Central dogma
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The document outlines the three major stages of transcription and translation: 1) replication, where DNA is copied during cell division; 2) transcription, where part of a DNA strand is copied into mRNA; and 3) translation, where the mRNA is used by the ribosome to produce a polypeptide based on the mRNA codons. During translation, tRNAs bring amino acids to the ribosome which link them together based on the mRNA codons to form a protein.
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TRANSLATION
Messenger RNA carries genetic code from DNA and is translated by the ribosome into proteins. This involves transfer RNA molecules that associate amino acids with their codons. Translation begins with initiation factors recruiting the small ribosomal subunit to the start codon. Elongation then occurs through peptide bond formation catalyzed by the ribosome and translocation of transfer RNAs. Termination occurs when a stop codon is reached. Translation is highly conserved and essential for protein synthesis in all organisms.
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In Eukaryotic transcription, synthesis of RNA occurs in the 3’→5’ direction. The 3’ end is more reactive due to the hydroxide group. 5’ end containing phosphate groups meanwhile, is not very reactive when it comes to adding new nucleotides. In Eukaryotes, the whole genome is not transcribed at once. Only a part of the genome is transcribed which also acts as the first, principle stage of genetic regulation.
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Polyadenylation
Polyadenylation is the addition of a poly(A) tail to messenger RNA. The poly(A) tail consists of multiple adenosine monophosphates and plays an important role in mRNA stability, nucleocytoplasmic export, and translation. Cleavage and polyadenylation is controlled by cis elements located upstream and downstream of the polyadenylation site and involves endonuclease cleavage of the pre-mRNA followed by poly(A) polymerase addition of adenines in a template-independent manner. Alternative polyadenylation and cytoplasmic polyadenylation allow for regulation of mRNA expression through differing poly(A) tail lengths.
RNA SPLICING
RNA splicing is a process where introns are removed from precursor messenger RNA (pre-mRNA) and exons are joined together to produce mature mRNA. It occurs in the nucleus and is essential for eukaryotes to produce proteins. The spliceosome, a large complex of RNA and proteins, facilitates two transesterification reactions that remove introns and ligate exons. RNA splicing generates protein diversity through alternative splicing and is important for cellular functions and disease processes.
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Non-coding RNAs (ncRNAs) are functional RNA molecules that are not translated into proteins. There are several types of ncRNAs that play important roles in biological processes. tRNAs help translate nucleotides into amino acids during protein synthesis. rRNA and snoRNAs are involved in ribosome and RNA structure/modification. MiRNAs regulate gene expression by binding to mRNA. LncRNAs regulate processes like chromatin structure and transcription. Mt-tRNAs specific to mitochondria are essential for oxidative phosphorylation. Mutations can cause diseases like myopathies.
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Mitochondria contain their own circular genome that is 16.5kb in size and located in the mitochondrial matrix. The mitochondrial genome contains 37 genes that encode 13 proteins, 22 tRNAs, and 2 rRNAs. These genes help produce enzymes and proteins that are crucial for oxidative phosphorylation and energy production in mitochondria. The control region of mitochondrial DNA contains signals that regulate mitochondrial DNA and RNA synthesis.
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This document summarizes key processes in DNA replication, transcription, translation, and their regulation in prokaryotes and eukaryotes. It describes how DNA makes copies of itself semiconservatively. Transcription involves RNA polymerase making an RNA copy of a DNA template. Translation uses ribosomes to convert the RNA into a polypeptide chain. Each process has initiation, elongation, and termination steps. Regulation ensures processes only occur at the right times and locations in the cell.
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DNA structure replication transcription translation
DNA is made up of four nucleotides that form a double helix structure. Watson and Crick discovered that DNA replicates in a semi-conservative manner, with each new DNA molecule composed of one original strand and one newly synthesized strand. DNA replication is highly regulated and involves several enzymes that unwind, proofread, and repair the DNA to replicate it with high fidelity. The information stored in DNA is used to direct the synthesis of proteins according to the central dogma of biology, with DNA being transcribed into RNA which is then translated into proteins.
