Comparative genomics in eukaryotes, organellesKAUSHAL SAHU
WHAT IS COMPARATIVE GENOMICS?
HISTORY
SOME RELATED TERMS
MINIMAL EUKARYOTIC GENOMES
COMPARISON OF THE MAJOR SEQUENCED GENOMES
EUKARYOTIC GENOMES
SACCHAROMYCES CEREVISIAE GENOME
INSECT GENOME
DROSOPHILA MELANOGASTER (FRUIT FLY) GENOME
COMPARATIVE ANALYSIS OF THE HUMAN AND MOUSE GENOME
COMPARATIVE GENOMICS OF ORGANELLES
COMPARATIVE GENOMICS TOOLS
CONCLUSION
REFERENCES
Nuclear Genomes(Short Answers and questions)Zohaib HUSSAIN
1. What did researchers find when they sequenced the centromeres of Arabidopsis? Why was this finding surprising?
Ans: Before the Arabidopsis sequences were obtained it was thought that these repeat sequences were by far the principal component of centromeric DNA. However, Arabidopsis centromeres also contain multiple copies of genome-wide repeats, along with a few genes, the latter at a density of 7–9 per 100 kb compared with 25 genes per 100 kb for the noncentromeric regions of Arabidopsis chromosomes. The discovery that centromeric DNA contains genes was a big surprise because it was thought that these regions were genetically inactive.
2. What differences in gene distribution and repetitive DNA content are seen when yeast and human chromosomes are compared?
Ans. A typical region of a human chromosome will have few genes (most of which will contain introns), several repeated sequences, and a large amount of nonrepetitive, nongenic DNA. Yeast chromosomes have higher gene densities, with very few genes containing introns, and have few repeated sequences and much less nongenic DNA.
3. The human genome contains about 50,000 fewer genes than was predicted by many researchers. Why were these initial predictions so high?
Ans. These early estimates were high because they were based on the supposition that, in most cases, a single gene specifies a single mRNA and a single protein. According to this model, the number of genes in the human genome should be similar to the number of proteins in human cells, leading to the estimates of 80,000–100,000. The discovery that the number of genes is much lower than this indicates that alternative splicing, the process by which exons from a pre-mRNA are assembled in different combinations so that more than one protein can be coded by a single gene is more prevalent than was originally appreciated.
4. What are the different methods used to catalog genes? What are the advantages or disadvantages of these methods?
Ans. Gene catalogs can be based on the known functions of genes, but such catalogs are incomplete because in most genomes many genes have unknown functions. Gene catalogs that are based on the identities of protein domains coded by genes are more comprehensive as these include many genes whose specific functions are unknown.
5. What is the function of the different genes in the human globin gene families?
Ans. The globins are the blood proteins that combine to make hemoglobin, each molecule of hemoglobin being made up of two a-type and two b-type globins.The a-globin cluster is located on chromosome 16 and the b-cluster on chromosome 11. Both clusters contain genes that are expressed at different developmental stages and each includes at least one pseudogene. Note that expression of the a-type gene x2 begins in the embryo and continues during the fetal stage; there is no fetal-specific a-type globin. The q pseudogene is expressed but its protein product is inactive. None of the other p
Guest lecture on comparative genomics for University of Dundee BS32010, delivered 21/3/2016
Workshop/other materials available at DOI:10.5281/zenodo.49447
This is the first presentation of the BITS training on 'Comparative genomics'.
It reviews the basic concepts of sequence homology on different levels.
Thanks to Klaas Vandepoele of the PSB department.
Slides from a Comparative Genomics and Visualisation course (part 1) presented at the University of Dundee, 7th March 2014. Other materials are available at GitHub (https://github.com/widdowquinn/Teaching)
Comparative genomics in eukaryotes, organellesKAUSHAL SAHU
WHAT IS COMPARATIVE GENOMICS?
