This document discusses biotechnology and genetic engineering. It defines biotechnology as utilizing biological entities for human welfare. Some key applications of biotechnology mentioned include using microbes to treat waste, producing antibiotics and insulin through recombinant DNA technology, and developing genetically modified crops and animals. The central concepts of genetic engineering like plasmids, restriction enzymes, DNA ligase, and transforming host cells are explained. Producing human insulin through recombinant DNA technology in E. coli is given as an example of applying these concepts. Careers in biotechnology and top institutes offering related courses in India are also briefly outlined.
introduction to Nanobiotechnology
what is nanotechnology
bionanotechnology
classical biotechnology industrial production using biological system
modern biotechnology from industrial processes to noval therapeutics
modern biotechnology immunological enzymatic and neucleic acid based technology
Dna based technology
self assembly and supramolecular chemistry
formation of ordered structure at nano scale
Application of industrial BiotechnologyGhassan Hadi
The document discusses industrial biotechnology and microbial technology. Microbial technology uses microbes to produce products and services of economic value. It involves isolating microbes, screening them for product formation, improving yields, culturing and harvesting products. Microbes are used to produce metabolites, treat waste, control pests and pathogens, and ferment food. They can enhance nutrient availability as biofertilizers. Microbes also recover metals from ores and desulfurize coal. New technologies allow ethanol to be produced from crop residues rather than just grains. Industrial biotechnology and microbial technology have benefits like low substrate input, high output, environmental friendliness, renewability, and increased efficiency.
Richard Feynman is credited with the birth of nanotechnology in 1959 when he challenged scientists that manipulating matter at the nanoscale was possible if the laws of physics allowed. Nanobiotechnology was initiated in 1980 with the development of atomic force microscopy that enables atomic-level imaging. Nanobiotechnology involves creating functional materials and devices through understanding and controlling matter at the nanometer scale of 1 to 100 nm, where new properties emerge. Applications include biomedical imaging, advanced drug delivery, biosensing, and regenerative medicine.
Transgenic plants and plant biotechnologyAmith Reddy
This document discusses transgenic plants and plant biotechnology. It begins with definitions of key terms like transgene, transgenesis, and transgenic plants. It then provides a brief history of plant breeding, including selective breeding, Mendel's genetics studies, and the disadvantages of traditional breeding. Next, it covers mutation breeding using mutagens or radiation. It discusses the process of transgenic plant creation by inserting foreign genes from sources like animals or bacteria. The remainder of the document details various gene transfer methods in plants, including Agrobacterium-mediated transformation using Ti plasmids, direct transformation techniques like particle bombardment, and methods for detecting inserted genes.
An introduction to biotechnology 27.01.2015 tutorial group g1 (sec a&b)Smita Shukla
This document discusses biotechnology, including its history, applications, and future. Biotechnology uses living organisms to benefit humanity and involves fields like microbiology, agriculture, medicine, forensics, and more. It has a long history including fermentation and selective breeding. Modern biotech also includes genetic engineering. The human genome project provided insights into gene functions and diseases. Biotechnology requires interdisciplinary skills and generates jobs in research, manufacturing, and marketing. It faces challenges in applying genomic insights through gene therapy and personalized medicine.
The document discusses molecular farming, which involves using plants or other organisms to produce valuable proteins or pharmaceuticals. It provides a brief history of molecular farming beginning in 1986. It then discusses various host systems used, including bacteria, yeast, algae, plant cell cultures, transgenic plants, and whole plants or animals. The costs of production are much lower for plant systems compared to other methods. Key plant expression systems include transgenic plants, plant cell suspensions, transplastomic plants, transient expression systems, and hydroponic cultures. Many therapeutic proteins, industrial enzymes, antibodies, and vaccines have been produced in different plant host systems. Some early commercial products included avidin, beta-glucuronidase, and trypsin. Leading
This document provides an overview of recombinant DNA technology. It discusses the basic principles, which involve generating DNA fragments, inserting a selected fragment into a cloning vector, introducing the vector into host cells, and multiplying the recombinant molecules. Key steps include using restriction enzymes to cut DNA at specific sites, ligases to join fragments, and various vectors like plasmids and bacteria to clone the DNA. The document also outlines several applications of rDNA technology, such as producing proteins, diagnosing diseases, and developing genetically engineered plants.
introduction to Nanobiotechnology
what is nanotechnology
bionanotechnology
classical biotechnology industrial production using biological system
modern biotechnology from industrial processes to noval therapeutics
modern biotechnology immunological enzymatic and neucleic acid based technology
Dna based technology
self assembly and supramolecular chemistry
formation of ordered structure at nano scale
Application of industrial BiotechnologyGhassan Hadi
The document discusses industrial biotechnology and microbial technology. Microbial technology uses microbes to produce products and services of economic value. It involves isolating microbes, screening them for product formation, improving yields, culturing and harvesting products. Microbes are used to produce metabolites, treat waste, control pests and pathogens, and ferment food. They can enhance nutrient availability as biofertilizers. Microbes also recover metals from ores and desulfurize coal. New technologies allow ethanol to be produced from crop residues rather than just grains. Industrial biotechnology and microbial technology have benefits like low substrate input, high output, environmental friendliness, renewability, and increased efficiency.
Richard Feynman is credited with the birth of nanotechnology in 1959 when he challenged scientists that manipulating matter at the nanoscale was possible if the laws of physics allowed. Nanobiotechnology was initiated in 1980 with the development of atomic force microscopy that enables atomic-level imaging. Nanobiotechnology involves creating functional materials and devices through understanding and controlling matter at the nanometer scale of 1 to 100 nm, where new properties emerge. Applications include biomedical imaging, advanced drug delivery, biosensing, and regenerative medicine.
Transgenic plants and plant biotechnologyAmith Reddy
This document discusses transgenic plants and plant biotechnology. It begins with definitions of key terms like transgene, transgenesis, and transgenic plants. It then provides a brief history of plant breeding, including selective breeding, Mendel's genetics studies, and the disadvantages of traditional breeding. Next, it covers mutation breeding using mutagens or radiation. It discusses the process of transgenic plant creation by inserting foreign genes from sources like animals or bacteria. The remainder of the document details various gene transfer methods in plants, including Agrobacterium-mediated transformation using Ti plasmids, direct transformation techniques like particle bombardment, and methods for detecting inserted genes.
An introduction to biotechnology 27.01.2015 tutorial group g1 (sec a&b)Smita Shukla
This document discusses biotechnology, including its history, applications, and future. Biotechnology uses living organisms to benefit humanity and involves fields like microbiology, agriculture, medicine, forensics, and more. It has a long history including fermentation and selective breeding. Modern biotech also includes genetic engineering. The human genome project provided insights into gene functions and diseases. Biotechnology requires interdisciplinary skills and generates jobs in research, manufacturing, and marketing. It faces challenges in applying genomic insights through gene therapy and personalized medicine.
The document discusses molecular farming, which involves using plants or other organisms to produce valuable proteins or pharmaceuticals. It provides a brief history of molecular farming beginning in 1986. It then discusses various host systems used, including bacteria, yeast, algae, plant cell cultures, transgenic plants, and whole plants or animals. The costs of production are much lower for plant systems compared to other methods. Key plant expression systems include transgenic plants, plant cell suspensions, transplastomic plants, transient expression systems, and hydroponic cultures. Many therapeutic proteins, industrial enzymes, antibodies, and vaccines have been produced in different plant host systems. Some early commercial products included avidin, beta-glucuronidase, and trypsin. Leading
This document provides an overview of recombinant DNA technology. It discusses the basic principles, which involve generating DNA fragments, inserting a selected fragment into a cloning vector, introducing the vector into host cells, and multiplying the recombinant molecules. Key steps include using restriction enzymes to cut DNA at specific sites, ligases to join fragments, and various vectors like plasmids and bacteria to clone the DNA. The document also outlines several applications of rDNA technology, such as producing proteins, diagnosing diseases, and developing genetically engineered plants.
Genetic engineering involves manipulating genetic material (DNA) to achieve desired goals. The basic principles involve artificially copying DNA from one organism and joining it into the DNA of another. Molecular tools like restriction enzymes and DNA ligases are used to cut and join DNA. Methods to transfer genes include transformation, electroporation, and liposome-mediated transfer. Applications include producing human proteins like insulin, developing gene therapies, and genetically modifying plants. Gene libraries, blotting techniques like Southern blotting, and PCR are also discussed as important molecular tools in genetic engineering.
Biotechnology has been used for millennia to improve agriculture, food production, and medicine through techniques like animal husbandry and fermentation. Modern biotechnology applies scientific principles to processing materials through biological agents. It has applications in medicine like drug development, agriculture like developing pest-resistant crops, and industry like producing chemicals. Biotechnology's scope continues expanding in fields such as genetic engineering, stem cell research, and environmental remediation.
1) The document discusses biosafety and bioethics issues related to microbial technology and biotechnology. It addresses concerns about genetically modified organisms (GMOs) and their impact on human health and the environment.
2) Good manufacturing practices (GMP) are guidelines that ensure products are consistently high quality and safe. They cover all aspects of production to minimize risks.
3) Proper rules and regulations around biosafety are important and vary depending on the organism and its intended use. Biosafety and gaining public trust are crucial to the development and application of biotechnology.
This document discusses several applications of biotechnology in medicine, including the production of human insulin, human growth hormone, vaccines, and gene therapy. It provides details on how recombinant DNA technology is used to produce these therapeutic products. Human insulin is extracted from pancreas cells and inserted into bacterial plasmids to be mass produced. Vaccines like hepatitis B vaccine involve isolating antigen genes and expressing them in yeast or bacteria. Gene therapy approaches like ex vivo therapy aim to correct genetic disorders by isolating cells, adding functional genes, and reinserting the cells.
