. Hexokinase splits the ATP into ADP & Pi,
the Pi is added to the glucose.
Hexokinase Hexokinase is a key
glycolytic enzyme.
Glucose Glucose 6-Phosphate
Hexokinase or Glucokinase
ATP ADP
Mg+2
13. • Phosphorylated sugar molecules do not
readily penetrate cell membranes without
specific carriers, this commits glucose to
further metabolism in the cell.
• In all tissues, the phosphorylation of glucose
is catalyzed by hexokinase, one of the three
regulatory enzymes of glycolysis.
14. Hoxokinase and glucokinase
Hexokinase Glucokinase
Occurrence In all tissues Only in liver
Km Value 10-2 mmol/L 20 mmol/L
Affinity to substrate High Low
Specificity
Acts on glucose, fructose
and mannose
Acts only on glucose
Induction Not induced
Induced by insulin &
glucose
Function
Even when blood sugar
level is low, glucose is
utilized by body cells
Acts only when blood
glucose level is more then
100 mg/dl; then glucose is
taken up by the liver cells
for glycogen synthesis
15. Isomerization of Glucose 6-P
Glucose 6 P is a central molecule with a
variety of metabolic fates- glycolysis,
glycogenesis, gluconeogenesis and HMP
pathway.
The isomerization of Glucose 6-P (an aldose
sugar) to Fructose 6-P (a ketose sugar) is
catalyzed by phosphohexose isomerase
It requires Mg+2 ions.
16. The reaction is readily reversible, is NOT
a rate limiting or regulated step.
Glucose 6-Phosphate
Phosphohexose isomerase & Mg+2
Fructose 6-Phosphate
17. Phosphorylation of Fructose 6-P
• Fructose 6- phosphate is phosphorylated to Fructose
1, 6- bisphosphate by Phosphofructokinase (PFK)
• The PFK reaction is the rate-limiting step.
• It is controlled by the concentrations of the
substrates ATP & Fructose 6-P
Fructose 6P Fructose 1, 6-bisPhosphate
Phosphofructokinase
ATP ADP
Mg+2
18. Splitting Phase
• The six carbon Fructose 1, 6- bisphosphate is split
to 2 three carbon compounds.
• Glyceraldehyde 3- phosphate & Dihydroxy acetone
phosphate by the enzyme aldolase (Fructose 1, 6-
bisphosphate aldolase).
• The reaction is reversible is not subject to regulation.
Fructose 1,6-
bisphosphate
Glyceraldehyde 3-
Phosphate + DHAP
Aldolase
19. Isomerization of DHAP
• Phosphotriose isomerase catalyzes the reversible
interconversion of dihydroxyacetone phosphate &
glyceraldehyde 3-phosphate.
• Two molecules of glyceraldehyde 3-phosphate are
obtained from one molecule of glucose.
DHAP Glyceraldehyde 3-Phosphate
Phosphohexose isomerase
20. Oxidation of glyceraldehyde 3P
Glyceraldehyde 3-phosphate dehydrogenase
converts Glyceraldehyde 3-phosphate to 1,3-
bisphosphoglycerate.
This step is important as it is involved in the
formation of NADH +H+ & a high energy
compound 1,3- bisphosphoglycerate.
21. In aerobic condition, NADH passes through
the ET C and 6 ATP are synthesized by
oxidative phosphorylation.
Glyceraldehyde 3P 1,3-bisphosphoglycerate
Glyceraldehyde 3P-
dehydrogenase
NAD NADH+H+
Pi
22. Formation of ATP from 1,3-
bisphosphoglycerate & ADP
• The enzyme phosphoglycerate kinase acts on
1,3- bisphosphoglycerate resulting in the
synthesis of ATP and formation of 3-
phosphoglycerate.
1,3-bisphosphoglycerate 3P-glycerate
Phosphoglycerate kinase
ADP ATP
Mg+2
23. This step is a substrate-level phosphorylation
Production of a high-energy P is coupled to
the conversion of substrate to product, instead
of resulting from oxidative phosphorylation.
The energy will be used to make ATP in the
next reaction of glycolysis.
24. • The formation of ATP by P group transfer
from a substrate such as 1,3-
bisphosphoglycerate is referred to as a
substrate-level phosphorylation.
• Unlike most other kinases, this reaction is
reversible.
25. 3- Phosphoglycerate is converted to 2-
Phosphoglycerate by phosphoglycerate
mutase
This is isomerization reaction.
3-Phosphoglycerate 2P-glycerate
Phosphoglycerate mutase
26. The high energy compound PEP is generated
from 2- Phosphoglycerate by the enzyme
enolase.
This enzyme requires Mg+2 or Mn+2 and is
inhibited by fluoride.
2-Phoglycerate Phosphoenolpyruvate
Enolase
Mg+2
27. The enzyme pyruvate kinase catalyses the
transfer of high energy phosphate from PEP
to ADP, leading to the formation of ATP.
This step is also a substrate level
phosphorylation.