HISTORY
SOME RELATED TERMS
MINIMAL EUKARYOTIC GENOMES
COMPARISON OF THE MAJOR SEQUENCED GENOMES
EUKARYOTIC GENOMES
SACCHAROMYCES CEREVISIAE GENOME
INSECT GENOME
DROSOPHILA MELANOGASTER (FRUIT FLY) GENOME
COMPARATIVE ANALYSIS OF THE HUMAN AND MOUSE GENOME
COMPARATIVE GENOMICS OF ORGANELLES
COMPARATIVE GENOMICS TOOLS
CONCLUSION
REFERENCES
Nuclear Genomes(Short Answers and questions)Zohaib HUSSAIN
1. What did researchers find when they sequenced the centromeres of Arabidopsis? Why was this finding surprising?
Ans: Before the Arabidopsis sequences were obtained it was thought that these repeat sequences were by far the principal component of centromeric DNA. However, Arabidopsis centromeres also contain multiple copies of genome-wide repeats, along with a few genes, the latter at a density of 7–9 per 100 kb compared with 25 genes per 100 kb for the noncentromeric regions of Arabidopsis chromosomes. The discovery that centromeric DNA contains genes was a big surprise because it was thought that these regions were genetically inactive.
2. What differences in gene distribution and repetitive DNA content are seen when yeast and human chromosomes are compared?
Ans. A typical region of a human chromosome will have few genes (most of which will contain introns), several repeated sequences, and a large amount of nonrepetitive, nongenic DNA. Yeast chromosomes have higher gene densities, with very few genes containing introns, and have few repeated sequences and much less nongenic DNA.
3. The human genome contains about 50,000 fewer genes than was predicted by many researchers. Why were these initial predictions so high?
Ans. These early estimates were high because they were based on the supposition that, in most cases, a single gene specifies a single mRNA and a single protein. According to this model, the number of genes in the human genome should be similar to the number of proteins in human cells, leading to the estimates of 80,000–100,000. The discovery that the number of genes is much lower than this indicates that alternative splicing, the process by which exons from a pre-mRNA are assembled in different combinations so that more than one protein can be coded by a single gene is more prevalent than was originally appreciated.
4. What are the different methods used to catalog genes? What are the advantages or disadvantages of these methods?
Ans. Gene catalogs can be based on the known functions of genes, but such catalogs are incomplete because in most genomes many genes have unknown functions. Gene catalogs that are based on the identities of protein domains coded by genes are more comprehensive as these include many genes whose specific functions are unknown.
5. What is the function of the different genes in the human globin gene families?
Ans. The globins are the blood proteins that combine to make hemoglobin, each molecule of hemoglobin being made up of two a-type and two b-type globins.The a-globin cluster is located on chromosome 16 and the b-cluster on chromosome 11. Both clusters contain genes that are expressed at different developmental stages and each includes at least one pseudogene. Note that expression of the a-type gene x2 begins in the embryo and continues during the fetal stage; there is no fetal-specific a-type globin. The q pseudogene is expressed but its protein product is inactive. None of the other p
Guest lecture on comparative genomics for University of Dundee BS32010, delivered 21/3/2016
Workshop/other materials available at DOI:10.5281/zenodo.49447
This is the first presentation of the BITS training on 'Comparative genomics'.
It reviews the basic concepts of sequence homology on different levels.
Thanks to Klaas Vandepoele of the PSB department.
Slides from a Comparative Genomics and Visualisation course (part 1) presented at the University of Dundee, 7th March 2014. Other materials are available at GitHub (https://github.com/widdowquinn/Teaching)
Pattern making , Dressmaking work are done by Asmaa Salah Eldin for more information follow the link:
https://www.linkedin.com/profile/view?id=AAIAABQB8ecB8Sa6arAPxwVWc45ln01FfwEu0_Y&trk=nav_responsive_tab_profile
SOCIAL MEDIA MARKETING - CONNECTING THE WORLDUsha Ramkumar
Grow your Business by connecting with the right audience through social media channels and build a long-term relationship
Brand Visibility
Social media marketing refers to the process of gaining traffic to your website and brand visibility through social media sites.
Increased Conversions
Social media marketing programs center on efforts to create content that attracts attention and encourages readers to share it across their social networks and hence increase sales.
eVenueBooking, the largest online resource for banquet halls, party venues, event venues in Philadelphia, USA – shares some quick bytes for you to get there!
this is done by me and my team mates of Wayamba University Sri Lanka for our project.From now we decided to allow download this file.I would be greatful if you could send your comments..
And I'm willing to help you in similar works.I'm in final year of my degree(.BSc Biotechnology)..
pubudu_gokarella@yahoo.com
The project was a great success, delivering the first rough draft human genome sequence in 2000 and the final high-quality version in April, 2003, ahead of schedule and under budget. For years, many considered the Human Genome Project to be biology's equivalent to "Man on the moon". This slide tends to explain the benefits of such project to medical diagnosis, treatment and management in India.