This document discusses biotransformation, which is a chemical reaction catalyzed by living cells or enzymes. It can be used to modify the functional groups of organic compounds. The document outlines some prerequisites for successful biotransformation processes, including the culture having the necessary enzymes and the substrate not being toxic. It also discusses using plant cell and organ cultures, immobilized cell cultures, and genetic engineering approaches for biotransformation. Some factors that influence biotransformation are also summarized.
Gene cloning involves copying a gene and inserting it into a self-replicating vector to produce multiple copies of the gene. PCR (polymerase chain reaction) is a technique used to amplify specific genes. Both gene cloning and PCR are important for obtaining pure samples of genes and amplifying them for various applications like sequencing, expression of proteins, and genetic engineering.
The document discusses various applications of biotechnology including therapeutics, diagnostics, genetically modified crops for agriculture, and more. It also discusses three critical areas of biotechnology research: providing improved organisms as catalysts, creating optimal conditions for catalysts, and downstream processing technologies. The document then discusses options for increasing food production and how genetically modified crops are seen as a solution. It provides details on genetically modified organisms and Bt cotton as an example, describing how Bt genes provide insect resistance. The document also discusses using RNA interference to protect plants from parasites and the use of recombinant DNA technology in healthcare applications like producing insulin through E. coli. It covers gene therapy, PCR, ELISA, transgenic animals and their uses as well as
Genetic engineering is the process of manipulating genes to introduce desirable traits. It can be used to produce insulin and vaccines, treat genetic disorders through gene therapy or somatic cell gene therapy, and engineer plants and animals. Some applications include producing human growth hormone to treat dwarfism, making human albumin and anti-hemophilic factors, and developing GM crops with traits like pest resistance. However, critics argue that genetic engineering poses environmental and ethical risks by interfering with nature and potentially having irreversible effects.
Transgenic plants are plants that have been genetically modified using genetic engineering techniques to introduce new traits. The goal is to insert desirable genes from other organisms to produce crops with improved traits like pest or disease resistance, increased yield, or tolerance to environmental stresses. Some examples of transgenic crops include insect-resistant corn and cotton, herbicide-resistant soybeans, and golden rice which is enriched with vitamin A. While transgenic crops offer advantages to farmers and consumers, some concerns exist around their impact on human health, the environment, and traditional farming practices. Ongoing research continues to assess both the promises and risks of this emerging agricultural technology.
The document provides an overview of biotechnology, including definitions, key concepts, areas of application, and ethical considerations. It defines biotechnology as using living organisms to produce new products or modify existing organisms. Some main points covered include that biotechnology involves genetics, engineering, agriculture, and manipulating DNA. It also discusses early pioneers in the field and how techniques have advanced from classical to modern biotechnology.
Transformation is the process of altering an organism's genetic makeup by inserting new genes. Common transformation methods include Agrobacterium-mediated transformation, particle bombardment, protoplast transformation using polyethylene glycol or electroporation, and fibre-mediated DNA delivery. Agrobacterium transformation involves the bacteria transferring T-DNA from its Ti plasmid into the plant genome, while direct methods introduce naked DNA into plant cells using physical methods like particle bombardment or chemical treatments that make cell membranes permeable. Transformation allows improving crop traits like yield and stress resistance.
This is part of the MaRS BioEntrepreneurship series.
Speaker: Lynne Zydowsky, Ph.D., Managing Principal Zydowsky Consultants
* Explore the development of regulated drugs and devices
* Understand where and how value is generated in the pharmaceuticals industry
* Appreciate the interplay between science and business in a biotech company
To download a copy of the audio for this presentation, please go to:
http://www.marsdd.com/bioent/oct16
For the event blog and Q+A, please see:
http://blog.marsdd.com/2006/10/17/bringing-together-art-and-science/
The document discusses biobusiness and biosafety, providing definitions and opportunities for biotechnology in developing countries. It examines the market for biobusiness, key opportunity areas, and factors for successful bioenterprise innovation including focusing on high-value opportunities, recognizing that innovation need not have long life cycles, and emphasizing people over technologies. The document also outlines biosafety levels and concepts from containment to facility design to protect laboratory workers and the environment.
Single cell protein (SCP) refers to protein extracted from pure cultures of microorganisms like yeast, algae, fungi and bacteria. It can be used as a protein supplement for humans and animals. SCP is produced by growing microorganisms on substrates through fermentation. The microbes are then harvested, processed and treated to isolate and purify the protein. SCP has potential advantages as a sustainable protein source but also risks if toxic microbes or byproducts are consumed.
The document defines biotechnology as using living organisms to create useful products for humans. It discusses several types of biotechnology including plant biotechnology, which can be used for fruit development, vaccine production, and increasing nutritional quality. Animal biotechnology uses molecular biology techniques to genetically engineer animals for pharmaceutical, agricultural, or industrial applications. Food biotechnology produces the first genetically modified tomato that could be transported without bruising in the early 1990s. Industrial biotechnology uses cells and enzymes to generate industrial products and processes, while medical biotechnology researches and produces pharmaceuticals to treat and prevent diseases using cells and cell material.
This document discusses biotechnology, including its definition, history, applications in different fields like agriculture, medicine, and industry. It covers topics such as drug production using biotechnology techniques, pharmacogenomics, gene therapy, and genetic testing. Drug production through isolation and genetic engineering of enzymes is described. The use of biotechnology to develop medicines and pharmaceuticals for treating diseases is also summarized.
1. The seminar discusses developing transgenic plants resistant to insects through the transfer of resistance genes from microorganisms, higher plants, and animals into crop plants.
2. Major objectives of plant biotechnology are to develop plants resistant to biotic and abiotic stresses. Resistance to insects has been achieved by introducing genes encoding Bt toxins from Bacillus thuringiensis and other insecticidal proteins.
3. Useful genes have been isolated from microbes like B. thuringiensis, higher plants like beans and tobacco, and animals like mammals. These genes have been successfully used to engineer insect-resistant crops like cotton, potato, tomato, and tobacco.
Gene manipulation involves externally arranging genes within an organism and is also known as genetic engineering. It was first used in agriculture to improve plant quality and was discovered in native Mexican corn in 2001. The process involves isolating DNA from an organism, introducing it into a vector or plasmid using restriction enzymes, and transforming it into a host cell. Restriction enzymes recognize specific DNA sequences and cleave the phosphodiester bonds between nucleotides at that site in viral, frog, or human DNA. Gene manipulation is concluded to include gene splicing, using recombinant DNA, forming monoclonal antibodies, and employing PCR thermocyclers, though it is not allowed for genetic manipulation in humans.
biotechnology and its applications
application s of biotechnology, bt.cotton, cloning, dna, dna fingerprinting, dna isolation, gene manipulation, genetic engineering, goldenrice., r dnatechnology, recombinant vaccines, transgenic, vectors
Economic upliftment through biotechnologymalinibindra
Bioengineering uses engineering principles and techniques to solve problems in biology and medicine. It has expanded beyond prosthetics and medical devices to include engineering at the molecular and cellular level with applications in energy, environment, and healthcare. Emerging biotechnologies include genetic modification, diagnostics, biopolymer chemistry, and environmental technologies. Conventional plant breeding and mutation breeding have improved crops for hundreds of years but cannot meet current global demands, so genetic engineering is being used to develop pest-resistant, drought-tolerant, and nutritionally enhanced crops.
Genetic engineering involves manipulating genetic material (DNA) to achieve desired goals. The basic principles involve artificially copying DNA from one organism and joining it into the DNA of another. Molecular tools like restriction enzymes and DNA ligases are used to cut and join DNA. Methods to transfer genes include transformation, electroporation, and liposome-mediated transfer. Applications include producing human proteins like insulin, developing gene therapies, and genetically modifying plants. Gene libraries, blotting techniques like Southern blotting, and PCR are also discussed as important molecular tools in genetic engineering.
Biotechnology has been used for millennia to improve agriculture, food production, and medicine through techniques like animal husbandry and fermentation. Modern biotechnology applies scientific principles to processing materials through biological agents. It has applications in medicine like drug development, agriculture like developing pest-resistant crops, and industry like producing chemicals. Biotechnology's scope continues expanding in fields such as genetic engineering, stem cell research, and environmental remediation.
1) The document discusses biosafety and bioethics issues related to microbial technology and biotechnology. It addresses concerns about genetically modified organisms (GMOs) and their impact on human health and the environment.
2) Good manufacturing practices (GMP) are guidelines that ensure products are consistently high quality and safe. They cover all aspects of production to minimize risks.
3) Proper rules and regulations around biosafety are important and vary depending on the organism and its intended use. Biosafety and gaining public trust are crucial to the development and application of biotechnology.
This document discusses several applications of biotechnology in medicine, including the production of human insulin, human growth hormone, vaccines, and gene therapy. It provides details on how recombinant DNA technology is used to produce these therapeutic products. Human insulin is extracted from pancreas cells and inserted into bacterial plasmids to be mass produced. Vaccines like hepatitis B vaccine involve isolating antigen genes and expressing them in yeast or bacteria. Gene therapy approaches like ex vivo therapy aim to correct genetic disorders by isolating cells, adding functional genes, and reinserting the cells.
This document discusses biotransformation, which is a chemical reaction catalyzed by living cells or enzymes. It can be used to modify the functional groups of organic compounds. The document outlines some prerequisites for successful biotransformation processes, including the culture having the necessary enzymes and the substrate not being toxic. It also discusses using plant cell and organ cultures, immobilized cell cultures, and genetic engineering approaches for biotransformation. Some factors that influence biotransformation are also summarized.
Gene cloning involves copying a gene and inserting it into a self-replicating vector to produce multiple copies of the gene. PCR (polymerase chain reaction) is a technique used to amplify specific genes. Both gene cloning and PCR are important for obtaining pure samples of genes and amplifying them for various applications like sequencing, expression of proteins, and genetic engineering.