Phosphoenolpyruvate Pyruvate
Pyruvate kinase
ADP ATP
Mg+2
28. Glucose
Glucose 6-Phosphate
HK or GK
ATP
ADP
Mg+2
Phosphohexose isomerase
Fructose 6-Phosphate
Mg+2
Fructose 1, 6-bisphosphate
Phosphofructokinase
ATP
ADP
Mg+2
DHAP Glyceraldehyde 3-Phosphate
Aldolase
29. DHAP Glyceraldehyde 3-Phosphate
Phosphohexose isomerase
1,3-bisphosphoglycerate
Glyceraldehyde 3P-
dehydrogenase
NAD
NADH+H+
Pi
Iodoacetate,
Arsenate
3P-glycerate
Phosphoglycerate
kinase
ADP
ATP
Mg+2
2P-glycerate
Mutase
32. Regulation of glycolysis
Three regulatory enzymes:
Hexokinase & glucokinase
Phosphofructokinase
Pyruvate kinase
Catalysing the irreversible reactions
regulate glycolysis.
33. Hexokinase
Hexokinase is inhibited by glucose 6-
phosphate.
This enzyme prevents the accumulation of
glucose 6-phosphate due to product
inhibition.
35. Phosphofructokinase (PFK)
Phosphofructo kinase (PFK) is the most
important regulatory enzyme in glycolysis
PFK is an allosteric enzyme regulated by
allosteric effectors ATP, citrate & H+ ions (low
pH) are the most important allosteric
inhibitors.
Fructose 2 ,6-bisphosphate, ADP, AMP & Pi are
the allosteric activators.
36. Role of fructose 2,6-bisphosphate in glycolysis
Fructose-2,6-bisphosphate (F2,6-BP) is
considered to be the most important
regulatory factor (activator) for controlling
PFK & ultimately glycolysis in the liver.
F2,6-BP is synthesized from fructose 6-p by the
enzyme phosphofructokinase called PFK-2
(PFK-1 is the glycolytic enzyme)
37. F2,6-BP is hydrolysed by fructose 2,6 -
bisphosphatase.
The function of synthesis & degradation of F2,6-BP
is brought out by a single enzyme (same
polypeptide with two active sites) which is
referred to as bifunctional enzyme.
The activity of PFK-2 & fructose 2,6- bisphosphatase
is controlled by covalent modification which, in
turn, is regulated by c AMP.
38. Cyclic AMP brings about
dephosphorylation of the bifunctional
enzyme, resulting in inactivation of active
site responsible for the synthesis of F2,6-BP
but activation of the active site responsible
for the hydrolysis of F2,6-BP
39. Pyruvate kinase
PK Inhibited by ATP & activated by F1,6-BP.
Pyruvate kinase is active (a) in
dephosphorylated state & inactive (b) in
phosphorylated state.
Inactivation of pyruvate kinase is brought
about by cAMP-dependent protein kinase.
The hormone glucagon inhibits hepatic
glycolysis by this mechanism.
40. Energy yield from glycolysis
During anaerobic:
One molecule of glucose is converted to 2
molecules of lactate, there is a net yield of 2
molecules of ATP.
4 molecules of ATP are synthesized by 2
substrate level phosphorylation.
2 ATP molecules are used in steps 1 & 3,
Hence, net yield is 2 ATP.
41. During Aerobic condition
2 NADH molecules, generated in the
glyceraldehyde 3P-dehydrogenase
reaction & enter ETC.
NADH provides 3 ATP, this reaction
generates 3x2=6 ATP
Total ATP is 6+2=8 ATP.
42. Conversion of pyruvate to lactate
In anaerobic condition, pyruvate is reduced
to lactate by lactate dehydrogenase (LDH).
LDH has 5 iso-enzymes.
The cardiac iso-enzyme of LDH will be
increased in myocardial infarcts.
44. Significance of Lactate Production
The NADH is obtained from the reaction
catalysed by glyceraldehyde 3-phosphate
dehydrogenase.
The formation of lactate allows the
regeneration of NAD+ which can be reused by
glyceraldehyde 3-phosphate dehydrogenase.
Glycolysis proceeds even in the absence of
oxygen to supply ATP.
46. Glycolysis is very essential in skeletal muscle
during strenous exercise where oxygen
supply is very limited.
In RBCs, there are no mitochondria.
Glycolysis in the erythrocytes leads to
lactate production
RBCs derive energy only through glycolysis,
where the end product is lactic acid.
47. Lactic acidosis
Elevation of lactic acid in the circulation
(normal plasma 4-15 mg/dl) may occur due to
its increased production or decreased
utilization.
Mild forms of lactic acidosis are associated
with strenuous exercise, shock, respiratory
diseases, cancers, low PDH activity, von
Gierke's disease etc.
48. Severe forms of lactic acidosis are observed
due to impairment/collapse of circulatory
system -in myocardial infarction, pulmonary
embolism, uncontrolled hemorrhage & severe
shock.
This type of lactic acidosis is due to
inadequate supply of O2 to the tissues with a
drastic reduction in ATP synthesis, which may
lead to death.
49. Oxygen debt refers to the excess amount
of O2 required to recover.
Measurement of plasma lactic acid is
useful to know about the oxygen debt,
and monitor the patient's recovery.
50. Pasteur effect
The inhibition of glycolysis by oxygen
(aerobic condition) is known as Pasteur
effect.
Pasteur effect is due to the inhibition of the
enzyme phosphofructokinase.
Glycolytic intermediates from fructose 1,6-
bisphosphate onwards decrease while the
earlier intermediates accumulate.
51. Crabtree effect
Inhibition of oxygen consumption by the
addition of glucose to tissues having high
aerobic glycolysis is known as Crabtree effect.
Opposite to that of Pasteur effect.
Crabtree effect is due to increased competition
of glycolysis for inorganic phosphate (Pi) &
NAD+ which limits their availability for
phosphorylation & oxidation.