The Human Genome Project (HGP) was an international scientific research project with the goal of determining the base pairs that make up human DNA, and of identifying and mapping all of the genes of the human genome from both a physical and a functional standpoint.
What is bioinformatics?
About human genome
Human genome project
Aim of human genome project
History
Sequencing Strategy
Benefits of Human Genome Project research
Disadvantages of human genome project
Conclusion
References
Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble and analyze the function and structure of genomes
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
HOT NEW PRODUCT! BIG SALES FAST SHIPPING NOW FROM CHINA!! EU KU DB BK substit...GL Anaacs
Contact us if you are interested:
Email / Skype : kefaya1771@gmail.com
Threema: PXHY5PDH
New BATCH Ku !!! MUCH IN DEMAND FAST SALE EVERY BATCH HAPPY GOOD EFFECT BIG BATCH !
Contact me on Threema or skype to start big business!!
Hot-sale products:
NEW HOT EUTYLONE WHITE CRYSTAL!!
5cl-adba precursor (semi finished )
5cl-adba raw materials
ADBB precursor (semi finished )
ADBB raw materials
APVP powder
5fadb/4f-adb
Jwh018 / Jwh210
Eutylone crystal
Protonitazene (hydrochloride) CAS: 119276-01-6
Flubrotizolam CAS: 57801-95-3
Metonitazene CAS: 14680-51-4
Payment terms: Western Union,MoneyGram,Bitcoin or USDT.
Deliver Time: Usually 7-15days
Shipping method: FedEx, TNT, DHL,UPS etc.Our deliveries are 100% safe, fast, reliable and discreet.
Samples will be sent for your evaluation!If you are interested in, please contact me, let's talk details.
We specializes in exporting high quality Research chemical, medical intermediate, Pharmaceutical chemicals and so on. Products are exported to USA, Canada, France, Korea, Japan,Russia, Southeast Asia and other countries.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
Follow us on: Pinterest
Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
1. BIOINFORMATICS FOR BETTER TOMORROW
B. Jayaram, N. Latha, Pooja Narang, Pankaj Sharma, Surojit Bose, Tarun Jain, Kumkum
Bhushan, Saher Afshan Shaikh, Poonam Singhal, Gandhimathi and Vidhu Pandey
Department of Chemistry &
Supercomputing Facility for Bioinformatics & Computational Biology,
Indian Institute of Technology, Hauz Khas, New Delhi - 110016, India.
Email: bjayaram@chemistry.iitd.ac.in
Web site: www.scfbio-iitd.org
I. What is Bioinformatics?
Bioinformatics is an emerging interdisciplinary area of Science & Technology
encompassing a systematic development and application of IT solutions to handle
biological information by addressing biological data collection and warehousing, data
mining, database searches, analyses and interpretation, modeling and product design.
Being an interface between modern biology and informatics it involves discovery,
development and implementation of computational algorithms and software tools that
facilitate an understanding of the biological processes with the goal to serve primarily
agriculture and healthcare sectors with several spin-offs. In a developing country like
India, bioinformatics has a key role to play in areas like agriculture where it can be used
for increasing the nutritional content, increasing the volume of the agricultural produce
and implanting disease resistance etc.. In the pharmaceutical sector, it can be used to
reduce the time and cost involved in drug discovery process particularly for third world
diseases, to custom design drugs and to develop personalized medicine (Fig. 1).
Gene finding Protein structure prediction Drug design
Figure 1 : Some major areas of research in bioinformatics and computational biology.
2. Figure 2 : The tree of life depicting evolutionary relationships among organisms from the
major biological kingdoms. A possible evolutionary path from a common ancestral cell to
the diverse species present in the modern world can be deduced from DNA sequence
analysis. The branches of the evolutionary tree show paths of descent. The length of paths
does not indicate the passage of time and the vertical axis shows only major categories of
organisms, not evolutionary age. Dotted lines indicate the supposed incorporation of
some cell types into others, transferring all of their genes and giving the tree some web-
like features. (Source : A Alberts, D Bray, J Lewis, M Raff, K Roberts & J D Watson, Molecular
Biology of the Cell, p38, Garland, New York (1994)).
3. It is assumed that life originated from a common ancestor and all the higher
organisms evolved form a common unicellular prokaryotic organism. Subsequent
division of different forms of life from this makes the diversity in the morphological and
genetic characters (Fig. 2).