The document discusses various applications of biotechnology including therapeutics, diagnostics, genetically modified crops for agriculture, and more. It also discusses three critical areas of biotechnology research: providing improved organisms as catalysts, creating optimal conditions for catalysts, and downstream processing technologies. The document then discusses options for increasing food production and how genetically modified crops are seen as a solution. It provides details on genetically modified organisms and Bt cotton as an example, describing how Bt genes provide insect resistance. The document also discusses using RNA interference to protect plants from parasites and the use of recombinant DNA technology in healthcare applications like producing insulin through E. coli. It covers gene therapy, PCR, ELISA, transgenic animals and their uses as well as
Genetic engineering is the process of manipulating genes to introduce desirable traits. It can be used to produce insulin and vaccines, treat genetic disorders through gene therapy or somatic cell gene therapy, and engineer plants and animals. Some applications include producing human growth hormone to treat dwarfism, making human albumin and anti-hemophilic factors, and developing GM crops with traits like pest resistance. However, critics argue that genetic engineering poses environmental and ethical risks by interfering with nature and potentially having irreversible effects.
Transgenic plants are plants that have been genetically modified using genetic engineering techniques to introduce new traits. The goal is to insert desirable genes from other organisms to produce crops with improved traits like pest or disease resistance, increased yield, or tolerance to environmental stresses. Some examples of transgenic crops include insect-resistant corn and cotton, herbicide-resistant soybeans, and golden rice which is enriched with vitamin A. While transgenic crops offer advantages to farmers and consumers, some concerns exist around their impact on human health, the environment, and traditional farming practices. Ongoing research continues to assess both the promises and risks of this emerging agricultural technology.
The document provides an overview of biotechnology, including definitions, key concepts, areas of application, and ethical considerations. It defines biotechnology as using living organisms to produce new products or modify existing organisms. Some main points covered include that biotechnology involves genetics, engineering, agriculture, and manipulating DNA. It also discusses early pioneers in the field and how techniques have advanced from classical to modern biotechnology.
Transformation is the process of altering an organism's genetic makeup by inserting new genes. Common transformation methods include Agrobacterium-mediated transformation, particle bombardment, protoplast transformation using polyethylene glycol or electroporation, and fibre-mediated DNA delivery. Agrobacterium transformation involves the bacteria transferring T-DNA from its Ti plasmid into the plant genome, while direct methods introduce naked DNA into plant cells using physical methods like particle bombardment or chemical treatments that make cell membranes permeable. Transformation allows improving crop traits like yield and stress resistance.
This is part of the MaRS BioEntrepreneurship series.
Speaker: Lynne Zydowsky, Ph.D., Managing Principal Zydowsky Consultants
* Explore the development of regulated drugs and devices
* Understand where and how value is generated in the pharmaceuticals industry
* Appreciate the interplay between science and business in a biotech company
To download a copy of the audio for this presentation, please go to:
http://www.marsdd.com/bioent/oct16
For the event blog and Q+A, please see:
http://blog.marsdd.com/2006/10/17/bringing-together-art-and-science/
The document discusses biobusiness and biosafety, providing definitions and opportunities for biotechnology in developing countries. It examines the market for biobusiness, key opportunity areas, and factors for successful bioenterprise innovation including focusing on high-value opportunities, recognizing that innovation need not have long life cycles, and emphasizing people over technologies. The document also outlines biosafety levels and concepts from containment to facility design to protect laboratory workers and the environment.
Single cell protein (SCP) refers to protein extracted from pure cultures of microorganisms like yeast, algae, fungi and bacteria. It can be used as a protein supplement for humans and animals. SCP is produced by growing microorganisms on substrates through fermentation. The microbes are then harvested, processed and treated to isolate and purify the protein. SCP has potential advantages as a sustainable protein source but also risks if toxic microbes or byproducts are consumed.
The document defines biotechnology as using living organisms to create useful products for humans. It discusses several types of biotechnology including plant biotechnology, which can be used for fruit development, vaccine production, and increasing nutritional quality. Animal biotechnology uses molecular biology techniques to genetically engineer animals for pharmaceutical, agricultural, or industrial applications. Food biotechnology produces the first genetically modified tomato that could be transported without bruising in the early 1990s. Industrial biotechnology uses cells and enzymes to generate industrial products and processes, while medical biotechnology researches and produces pharmaceuticals to treat and prevent diseases using cells and cell material.
This document discusses biotechnology, including its definition, history, applications in different fields like agriculture, medicine, and industry. It covers topics such as drug production using biotechnology techniques, pharmacogenomics, gene therapy, and genetic testing. Drug production through isolation and genetic engineering of enzymes is described. The use of biotechnology to develop medicines and pharmaceuticals for treating diseases is also summarized.
1. The seminar discusses developing transgenic plants resistant to insects through the transfer of resistance genes from microorganisms, higher plants, and animals into crop plants.
2. Major objectives of plant biotechnology are to develop plants resistant to biotic and abiotic stresses. Resistance to insects has been achieved by introducing genes encoding Bt toxins from Bacillus thuringiensis and other insecticidal proteins.
3. Useful genes have been isolated from microbes like B. thuringiensis, higher plants like beans and tobacco, and animals like mammals. These genes have been successfully used to engineer insect-resistant crops like cotton, potato, tomato, and tobacco.
Gene manipulation involves externally arranging genes within an organism and is also known as genetic engineering. It was first used in agriculture to improve plant quality and was discovered in native Mexican corn in 2001. The process involves isolating DNA from an organism, introducing it into a vector or plasmid using restriction enzymes, and transforming it into a host cell. Restriction enzymes recognize specific DNA sequences and cleave the phosphodiester bonds between nucleotides at that site in viral, frog, or human DNA. Gene manipulation is concluded to include gene splicing, using recombinant DNA, forming monoclonal antibodies, and employing PCR thermocyclers, though it is not allowed for genetic manipulation in humans.
biotechnology and its applications
application s of biotechnology, bt.cotton, cloning, dna, dna fingerprinting, dna isolation, gene manipulation, genetic engineering, goldenrice., r dnatechnology, recombinant vaccines, transgenic, vectors
Economic upliftment through biotechnologymalinibindra
Bioengineering uses engineering principles and techniques to solve problems in biology and medicine. It has expanded beyond prosthetics and medical devices to include engineering at the molecular and cellular level with applications in energy, environment, and healthcare. Emerging biotechnologies include genetic modification, diagnostics, biopolymer chemistry, and environmental technologies. Conventional plant breeding and mutation breeding have improved crops for hundreds of years but cannot meet current global demands, so genetic engineering is being used to develop pest-resistant, drought-tolerant, and nutritionally enhanced crops.
This document contains definitions and information about genetic engineering, DNA fingerprinting, clones, and the human genome from various websites. It defines genetic engineering as changes made to DNA to change an organism, DNA fingerprinting as the analysis of DNA samples to identify individuals, clones as identical copies of an organism, and the human genome as the total number of genes found in humans, which is estimated to be between 20,000 and 25,000 genes. The document provides short explanations and cites multiple online sources for each term.
Biotechnology ppt by anila rani pullaguraanilarani
Biotechnology is the use of living systems and organisms to develop or make products, or "any technological application that uses biological systems, living organisms or derivatives thereof, to make or modify products or processes for specific use"
This document provides an overview of biology as an academic subject. It begins by defining biology as the study of living things, including their structure, life processes, characteristics, and interactions with the environment. It outlines several key characteristics of living things and discusses the importance of studying biology to understand ourselves, diseases, and find new treatments. The document also describes several major fields of study within biology, including areas focused on structure and function, grouping of organisms, development, environments, and applied fields. It concludes by listing various careers that are related to the study of biology.
This document provides an introduction to biotechnology, describing it as using scientific processes to develop new organisms or products from organisms to meet human needs like food, clothing, shelter, health and safety. It discusses areas of biotechnology like agriculture, medicine, environment, and food/beverage processing. Agricultural biotechnology focuses on improving plants and animals for food production through techniques like genetic engineering and cloning, while medical biotechnology develops therapies and pharmaceuticals. The document outlines the goals and applications of biotechnology in various industries.
Biotechnology is the use of living organisms to develop useful products. Modern biotechnology techniques include isolating DNA, inserting genes into vectors, and transforming host cells. Applications include producing Bt crops for pest resistance, producing vaccines and diagnosing diseases. New advances include artificial lymph nodes, non-invasive cancer detection from saliva, smart contact lenses to monitor eye pressure, and machines that can scan the liver non-invasively. Biotechnology continues to progress rapidly with applications in agriculture, medicine, and other fields.
Application of Biotechnology In Medicine By Anila Rani Pullaguraanilarani
Biotechnology is a very huge field and its applications are used in a variety of fields of science such as agriculture and medicine. Medicine is by means of biotechnology techniques so much in diagnosing and treating dissimilar diseases. It also gives opportunity for the populace to defend themselves from hazardous diseases.
Biotechnology can be applied to waste management through microbial fuel cells (MFCs). MFCs use microorganisms to convert the chemical energy in organic compounds into electrical energy. They have two chambers, an anode where microbes in the wastewater oxidize organic matter and release electrons and protons, and a cathode where oxygen reacts with the electrons and protons to form water. This generates a current that can be used as energy. The document describes a student's experiment using an MFC with effluent water, which generated voltages of up to 120mV over 5 days. MFCs provide a way to both treat wastewater and produce renewable energy, though further improvements are still needed.
B sc biotech i fob unit 1 introduction to biotechnologyRai University
This document provides an overview of biotechnology. It defines biotechnology as using living organisms to make useful products. Biotechnology draws on fields like microbiology, biochemistry, and molecular biology. It has applications in healthcare, agriculture, industry, and the environment. The document also discusses biosafety considerations and ensuring public acceptance of biotechnology applications.
