1. Human umbilical cord mesenchymal
stem cells attenuate cisplatin-induced
acute and chronic renal
injury

2. INTRODUCTION

3. MATERIALS AND METHODS
hucMSCs isolation and identification
CM-Dil cell-labelling
Cell co-culture
Cisplatin-induced renal injury rat model (6.0 mg/kg)
hucMSCs transplantation (intraperitoneally injection)
Renal function and Malondialdehyde (MDA) determination
Histopathology and Masson’s trichrome staining
TUNEL staining and immunohistochemistry
Quantitative RT-PCR
Western blot

4. hucMSCs accelerate the recovery of renal function in cisplatin-induced
AKI rats
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5. hucMSCs promote proliferation of and inhibit
apoptosis and alleviate inflammation in cisplatin-induced
AKI rats in the renal tubular cells of cisplatin-induced
AKI rats

6. hucMSCs protect renal cell mitochondria from
dysfunction and inhibit the activation of mitochondrial
apoptosis signaling

7. Effects of hucMSCs on rat renal function indexes and histology

8. Inhibition of renal interstitial fibrosis by hucMSC injection

9. hucMSCs inhibit epithelial-mesenchymal transition in
renal cells in vivo and in vitro

10. Conclusion
In conclusion,results demonstrate that hucMSCs attenuate cisplatin-induced acute
and chronic renal injury through preventing mitochondria dysfunction, inhibiting
the activation of mitochondrial signaling pathway and suppressing the
development of EMT.
These findings suggest that hucMSC transplantation is a useful approach for the
therapy of cisplatin-induced AKI and the subsequent renal interstitial fibrosis.