Dna replication and enzymes involved in dna replication
DNA replication is the process by which DNA copies itself. It requires DNA polymerase enzymes, primers, and deoxynucleotide triphosphates. DNA polymerase adds nucleotides to the 3' end of the primer according to the DNA template. Replication occurs through the coordinated action of helicase, DNA polymerase, primase, ligase, and other proteins. DNA polymerase proofreads newly synthesized DNA to ensure high fidelity. The coordinated actions of these enzymes and proteins precisely duplicate the genome during semi-conservative replication.
DNA Presentation
This document covers DNA replication, transcription, translation and gene expression. It discusses how DNA is copied through semi-conservative replication involving enzymes like DNA polymerase and how genes are expressed through transcription of DNA to mRNA and translation of mRNA to proteins. It also summarizes control of gene expression in prokaryotes through operons and in eukaryotes at multiple stages. Finally, it briefly touches on gene mutations, cancer and genetic modification of plants.
Chapter 10
1. DNA contains genetic instructions that can be copied and passed from generation to generation. It is made up of nucleotides with four bases that form base pairs to create a double helix structure.
2. DNA replication is the process where the DNA double helix unwinds and each strand serves as a template to make a new complementary strand, duplicating the DNA within the cell.
3. Genetic information flows from DNA to RNA to protein. Through transcription, DNA is used as a template to make messenger RNA (mRNA), which carries the genetic code to be translated by ribosomes into proteins.
Basic Biological Concepts & Basic Genetics
The document discusses basic biological concepts and genetics. It defines cells as the basic unit of life and describes prokaryotic and eukaryotic cell structures. It also summarizes Mendel's experiments with pea plants which demonstrated that traits are passed from parents to offspring through discrete units now called genes, and that genes assort independently during reproduction.
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Replication, transcription, translation and its regulation
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Similar to Central dogma
Dna replication copy
DNA replication is the process by which a cell makes an identical copy of its DNA before cell division. It involves unwinding the DNA double helix and using each strand as a template to create a new partner strand through complementary base pairing. DNA polymerase adds nucleotides to the new strands to replicate the DNA. This ensures each daughter cell has an exact copy of the original DNA.
Central dogma
DNA is transcribed into mRNA which is then translated into proteins. Transcription involves RNA polymerase making an RNA complement of a DNA sequence, replacing thymine with uracil. Translation involves tRNAs bringing amino acids to ribosomes where they are linked together into polypeptides based on the mRNA code. The ribosome facilitates decoding the mRNA into a protein product.
Dna replication
DNA replication is the process by which a cell makes an identical copy of its DNA before cell division. It involves unwinding the DNA double helix and using each strand as a template to create a new partner strand. DNA polymerase adds complementary nucleotides to each new strand. When complete, the process generates two identical DNA double helices from the original. Before a cell divides, it must replicate its DNA so that the resulting daughter cells have the same genetic information as the parent cell.
lecture 3 Gene expression pptx
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product. It involves three main steps: replication, transcription, and translation. Replication copies DNA, transcription creates mRNA from DNA, and translation uses mRNA to produce proteins. The central dogma states that genetic information flows from DNA to RNA to protein. Mutations can occur and change the nucleotide sequence, potentially altering the amino acid sequence of the resulting protein.
Rna protein-synthesis
This document discusses RNA, ribosomes, and protein synthesis. It explains that DNA is transcribed into messenger RNA, which directs the process of translation where transfer RNA and ribosomes work together to assemble amino acids into proteins according to the genetic code stored in DNA. The central dogma of molecular biology is that DNA makes RNA makes protein, with three types of RNA - messenger RNA, transfer RNA, and ribosomal RNA - all playing important roles in the transfer of genetic information from DNA to protein.
please explain transcription and translationSolutionAnsTran.pdf
please explain transcription and translation
Solution
Ans:
Transcription is the process of making an RNA copy of a gene sequence. This copy, called a
messenger RNA (mRNA) molecule, leaves the cell nucleus and enters the cytoplasm, where it
directs the synthesis of the protein, which it encodes. Translation is the process of translating the
sequence of a messenger RNA (mRNA) molecule to a sequence of amino acids during protein
synthesis. The genetic code describes the relationship between the sequence of base pairs in a
gene and the corresponding amino acid sequence that it encodes. In the cell cytoplasm, the
ribosome reads the sequence of the mRNA in groups of three bases to assemble the protein.