This document provides an introduction to biotechnology. It defines biotechnology as using scientific methods to produce new products or organisms. It then discusses how biotechnology is applied in various fields like agriculture, medicine, environment, and food processing. Some key applications mentioned are using biotechnology to improve milk production, develop disease-resistant plants, conduct gene therapy, produce renewable energy, and develop health-promoting or nutrient-enriched foods. The document emphasizes that biotechnology helps meet basic human needs while enhancing safety, efficiency and productivity.
This document provides an introduction and overview of biotechnology, including definitions of key terms and an historical timeline of important developments in the field. It begins with definitions of biotechnology and genetic engineering. It then outlines the timeline of biotechnology from early domestication and farming in Mesopotamia through modern developments like recombinant vaccines, cloning, and the human genome project. The document concludes with a note about an upcoming meeting to level off on the material.
Biotechnology is defined as any technique that uses living organisms or substances from those organisms to make or modify a product, to improve plants or animals, or to develop microorganisms for specific uses. It helps meet basic human needs like food, clothing, shelter, health and safety through scientific advances in agriculture, medicine, and environmental management. Some key applications of biotechnology include using enzymes in detergents and the pulp and paper industry, genetically engineering crops for traits like insect or drought resistance, and developing pharmaceuticals through biotechnology techniques like genetic engineering and cell culture.
This document discusses the application of biotechnology in the food, pharmaceutical, and agriculture industries. It provides examples of how biotechnology is used in food processing, such as developing new emulsifiers and tests for food allergens. In pharmaceuticals, biotechnology has been used to develop vaccines, insulin, blood products, and gene therapies. In agriculture, biotechnology can be applied to increase pest resistance, disease resistance, nutritional quality, and environmental stress tolerance in crops. Genetically modified crops are also discussed.
Application of Biotechnology in different fieldsVinod Kumar
This document provides an overview of the application of biotechnology in different fields including food, medical, agriculture, and environmental biotechnology. Some key points:
- Food biotechnology is used to genetically modify plants and animals for improved production, shelf life, nutrient composition, and drug delivery. Examples given are tomatoes with longer shelf life and golden rice engineered to produce vitamin A.
- Medical biotechnology aims to prolong life through technologies like monoclonal antibodies to treat cancer, bioprocessing insulin from bacteria, stem cells for tissue regeneration, and tissue engineering of organs.
- Agriculture biotechnology is applied through plant tissue culture to develop transgenic crops with desired traits like pest and stress resistance.
- Environmental biotechnology addresses
The document provides an overview of the field of biotechnology, including its history, key areas and applications. It discusses topics like genetic engineering, recombinant DNA technology, transgenic plants and animals, DNA microarrays, bioinformatics, and careers in biotechnology. The future prospects of biotechnology in addressing global challenges like food security and healthcare are also highlighted.
Biotechnology is the use of living organisms and their products for health, social, or economic purposes through various technologies applied to living cells and genes. It involves fields like microbiology, biochemistry, genetics, and engineering. Key developments include DNA structure discovery enabling gene cloning and genetic engineering. This allows desirable changes to organisms' genes, like combining animal genes into plants. Genetically modified foods are now common, with little evidence of health issues. Cloning led to Dolly the sheep, and chips may one day diagnose diseases. Biotechnology has wide applications in agriculture, industry, medicine, and more.
With the advancement of biotechnology, Genetic engineering also become an important tool. Transgenic crops are the crops which are produced through genetic engineering by altering desirable traits into plant genome.
Plant biotechnology is the application of scientific techniques to modify and manipulate plant cells or tissues for beneficial uses. Some key techniques include genetic engineering, which involves transferring genes between organisms, and tissue culture, which involves growing plant cells or tissues in artificial conditions. The first transgenic plant was created in 1983 when scientists transferred genes from other animals into tobacco plants. Golden rice, developed in 1999, was one of the earliest genetically engineered crops and was intended to produce beta-carotene to address vitamin A deficiencies.
This document discusses the stages and applications of biotechnology. It begins by outlining the three stages of biotechnology development: ancient biotechnology related to food and shelter, classical biotechnology using fermentation for food production and medicine, and modern biotechnology using genetic engineering techniques. It then states that bacteria are useful in biotechnology due to their rapid reproduction and ability to produce complex molecules. The document goes on to discuss areas of biotechnology like genetic engineering, protein engineering, and plant/animal biotechnology. It provides examples of genetic engineering applications including producing recombinant proteins, transgenic plants and animals, animal cloning, and gene therapy to cure hereditary diseases.
This presentation highlights some important facts about biotechnology in relationship to plants. it lay emphasis on some factors associated with biotechnology, the importance of it and the negative impact as well.
Genetic engineering has many applications in medicine and industry. In medicine, it is used for gene mapping, treatment of genetic disorders, production of monoclonal antibodies, gene therapy, DNA fingerprinting, and creation of vaccines and pharmaceutical products. In industry, genetic engineering has applications in protein engineering, metabolic engineering, the pharmaceutical, bioplastics, oil, mining, fuel, and other industries. It allows manipulation of genes to produce desired proteins, enzymes, organisms, or traits.
This document provides an overview of genetic engineering and its applications to microorganisms. It defines genetic engineering as the direct manipulation of an organism's genome using biotechnology. The key steps involved are isolating the gene of interest, inserting it into a vector, introducing the vector into a host cell, and harvesting the gene product from the clone. Common hosts used are bacteria, yeast, plant and animal cells. The document also discusses some tools used in genetic engineering like restriction enzymes and DNA ligase. It outlines several applications of genetic engineering in medicine, research, agriculture and industry. It concludes by noting some ethical and safety concerns regarding genetically modified organisms.
Plant Genetic engineering ,Basic steps ,Advantages and disadvantagesTessaRaju
plant genetic engineering,first genetically engineered crop plant,first genetically engineered foods,genome editing,uses of GE,transgenic plants,basic process of plant genetic enginering,advantages and disadvantages of genetic engineering.
This document discusses applications of genetic technologies including recombinant protein production, transgenic plants, and transgenic animals. Recombinant DNA technology is used to produce proteins like insulin on a large scale by inserting genes into bacteria or other hosts. Transgenic plants are created by inserting foreign genes to improve traits like disease resistance or nutritional quality. Transgenic animals carry inserted genes to increase growth rates, improve disease resistance, or produce recombinant proteins in milk. Examples of transgenic crops and animals are also provided.
Biotechnology can be summarized as follows:
1. It uses living organisms or their components to develop useful products. This includes genetically modifying microbes, plants and animals.
2. Key branches include genetic engineering, tissue culture, DNA fingerprinting and gene therapy. Genetic engineering is used to create GMOs by transferring genes between organisms.
3. Important tools for genetic engineering include vectors, restriction enzymes, DNA ligases, and host cells used to replicate recombinant DNA. This allows genes of interest to be isolated and transferred to create GMOs.
Biotechnology is the application of living organisms to modify products or develop microorganisms for specific uses. Genetic engineering uses recombinant DNA technology to transfer genes between organisms, producing transgenic organisms. Key tools include vectors to transport genes, restriction enzymes to cut DNA, and DNA ligase to join DNA fragments. Recombinant DNA technology involves cloning genes by inserting them into host cells like E.coli to produce copies. Insulin was the first protein produced using this method. DNA fingerprinting identifies individuals by analyzing variable number tandem repeats in genomic DNA.
Concept of genetic engineering Nar Bahadur Khatrinaru khatri
Genetic engineering, also known as recombinant DNA technology, is the process of manipulating genes to obtain desired traits. It involves adding, deleting, or replacing genetic material in an organism. Key tools for genetic engineering include restriction enzymes, DNA ligase, DNA polymerase, and vectors. The process involves isolating a target gene, inserting it into a vector, transferring the recombinant DNA into a host, and cloning the target gene to produce many identical copies. Genetic engineering has applications in medicine production, transgenic organisms, DNA fingerprinting, and environmental remediation. However, there are also health and environmental risks to consider.
Biotechnological aspects of product developmentNaveed Sarwar
This document discusses biotechnological product development. It explains that biotechnology uses biological systems to develop technologies and products for improving quality of life. Biotech drugs, also called biopharmaceuticals, are manufactured using living expression systems and differ physically from small molecule drugs. The development process involves isolating a gene of interest, transferring it to an expression vector, transforming host cells, growing the cells, and purifying the protein product. Quality control measures like sterility testing and viral/bacterial decontamination are crucial given biotech drugs cannot be sterilized as end products.
This document provides an overview of biotechnology. It discusses what biotechnology is, which includes using biological sciences like agriculture, food science, genetics, and medicine to develop new products. It also discusses areas of biotechnology like genetic engineering, protein engineering, bioinformatics, immunology, plant biotechnology, and cancer biology. Key techniques in biotechnology are also summarized like recombinant DNA technology, polymerase chain reaction, DNA microarrays, genetic modification of plants, monoclonal antibodies, and applications of nanobiotechnology.
This document discusses various applications of biotechnology including therapeutics, diagnostics, food production, environmental applications, agriculture, and chemical production. It also describes techniques such as genetic engineering, DNA probes, DNA sequencing, PCR, gene therapy, biosensors, biochips, nanoparticles, assisted reproductive technologies, vaccine development, hybridoma technology, stem cell technology, and fermentation technology. The key applications and components of these techniques are summarized.
Genetic engineering principle, tools, techniques, types and applicationTarun Kapoor
Basic principles of genetic engineering.
Study of cloning vectors, restriction endonucleases and DNA ligase.
Recombinant DNA technology. Application of genetic engineering in medicine.
Application of r DNA technology and genetic engineering in the products:
a. Interferon
b. Vaccines- hepatitis- B
c. Hormones- Insulin.