Figure 3 : (A) An animal cell. The figure represents a rat liver cell, a typical higher
animal cell in which features of animal cells are evident such as nucleus, nucleolus,
mitochondria, Golgi bodies, lysosomes and endoplasmic reticulum (ER). (Source:
www.probes.com/handbook/ figures/0908.html) (B). A plant cell (cell in the leaf of a higher
plant). Plant cells in addition to plasma membrane have another layer called cell wall,
which is made up of cellulose and other polymers where as animal cells have plasma
membrane only. The cell wall, membrane, nucleus chloroplasts, mitochondria, vacuole,
ER and other organelles that make up a plant cell are featured in the figure (Source:
http://www.sparknotes.com/biology/cellstructure/celldifferences/section1.html).
(A). Animal cell (B). Plant cell
The common basis to all these diverse organisms is the basic unit known as the
cell (Fig. 3). All cells whether they belong to a simple unicellular organism or a complex
multicellular organism (human adults comprise ~ 30 trillion cells), possess a nucleus
which carries the genetic material consisting of polymeric chains of DNA (deoxyribo
nucleic acid), holding the hereditary information and controlling the functioning. Several
challenges lie ahead in deciphering how DNA, the genetic material in these cells
eventually leads to the formation of organisms (Fig. 4).
4. CELL
TISSUE
ORGAN
ORGANISM
Figure 4 : Levels of organization. The entire DNA content in a cell is called the genome.
In spite of the complex organization, cells of all organisms possess different
The entire protein content in a cell is called the proteome. Cellome is the entire
complement of molecules, including genome and proteome within a cell. Tissues are
made of collections of cells. Tissue collections make organs. An organism is a collection
of several organ systems.
molecules of life for the maintenance of living state. Theses molecules include nucleic
acids, proteins, carbohydrates and lipids (Fig. 5).
5. Nucleic Acids
Carbohydrates
Proteins
Lipids
Figure 5 : Biom
All organisms self re genetic material DNA, the
olecules of life
plicate due to the presence of
polynucleotide consisting of four bases Adenine (A), Thymine (T), Guanine (G) and
Cytosine (C) (Fig. 6). The entire DNA content of the cell is what is known as the genome.
The segment of genome that is transcribed into RNA is called gene. So we can say that
hereditary information is transferred in the form of genes contained on the four bases.
Understanding these genes is one of the modern day challenges. Why only five percent of
the entire DNA is in the form of genes and what is the rest of the DNA responsible for,
under what conditions genes are expressed, where, when and how to regulate gene
expression are some unsolved puzzles.
6. Figure 6 : DNA and its alphabets A, G, C & T the nucleic acid bases. (Source: Molecular
rmation present in DNA is expressed via RNA molecules into proteins
biology of the cell, Garland Publishing. Inc. New York, 1994 )
The info
which are responsible for carrying out various activities. This information flow is called
the central dogma of molecular biology (Fig. 7). Potential drugs can bind to DNA, RNA
or proteins to suppress or enhance the action at any stage in the pathway.
7. Figure 7 : Central dogma of modern biology
enome Analysis
f genome called genes determine the sequence of amino acids in
otein
n order to identify all
average of just 2% i.e. just about 160 enzymes.
G
Segments o
pr s. The mechanism is simple for the prokaryotic cell where all the genes are
converted into the corresponding mRNA (messenger ribonucleic acid) and then into
proteins. The process is more complex for eukaryotic cells where rather than full DNA
sequence, some parts of genes called exons are expressed in the form of mRNA
interrupted at places by random DNA sequences called introns. Of the several questions
posed here, one is that how some parts of the genome are expressed as proteins and yet
other parts (introns as well as intergenic regions) are not expressed.
Scores of genome projects are being carried out world wide i
the genes and ascertain their functions in a specified organism. Human genome project
is one such global effort to identify all the alphabets on the human genome, initiated in
1990 by the US government. A comparison of the genome sizes of different organisms
(Table 1) raises questions like what types of genetic modifications are responsible for the
four times large genome size of wheat plant and seven times small size of the rice plant
as compared to that of humans. Mice and humans contain roughly the same number of
genes – about 28K protein coding regions. The chimp and human genomes vary by an
RNA
Genome
Gene = DNA
DNA binding drugs Protein
Gene therapy Primary Sequence
RPRTAFSSEQLARLK
REFNENRYLTERRR
QQLSSELGLNEAQIK
IWFQNKRAKIKKS
RNA binding drugs Drugs
Inhibitors/activators
8. Table 1 : Genome sizes of some organisms
Organism Genome size (Mb)
(Mb=Mega base)
Eschericia coli 4.64
M t suberculosi 4.4
H.Influenza 1.83
Homo sapiens (humans) 3300
Mouse 3000
Rice 430
Wheat 13500
(source : http://users m/jkimball.ma.ultranet/BiologyPages nomeSizes.html)
Table 2 : Specific genetic disorders
.rcn.co /G/Ge
Genetic Disorder Reason
Huntington’s Disease Excessive repeats of a three-base sequence, "CAG" on
chromosome 4.
