Epigenetic mechanisms involve heritable changes in gene expression that do not alter DNA sequences. There are several epigenetic mechanisms including chromatin remodeling, histone modification, DNA methylation, and non-coding RNA pathways. [1] The epigenetic code in each cell, defined by DNA methylation and histone modifications, gives each cell its unique identity while the underlying genetic code remains the same. [2] Histone modifications like acetylation and methylation can activate or repress gene expression by changing chromatin structure and accessibility of DNA to transcription factors. [3]
Dna methylation ppt
definition of Dna methylation ppt
discovery of Dna methylation ppt
types of Dna methylation ppt
history of Dna methylation ppt
process of Dna methylation ppt
mechanism of Dna methylation ppt
methylation in cancer
cytosine methylation
genomic imprinting
This presentation on Epigenetics is most advanced and evidence based one. Its Very helpful for Genetics students and research fellows, Reproductive Medicine specialist, Reproductive Biologist, Infertility practitioners
Epigenetics is the study, in the field of genetics, of cellular and physiological phenotypic trait variations that are caused by external or environmental factors that switch genes on and off and affect how cells read genes instead of being caused by changes in the DNA sequence. -Wikipedia
Dna methylation ppt
definition of Dna methylation ppt
discovery of Dna methylation ppt
types of Dna methylation ppt
history of Dna methylation ppt
process of Dna methylation ppt
mechanism of Dna methylation ppt
methylation in cancer
cytosine methylation
genomic imprinting
This presentation on Epigenetics is most advanced and evidence based one. Its Very helpful for Genetics students and research fellows, Reproductive Medicine specialist, Reproductive Biologist, Infertility practitioners
Epigenetics is the study, in the field of genetics, of cellular and physiological phenotypic trait variations that are caused by external or environmental factors that switch genes on and off and affect how cells read genes instead of being caused by changes in the DNA sequence. -Wikipedia
"Epigenetics refers to genetic factors that change an organism’s appearance or biological functions without changing the actual DNA sequence. In other words, gene expression changes but the genes themselves don’t. Epigenetics adds an additional level of complexity to the genetic code." - Public Health Cafe
Epigenetics definition, history of epigenetics, molecular basis of epigenetics, epigenetic modification, tools to study epigenetics, disease linked with epigenetics, DNA methylation demethylation and enzymes regulating DNA methylation
The epigenetic regulation of DNA-templated processes has been intensely studied over the last 15
years. DNA methylation, histone modification, nucleosome remodeling, and RNA-mediated targeting regulate many biological processes that are fundamental to the genesis of cancer. Here, we
present the basic principles behind these epigenetic pathways and highlight the evidence suggesting that their misregulation can culminate in cancer. This information, along with the promising clinical and preclinical results seen with epigenetic drugs against chromatin regulators, signifies that it
is time to embrace the central role of epigenetics in cancer.
Overview of epigenetics and its role in diseaseGarry D. Lasaga
Epigenetics is the study of heritable changes in gene expression (active versus inactive genes) that do not involve changes to the underlying DNA sequence — a change in phenotype without a change in genotype — which in turn affects how cells read the genes.
Short intro epigenetics & nutrigenomics& the early impact of nutrition Norwich Research Park
Our “genes” are not fixed: “Plasticity” of the genotype by epigenetic mechanisms => important for the phenotypic impact of nutrition.
• Histone and DNA modifications have impact on gene transcription efficiency. Methylation (more stable) and acetylation (more flexible) have impact on chromatin
structures.
• Epigenetic modifications have impact on offspring, embryo development, ageing and disease development or prevention => example: Dutch Hunger Winter.
Health status of future parents are very important for the future health of children.
Early healthy nutrition & lifestyle essential for successful healthy life & “ageing”.
Long-lasting alterations to DNA methylation and ncRNAs could underlie the eff...Ben Laufer
Fetal alcohol spectrum disorders (FASDs) are characterized by life-long changes in gene expression, neurodevelopment and behavior. What mechanisms initiate and maintain these changes are not known, but current research suggests a role for alcohol-induced epigenetic changes. We assessed alterations to adult mouse brain tissue by assaying DNA cytosine methylation and small noncoding RNA (ncRNA) expression, specifically the microRNA (miRNA) and small nucleolar RNA (snoRNA) subtypes. We found long-lasting alterations in DNA methylation as a result of fetal alcohol exposure, specifically in the imprinted regions of the genome harboring ncRNAs and sequences interacting with regulatory proteins. The findings of this study help to expand on the mechanisms behind the long-lasting changes in the brain transcriptome of FASD individuals.
