Plastination is a process for long-term preservation of biological tissues that leaves them completely dry but still life-like. It involves removing water and lipids from tissues and replacing them with curable polymers like silicone or epoxy. There are three main steps - fixation in formalin, dehydration in acetone, and forced impregnation with polymer under vacuum. The polymer is then hardened to complete plastination. Whole bodies, organs, or thin cross-sectional sheets can be plastinated. Plastinated specimens are durable, pose no health risks, and are useful for teaching anatomy. However, plastination is an expensive and time-consuming process requiring specialized equipment and skills.
Plasitnation as a sub-unit of museum techniques has been helpful as gross specimen for postgraduate, undergraduate and research studies. Why is so plastination important in the 21th Century?
This slide gives you details about
1. embalming
2. museum techniques
3. principles of karyotyping
chemicals used for embalming
instruments used for embalming
embalming procedures
uses of embalming
procedures for museum techniques
procedure for storing specimens
instruments used in specimen storage
different types of jars
karyotyping definition
procedure for karyotyping
Plasitnation as a sub-unit of museum techniques has been helpful as gross specimen for postgraduate, undergraduate and research studies. Why is so plastination important in the 21th Century?
This slide gives you details about
1. embalming
2. museum techniques
3. principles of karyotyping
chemicals used for embalming
instruments used for embalming
embalming procedures
uses of embalming
procedures for museum techniques
procedure for storing specimens
instruments used in specimen storage
different types of jars
karyotyping definition
procedure for karyotyping
bone, bone maceration, defleshing, bone tagging, bone labeling, bone articulation, soft tissue removal, degreasing of bones, bone bleaching, labeling, bone articulation, Storage of bones, Cold Water Maceration Method, Hot Water Maceration Method, Bug Box Maceration Method, Enzyme Maceration Method, Chemical Maceration Method,
This presentation deals tissue processing in histopathology, the detailed of presentation given blow:
Histology, study the organization of tissues at all levels, from the whole organ down to the molecular components of cells that are found in most multicellular plants and animals.
Animal tissues are classified as epithelium, with closely spaced cells and very little intercellular space; connective tissue, with large amounts of intercellular material; muscle, specialized for contraction; and nerve, specialized for conduction of electrical impulses. Blood is also sometimes considered a separate tissue type.
Plants are composed of relatively undifferentiated tissue known as meristematic tissue; storage tissue or parenchyma; vascular tissue; photosynthetic tissue or chlorenchyma and support tissue or sclerenchyma and collenchyma.
bone, bone maceration, defleshing, bone tagging, bone labeling, bone articulation, soft tissue removal, degreasing of bones, bone bleaching, labeling, bone articulation, Storage of bones, Cold Water Maceration Method, Hot Water Maceration Method, Bug Box Maceration Method, Enzyme Maceration Method, Chemical Maceration Method,
This presentation deals tissue processing in histopathology, the detailed of presentation given blow:
Histology, study the organization of tissues at all levels, from the whole organ down to the molecular components of cells that are found in most multicellular plants and animals.
Animal tissues are classified as epithelium, with closely spaced cells and very little intercellular space; connective tissue, with large amounts of intercellular material; muscle, specialized for contraction; and nerve, specialized for conduction of electrical impulses. Blood is also sometimes considered a separate tissue type.
Plants are composed of relatively undifferentiated tissue known as meristematic tissue; storage tissue or parenchyma; vascular tissue; photosynthetic tissue or chlorenchyma and support tissue or sclerenchyma and collenchyma.
Introduction
Sterilization method
Equipment's involved in large scale sterilization
Sterilization indicators
Evaluation of efficiency of sterilization /Sterility testing
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Nutraceutical market, scope and growth: Herbal drug technology
Plastination
1. PLASTINATION
by: Dr. Ravi Kant Narayan
3rd yr JR
Department of Anatomy
Pt. B. D. Sharma PGIMS, Rohtak, Haryana, India
2. • Plastination is the method of
long term preservation of the
biological tissues with
completely visible surface and
high durability.
• It was developed by
Dr. Gunther von Hagens in
1978 at the Heidelberg
University in Germany.
3. • In this technique, the water and fat of the body are
replaced by certain polymers.
• The specimens obtained after plastination are called as
PLASTINATES.
5. • Primarily, we require a lab equipped with fire
extinguishers, explosive proof lighting system and a
freezer motor fitted outside the room.
• The room should have multiple extraction points and
should be well equipped for possible spillages.
6. • Other materials required have been classified into 2
groups: A. Chemicals B. Equipments
A. Chemicals required:
1. Formalin (≤ 10%)
2. Acetone or methyl alcohol
3. Silicone or epoxy polymer
4. Biodur S3 or S6 (hardener)
5. Water.
7. B. Equipment required:
1. Containers depending on size of specimen
2. Deep freezer, motor system to create vaccum
3. Pressure gauge to measure the pressure.
8. • After procuring, the samples are prepared properly before
undergoing the procedure to form plastinates. For example:
1. Hollow organs need to be flushed, cleaned and then fixed in a
dilated position. Dilation of hollow organs will increase the
flexibility of the organs due to the thinner wall.
9. 2. Intestinal specimens may be opened to remove
ingest, sutured closed and then dilated.
3. Ostia with strong sphincters must be held open with
appropriate sized cannulas or by tubing.
10. 4. Intravascular injection of colored silicone,
gelatin, latex or epoxy may be used to highlight
vessels, etc.
14. • Under fixation, the body is embalmed, usually in a
formaldehyde solution in order to prevent the decomposition
of the body.
• Usually 10 % formaldehyde solution may be used as a
fixative, lower percentage formalin solutions may produce
less bleaching of the specimen.