Polymerase chain reaction
Brief introduction to PCR
Basic principles of PCR
Genetic engineering involves directly manipulating an organism's genome using biotechnology. The process involves isolating a gene, cutting it using restriction enzymes, inserting it into a plasmid or other vector, and introducing this recombinant DNA into a host organism where it is expressed. Key applications of genetic engineering include agriculture, where genes are inserted into crops to make them pest-resistant or improve yields, and medicine, where genes or cells are modified to produce vaccines, hormones, or treat genetic diseases through gene therapy or gene pharming.
1 r dna & its pharmaceutical applications prasanthi rao
Recombinant DNA technology involves isolating a gene of interest and inserting it into a plasmid or bacterial chromosome. The modified bacteria can then be used to produce therapeutic proteins, vaccines, diagnose and screen for genetic diseases, and conduct gene therapy and DNA fingerprinting. Agricultural applications include creating herbicide-tolerant, pest-resistant, drought-tolerant and nutritionally enhanced crops. Environmental studies use recombinant DNA to identify microbes and study their roles.
To achieve genetic transformation in plants, we need the construction of a vector (genetic vehicle) which transports the genes of interest, flanked by the necessary controlling sequences i.e. promoter and terminator, and deliver the genes into the host plant.
Recombinant Protein Technology in laboratory .pptxPavel ( NSTU)
Recombinant protein technology is a set of techniques used to produce proteins by genetically modifying organisms. This technology is widely used in research, medicine, and industry for producing proteins that are difficult to isolate in large quantities from natural sources.
Recombinant protein technology is of paramount importance across various fields due to its ability to produce specific proteins in large quantities, with high purity and consistency.Recombinant protein technology is a cornerstone of modern biotechnology, with wide-ranging applications that impact healthcare, research, industry, and agriculture. Its ability to produce high-quality proteins efficiently and safely makes it an indispensable tool in addressing many of the challenges faced by society today.
Chapter 5 principles of inheritance and variationmohan bio
- Mendelian genetics deals with the study of heredity and variation through experiments in pea plants by Gregor Mendel.
- Mendel discovered the laws of inheritance through experiments showing traits are inherited in dominant and recessive patterns.
- His work was later combined with the chromosomal theory of inheritance which showed genes are located on chromosomes and segregate during gamete formation according to Mendel's laws.
Chapter 6. Molecular basis of inheritance.mohan bio
Nucleic acids like DNA and RNA are the genetic material found in living cells. DNA carries genetic information from one generation to the next and is made up of deoxyribose, phosphate groups, and nitrogenous bases. DNA replication is semi-conservative and produces two identical DNA molecules, each with one old and one new strand. Transcription produces mRNA from a DNA template, and translation reads mRNA to produce proteins according to the central dogma of biology.
This document discusses human health and diseases. It defines health and discusses factors that affect health like genetics, lifestyle, and infectious/non-infectious diseases. It then summarizes several common infectious diseases like typhoid, pneumonia, malaria, and their causes, transmission methods, symptoms, and treatment. It also discusses immunity, describing innate and acquired immunity. Innate immunity includes physical and chemical barriers, while acquired immunity involves T cells, B cells, antibodies, and cellular/humoral responses that provide long-term protection against pathogens.
The document discusses reproductive health issues in India. It covers topics like early marriage, lack of knowledge about reproductive health leading to high maternal and infant mortality rates, and population explosion due to lack of family planning programs. It describes various contraceptive methods like natural family planning, barrier methods, IUDs, oral contraceptives, and sterilization. It also discusses infertility treatment methods, sexually transmitted diseases, and strategies to improve awareness about reproductive health issues through various government programs.
2. • The application of technology to improve a
biological organism.
natural variation: Allelic differences at genes
control a specific trait.
• Gene - a piece of DNA that controls the
expression of a trait.
• Allele - the alternate forms of a gene.
3. • Central Dogma of Molecular Genetics
DNA
RNA
Transcription
Translation
Protein
trait or phenotype.
• The application of the technology to modify the
biological function of an organism by adding
genes from another organism.
4. Biotechnology.
• Utilization of biological entities
and their component in
production of some products for
human welfare is called
biotechnology.
• The contribution of biotechnology
in different field of biology are,
5.
6. Medical Biotechnology:
• Production of human insulin using recombinant
DNA technology.
• Production of anti biotic like penicillin
erythromycin. Etc.
• Production of mono clonal antibodies using
hybridoma technology.
• Treating defective gene using gene therapy.
• Identification of immigrants. Criminals, disputed
parents, missing baby etc. using DNA finger
printing technology.
7.
8. Environmental biotechnology.
• Some microbes are used to treat sewage
waste in water purification.
• Detoxification of industrial waste are done
using microbes.
• Some microbes are used to reduce the
percentage of oxides of sulphur in industrial
effluents.
• Degradation of petroleum products and
management of oil spills are done by using
microbes.
9.
10. Industrial biotechnology.
• Production of useful organic compounds like
ethyl alcohol, lactic acid, citric acid etc. by using
microbes.
• Production of enzymes like amylase, lipase,
protease from microbes.
• Production of bio fuel like ethanol, bio gas etc.
• extraction of some minerals like copper,
uranium, from low grade ore using microbes.
11.
12. Plant biotechnology or agricultural
biotechnology.
• Rapid multiplication of crop plants, medicinal
plants, forest plants and endangered plants
using tissue culture.
• Production of viral and other pathogen
resistance plants.
• Production of haploid or polyploidy crop
plants to increase yield.
• Production of transgenic plants as nitrogen
fixing plants, insect resistance plants etc.
13.
14. Animal biotechnology
• To develop Genetically modified animals or transgenic
animals.
• Transgenic cows – increase milk supply and meat
• Transgenic Chickens – more resistant to infections.
• Transgenic Goats, sheep and pigs – produce human
proteins in their milk
• To increase the heard of specific breed using invitro
fertilization and embryo transfer.
• Cloning of animals.
• Transgenic mice – used to study human immune system
• NTBT: National biotechnology board.
• DBT: department of biotechnology.
15. • Application of biotechnology varies from agriculture to
industry - food, pharmaceutical, chemical, bio-products,
textiles, medicine, nutrition, environmental conservation,
animal sciences etc.
• Admission to the integrated five year M.Tech program
offered by IIT Delhi and Kharagpur is through the Joint
Entrance Exam (JEE).
• Jawaharlal Nehru University, New Delhi conducts a
combined all India level entrance examination for MSc
Biotechnology program.
• Candidates with Bachelor's degree under 10+2+3 pattern
of education in Physical, Biological, Agricultural, Veterinary
& Fishery Sciences, Pharmacy, Engineering, Technology 4Years BS (Physician Assistant Course); OR Medicine (MBBS)
OR BDS with at least 55% marks are eligible to apply for
MSc (Biotechnology) offered by JNU and several other
universities all over the country
16. A biotechnologist may find jobs in various
quarters. In India Students can mainly explore
job options in the following fields:
• Drug and pharmaceutical research
• Public funded laboratories
• Chemicals
• Environment control
• Waste management
• Energy
• Food processing
• Bio-processing industries
17. • The government institutes and organizations, such as
Department of Biotechnology (DBT), several
agriculture, dairy and horticulture institutes offer
employment.
• In private sector, Drug companies in biotechnology
like Dabur, Ranbaxy, Hindustan Lever, Dr Reddy's Labs
that have their R & D units offer Biotechnology
professional .
• Even in the food processing industry, chemical
industry and the textile industry.
• The major companies, which hire biotechnologists,
are Hindustan Lever, Thapar Group, Indo American
Hybrid Seeds, Bincon India Ltd., IDPL and Hindustan
Antibiotics etc.
18. • Institutes Offering B.Tech/M.Tech/PhD :
• Admission to the integrated five year M.Tech
program offered by IIT Delhi and Kharagpur is
through the Joint Entrance Exam (JEE).
• Indian Institute of Technology, Delhi
• Courses Offered: B.Tech., M.Tech. in
Biochemical Engineering and Biotechnology,
M.S. (Research) in Biochemical Engineering and
Biotechnology and Pre Ph.D. Courses
• National Dairy Research Institute, Karnal
• Courses Offered : M.Sc and M.Tech degree in
Animal Biotechnology
19. • Indian Institute of Technology, kharagpur.
• Courses Offered : B.Tech.(H) in Biotechnology
and Biochemical Engineering, B.Tech.(H) and
M.Tech. in Biotechnology and Biochemical
Engineering, MS (Biotechnology), M.Tech. Biotechnology and Biochemical Engineering,
Ph.D. (Biotechnology)
• All India Institute of Medical Sciences (AIIMS)
• Courses Offered : M Biotech
20. • Centre for Cellular and Molecular Biology
imparts training to doctoral students in an
academic program linked to the Jawaharlal
Nehru University, New Delhi. Besides, the
Centre also trains post-doctoral fellows though
training programs sponsored by CSIR,
Department of Biotechnology (DBT), and the
Department of Science and Technology (DST),
Govt. of India, New Delhi.
• On an average at any given point of time there
are over 100 such researchers at the CCMB,
including guest workers from various
institutions.
21. • The students enrolled in academic programs
require to have strong motivation to pursue
research in modern biology leading to a Ph.D
degree.
• The projects offered for Ph.D. cover
specialized areas of Cell Biology, Molecular
Biology, Genetics, Genomics, Developmental
Biology, Nano biology, Plant Molecular
Biology, Membrane Biology, Protein Structure
and Function, Biology
• of Macromolecules, Biology of Infection,
Epigenetics, Chromatin Biology and
Bioinformatics
22.
23. Genetic Engineering (Gene Manipulation )
• The technique of transferring desired gene to an
organism to manipulate its genome is called genetic
engineering.
Application of Genetic engineering:
• Understanding biological events in biological courses.