Parkinson’s Disease Variations in genes on chromosomes 4,6.
Sickle Cell Disease Mutation in hemoglobin-b gene on chromosome 11
Tay-Sachs Disease Co 15ntrolled by a pair of genes on chromosome
Cystic Fibrosis Mutations in a single (CFTR) gene
Breast Cancer Mutation on genes found on chromosomes 13 & 17
Leukemia Exchange arms ofof genetic material between the long
chromosome 6 & 22.
Colon cancer Proteins MSH2, MSH6 on chromosome 2 & MLH1 on
chromosome 3 are mutated.
Asthma Disfunctioning of genes on chromosome 5,6,11,14&12.
Rett Syndrome Disfunctioning of a gene on the X chromosome.
Bruk omaitt lymph Translocations on chromosome 8
Alzheimer disease Mutat 9 &ions on four genes located on chromosome 1, 14, 1
21.
Werner Syndrome Mutat me 8.ions on genes located on chromoso
Angelman Syndrome Deletion of a segment on maternally derived chromosome 15.
Best disease Mutat e 11.ion in one copy of a gene located on chromosom
Pendred Syndrome Defective gene on chromosome 7.
Dias siatrophic dyspla Mutation in a gene on chromosome 5
(Source:ht h.gov/)tp://www.ncbi.nlm.ni
9. Several genetic disorders like Huntington’s disease, Parkinson’s disease, sickle
cell anemia etc. are caused due to mutations in the genes or a set of genes inherited from
one generation to another (Table 2). There is a need to understand the cause for such
disorders. Why nature carries such disorders and how to prevent these are some of the
areas where extensive research endeavor has to be invested.
An understanding of the genome organization can lead to concomitant progresses
in drug-target identification. Comparative genomics has become a very important
emerging branch with tremendous scope, for the above mentioned reasons. If the genome
for humans and a pathogen, a virus causing harm is identified, comparative genomics can
predict possible drug-targets for the invader without causing side effects to humans (Fig.
8).
Potential Areas for Drug Targets = Hc
∩ I
H = Human Genome / Proteome (Healthy Individual)
I = Genome / Proteome of the Invader / Pathogen
Figure 8 : Comparative genomics for drug target identification – an application of
Bioinformatics
Over the past two decades genetic modification has enabled plant breeders to
develop new varieties of crops like cereals, soya, maize at a faster rate. Some of these
called as transgenic varieties have been engineered to possess special characteristics that
make them better. Recently efforts are on in the area of utilizing GM (genetically
modified) crops to produce therapeutic plants.
Another area where bioinformatics techniques can play an important role is in
SNP (Single nucleotide polymorphisms) discovery and analysis. SNPs are common DNA
sequence variations that occur when a single nucleotide in the genome sequence is
changed. SNP’s occur every 100 to 300 bases along the human genome. The SNP
10. variants promise to significantly advance our ability to understand and treat human
diseases.
Given the whole genome of an organism, finding the genes (gene annotation) is a
challenging task. Various approaches have been utilized by different groups for this.
These approaches are typically database driven which rely on the already known
forma
r which attempts to capture forces responsible for DNA structure and protein-DNA
interactions walks along the length of the genome distinguishing genes from non-genes.
As of now, the physico-chemical model (ChemGene 1.0) is able to distinguish
successfully genes from non-genes in 120 prokaryotic genomes with fairly good
specificities and sensitivities.
Protein Folding
Proteins are polymers of amino acids with unlimited potential variety of structures
and metabolic activiti e dimensional shape
called its confo its shape and
genetic code. Substitution of a single amino acid can cause a major
tist made some significant contributions
energy which is about 10 to 15 kcal/mol relative to the unfolded state. How does a given
sequence of amino acids fold into a specific conformation as soon as it is conceived on
in tion. The Markov model based methods utilize short range correlations between
bases along the genome. Other methods based on Fourier transform techniques
emphasize global correlations.
