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BACTERIAL CONCRETE
by
S. SAIKUMAR
CIVIL ENGINEERING
• ABSTRACT
• INTRODUCTION
• PREPARATION
• TYPES OF BACTERIA USED
• MECHANISM
• EXPERIMENTAL TESTING
• APPLICATIONS
• ADVANTAGES AND DISADVANTAGES
• CONCLUSION
AGENDAAGENDA
A NOVEL TECHNIQUE IN REMEDIATING CRACKS AND FISSURES
IN CONCRETE BY UTILIZING MICROBIOLOGICALLY INDUCED
CALCITE PRECIPITATION IS DISCUSSED. MICROBIOLOGICALLY
INDUCED CALCITE PRECIPITATION (MICP) IS A TECHNIQUE THAT
COMES UNDER A BROADER CATEGORY OF SCIENCE CALLED
BIOMINERALIZATION.
ABSTRACTABSTRACT
· Primarily 12.5g of Nutrient broth (media) is added to a 500ml conical flask containing 
distilled water.
· It is then covered with a thick cotton plug and is made air tight with paper and 
rubber band.
· It is then sterilized using a cooker for about 10-20 minutes. Now the solution is 
free from any contaminants and the solution is clear orange in colour before the 
addition of the bacteria. 
 · Later the flasks are opened up and an exactly 1ml of the bacterium is added to the 
sterilized flask and is kept in a shaker at a speed of 150- 200 rpm overnight.            
· After 24 hours the bacterial solution was found to be whitish yellow turbid 
solution. FIGURE9.2: Solution with bacteria.
PREPARATIONPREPARATION
TYPES OF BACTERIA USEDTYPES OF BACTERIA USED
There are various types of bacteria were used
in construction area
· Bacillus pasteurii
· Bacillus sphaericus
· Escherichia coli
· Bacillus subtilis
· Bacillus cohnii
· Bacillus balodurans
· Bacillus pseudofirmus
MECHANISMMECHANISM
MECHANISMMECHANISM
EXPERIMENTAL TESINGEXPERIMENTAL TESING
EXPERIMENTAL TESINGEXPERIMENTAL TESING
EXPERIMENTAL TESINGEXPERIMENTAL TESING
APPLICATIONSAPPLICATIONS
ADVANTAGES:
1. Microbial Concrete in Crack Remediation.
2. Improvement in Compressive Strength of Concrete.
3. Better Resistance towards Freeze- Thaw Attack Reduction.
4. Reduction in Permeability of Concrete.
5. Reduction in corrosion of reinforced concrete structures.
DISADVANTAGES:
1. Cost of bacterial concrete is double than conventional concrete.
2. Growth of bacteria is not good in any atmosphere and media.
3. The clay pellets holding the self-healing agent comprise 20% of the
volume of the concrete.
4. Design of mix concrete with bacteria here is no available any IS code or
other code .
ADVANTAGES AND
DISADVANTAGES
ADVANTAGES AND
DISADVANTAGES
CONCLUSIONCONCLUSION
THE PRESENCE OF BACTERIA IN DIFFERENT MEDIUMS (WATER,
PHOSPHATE-BUFFER AND UREA-CaCl2 ) INCREASED THE
RESISTANCE OF CONCRETE TOWARDS ALKALI, SUFATE, FREEZE-
THAW ATTACK AND DRYING SHRINKAGE. PHOSPHATE – BUFFER
PROVED TO BE AN EFFECTIVE MEDIUM FOR BACTERIA THAN
THE TWO MEDIUMS (WATER AND UREA - CaCl2 )
Bacterial concrete

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