Webinar Link: http://www.youtube.com/watch?v=fzdc0GIdCnA
"Epigenetics refers to genetic factors that change an organism’s appearance or biological functions without changing the actual DNA sequence. In other words, gene expression changes but the genes themselves don’t. Epigenetics adds an additional level of complexity to the genetic code." - Public Health Cafe
Epigenetics definition, history of epigenetics, molecular basis of epigenetics, epigenetic modification, tools to study epigenetics, disease linked with epigenetics, DNA methylation demethylation and enzymes regulating DNA methylation
The epigenetic regulation of DNA-templated processes has been intensely studied over the last 15
years. DNA methylation, histone modification, nucleosome remodeling, and RNA-mediated targeting regulate many biological processes that are fundamental to the genesis of cancer. Here, we
present the basic principles behind these epigenetic pathways and highlight the evidence suggesting that their misregulation can culminate in cancer. This information, along with the promising clinical and preclinical results seen with epigenetic drugs against chromatin regulators, signifies that it
is time to embrace the central role of epigenetics in cancer.
Overview of epigenetics and its role in diseaseGarry D. Lasaga
Epigenetics is the study of heritable changes in gene expression (active versus inactive genes) that do not involve changes to the underlying DNA sequence — a change in phenotype without a change in genotype — which in turn affects how cells read the genes.
Short intro epigenetics & nutrigenomics& the early impact of nutrition Norwich Research Park
Our “genes” are not fixed: “Plasticity” of the genotype by epigenetic mechanisms => important for the phenotypic impact of nutrition.
• Histone and DNA modifications have impact on gene transcription efficiency. Methylation (more stable) and acetylation (more flexible) have impact on chromatin
structures.
• Epigenetic modifications have impact on offspring, embryo development, ageing and disease development or prevention => example: Dutch Hunger Winter.
Health status of future parents are very important for the future health of children.
Early healthy nutrition & lifestyle essential for successful healthy life & “ageing”.
Long-lasting alterations to DNA methylation and ncRNAs could underlie the eff...Ben Laufer
Fetal alcohol spectrum disorders (FASDs) are characterized by life-long changes in gene expression, neurodevelopment and behavior. What mechanisms initiate and maintain these changes are not known, but current research suggests a role for alcohol-induced epigenetic changes. We assessed alterations to adult mouse brain tissue by assaying DNA cytosine methylation and small noncoding RNA (ncRNA) expression, specifically the microRNA (miRNA) and small nucleolar RNA (snoRNA) subtypes. We found long-lasting alterations in DNA methylation as a result of fetal alcohol exposure, specifically in the imprinted regions of the genome harboring ncRNAs and sequences interacting with regulatory proteins. The findings of this study help to expand on the mechanisms behind the long-lasting changes in the brain transcriptome of FASD individuals.
Webinar Link: http://www.youtube.com/watch?v=fzdc0GIdCnA
Prokaryotic cells do not contain nuclei or other membrane-bound organelles.
The nucleoid is the area of a prokaryotic cell in which the chromosomal DNA is located.
Chromosome is several orders of magnitude larger than the cell itself.
So, if bacterial chromosomes are so huge, how can they fit comfortably inside a cell—much less in one small corner of the cell?
Most prokaryotes do not have histones (except some species of Archaea).
Thus, one way prokaryotes compress their DNA into smaller spaces is through supercoiling.
Most bacterial genomes are negatively supercoiled during normal growth.
Multiple proteins act together to fold and condense prokaryotic DNA.
One most abundant protein HU, found in the nucleoid, works with topoisomerase I to bind DNA and introduce sharp bends in the chromosome, Generating the tension necessary for negative supercoiling.
Recent studies… other proteins like integration host factor (IHF), can bind to specific sequences within the genome and introduce additional bends.
The folded DNA is then organized into a variety of conformations that are supercoiled and wound around tetramers of the HU protein, much like eukaryotic chromosomes are wrapped around histones.
