Comparative genomics involves analyzing and comparing the genomes of different organisms to understand evolution, gene function, and disease. Genomes can be compared at multiple levels, including overall structure, coding regions, and non-coding regions. Comparing gene location, structure, characteristics, and sequence similarity provides insights into how genomes have changed over time and what distinguishes species. Comparative genomics also aids in drug discovery by identifying potential drug targets and furthering our understanding of pathogenesis. The field has wide-ranging impacts on biology, agriculture, medicine, and conservation efforts.
Comparative genomics in eukaryotes, organellesKAUSHAL SAHU
WHAT IS COMPARATIVE GENOMICS?
HISTORY
SOME RELATED TERMS
MINIMAL EUKARYOTIC GENOMES
COMPARISON OF THE MAJOR SEQUENCED GENOMES
EUKARYOTIC GENOMES
SACCHAROMYCES CEREVISIAE GENOME
INSECT GENOME
DROSOPHILA MELANOGASTER (FRUIT FLY) GENOME
COMPARATIVE ANALYSIS OF THE HUMAN AND MOUSE GENOME
COMPARATIVE GENOMICS OF ORGANELLES
COMPARATIVE GENOMICS TOOLS
CONCLUSION
REFERENCES
description of functional genomics and structural genomics and the techniques involved in it and also decribing the models of forward genetics and techniques involved in it and reverse genetics and techniques involved in it
Transcriptomics is the study of RNA, single-stranded nucleic acid, which was not separated from the DNA world until the central dogma was formulated by Francis Crick in 1958, i.e., the idea that genetic information is transcribed from DNA to RNA and then translated from RNA into protein.
Comparative genomics in eukaryotes, organellesKAUSHAL SAHU
WHAT IS COMPARATIVE GENOMICS?
HISTORY
SOME RELATED TERMS
MINIMAL EUKARYOTIC GENOMES
COMPARISON OF THE MAJOR SEQUENCED GENOMES
EUKARYOTIC GENOMES
SACCHAROMYCES CEREVISIAE GENOME
INSECT GENOME
DROSOPHILA MELANOGASTER (FRUIT FLY) GENOME
COMPARATIVE ANALYSIS OF THE HUMAN AND MOUSE GENOME
COMPARATIVE GENOMICS OF ORGANELLES
COMPARATIVE GENOMICS TOOLS
CONCLUSION
REFERENCES
description of functional genomics and structural genomics and the techniques involved in it and also decribing the models of forward genetics and techniques involved in it and reverse genetics and techniques involved in it
Transcriptomics is the study of RNA, single-stranded nucleic acid, which was not separated from the DNA world until the central dogma was formulated by Francis Crick in 1958, i.e., the idea that genetic information is transcribed from DNA to RNA and then translated from RNA into protein.
STS stands for sequence tagged site which is short DNA sequence, generally between 100 and 500 bp in length, that is easily recognizable and occurs only once in the chromosome or genome being studied.
Introduction
Transcriptome analysis
Goal of functional genomics
Why we need functional genomics
Technique
1. At DNA level
2.At RNA level
3. At protein level
4. loss of function
5. functional genomic and bioinformatics
Application
Latest research and reviews
Websites of functional genomics
Conclusions
Reference
What is Genome,Genome mapping,types of Genome mapping,linkage or genetic mapping,Physical mapping,Somatic cell hybridization
Radiation hybridization ,Fish( =fluorescence in - situ hybridization),Types of probes for FISH,applications,Molecular markers,Rflp(= Restriction fragment length polymorphism),RFLPs may have the following Applications;Advantages of rflp,disAdvantages of rflp, Rapd(=Random amplification of polymorphic DNA),Process of rapd, Difference between rflp &rapd
Genomics and its application in crop improvementKhemlata20
meaning ,definition of genome ,genomics ,tools of genomics ,what is genome sequencing ,methods of genome sequencingand genome mapping ,advantage of genomics over traditional breeding program, examples of some crops whose genome has been sequenced, important points about genomics, work in the field of genomics ,applications of genomics .classification of genomics .different Omics in genomics like Proteomics ,Transcriptomics ,Metabolomics ,Need of genome sequencing
A physical map of a chromosome or a genome that shows the physical locations of genes and other DNA sequences of interest. Physical maps are used to help scientists identify and isolate genes by positional cloning.
According to the ICSM (Intergovernmental Committee on Surveying and Mapping), there are five different types of maps: General Reference, Topographical, Thematic, Navigation Charts and Cadastral Maps and Plans.