Transcription is the process by which DNA is copied (transcribed) to mRNA, which carries the
information needed for protein synthesis. Transcription takes place in two broad steps. First, pre-
messenger RNA is formed, with the involvement of RNA polymerase enzymes. The process
relies on Watson-Crick base pairing, and the resultant single strand of RNA is the reverse-
complement of the original DNA sequence. The pre-messenger RNA is then \"edited\" to
produce the desired mRNA molecule in a process called RNA splicing.
Formation of pre-messenger RNA
The mechanism of transcription has parallels in that of DNA replication. As with DNA
replication, partial unwinding of the double helix must occur before transcription can take place,
and it is the RNA polymerase enzymes that catalyze this process.
Unlike DNA replication, in which both strands are copied, only one strand is transcribed. The
strand that contains the gene is called the sense strand, while the complementary strand is the
antisense strand. The mRNA produced in transcription is a copy of the sense strand, but it is the
antisense strand that is transcribed.
Ribonucleotide triphosphates (NTPs) align along the antisense DNA strand, with Watson-Crick
base pairing (A pairs with U). RNA polymerase joins the ribonucleotides together to form a pre-
messenger RNA molecule that is complementary to a region of the antisense DNA strand.
Transcription ends when the RNA polymerase enzyme reaches a triplet of bases that is read as a
\"stop\" signal. The DNA molecule re-winds to re-form the double helix.
RNA splicing
The pre-messenger RNA thus formed contains introns which are not required for protein
synthesis. The pre-messenger RNA is chopped up to remove the introns and create messenger
RNA (mRNA) in a process called RNA splicing
Alternative splicing
In alternative splicing, individual exons are either spliced or included, giving rise to several
different possible mRNA products. Each mRNA product codes for a different protein isoform;
these protein isoforms differ in their peptide sequence and therefore their biological activity. It is
estimated that up to 60% of human gene products undergo alternative splicing.
Alternative splicing contributes to protein diversity a single gene transcript (RNA) can have
tho.
Genetics
Genetics is the study of heredity and genetic variation. Key terms include:
- Genotype is the genetic makeup of an organism, phenotype is observable traits.
- Genes hold information to build cells and pass traits to offspring. The human genome contains 25,000-35,000 genes located on 23 chromosome pairs in the nucleus.
- DNA is transcribed to RNA and translated to proteins, which determine an organism's traits. Variations in genes and chromosomes can result in genetic disorders. Common methods to study genetics include karyotyping, analyzing pedigrees, and identifying alleles and mutations. Understanding genetics provides insight into inheritance patterns and human health.
Molecular basis of inheritance-Protein synthesis part 1 Transcriptionein ppt...
The document summarizes the process of protein synthesis, which involves two main steps - transcription and translation. Transcription occurs in the nucleus, where DNA is transcribed into mRNA by RNA polymerase. The mRNA then acts as a messenger to carry the genetic code to the cytoplasm, where translation occurs on ribosomes to synthesize proteins based on the mRNA sequence. The central dogma of molecular biology describes the flow of genetic information from DNA to RNA to protein. Some viruses use reverse transcription to produce DNA from their RNA genetic material.
Ig
The document summarizes DNA, RNA, the genetic code, and protein biosynthesis. It discusses the central dogma of molecular biology whereby DNA is transcribed into mRNA which is then translated into proteins. Key steps include DNA replication, transcription, translation, and post-translational processing. The genetic code is explained where codons in mRNA specify amino acids. Recombinant DNA technology allows joining DNA from different sources to produce recombinant proteins.
Chapter 8 Microbial GeneticsPlasm
This document summarizes key concepts in microbial genetics including:
1) Plasmids exist separately from bacterial chromosomes and can transfer genes horizontally.
2) The central dogma of molecular biology describes how DNA is transcribed into RNA and translated into protein.
3) Bacterial gene expression is regulated through operons such as the lac and tryptophan operons which are induced or repressed in response to environmental conditions.
4) Mutations can occur spontaneously or be caused by mutagens and can alter bacterial genes and phenotypes.
Pptgenlec
The central dogma of molecular biology describes the flow of genetic information within cells. It states that information flows from DNA to RNA to protein, but not in the reverse direction. The document then provides more details about DNA replication, transcription, and translation. It explains the basic structures and processes involved, including the key enzymes and molecules. Examples are given of prokaryotic and eukaryotic differences. The lac operon is used as an example of transcriptional control in response to environmental signals.