At IIT Delhi, we are attempting to develop a hypothesis driven physico-chemical
model for genome analysis and for distinguishing genes from non-genes. In this model, a
vecto
es. Each protein possesses a characteristic thre
rmation. Sequence of amino acids of protein determines
function. This in turn is genetically controlled by the sequences of bases in DNA of the
cell through the
alteration in function. Study of homologous proteins from different species is of interest
in constructing taxonomic relationships. Proteins may be classified into structural
proteins, enzymes, hormones, transport proteins, protective proteins, contractile proteins,
storage proteins, toxins etc..
Prof G N Ramachandran, an Indian scien
towards an understanding of the secondary structure of proteins. His fundamental work in
this area is remembered in the form of Ramachandran maps.
Protein folding can be considered to involve changes in the polypeptide chain
conformation to attain a stable conformation corresponding to the global minimum in free
11. the ribosomal machinery using the information on mRNA in millisecond timescales, is
the problem pending a resolution for over five decades. For a 200 amino acid protein with
just two conformations per amino acid, a systematic search for this minimum among all
possible 2200
conformations, will take approximately 3x1054
years which is much longer
than the present age of the universe. Despite this innumerable number of conformations
to search, nature does it in milliseconds to seconds (Fig. 9).
Figure 9 : The protein folding problem (Source: press2.nci.nih.gov/.../ snps_cancer21.htm)
Solution to the protein folding problem has an immense immediate impact on
society. In biotech industry, this can be helpful in the design of nanobiomachines and
sed drug discovery wherein
the stru
biocatalysts to carry out the required function. Pulp, paper and textile industry, food and
beverages industry, leather and detergent industry are among the several potential
beneficiaries. Other important implications are in structure ba
cture of the drug target is vital (Fig. 10). Structures of receptors – a major of class
of drug targets - are refractory to experimental techniques thus leaving the fold open to
computer modeling.
12. Figure 10 : Drug targets (Source: Drews J., Drug Discovery: A historical perspective, Science, 2000,
287: 1960-1964)
There is an urgent demand for faster and better algorithms for protein structure
prediction. There are three major ways in which protein structure prediction attempts are
currently progressing viz. homology modeling, fold recognition and ab initio approaches.
Whereas the first two approaches are database dependent relying on known structures and
folds of proteins, the third one is independent of the databases and starts from the
physical principles. Although the ab initio techniques till date are able to predict only
small proteins, because of their first principle approach, they have the potential to predict
new / novel folds and structures. On account of the complexity of the problem,
computational requirement for such a prediction is a major issue which can easily be
appreciated by the estimates of time required to fold a 200 amino acid protein which
evolves ~10-11
sec per day per processor according to Newton’s laws of motion. This will
require approximately a million years to fold a single protein. If one can envision a
million proc tein can be folded in one ear computer
e. In this Blue Gene
essors working together, a single pro y
tim spirit, the IBM has launched a five year project in the year 1999
to address the complex biomolecular phenomena such as protein folding.
At IIT Delhi, we are developing a computational protocol for modeling and
predicting protein structures of small globular proteins. Here a combination of
bioinformatics tools, physicochemical properties of proteins and ab initio approaches are
used. Starting with the sequence of amino acids, for ten small alpha helical proteins,
structures to within 3-5Å of the native are predicted within a day on a 50 ultra sparc III
Cu 900 MHz processor cluster. Attempts are on to further bring the structures to within
13. <3Å of the native structures via molecular dynamics and Metropolis Monte Carlo
simulations.
Drug Design
As structures of more and more protein targets become available through
crystallography, NMR and bioinformatics methods, there is an increasing demand for
computational tools that can identify and analyze active sites and suggest potential drug
molecules that can bind to these sites specifically (Fig. 11).
Figure 11 : Active-site directed drug-design
Also to combat life-threatening diseases such as AIDS, Tuberculosis, Malaria etc.,
a global push is essential (Table 3). Millions for Viagra and pennies for the diseases of
the poor is the current situation of investment in Pharma R&D.