DNA SEQUENCING METHODS AND STRATEGIES FOR GENOME SEQUENCINGPuneet Kulyana
This presentation will give you a brief idea about the various DNA sequencing methods and various strategies used for genome sequencing and much more vital information related to gene expression and analysis
Comparative genomics: Genomic features are compared, evolutionary relationship
The major principle of comparative genomics is that common features of two organisms will often be encoded within the DNA that is evolutionarily conserved between them. orthologous sequences,
Started as soon as the whole genomes of two organisms became available (that is, the genomes of the bacteria Haemophilus influenzae and Mycoplasma genitalium) in 1995, comparative genomics is now a standard component of the analysis of every new genome sequence. comparative genomics studies of small model organisms (for example the model Caenorhabditis elegans and closely related Caenorhabditis briggsae) are of great importance to advance our understanding of general mechanisms of evolution
Computational tools for analyzing sequences and complete genomes. Application of comparative genomics in agriculture and medicine.
STS stands for sequence tagged site which is short DNA sequence, generally between 100 and 500 bp in length, that is easily recognizable and occurs only once in the chromosome or genome being studied.
Introduction
Transcriptome analysis
Goal of functional genomics
Why we need functional genomics
Technique
1. At DNA level
2.At RNA level
3. At protein level
4. loss of function
5. functional genomic and bioinformatics
Application
Latest research and reviews
Websites of functional genomics
Conclusions
Reference
What is Genome,Genome mapping,types of Genome mapping,linkage or genetic mapping,Physical mapping,Somatic cell hybridization
Radiation hybridization ,Fish( =fluorescence in - situ hybridization),Types of probes for FISH,applications,Molecular markers,Rflp(= Restriction fragment length polymorphism),RFLPs may have the following Applications;Advantages of rflp,disAdvantages of rflp, Rapd(=Random amplification of polymorphic DNA),Process of rapd, Difference between rflp &rapd
Genomics and its application in crop improvementKhemlata20
meaning ,definition of genome ,genomics ,tools of genomics ,what is genome sequencing ,methods of genome sequencingand genome mapping ,advantage of genomics over traditional breeding program, examples of some crops whose genome has been sequenced, important points about genomics, work in the field of genomics ,applications of genomics .classification of genomics .different Omics in genomics like Proteomics ,Transcriptomics ,Metabolomics ,Need of genome sequencing
A physical map of a chromosome or a genome that shows the physical locations of genes and other DNA sequences of interest. Physical maps are used to help scientists identify and isolate genes by positional cloning.
According to the ICSM (Intergovernmental Committee on Surveying and Mapping), there are five different types of maps: General Reference, Topographical, Thematic, Navigation Charts and Cadastral Maps and Plans.
DNA SEQUENCING METHODS AND STRATEGIES FOR GENOME SEQUENCINGPuneet Kulyana
This presentation will give you a brief idea about the various DNA sequencing methods and various strategies used for genome sequencing and much more vital information related to gene expression and analysis
Comparative genomics: Genomic features are compared, evolutionary relationship
The major principle of comparative genomics is that common features of two organisms will often be encoded within the DNA that is evolutionarily conserved between them. orthologous sequences,
Started as soon as the whole genomes of two organisms became available (that is, the genomes of the bacteria Haemophilus influenzae and Mycoplasma genitalium) in 1995, comparative genomics is now a standard component of the analysis of every new genome sequence. comparative genomics studies of small model organisms (for example the model Caenorhabditis elegans and closely related Caenorhabditis briggsae) are of great importance to advance our understanding of general mechanisms of evolution
Computational tools for analyzing sequences and complete genomes. Application of comparative genomics in agriculture and medicine.
Slides from a Comparative Genomics and Visualisation course (part 1) presented at the University of Dundee, 7th March 2014. Other materials are available at GitHub (https://github.com/widdowquinn/Teaching)
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
2. GENOMICS
■ The study of genome of an organism is called genomics.
In the field of molecular biology and genetics genome is the
genetic material of an organism.
It basically involve analysis of nucleotide sequence of genomes
using computational methods (sequencing of genome).
3. IMPORTANCE OF GENOMICS
Provides a map of the genetic make up of a cell.
• Gives an idea of how the cell responds at the
genetic level to different situations.
•This enables researchers to compare the
genomes of different organisms, to see how the
genes function in different circumstances, how
they interact, how they control over one another
or how differences in the genomes are reflected
in the phenotype.
4. BROAD COVERAGE OF GENOMICS
Structural genomics: Understanding the
content of genome and is concerned with
genome mapping, genome sequencing and
genome manipulations.
• Functional genomics: Identification of genes
and their respective functions
• Comparative genomics: Analysis and
comparison of genomes from different
species. It includes animal genomics, plant
genomics, microbial genomics etc..
5. WHAT IS COMPARATIVE GENOMICS?
Analyzing & comparing genetic material from different species
to study
■ evolution, gene function, and inherited disease
■ Understand the uniqueness between different species
6. Introduction
Comparative genomics is a large-scale, holistic approach that
compares two or more genomes to discover the similarities and
differences between the genomes and to study the biology of
the individual genomes
7. Continue…
The subject of comparative genomics impinges on
■ – Evolutionary biology and phylogenetic reconstructions of the tree of
life,
■ – Drug discovery programs,
■ – Function predictions of hypothetical proteins
■ – Identification of genes, regulatory motifs and other non-coding DNA
■ motifs
■ – Genome flux and dynamics
8. WHYWE MAKE COMPARISON
■ Comparative genomics is a field of biological research in
which the genome sequences of different species —human,
mouse, and a wide variety of other organisms from bacteria
to chimpanzees — are compared.
