Polymerase chain reaction (PCR) is a technique used to amplify DNA sequences. It was developed by Kary Mullis in 1983 and has become a common technique used in medical and biological research. Mullis received the Nobel Prize in Chemistry in 1993 for his work on PCR. PCR involves denaturing DNA, annealing primers to the DNA, and extending the primers to replicate the DNA in multiple cycles, exponentially amplifying any specific DNA region. There are various types of PCR including conventional PCR, multiplex PCR, nested PCR, quantitative PCR, and real-time PCR which have various applications such as disease diagnosis, DNA sequencing, and phylogeny analysis.