Brief Concepts and Questions EXAM 2 Chapter 8 DNA RNA Protein What i.pdf
Brief Concepts and Questions EXAM 2 Chapter 8: DNA RNA Protein What is DNA? a
phosphate Structure of DNA: Building blocks are called nucleotides Each nucleotide is
composed of three br uithofenas bee. What makes DNA so special? Provide 4 reasons, below
DNA DNA (Replication): Where does DNA replication take place? When does DNA replication
take place? Explain steps involved in DNA replication: DNA RNA Protein (Gene Expression)
Involves 2 processes: 1. Transcription 2. Translation Explain the Synthesis of Proteins (Gene
Expression): o DNA RNA Protein What is RNA? What is \"codon What is \"anticodon\" What is
a protein molecule? DNA mutation; Change in nucleotide bases of DNA Duplex Point mutation
Frame shift mutation
Solution
Question
Answer
Where does DNA replication take place:
It takes place in the nucleus in case of eukaryotic cells and in the cytoplasm in case of
prokaryotic cells
When does DNA replication take place:
DNA replication occurs during the S-phase during cell cycle, so that cell can make an extra copy
of genetic material.
Explain steps involved in DNA replication:
Initiation: During initiation, the proteins will bind to the origin of replication; helicase unwinds
the DNA helix which results in the formation of two replication forks.
Elongation: A RNA primer sequence will be added to this the DNA pol III will add the
nucleotides in 5’ to 3’ direction and chain will elongate.
Termination: In case of bacteria, termination of replication occurs whenever two replication
forks meet each other from the opposite end of the parental chromosome.
Transcription
Gene expression first step is transcription, here a particular segment of DNA will be copied into
RNA with the help of the enzyme RNA polymerase
Translation
Translation is the final step of the gene expression. Here mRNA will be used to synthesize the
polypeptide chain. The information present in the mRNA in the form of codon will code for the
amino acids needed for polypeptide chain synthesis.
What is RNA?
RNA is ribonucleic acid and is found in all living cells. It acts as the messenger carrying
instructions from DNA for the synthesis of proteins.
Few viruses will have RNA as their genetic material.
What is codon?
Codon is a sequence of three nucleotides and they together form a unit of genetic code in either
DNA or RNA.
What is anticodon?
It is found on tRNA and it is a sequence of three nucleotides which forms a genetic code on
tRNA, and these anticodon is complementary to the codons found on messenger RNA.
What is a protein molecule?
During translation, when amino acids are added in a sequential manner, the condensation of
amino acids will form a peptide bond in between them and finally forms a polypeptide chain. It
is the DNA through mRNA directs the protein synthesis.
Point mutation
In point mutation, only one or very few nucleotides will be affected or mutated in a gene
sequence.
Frame shift mutation
Either insertions or deletion can result in frame shift mutation, due to th.
Lesson 2 DNA and RNA.pptx
DNA contains the genetic instructions for living organisms. It is a double-stranded molecule found in the nucleus of cells. DNA is made up of nucleotides, which contain a phosphate group, sugar (deoxyribose), and one of four nitrogen bases (adenine, guanine, cytosine, thymine). The bases on each strand pair up through hydrogen bonding - adenine pairs with thymine and cytosine pairs with guanine. DNA replicates through a semi-conservative process where each new molecule contains one original and one new strand. RNA carries instructions from DNA for protein production and differs from DNA by being single-stranded and containing ribose and uracil instead of thymine.
protein synthesis
1. Transcription is the process by which DNA is copied into messenger RNA (mRNA) by RNA polymerase. This involves three phases - initiation, elongation, and termination.
2. Eukaryotic transcription is more complex than prokaryotic transcription due to multiple RNA polymerases, nucleosomes, separation of transcription and translation, and intron-exon structure of genes.
3. Following transcription, eukaryotic mRNA undergoes processing including capping, polyadenylation, and splicing before being translated into protein by ribosomes.
Transcription,translation and genetic code(cell biology)by welfredo yu
PREPARED BY
Welfredo Lubrico Yu,Jr.
BSEd-BIOLOGICAL SCIENCE 2
COLLEGE OF TEACHER EDUCATION(C.T.E.)
