- Genes can be structural or regulatory, with regulatory genes controlling the expression of other genes.
- Homeotic genes control embryonic development and malfunctions can result in body parts appearing in abnormal locations.
- Gene expression involves transcription of DNA into mRNA, which is then translated into proteins. Regulatory elements and splicing modify gene transcripts.
Concept of gene & ultra structure of geneJigar Patel
This presentation includes introduction of gene, gene concept, chemical composition and ultra structure of prokaryotic and eukaryotic gene for B.Sc students.
cell lineage , cell fate - diverse class of cell fate, cell fate in plant meristem, mammalian development cell fate, nutritional effects on epigenetics, epigenetics of plants,
control of cell fate.
Slides about Cell Fate, Cell Potency, Differentiation, Specification, Modes of Specification, Role of Cytoplasm. Cell Interactions, Regulation in Development
Concept of gene & ultra structure of geneJigar Patel
This presentation includes introduction of gene, gene concept, chemical composition and ultra structure of prokaryotic and eukaryotic gene for B.Sc students.
cell lineage , cell fate - diverse class of cell fate, cell fate in plant meristem, mammalian development cell fate, nutritional effects on epigenetics, epigenetics of plants,
control of cell fate.
Slides about Cell Fate, Cell Potency, Differentiation, Specification, Modes of Specification, Role of Cytoplasm. Cell Interactions, Regulation in Development
this presentation covers about all the topics of nucleic acids.I made this presentation by combining too many presentations. and I also presented the same in the university and I got an A++ :).
best of luck!
• Define transcription• Define translation• What are the 3 steps.pdfarihantelehyb
• Define transcription
• Define translation
• What are the 3 steps of translation?
• Define the “genetic dogma”
• What is the function of Transfer RNA?
• What is the function of RNA polymerase?
• What is the function of DNA polymerase?
• Define “splicing of RNA”
• What is an exon?
• What component of the cell does the translation?
• What molecule in the cell does transcription?
• What are the functions of: operon, promotor?
• What is the difference between inducible operon and repressible operon?
Solution
• Define transcription
Transcription is the process of making an RNA copy of a gene sequence. This copy, called a
messenger RNA (mRNA) molecule, leaves the cell nucleus and enters the cytoplasm, where it
directs the synthesis of the protein, which it encodes. Here is a more complete definition of
transcription.
• Define translation
Translation is the process of translating the sequence of a messenger RNA (mRNA) molecule to
a sequence of amino acids during protein synthesis. The genetic code describes the relationship
between the sequence of base pairs in a gene and the corresponding amino acid sequence that it
encodes. In the cell cytoplasm, the ribosome reads the sequence of the mRNA in groups of three
bases to assemble the protein. Here is a more complete definition of translation:
• What are the 3 steps of translation?
Step # 1. Initiation:
Initiation of translation in E .coli involves the small ribosome subunit, a mRNA molecule, a
specific charge initiator tRNA, GTP, Mg++ and number of proteinaceous initiation factors (IFs).
These are initially part of the small subunit and are required to enhance binding affinity of the
various translational components (Table 8.1). Unlike ribosomal proteins, IFs are released from
the ribosome once initiation is completed.
Step # 2. Elongation:
Once both subunits of the ribosome are assembled with the mRNA, binding site for two charged
tRNA molecules are formed. These are designated as the ‘P’ or peptidyl and the ‘A’ or
aminoacyl sites. The charged initiator tRNA binds to the P site, provided that the AUG triplet of
mRNA is in the corresponding position of the small subunit. The increase of the growing
polypeptide chain by one amino acid is called elongation.
Step # 3. Termination:
Termination of protein synthesis is carried out by triplet codes (UAG, UAA, UGA; stop codons)
present at site A. These codons do not specify an amino acid, nor do they call for a tRNA in the
A site. These codons are called stop codons, termination codons or nonsense codons. The
finished polypeptide is still attached to the terminal tRNA at the P site, and the A site is empty.
• Define the “genetic dogma”
A theory in genetics and molecular biology subject to several exceptions that genetic information
is coded in self-replicating DNA and undergoes unidirectional transfer to messenger RNAs in
transcription which act as templates for protein synthesis in translation
• What is the function of Transfer RNA?
The tRNA molecule, or tr.
Control of gene expression ppt
definition of gene expression
inducible gene expression
repressible gene expression
control of gene expression in eukaryotics .all the in information about this topic is include .
Replication,transcription,translation complete the central dogma of life.How mRNA,tRNA,rRNA act on ribosomes for protein synthesis.Difference between eukaryotes and prokaryotes
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
Normal Labour/ Stages of Labour/ Mechanism of LabourWasim Ak
Normal labor is also termed spontaneous labor, defined as the natural physiological process through which the fetus, placenta, and membranes are expelled from the uterus through the birth canal at term (37 to 42 weeks
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
2. Gene Function Structural genes Become part of the structure & functioning of an organism Regulatory genes Control the action of other genes ie. “switch genes on or off” Or control rate of production of proteins Can switch genes on or off in one of two ways
3. Two types of gene regulation Regulatory genes may code for a DNA-binding protein These have a positively charged binding site that will enable it to bind to DNA They will bind to a region near a gene and directly turn it on or off Regulatory genes may code for a signalling protein This will bind to a receptor on the cell membrane Genes will be turned on or off via signal transduction
4. Homeotic Genes “Master genes” that control embryonic development in insects and vertebrates. A malfunctioning homeotic gene in flies may result in wings, legs, antennae and halteres being absent, or appearing in places that they should not.