Active Site
14. Table 3 : WHO Calls for Global Push Against AIDS & Tuberculosis & Malaria
(Source : www.globalhealth.org, www.thenation.com )
Time and cost required for designing a new drug are immense and at an
unacceptable level. According to some estimates it costs about $880 million and 14 years
of research to develop a new drug before it is introduced in the m
Lead Generation & Lead Optimization
3.0 yrs 15%
Preclinical Development
1.0 yrs 10%
Phase I, II & III Clinical Trials
6.0 yrs 68%
FDA Review & Approval
1.5 yrs 3%
Drug to the Market
14 yrs $880million
arket (Table 4).
Table 4 : Cost and time involved in drug discovery
Target Discovery
2.5 yrs 4%
(Source : PAREXEL, PAREXEL’s Pharmaceutical R&D Stastical Sourcebook, 2001, p96)
illion die each year due to diseases like diarrhoea, small pox, polio,
river blindness, leprosy.
cost $ 12 billion to fight the disease of poverty
(AIDS medication about $15K per annum)
Millions for Viagra, Pennies for the Diseases of the Poor
Of all new medications brought to the market (1223) by Multinationals from
1975 only 1% (13) are for tropical diseases plaguing the third world.
Life style drugs dominate Pharma R&D
(1) Toe nail Fungus (2) Obesity
(3) Baldness (4) Face Wrinkle
(5) Erectile Dysfunction (6) Separation anxiety of dogs etc.
Nearly 6 m
Estimated
A new economic analysis
Healthy people can get themselves out of poverty.
Relieving a population of burden of the diseases for 15 to 20 years will give a
huge boost to economic development.
Nearly 6 million die each year due to diseases like diarrhoea, small pox, polio,
river blindness, leprosy.
cost $ 12 billion to fight the disease of poverty
(AIDS medication about $15K per annum)
Millions for Viagra, Pennies for the Diseases of the Poor
Of all new medications brought to the market (1223) by Multinationals from
1975 only 1% (13) are for tropical diseases plaguing the third world.
Life style drugs dominate Pharma R&D
(1) Toe nail Fungus (2) Obesity
(3) Baldness (4) Face Wrinkle
(5) Erectile Dysfunction (6) Separation anxiety of dogs etc.
Estimated
A new economic analysis
Healthy people can get themselves out of poverty.
Relieving a population of burden of the diseases for 15 to 20 years will give a
huge boost to economic development.
15. Intervention of computers at some plausible steps is imperative to bring down the cost
an
Fig re 12
E
for a given drug
d time required in the drug discovery process (Fig. 12, Table 5).
u : Potential areas for In silico intervention in drug-discovery process
Table 5 : High End Computing Needs for In Silico Drug Design
stimates of current computational requirements to complete a binding affinity calculation
(Source : http://cbcg.lbl.gov)
In silico methods can help in identifying drug targets via bioinformatics tools.
They can also b g / active sites,
generate candidate m
Modeling complexity Method Size of Required computing
library time
Molecular Mechanics SPECITOPE 140,000 ~1 hour
Rigid ligand/target LUDI 30,000 1-4 hours
CLIX 30,000 33 hours
Molecular Mechanics Hammerhead 80,000 3-4 days
Partially flexible ligand DOCK 17,000 3-4 days
Rigid target DOCK 53,000 14 days
e used to analyze the target structures for possible bindin
olecules, check for their drug-likeness, dock these molecules with
Molecular Mechanics
Fully flexible ligand
Rigid target
ICM 100,000 ~1 year
(extrapolated)
Molecular Mechanics
Free energy
perturbation
AMBER
CHARMM
1 ~several days
QM Active site and
MM protein
Gaussian,
Q-Chem
1 >several weeks
16. the target, rank them according to their binding affinities, further optimize the molecules
to improve binding characteristics etc..
-50
0
-30
-20
-10
0
10
20
30
BindingFreeEnergy(kcal/mol)
Pursuing the dream that once the gene target is identified and validated drug
discovery protocols could be automated using Bioinformatics & Computational Biology
tools, at IIT Delhi we have developed a computational protocol for active site directed
drug design. The suite of programs (christened “Sanjeevini”) has the potential to evaluate
and /or generate lead-like molecules for any biological target. The various modules of
this suite are designed to ensure reliability and generality. The software is currently being
optimized on Linux and Sun clusters for faster and better results. Making a drug is more
like designing an adaptable key for a dynamic lock. The Sanjeevini methodology consists
of design of a library of templates, generation of candidate inhibitors, screening
candidates via drug-like filters, parameter derivation via quantum mechanical
calculations for energy evaluations, Monte Carlo docking and binding affinity estimates
e protocols
tested on Cyclooxygenase-2 as a target could successfully distinguish NSAIDs
(Nonsteroid on other
(red).
based on post facto analyses of all atom molecular dynamics trajectories. Th
al Anti-inflammatory drugs) from non-drugs (Fig 13). Validation
targets is in progress.