SDSSU-MAIN CAMPUS,TANDAG CITY
AP Bio Ch 10 Power Point
The document summarizes key concepts about gene expression and regulation:
1. DNA contains genes that encode instructions for proteins; during transcription, genes are copied into mRNA which is then translated by ribosomes into proteins.
2. In eukaryotes, mRNA must carry DNA information from the nucleus to the cytoplasm for protein synthesis, since DNA is in the nucleus but protein synthesis occurs in the cytoplasm.
3. Transcription involves copying a gene into mRNA, which then directs ribosomes during translation to synthesize the encoded protein according to the genetic code where RNA codons specify amino acids.
Structure and function of DNA.ppt
The document summarizes key aspects of DNA structure and function. It describes the central dogma of molecular biology whereby DNA is transcribed into RNA and then translated into protein. It explains gene expression and the genetic code using codons. It discusses the structures of nucleic acids, nucleotides, and their polymerization to form DNA and RNA. It also summarizes DNA replication, the different forms of DNA/RNA, and their complementary base pairing.
Notes ch13 website
From DNA to Protein
1. DNA contains genes that provide instructions for building proteins through transcription and translation. RNA is produced through transcription and carries the genetic code from DNA. There are three main types of RNA: mRNA, rRNA, and tRNA.
2. During translation, mRNA attaches to ribosomes where tRNA brings amino acids to add to the growing polypeptide chain according to the mRNA codons until a stop codon is reached. This process synthesizes proteins using the genetic code stored in DNA.
3. Mutations in DNA can occur through changes in single nucleotides or the insertion/deletion of nucleotides. This can alter the mRNA and resulting protein sequence produced.
Similar to Central dogma (20)
please explain transcription and translationSolutionAnsTran.pdf
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Molecular basis of inheritance-Protein synthesis part 1 Transcriptionein ppt...
Molecular basis of inheritance-Protein synthesis part 1 Transcriptionein ppt...
Brief Concepts and Questions EXAM 2 Chapter 8 DNA RNA Protein What i.pdf
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Transcription,translation and genetic code(cell biology)by welfredo yu
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Monitoring and Managing Anomaly Detection on OpenShift
Overview
Dive into the world of anomaly detection on edge devices with our comprehensive hands-on tutorial. This SlideShare presentation will guide you through the entire process, from data collection and model training to edge deployment and real-time monitoring. Perfect for those looking to implement robust anomaly detection systems on resource-constrained IoT/edge devices.
Key Topics Covered
1. Introduction to Anomaly Detection
- Understand the fundamentals of anomaly detection and its importance in identifying unusual behavior or failures in systems.
2. Understanding Edge (IoT)
- Learn about edge computing and IoT, and how they enable real-time data processing and decision-making at the source.
3. What is ArgoCD?
- Discover ArgoCD, a declarative, GitOps continuous delivery tool for Kubernetes, and its role in deploying applications on edge devices.
4. Deployment Using ArgoCD for Edge Devices
- Step-by-step guide on deploying anomaly detection models on edge devices using ArgoCD.
5. Introduction to Apache Kafka and S3
- Explore Apache Kafka for real-time data streaming and Amazon S3 for scalable storage solutions.
6. Viewing Kafka Messages in the Data Lake
- Learn how to view and analyze Kafka messages stored in a data lake for better insights.
7. What is Prometheus?
- Get to know Prometheus, an open-source monitoring and alerting toolkit, and its application in monitoring edge devices.
8. Monitoring Application Metrics with Prometheus
- Detailed instructions on setting up Prometheus to monitor the performance and health of your anomaly detection system.
9. What is Camel K?
- Introduction to Camel K, a lightweight integration framework built on Apache Camel, designed for Kubernetes.
10. Configuring Camel K Integrations for Data Pipelines
- Learn how to configure Camel K for seamless data pipeline integrations in your anomaly detection workflow.
11. What is a Jupyter Notebook?
- Overview of Jupyter Notebooks, an open-source web application for creating and sharing documents with live code, equations, visualizations, and narrative text.
12. Jupyter Notebooks with Code Examples
- Hands-on examples and code snippets in Jupyter Notebooks to help you implement and test anomaly detection models.
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Central dogma
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