• Production of pharmaceutical compounds like
insulin, growth hormone,etc
• Production of transgenic animals.
• Production of transgenic plants.
• Production of pathogen and insect resistance plants.
24. Tools used in Genetic Engineering.
•
•
•
•
•
Desired gene
Vector
Enzymes: REN, DNA ligase.
Host cell.
Bio reactor.
25. • Desired gene: The functional or normal gene of
our interest taken from donor cell.
It is also known as foreign gene or trans gene.
• Vector: The carrier DNA that act as vehicle to
carry desired gene to the host cell is called vector.
• The imp vectors used in Genetic engineering are,
1. Plasmid.
2. Phages.
3. Plant virus.
4. Animal virus.
5. Cosmids.
6. Artificial chromosomes.
26. • Plasmids: The extra chromosomal small circular
self replicating DNA present in bacterial cell is
called plasmid. The number of plasmid varies
from 1 to 20 in a single bacterial cell.
27. Types of plasmids:
• F+ plasmid: It is the plasmid that contains
fertility factor.
• R plasmid: It is the plasmid that contains
antibiotic resistance gene.
Ex: ampicillin and tetracycline resistance
gene.
• Col plasmid: it is the plasmid that contains col
gene that synthesizes the protein colocin. The
colocin kills the other strains of bacteria.
28. • Virulence plasmid: It is the plasmid that
contains pathogenic gene.
• Metabolic plasmid: It is the plasmid that
contains gene for metabolic activity.
–Ex: nif + gene.
Common plasmids used in genetic
engineering:
• pBR 322.
• pUC18.
• Ti plasmid. ( tumor inducing plasmid)
• Ri plasmid. ( root inducing plasmid)
29.
30. pBR 322 plasmid:
• It is the naturally occurring E. coli plasmid.
• It has 4.3 Kbs. (kilo base pair size)
• It contains one Ori site. ( origin of replication
site).
• It contains two antibiotic resistance genes.
Amp+ and Tet +
• It contains specific restriction endonuclease
recognizing site.
31.
32.
33. pUC 18 plasmid.
• pUC 18 was first constructed at
university of California.
• It has the size of 2.73 kbs
• It contains one ori site.
• The fertility factor is absent.
• It contains ampicillin resistance
gene.
• It contains Lac promoter and
lac Z gene.
• The lac Z gene it contains 10 to
15 restricted sites for different
REN. It is called MCS ( multiple
cloning site.)
34. • multiple cloning site. (MCS): 10 to 15
restricted sites for different REN present in lac
Z gene of pUC 18 plasmid is called MCS.
Enzymes in genetic engineering:
• The two imp enzymes used as molecular
scissor and molecular stitchers are Restricted
endonuclease enzyme (REN) and DNA ligase.
35. Restricted endonuclease enzyme
• REN is the endonuclease enzyme that cuts
double stranded DNA molecule at specific
palindrome sequence. It is Used as a molecular
scissor in genetic engineering.
• REN are the defensive enzyme for bacteria. It
cuts and destroys bacteriophage DNA that infects
bacterial cell.
• Different types of REN are identified and isolated
for different palindromic sequence.
36. • Hamilton smith discovered and isolated
HIND II REN from Haemophilius influenzae in
1968. He received the Nobel Prize in
Physiology or Medicine in 1978.
37. palindromic sequence
• The region of DNA in which two strands are
identical when read in both the direction is
called palindromic sequence.
Ex: palindromic sequence for Eco-I is.
38. • palindromic sequence for HIND III is
5'-A |A G C T T-3'
3'-T T C G A| A-5‘
• In bacteria specific DNA palindromic sequence
are methylated periodically throughout the
genome. Hence REN is not effective against
bacterial genome.
• Foreign DNAs which are not methylated are
introduced into the cell are degraded by
sequence-specific restriction enzymes and
cleaved.
39.
40. DNA ligase.
• The enzyme that joins the two sticky
ends of DNA is called DNA ligase. It is
used as molecular sitichers in genetic
engineering.
• DNA ligase was discovered by H G
Khorna.
• Dr. Hargobind Khorana was born on
9th January 1922 at Raipur, Punjab
(now in Pakistan).
• Died
November 9, 2011 (aged
89)Concord, Massachusetts, U.S.
• In 1968, He was awarded the Nobel
Prize in Physiology or Medicine for the
interpretation of the genetic code and
its function in protein synthesis
1922 - 2011
41. • Host cell: The cell to which desired new gene is
introduced is called host cell. Any living cell can be
used as host cell. Commonly E.coli bacterial cell is
used as host cell in genetic engineering. Because,
1. It is a simple prokaryotic cell.
2. It is a non-pathogenic bacteria.
3. It can be cultured easily in laboratory condition.
4. It has very short life span.
5. It contains self replicating plasmid.
6. The plasmid of E.coli can be easily handled as
vector.
42. Bioreactor
• It is an apparatus for culturing organisms like
algae, fungi, bacteria, or animal or plant cells
under controlled conditions.
• It is used in industrial processes to produce
pharmaceuticals, vaccines, or antibodies.
• It give the cells a homogeneous and controlled
environment by ensuring the same temperature,
pH, and oxygen levels.
46. • Bioreactor consists of
vessel which holds the
media and the cells. It can
be made of glass,
stainless steel, or a
durable plastic.
• An agitator or stirrer is
fixed inside to mix the
contents in the vessel.
Mixing of the contents is
to maintain a constant
nutrients and oxygen to
the culture.
47. • The sparger is an apparatus used
to introduce gasses into the
vessel. It aerate and supply
oxygen to the contents in the
vessel, as well as to the cells.
• Bioreactors has inlets to monitor
the culture in the vessel. Useful
inlets are foam control system
and pH control
• Cooling jacket with water
circulation maintains the
temperature.
• It contains additional ports to
introduce and remove materials
from vessels. The outlet is
present at bottom to collect
product.
48. Application of bioreactor.
• It is used to culture microbes like bacteria, fungi,
algae or plant cell or animal cell.
• It is used for the production of single cell protein.
• It is used for culturing genetically modified
microbes for production of antibiotics,
pharmaceutical compounds, vaccines etc.
• It is in the production of primary metabolites
from microbes.
49. Recombinant DNA technology:
• The technology of incorporation of desired gene
to the vector DNA and transferring it into host cell
is called r-DNA technology.
Steps involved in r-DNA technology:
1. Extraction of DNA or isolation of gene.
2. Selection of vector.
3. Gene splicing.
4. Transfer of r-DNA to the host cell.
5. Culturing of transformed host cell.
50.
51.
52. Extraction of DNA or isolation of gene:
• The cells of organism that
contains desired gene are
collected.
• The DNA of these cells is
extracted by using
refrigerated centrifuge
technology.
• The isolation of gene is done
by shoot gun method. In this
specific REN is used to cut
and isolate desired gene. The
isolated gene contains two
sticky ends.
53. • Complementary
DNA ( c-DNA)
• c-DNA is used
instead of isolating
desired gene. In
this m-RNA is
transcribed from
desired gene is
used as templet to
syntheses of DNA
using reverse
transcription.
54. • The single stranded DNA is later converted
into double stranded DNA.
• The DNA synthesized by reverse transcription
of m-RNA using reverse transcriptase enzyme
is called c-DNA.
• Artificial gene: The DNA synthesized with
reference to the number and sequence of
amino acids of protein chain in laboratory
condition is called artificial gene
55. • Multiplication of gene: The isolated desired
gene is multiplied into millions of copies using
polymerase chain reaction.
56. Selection of vector:
• Vector is a vehicle that carries
desired gene into host cell.
Depending on host cell vectors
like plasmids or phages are
selected.
Gene splicing:
• Incorporation of desired gene
into vector to develop r-DNA is
called gene splicing.
• The REN is used to cut the vector
at specific restricted site to insert
desired gene. Later it is ligated by
DNA ligase.
• The vector with desired gene is
called r-DNA.
57. Transfer of r-DNA into host cell:
• The bacterial cell ( host cell) and r-DNA are
made to suspend in cold (5-6 0C ) calcium
chloride solution. After some interval of time,
the temp of solution is suddenly raised to 42
0C and again cooled.
• The increase in temperature increases the
pore size of bacterial membrane. Through this
pore r-DNA enters the bacterial cell .
58. Culturing of transformed host cell:
• The transformed host cells are screened with
antibiotic to select r-DNA transformed cells.
These cells are isolated and cultured in
bioreactor.
59. Human insulin
• Insulin is a protein natured hormone that
maintains sugar metabolism. It converts the
excess of blood sugar (glucose) into glycogen
to maintain normal sugar level.
• This hormone is secreted by β-cells of islets of
Langerhans present in pancreas.
• The deficiency of insulin increases the blood
sugar level and causes diabetes mellitus.
60.
61.
62. • The diabetic patients are treated with
hypoglycemic oral drug or insulin injection.
• The oral drug stimulates the β–cells to secrete
insulin.
• In previous years insulin extracted from cows
and pigs are injected to control diabetic
condition. It causes allergy to most of the
patients.
• The r-DNA technology gives solution to
overcome this problem by producing human
insulin (humulin) using human insulin
producing gene.
63.
64. Production of human insulin by r-DNA
technology.
•
•
•
•
•
•
•
Tools required.
Proinsulin gene.
vector pUC 18.
REN HIND – III
DNA ligase.
Host cell- E.coli.
Bioreactor.
65. • m- RNA of proinsulin is used to produce
complimentary DNA by reverse transcription
process.
• The m-RNA of proinsulin is treated with
reverse transcriptase enzyme and deoxyribo
nucleotide to get c-DNA.
• Single stranded c-DNA hybridized to get
double stranded c-DNA.
• The c-DNA of proinsulin is incorporated with
pUC18 with in lac Z gene using REN HIND III
and DNA ligase.