Mesenchymal and epithelial differentiation ability of corneal stem cellsPeyman Ghoraishizadeh
: The corneal stroma is being increasingly recognized as a repository for stem cells. Like the limbal and endothelial niches, stromal stem cells often reside in the peripheral cornea and limbus. These peripheral and limbal corneal stromal cells (PLCSCs) are known to produce mesenchymal stem cells in vitro. Recently, a common corneal stromal and epithelial progenitor was hinted at. This study aims to examine the stem cell potential of corneal stromal cells and to investigate their epithelial transdifferentiation ability
Human amniotic fluid cells (hAFCs) may differentiate into multiple cell lineages and thus have a great potential to become a donor cell source for regenerative medicine. The ability of hAFCs to differentiate into germ cell and oocyte-like cells has been previously documented. Herein we report the potential use of hAFCs to help restore follicles in clinical condition involving premature ovarian failure.
Advenced molecular techniques in molecular medical genetics laboratory
Definition of epigenetics
1. Epigenetic Mechanisms
By
Peyman Ghoraishizadeh
Department of Obstetric& Gynecology
Universiti Putra Malaysia
2. Definition of Epigenetics
• Changes in gene expression or phenotype that don’t involve changes
to the DNA sequence (1)
• Its defined as heritable changes in gene activity and expression that
occur without alteration in DNA sequence (2)
3. Mechanism of Epigenetics
• Mechanisms
Chromatin Remodelling & Histone Modification
DNA Methylation
Non-coding RNA mediated pathway
Prion
Epigenetic code:
Consist of Both DNA methylation and histone modifications
Defining code in every eukaryotic cells
Genetic code in each cell is the same
Epigenetic code is tissue and cell specific (3)
4. Chromatin structure
• Chromatin= DNA+ Protein
• Basic unit of chromatin is nucleosome
• Chromatin structure is dynamic
6. Nucleosome
• nucleosome contains
147 bp of genomic
DNA wrapped around
an octamer of
histone Proteins
approximately 1.75
times
7. Chromatin remodeling
• Chromatin remodeling is accomplished through
two main mechanisms:
• Post trans translational modification of the amino acids
that make up histone protein
• Addition of methyl group to DNA
8. Histone Modifications
• Post-translational modifications:
• – Acetylation – Lys
• – Methylation – Lys and Arg
• – Phosphorylation – Ser and Thr
• – Ubiquitination – Lys
9. Histone Acetylation&Deacetylation
• Histone acetylation
• – Histone acetyl transferases (HATs)
• Adds acetyl groups to histone tails
• Reduces positive charge and weakens interaction of histones with DNA
• Facilitates transcription by making DNA more accessible to RNA
polymerase II
• Histone deacetylation
• – Histone deacetylases (HDACs)
• Removes acetyl groups from histone tails
• Increases interaction of DNA and histones
• Represses transcription (usually)
10. Histone Acetylation&Deacetylation
• Acetylation has two functions:
reduce the positive charge on the lysine residues
Destabilise interactions between histone tails
and structural proteins
11. Histone Methylation
• Histone methylation
• Histone methyl transferases (HMTs)
– Histone lysine methyl transferases(HKMTs)
.Methylate lys (k) residues
. Protein argenin methyl transferase (PRMTs)
.Methylate arge(R) residues
Methylation can result in activation or repression
Of expression
trimethylation of histone H3 at lysine 4 (H3K4) is an active mark for transcription
dimethylation of histone H3 at lysine 9 (H3K9), a signal for transcriptional silencing (4)
13. DNA Methylation
• Involves the addition of a methyl group to DNA
• Usually to the number 5 carbon of the cytosine
• pyrimidine ring
• Effect: reducing gene expression
• Catalyzed by DNA methyltransferases (DNMTs)
• Predominantly found found in CpG sites of mammalian genome
Silencing:
• Methylation of CpG sites within the promoters of genes can lead to their
silencing, a feature found in a number of human cancers (eg. silencing of tumor
• suppressor genes)
Activation:
• In contrast, the hypomethylation of CpG
sites has been associated with the over-expression of oncogenes within cancer cells.
15. References
• 1.http://en.wikipedia.org/wiki/Epigenetics
• 2.Bird A.Nature ,447 ,396-98[2007]
• 3. Turner B (2007). "Defining an epigenetic code". Nat Cell Biol 9 (1): 2–6.
• 4.Gupta, Swati; Se Y. Kim, Sonja Artis, David L. Molfese, Armin Schumacher, J. David Sweatt, Richard E. Paylor, and Farah D. Lubin (10 March 2010). "Histone Methylation Regulates
Memory Formation". The Journal of Neuroscience 30 (10): 3589–3599