15. • Minimal fixation with low percentage of formalin and short
time duration (1-2 days) will yield a specimen which is more
flexible and more natural looking.
• Fixation of hollow organs is necessary to maintain the shape
and lumen of the organ
18. • Dehydration removes the specimen fluid at -25°C.
• In this step, tissue fluid is replaced with an organic
solvent i.e acetone.
19. • First, the specimens are washed in running tap water for
two days with the aim of neutralizing the
formalin/preservative fumes during dissection.
• Tissue water and lipids were removed by subjecting the
specimens to at least three changes of acetone bath at one-
week interval in every change.
20. • The specimens were turned/agitated at least once a day
so as to ensure maximum action of the acetone on the
specimens.
• Acetone turns yellow when fats are removed.
21. • Degreasing would be considered complete when the
acetone bath remains clear.
• Acetone is used in most cases because, acetone also
serves as the intermediary solvent during the next step
of forced impregnation and it can be recycled.
22. • Acetone also helps in removal of fat at room
temperature of 20° to 25°C.
• An acetone amount of 10 times the specimen weight is
best for good results.
25. • Equipments:
1. Deep freezer (explosion proof or motor and compressor
removed and placed in a different room); Vacuum
chamber,
26. 3. Vacuum pump with pressure gauge (Vacuum is complete when the pressure is
around 5 mm Hg)
27. • This is the central step, where the intermediary solvent
(acetone) is replaced with a curable polymer such as
silicone, epoxy resin, polyester resin, etc under applied
vaccum.
28. • The dehydrated specimen is placed in a bath containing
liquid polymer.
• After some days of immersion, vacuum is applied to it.
29. • Vacuum is increased gradually to boil the intermediary
solvent (acetone), which has a lower boiling point
(+56 ° C) out of the specimen.
• Impregnation is monitored by watching the formation
of bubble on the surface of the mixture. Absence of
bubbles indicates completion of the procedure.
33. • Finally, the polymer inside the specimen has to be cured
(hardened).
• This is achieved by exposing the impregnated specimen
to a hardener which can be liquid (S3) or gaseous (S6) in
nature .
34. • S6 is a liquid that vaporizes at room temperature and
causes fast curing.
• The impregnated specimen and a bowl filled with curing
agent is placed in a tightly closed chamber for several
weeks.
35. • To enhance the curing procedure air may be bubbled
through the fluid.
• For complete curing, the specimen should be kept in a
plastic bag for several weeks.
36. • The hardener commences end to end-linkage and hence
elongation of the silicone molecules, which produces
increased viscosity of the reaction mixture.
• This linkage is reported to enhance flexibility of the
impregnated specimen
38. • Curing methods have potential problems and/or
disadvantages.
1. A white precipitate may appear on the specimen.
2. The specimen may shrink.
3. Oozing polymer may coat the specimen
39. • To avoid precipitations:
1. Use a desiccant, e.g. calcium chloride.
2. Pour the fluid gas cure into the dish and then place
the specimens into the gas chamber.
40. 3. Use slow curing, because precipitates hardly ever
form.
4. Decrease exposure time to the S6 vapour and/or
allow the excess S6 to evaporate from the cured
specimen.
41. • To avoid shrinkage:
1. Wrap the specimen with thin foil which will adhere
to the surface of the specimen.
2. Use slow cure only on specimens which have been
formalin-fixed for a prolonged period
44. Whole body / organ plastination
• In this process entire body or an organ is
plastinated.
• Total structure and relationships of an organ/body
are preserved.
48. • It is done for hollow organs like lungs, stomach, intestine,
ventricles of brain, vascular pattern of heart and kidneys.
• Specimens are dilated/ inflated during fixation,
dehydration and curing. Beautiful and precise bronchial
pattern can be seen by this technique.
Luminal plastination
49.
50. • It involves making of thin transparent or thick opaque
sections of body or an organ.
• These sheets are portable and display cross sectional
anatomy comparable to CT or MRI scan sections.
• Sheets can be made in various planes.
Sheet plastination
51. • Thin sections (1-2mm) correlates well with routine
histology slides.
• Polymers such as epoxy, polyester or polypropylene
(araldite) resins can be used for making sheet plastinates.
Sheet plastination
55. 1. The specimens are dry, easy to handle, store, transport
and long lasting.
2. There will be no formalin fume irritation on the dry
specimens (devoid of harmful effects of formalin
exposure).
56. 3. Plastination can accommodate a variety of specimens
from gross specimens to cross section slices &
therefore, can be used as an ideal alternative method of
specimen preparation for teaching and research
purposes.
58. 1. Costly procedure
2. Time consuming
3. Requires skilled technical support to carry out
the procedures and in handling the equipment.
59. 4. Prepared specimen requires handling with care.
5.Chemicals used, such as acetone are highly inflammable
and should be used in places equipped with fire
extinguishing measures.
60. REFRENCES
• Gunther von Hagens' BODY WORLDS, Institute for Plastination,
Heidelberg, Germany, www.bodyworlds.com.
• Holiaday SD, Blaylock BL, Smith BJ. Risk factors associated with Plastination:
Chemical toxicity considerations. 2001, J Int Soc Plast16:9-13.
• Hagens GV, Tiedmann K, Kriz W. The current potential of plastination. Anat
Embryol (1987) 175:411-21.
• Sargon MF, Tatar I. Plastination: basic principles and methodology. Anatomy
2014;8:13–8.
• Dhanwate AD, Gaikwad MD. Plastination – A Boon to Medical Teaching &
Research. IJSR 2015;4(5):1550-3.