66. • r-DNA and host cells E.coli are made to are
suspend in cold (5-6 0C ) calcium chloride
solution. After some interval of time, the temp of
solution is suddenly raised to 42 0C and again
cooled.
• The increase in temperature increases the pore
size of bacterial membrane. Through this pore rDNA enters the bacterial cell.
• The E.coli are screened to ampicillin to isolate
transformed cells.
• The transformed E.coli are cultured in bioreactor
to produce proinsulin.
• The transformed E.coli produces proinsulin along
with β–galactosidase.
67. • The fused proinsulin from β-galactosidase is
isolated by treating with cyanobromide
(CNBr).
• The proinsulin is inactive form and contains α,
β and c chain.
• It is treated with proteiolytic enzymes trypsin
and carboxy peptidase to remove c-chain
• The product obtained is functional insulin
having α and β - chains bounded by two disulphide bond.
68. Application of r-DNA technology.
1.
2.
3.
4.
5.
6.
7.
8.
9.
In production of human insulin to treat diabetes mellitus.
In production of growth hormone to treat dwarfism.
In production of blood clotting factor VIII to treat hemophilia.
In production of interferon's to treat viral disease and cancer.
In production of vitamins, enzymes, amino acids for
commercial use.
In production of alcohol.
In production of GMO plants as golden rice, BT plants, insect
resistance, viral resistance, plants.
In production of GMO microbes to clean environment
pollutant.
In production of GMO microbes to extract metals from low
grade ore.
69.
70. DNA finger printing technology.
• The technology used for
identification of individual
at genetic level is called
DNA finger printing
technology.
• This technology was first
developed by alec
Jeffreys, Wilson and Thein
in 1985.
Born 9 January
1950 (age 62)
Oxford, United
Kingdom
71. • The principle is based on matching of VNTRs of
DNA collected at crime spot with suspect person
DNA.
• VNTRs: Variable number of tandem repeats. It is
also called as mini satellites.
• The identical and repeated sequence of
nucleotides present adjacent to each other in DNA
is called VNTRs.
• VNTRs are very specific to individual and differs
from person to person. It shows some similarities
between family members.
• VNTRs of identical twins are same. Hence it is not
possible to identify individuality in identical twins
by DNA finger printing technology.
72. Application of DNA finger printing
technology.
1.
2.
3.
4.
5.
It is used to identify criminals and rapist.
To solve parental dispute.
To solve immigrant problems.
To identify dead bodies of soldiers died in wars.
To identify dead bodies of person died at
accidents and bomb blast.
6. To identify racial groups.
7. To detect inheritable disorders.
8. To detect donor cell in case of transplantation.
74. • The DNA is isolated from the sample of blood
cells, hair root cells, semen or bone collected at
crime spot.
• The DNA of suspect also collected and isolated
separately.
• The isolated DNA is treated with REN to cut into
number of fragments.
• The DNA fragments are separated according to
their length on gel slab using gel electrophoresis.
• The DNA strand on gel slab is treated with
alkaline solution to split double strand in to single
strand.
75. • The single strand DNA is transferred to nylon
sheath using southern blotting technology.
• The single stranded DNA is hybridized with
radioactive probes of VNTRs . The excess of
probes are washed off.
• Nylon sheath is X-ray photographed to get
bands of VNTRs.
• The bands of X-ray sheath is the DNA finger
print.
• Comparing the DNA finger print of sample
collected at crime spot with suspect identifies
the individuality.
76. • Southern blotting: The technique of
transferring DNA from agar gel to
nylon sheath is called southern
blotting.
• Probe: Single stranded
polynucleotide fragment
complementary to specific sequence
of nucleotides of DNA is called
probe. It is mainly used in identify
VNTRs and desired gene
77. Gene therapy
• The technique of replacement of defective gene
by normal functional gene to treat genetic
disorder is called gene therapy.
Two ways of gene therapy:
1. Invivo approach.
2. Invitro approach.
• In invivo approach normal functional gene is
directly transferred to the target organ of patient.
• In invitro approach the defective cells are
cultured in lab condition. The normal gene is
transferred to this cultured cell.
The genetically modified cell or tissue is
transplanted to patient.
78. The disease cured by gene therapy;
1. SCID: Sevier combined immune deficiency
syndrome.
2. Cystic fibrosis.
3. Muscular dystrophy.
Types of gene therapy:
• Somatic gene therapy: It is the replacement of
defective gene by normal gene to somatic cells. It
is non heritable.
• Germ line gene therapy: It is the replacement of
defective gene by normal gene to germ cells. It is
to be done to avoid the inheritance of defective
gene to the next generation.
79. Methods of gene therapy
1. Viral method.
2. Non viral method.
• Viral method: in this method retrovirus are mainly
used as vector to transfer functional gene to the
target cell.
Steps involved in the viral method are,
• Selection of specific retrovirus that infect target cell.
• The virus used as vector is trimmed by removing
harmful pathogenic genes.
• The desired gene is incorporated into the vector.
• The vector is made to infect the target cells.
80. • Non viral method: Number of non viral methods
are used to transfer functional gene. Some of
them are,
1. Microinjection.
2. Electroporation.
3. Calcium phosphate mediated transfer.
• Micro injection: In this method functional gene is
directly transferred to target cell using micro
injection.
81. • Electroporation: In this method isolated cells
are subjected to low voltage electric shock. It
causes cell membrane to become permeable
for exogenous DNA.
• Calcium phosphate mediated transfer: In this
method functional gene is mixed with calcium
phosphate. This mixture is introduced near
target cells. The calcium phosphate disturbs
the cell membrane and makes permeable to
exogenous DNA
82. Embryo gene therapy through IVF-ET
( invitro fertilization and embryo transfer)
In this method gene therapy is given to the embryo through
IVF-ET. The steps involved are,
1. The gamete ovum and sperm are collected from defective
gene carrier patients.
2. The gametes are made to undergo fertilization invitro
under laboratory condition.
3. The zygote formed is incubated for three days to develop
in to 8cell stage.
4. The defective gene of the embryo cells are replaced by
normal functional gene.
5. The embryo is implanted back to the mother uterus for
further development.
6. The baby born is free from genetic disorders.
83. Monoclonal antibodies. (MABs).
• The specific antibody produced against
specific mono antigen artificially from
hybridoma cells is called monoclonal
antibodies.
• The hybridoma cells are developed by fusion
of B-lymphocytes and myeloma cells.
84.
85. Steps involved in production of monoclonal
antibodies.
• The specific mono antigen to which antibodies
are required is injected to the mouse.
• The mono antigen stimulates the immune cells
to produce specific antibody.
• The B-lymphocytes that produces the specific
antibody are isolated from the spleen of
mouse.
• The isolated B-lymphocytes and myeloma cells
( tumor cells or cancer cells) are made to
suspend in polyethylene glycol (PEG) solution.
In this media two cells fuses and develops in to
hybridoma cells.
86. • The hybridoma has the capacity to undergo
uncontrolled mitotic cell division. These cells are
allowed to undergo multiplication.
• The hybridoma cells are screened for the ability of
monoclonal antibody production.
• The hybridoma cells that produces monoclonal
antibodies are cultured in hypoxanthin aminopterin
thymidine (HAT) for production of MABs.
• Some of the cells are frozen for future use.
Note:
1. PEG – Polyethylene glycol.
2. HAT- Hypoxanthin aminopterin thymidine .
3. ELISA - Enzyme linked immune sorbent assay.
4. RIA - Radio immune assay.
87. Application of monoclonal antibodies.
1. MABs are used for identification of cancer cells,
pathogens, enzymes, hormone assay etc.
2. It is used in ELISA and RIA to measure circulating level of
hormones and enzymes.
3. The specific MAB is used for identification of HIV by ELISA
test.
4. It is used to Identify pregnancy by assaying pregnancy
hormone HCG in urine.
5. It is used to identify A, B, AB and O blood groups.
6. It is used to identify sexually transmitted diseases.
7. It is used to treat cancer.
8. It is used as immune suppresser in organ transplantation.
9. Herceptin: Genetically engineered monoclonal antibody
used to treat breast cancer.
88.
89. • U.S. govt. started Human genome project in 1986
coordinated by the Department of Energy and the
National Institutes of Health.
• GENOME – The whole hereditary information of an
organism that is encoded in the DNA is called
genome.
Aims or goal of the project:
• To identify the approximate 35,000 genes in the
human DNA.
• To determine the sequences of the 3 billion bases
that make up human DNA.
• To store this information in databases.
• To develop tools for data analysis.
• To address the ethical, legal, and social issues that
arise from genome research.
90. Achievement of HGP
• The project achieved to identify 35000 genes in
human beings.
• They sequenced about 3.2 billion base pairs in 23
pairs of chromosome
• Almost all (99.9%) nucleotide bases are exactly
the same in all people.
• The functions are unknown for over 50% of
discovered genes.
• Chromosome 1 has the most genes (2968), and
the Y chromosome has the fewest (231)
91. Application or Benefits of HGP
• It helps in understanding human genome
biology and human genetics.
• It helps to identify the gene associated with
genetic disorders.
• It helps in improving medicine and drugs.
• It helpful in giving gene therapy.
• It helps in studying human migration and
evolution.
92.
93.
94.
95.
96. Improvement of crop plants
• India planed green revolution in 1968 to over
come from the problem of scarcity of food and
starvation.
• It achieve its aim in 1978.
• Dr. swaminathan, Dr. w.K.Jain. Dr. Partha sarthi
and other agricultural scientist contributed
their work in green revolution.
• In this project crop plants are improved as
high yielding, disease resistance drought
resistance etc.
97. Indian organization for crop
production.
•
•
•
•
•
IARI – Indian Agricultural research Institute.
ICAR – Indian council of Agricultural research.
CRRI – Central Rice Research Institute.
GKVK – Gandhi Krushi vignana Kendra.