■ By comparing the sequences of genomes of different
organisms, researchers can understand what, at the
molecular level, distinguishes different life forms from each
other.
9. WhyThis Comparison?
■ Comparison of whole genome sequences provides a highly
detailed view of how organisms are related to each other at
the genetic level. How are genomes compared and what can
these findings tell us about how the overall structure of genes
and genomes have evolved?
10. Continue…
■ Comparative genomics also provides a powerful tool for
studying evolutionary changes among organisms, helping to
identify genes that are conserved or common among species,
as well as genes that give each organism its unique
characteristics.
12. HOW ARE GENOMES COMPARED?
A simple comparison of the general features of genomes such as
■ genome size,
■ number of genes, and
■ chromosome number presents an entry point into
comparative genomic analysis
15. Related terms..
■ Homology
■ Homologous
■ Orthologous
■ Paralogous
■ Xenologous
■ Analogoues
■ Horizontal gene transfer
16. RelatedTerms
Homology is the relationship of any two characters (such as
two proteins that have similar sequences) that have
descended, usually through divergence, from a common
ancestral character
Homologues are thus components or characters (such as
genes/proteins with similar sequences) that can be attributed
to a common ancestor of the two organisms during evolution.
Homologues can either be orthologues, paralogues, or
xenologues.
17. Continue…
Orthologues are homologues that have evolved from a common ancestral
gene by speciation.They usually have similar functions.
Paralogues are homologues that are related or produced by duplication
within a genome.They often have evolved to perform different functions.
Xenologues are homologues that are related by an interspecies
(horizontal transfer) of the genetic material for one of the homologues
Horizontal (Lateral) GeneTransfer is the movement of genetic material
between species (or genus) other than by vertical descent.
19. Methods for comparative genomics
1. Comparative analysis of genome structure
2. Comparative analysis of coding regions
3. Comparative analysis of non-coding regions
20. Comparative analysis of genome
structure
A. Analysis of the global structure of genomes, such as nucleotide
composition, and gene ordering offer insight
into the similarities and differences between genomes.
This provide information on the organization and evolution of the
genomes, and highlight the unique features of individual genomes
B. The structure of different genomes can be compared at three levels:
– Overall nucleotide statistics,
– Genome structure at DNA level.
21. Comparison of overall nucleotide
statistics
Overall nucleotide statistics, such as
– Genome size,
– Overall (G+C) content,
– Regions of different (G+C) content,
– Genome signature such as codon usage biases,
– Amino acid usage biases, and the ratio of observed dinucleotide
frequency and
–The expected frequency given random nucleotide
distribution.
22. Comparison of genome structure at DNA
level
Chromosomal breakage and exchange of chromosomal
fragments are common mode of gene evolution.They can be
studied by comparing genome structures at DNA level.
– Identification of conserved regions and genome rearrangement events
– Analysis of breakpoints
– Analysis of content and distribution of DNA repeats
23. Comparison of genome structure at
gene level
Chromosomal breakage and exchange of chromosomal
fragments cause disruption of gene orderTherefore gene order
correlates with evolutionary distance between genomes
24. Comparative analysis of coding regions
I. Identification of gene-coding regions
II. comparison of gene content
III. comparison of protein content
IV. Comparative genome based function prediction
Number of algorithms that have been use in comparative
genomics to aid function prediction of genes.
25. Comparative Genomics in Drug
Discovery
1. Comparative genomic studies throw important light on the
pathogenesis of organisms, throwing up opportunities for
therapeutic intervention as well as help in understanding and
identifying disease genes
2. One of the most important fallouts of comparative analyses at a
genome-wide scale is in the ability to identify and develop novel
drug targets
26. Continue…
3. If one is looking for antibacterial, antifungal, or antiprotozoal
proteins to be used as targets, comparative genome analysis can
reveal virulence genes, uncharacterized essential genes, species-specific
genes, organism-specific genes, while ensuring that the
chosen genes have no homologues in humans
27. Looking Beyond…
As comparative genomics moves from between kingdoms to
between genus to between species analysis, the next step is to
carry out comparisons between individuals or strains that are
members of a particular species
This would allow us to investigate variations at the individual
level and to enable one to determine the propensity of an
individual to respond to a drug or to come down with a disease
or infection
28. IMPACT OF COMPARATIVE GENOMICS
The impact of comparative genomics will be far reaching.
For example: "The genomic revolution is
having a tremendous impact on the study of natural
variation.
This will not only help us understand biology better, but aid in our
exploitation of natural diversity for
crop improvement,
plant breeding efforts and
biodiversity conservation.
These are all important to the quality of life on earth.