5. Homeotic Genes In humans, homeotic genes fall in to 4 groups (Hox A,B,C & D). These encompas 39 genes spread over 4 chromosomes Malfunction of HOXC8 results in an extra pair of ribs Malfunction of HOXD13 results in an extra digit between digits 3 & 4 (often fused)
6. Gene Structure The arrangement of base pairs (c) in a piece of double stranded DNA (d) will determine many things, such as the coding and non-coding portions of DNA (a), and the length of a gene (b). Thus many representations of the same strand required.
7. Gene Structure Enzymes need to know when to start and stop reading a section of DNA. If the base pairs were a sentence, regulatory genes may be likened to capital letters and full stops. 5’ 3’ 3’ 5’ Regulatory gene START STOP Promoter region Terminator region
8. Gene Expression Our DNA is like the master plan for building an organism Genes are specific instructions on how to build one tiny part of the entire organism. Genes are located on the DNA, in the nucleus of our cells The mechanisms for making the products for which these genes code are in the cytoplasm How does the message get out of the nucleus and in to the cytoplasm?
9. Gene Expression In order to be expressed, DNA must be transcribed in to mRNA. A C A T A G G C T T G T A T C C G A
10. Transcription After the complimentary strand is unzipped, the RNA is written against the template strand of DNA A C A T A G G C T U G U A U C C G A 1 2
11. Transcription: step-by-step The enzyme RNA polymerase attaches to the DNA in the upstream (3’) region of the template strand on the promoter sequence. The double-stranded DNA helix unwinds As RNA polymerase moves down the strand, complimentary RNA bases are put down in a 5’ to 3’ direction. A methylated cap is added to the 5’ end of the mRNA The transcribed portion of the helix recoils once it has provided a template for the mRNA bases
12. Transcription: continued Once RNA polymerase reaches the terminator sequence a hairpin loop forms in the mRNA, causing it to be released Poly-A polymerase cleaves the end of the mRNA and synthesises a poly-A tail (adenine bases and proteins). A single stranded molecule called pre-messenger RNA (pre-mRNA) is produced
13. Post-transcription modification The DNA in Eukaryotic genes is made up of ... Introns (non-coding sequences) Exons (coding sequences) Prokaryotic DNA does not contain introns The entire gene is copied during transcription, so it is necessary to the spice out the introns Exon Exon Exon Intron Intron
14. Post-transcription modification Introns are removed by a spliceosome, which is made up of a bundle of protein factors called snerps (snRNP) The introns are coiled in to a shape called a lariat and released The remaining exons are then joined together.
15. One gene, multiple products Research reveals that a single gene is able to make a different product at different stages of development Also, a single gene can make one type of product in one type of tissue and a different product in another type of tissue tissue How is this possible? The human genome contains approx. 25,000 genes Yet there are approx. 120,000 recognised protein-coding mRNA sequences. How is this possible?
16. Alternative splicing of pre-mRNA A) Intron retention The final product can look quite different if not all introns are spliced out B) Exon juggling Exons can be recombined in a anumber of different combinations
17. Translation The mature mRNA moves out of the nucleus, through a nuclear pore, in to the cytoplasm Ribosome assembles around mRNA and sequence of bases is read in blocks of 3 bases known as triplets ( = 1 codon) A transfer RNA (tRNA) molecule with the complementary anticodon is brought in and attaches to the mRNA The AUG triplet is the “start” codon
18.
19. Codons with corres-ponding amino acids The genetic code is universal 99.9% of species use the same triplet code for the same amino acid
20.
21. Prokaryotes vs Eukaryotes Where transcription / translation occurs Eukaryotes: nucleus then cyctoplasm Prokaryotes: cytoplasm Speed at which it occurs Slower in Eukaryotes due to necessity to move out to cytoplasm as well as time required to splice mRNA Life span of mRNA Prokaryotes: a few minutes Eukaryotes: hours/days to allow time for p/t modification Ribosomes Eukaryotic ribosomes are larger and have a different rRNA to protein ratio
22.
23. Gene regulation in Prokaryotes CASE STUDY: THE LAC OPERON Bacteria have groups of genes that are controlled together and are turned on/off as required The LAC operon is a group of genes that produce the enzymes required to preak down lactose to glucose and galactose The bacterium only wants to produce these enzymes when lactose is present.
24. The LAC Operon Usually a repressor protein (produced by LAC regulatory gene) sits on the controlling region When lactose enters the cell it binds to the repressor, and the repressor releases from the DNA The LAC genes will now start transcribing mRNA, which will enter a ribosome and produce the 3 enzymes required for lactose metabolism When concentration of lactose in the cell decreases, the lactose is released from the repressor and it returns to inhibiting the operon
25. Not all genes produce proteins Instead of mRNA, genes can also be transcribed as: tRNA: then move out in to the cytoplasm as a transfer molecule rRNA: then move in to the cytoplasm to form part of a ribosome The nucleolus is a region in the nucleus where rRNA is transcribed and stored until required
26. Mitochondrial DNA In Eukaryotes, mitochondrial DNA (mtDNA) is a double-stranded circular molecule In humans, it encompasses only 16,568 base pairs and 37 genes in total. Apart from the genes coding for tRNA and rRNA, the rest are involved in cellular respiration. Mitochondrial DNA is inherited entirely along maternal lines.