DAU – Darwad Agricultural university
98. Plant breeding technique.
1. Introduction of crop plant.
2. Selection of crop plant.- Mass selection. pure
line selection, clonal selection
3. Hybridization.
4. Polyploidy breeding.
5. Mutation breeding.
6. Tissue culture and development of transgenic
plants.
99. • Hybridization: The cross made between two
plants differing in one or more desirable
characters.
• Intra specific hybridization: It is the cross made
between two plants of different breeds.
• Inter specific hybridization: It is the cross made
between two plants of different species but
belongs to same genus.
• Inter generic hybridization: It is the cross made
between two plants of different genus belongs to
same family.
• Usually inter specific and inter generic hybrids are
sterile. Polyploidy is induced to develop them
into fertile.
100. • Polyploidy breeding: The organisms having more
than one set of chromosomes are called
polyploidy. It is induced by spraying colchicine on
seeds or seedlings.
• Mutation breeding: Any change in chromosome
or chromosome number or sequence of DNA
leads to mutation.
• Dr. Swamynathan was the first person introduced
mutation breeding in India. Hence he was
regarded as father of radiation genetics in India.
• Mutation is induced by irradiating seedlings to xrays, ϒ-rays, α-rays, β-rays. Etc.
• It is also induced by exposing seedlings to
mustered gas.
101. Tissue culture and development of
transgenic plants.
• It is the technique of culturing cells into tissue, organ or
organism on cultural media under laboratory condition.
• Totipotency: The ability of a single cell develop into a
tissue or organ or individual is called totipotency.
• The totipotency of plant cells are more than animal cells.
• Explant: Any part of the plant body or tissue that is used
in tissue culture is called explant.
• Usually parenchyma tissue of stem or root is used as
explant.
• Callus: the undifferentiated and unorganized mass of
cells developed by explant during tissue culture is called
callus.
102. Requirements for tissue culture:
• Sterilization room: this room is used to
sterilize glass equipment's and explant. It is
also used to prepare media.
• Incubation room: It is germ free room. The
room is completely sterilized by using
luminous flow bench. In this room explant is
inoculated into culture media.
• Culturing room: In this room culturing tubes
are stored. The room is maintained by proper
temp, light and optimum humidity.
103.
104. Steps in tissue culture.
• Sterilization: The sterilization of laboratory
equipment's is done by washing with potassium
dichromate solution. Further sterilization is done
by dry heat or autoclave.
• Preparation of media: The culture media is
prepared as formulated by scientist. The media
contains macro and micro nutrients. Essential
amino acids. Vitamins. Salt and some plant
hormones.
• Selection of explant: The explant is a small piece
of plant tissue. The parenchyma cells of stem,
root, apical bud meristem, or pollen grains are
used.
105. • Formation of callus: the explant selected is
sterilized and cut into number of pieces. Each
piece is inoculated into test-tube contain culture
media.
• Inoculated explant undergoes dedifferentiation
and develops in to mass of undifferentiated tissue
called callus.
• Inducing organogenesis: the organogenesis is
induced by applying different ratio of plant
hormones - Auxin and cytokinin.
• After organogenesis the seedlings having small
roots and shoot is transferred to plastic bags
containing fertile soil.
• These are grown in to small plants under green
chamber. Later plants are transferred into fields.
106. Application of tissue culture.
• Micropropogation –production of millions of
plants by tissue culture. It is applied to
increase number of crop plants, medicinal
plants, forest plants, endangered plants.
• Production of haploid plants: anther or pollen
grain culture results in development of
haploid plant. The chromosomal dabbling is
done to get diploid or polyploidy.
• Production of viral free plants: The apical bud
culture is done to develop viral free plant.
107. • Production of sec metabolic compounds: the callus
culture is transferred to bio reactor for production
of pharmaceutical compounds, alkaloids, colouring
agent etc.
• Production of transgenic plants: the desired gene is
introduced into cells of callus to develop into
transgenic plants.
108. Transgenic plants.
• The genetically modified plants developed by transferring
desired gene are called transgenic plants.
• Some transgenic plants are,
1. nif plants involve in nitrogen fixation.
2. BT plants having pest resistance.
3. Golden rice plant that fulfills vitamin A deficiency.
Importance of transgenic plants.
• Transgenic plants are developed,
1. To improve crop plants for high yield.
2. To produce disease resistance plants.
3. To produce pest resistance.
4. To produce drought resistance.
5. To produce secondary metabolite’s.
109. Golden rice:
• It is genetically modified
rice plant which is rich in
beta carotene a precursor
of vitamin A in its
endosperm.
• The golden rice was first
developed by Ingo Potrykus
(1999). The IR-64 rice is
selected to develop golden
rice.
110. • The important genes transferred
to IR-64 are,
• PSY – Phytoene synthatase.
• LYC – Lycopane cyclase. From
Daffodil plant.
• Ctrl-I gene – to synthesis
enzymes of the biosynthetic
pathway of b-carotene from
Erwinia uredovora.
111.
112. Steps in development of golden rice.
• The two specific genes PSY and LYC that involves in
production of b-carotene are isolated from daffodil plant.
• ctrl-I gene that synthesizes necessary enzyme for
production of provitamin A is isolated from the bacteria
Erwinia uredovera.
• These three genes are incorporated into Ti-plasmid to
develop r-DNA.
• This r- DNA is first transferred to bacteria Agrobacterium
tumefaciens.
• These bacteria are cultured to get number of cloned genes.
• The transformed agrobacterium is made to infect IR- 64 rice
embryo.
• the infected embryos are screened for transformed genes
and cultured.
• The seedlings produced from these cultured embryos are
called Golden rice.
113. • The seeds of golden rice are golden yellow in
colour. The density of colour depends upon
richness of b-carotene.
114. Improvement of animals.
• Animal husbandry: It is the science of raring
breeding and caring of domestic animals ( live
stock).
• Number of methods are applied to improve
animals using the knowledge of genetics and
reproductive physiology.
• The cattle breeds are mainly improved by cross
breeding.
• Cross breeding: mating of two parental animals of
different breeds to develop a hybrid is called
cross breeding.
115.
116. In cattle's it is applied to develop a hybrid in such a way
that,
1. To increase the capacity of milk production.
2. To increase lactation period up to 10 months.
3. To increase reproductive capacity.
4. To develop resistance to disease.
5. To make them to adopt tropical and sub-tropical
climates.
• Artificial insemination: it is injecting the semen of
desired bull into female reproductive tract
mechanically.
• Cryopreservation: It is preservation of semen in
liquid nitrogen at -70 C to -196 C. The frozen semen
can be stored up to 20 years.
117. Advantage of artificial insemination.
• The semen collected from single ejaculation of
bull can be inseminated to five hundred cows.
• The semen can transmitted easily than
transporting bull.
• The frozen semen can be stored upto 20 years.
Hence there is no need of maintaining large
bull yard.
118. MOET - multiple ovulation and embryo transfer
(OR) SOET - super ovulation and embryo transfer.
• Super ovulation is the
technique in which female
cow is forced to release
large number of ovum by
injecting Follicle
stimulating hormone.
• Embryo transfer is a
technique of transferring
eight cell stage embryo to
surrogate mother for
further development.
119. • Steps involved in SOET or
MOET.
• Estrus synchronization: The
donor and surrogate mother
cows are artificially made to
have same reproductive stage. It
is done by injecting gonado
tropic hormone.
• Super ovulation: The follicle
stimulating hormone is injected
to donor cow to release large
number of eggs.
120. • Artificial insemination: The
super Ovulated donor cow is
artificially inseminated by
desired bull semen.
• Embryo recovery: After
fertilization eight cell
embryos are recovered for
further development by
surgical or non-surgical
method.
121. • Embryo transfer: The collected embryos are
transferred to surrogate mother cow uterus
for further development.
• By this technique desired breed yard can be
increased in short period.
122. • IVF- ET : Invitro fertilization and embryo
transfer:
• In this technique superovulated ovum are
collected and fertilized in laboratory
condition.
• The fertilized ovum are incubated to develop
into eight cell stage.
• These embryos are transferred to surrogate
mother for further development.
123. Stem cells:
• The undifferentiated cells that have the ability
to undergo mitosis and differentiation in to
tissue are called stem cells.
• The two types of stem cells are
1. embryonic stem cells
2. Adult stem cells.
• The inner masses of cells of blastula are
embryonic stem cells.
• The bone marrow cells, placental cells are the
adult stem cells.
124. Application of stem cell culture:
• Cultured stem cells are used to differentiate into
different types of cells like liver cells, nerve cells,
muscle cells and blood cells etc.
• The differentiated tissue cells are used to treat
nervous disorders like Parkinson’s disease,
Alzheimer’s disease spinal cord injury, etc.
muscular destropy, cardiovascular disorders are
also treated.
• Cultured stem cells are used in genetic
modification.
• Stem cells are used to repair damage defective
tissue.
125. Hazards of biotechnology.
• Genetic engineering may develop new pathogen
• Genetically modified microbes may use as
biological weapons.
• Many transgenic food causes allergy in few
people.
• BT toxin transgenic plants kills and decreases the
population of different useful insects.
• Genetically modified crop plants for resistance to
weedicide can cross naturally with weeds. It
becomes difficult to control weeds.
• Wide spread of transgenic plants depletes the
agriculture biodiversity.
• Animal genes transferred to crop plants arises
ethical and religious problems.
126. Safeguard of genetic engineering.
• Strict laboratory procedure should follow in
biotechnology labs.
• Genetically engineered microbes are crippled in
such a way that they cannot survive outside the
laboratory condition.
• The permission from r-DNA advisor committee is
taken to undergo genetic work.
• The permission from genetic engineering
committee is taken to release genetically modified
organism.
• Human cloning and transgenic human experiments
should be banned.