-4
Figure 13 : The active site directed lead design protocols developed from first principles
and implemented on a supercomputer could segregate NSAIDS (blue) from Non-drugs
17. Bioinformatics and Biodiversity
Biodiversity informatics harnesses the power of computational and information
technologies to organize and analyze data on plants and animals at the macro and at
genome levels. India ranks among the top twelve nations of the world in terms of
biological diversity (Fig. 14 and Fig15).
Himalayas - This majestic range of
mountains is the home of a diverse range
of flora and fauna. Eastern Himalayas is one
iversity hotspots in India.
Chilika - This wetland area is protected
under the Ramsar convention.
of the two biod
Sunderbans - The largest mangrove forest in Western Ghats - One of the two
India. biodiversity hotspots in India.
Thar desert ntrast to the Himalayan region.
(Source: http://edugreen.teri.res.in/explore/maps/biodivin.htm)
- The climate and vegetation in this area is a co
Figure 14 : Biodiversity in India
18. Figure 15: Biodiversity bioinformatics is essential to preserve the natural balance of flora
and fauna on the planet and to prevent extinction of species (Source: www.hku.hk/
ecology/envsci.htm).
Bioinformatics endeavors in India
Owing to the well acknowledged IT skills and a spate of upcoming software,
biotech and pharma industries and active support from Government organizations, the
field of Bioinformatics appears promising. The projections of the growth potential in
India in a global scenario however belie these expectations (Fig. 16).
Figure 16 : Growth potential for Bioinformatics based business opportunities in India
ccording to IDC (International Data Corporation, India.)a
0
0.2
0.8
1
1.2
1.4
1.6
1.8
1
0.4
0.6
2
Year2001 2005
Global
India
19. Creating the necessary infrastructure around specialists to meet the high
omputational power required for these activities (Table 6, Fig. 17) is one step in the
ght direction.
able 6 : Geno ate of computational
quirements
. Gene Prediction
c
ri
T me to drug discovery research: A rough estim
re
1
Homology/string comparison. 300 Giga flop
~ 3*109
bp
2. Protein Structure Prediction
- Threading 100 Giga flop
- Statistical Models
- Filters to reduce plausible structures
Molecular Dynamics
s per simulation 25000
Active site directed drug design
100 structures 30 Peta flop
1-ns simulation for structure refinement
Total Compute Time 5000ns
Number of atom
3.
Scan 1000 drug molecules/protein 18 Peta flop
Total Computational requirement to design one lead compound from genome
16
o design ten lead compounds per day (on a dedicated machine)
e requirement is 5.8 tera flops capacity.
ut of every 100 lead compounds, only one may become a drug, which further increases
e computer requirements)
3ns simulation per drug molecule
(Active site searches, docking, rate and affinity determinations etc.)
Total Compute Time 3000ns
25000 atoms per simulation
Summary
~ 50 Peta flop (5.x10 floating point operations)
T
th
(O
th
20. for bioinformatics
and scientific community from across the country,
l Private
iotechnology, Govt of India). It is envisioned that the
span all the 60+ Bioinformatics Centres of the Department of Biotechnology
e 18).
Figure 17 : Challenges
To enable access by the student
the Supercomputing Facility at IIT Delhi is connected to Biogrid-India (a Virtua
Network of the Department of B
Biogrid will
with GBPS bandwidth and at least 10 teraflops of compute capacity soon (Figur
Transcription
Model
Education RNA splicing
model
Signal
transduction
pathways
Protein:DNA A/RN
protein:protein
Protein structure
prediction
Drug Design
Protein
Evolution
Gene ontologies
Speciation
Challenges for
Bioinformatics
21. Figure 18 : Biogrid-India
Pro-active policies and conducive environment to researchers, entrepreneurs and
Industry will go a long way in steering India towards leadership in the area of
Bioinformatics and Computational Biology.
Acknowledgements.
BJ is thankful to the Department of Biotechnology, the Department of Science &
Technology, Council of Scientific & Industrial Research, Indo-French Centre for the
Promotion of Advanced Research and IIT Delhi administration for support over the years.