Partition Chromatography technique is defined as. the separation of components between two liquid phases viz original solvent and the film of solvent used in the column.
This Powerpoint presentation helps us to know the basic working principles, instrumentation an advantage of super critical fluid chromatography.
Contact Details:
Anbu Dinesh Jayakumar
M.Pharmacy ( Pharmaceutical Chemistry)
Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore
Mobile : 8838404664 / 8608890121( Whatsapp)
Email: anbudinesh007@gmail.com
Partition Chromatography technique is defined as. the separation of components between two liquid phases viz original solvent and the film of solvent used in the column.
This Powerpoint presentation helps us to know the basic working principles, instrumentation an advantage of super critical fluid chromatography.
Contact Details:
Anbu Dinesh Jayakumar
M.Pharmacy ( Pharmaceutical Chemistry)
Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore
Mobile : 8838404664 / 8608890121( Whatsapp)
Email: anbudinesh007@gmail.com
Instrumentation of Thin Layer ChromatographyTanmoy Sarkar
Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a system on which a material called the stationary phase is fixed.
Thin-layer chromatography is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of an inert substrate such as glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose.
Column Chromatography: basics of chromatography and principle of chromatography, Classification, partition coefficient , chromatogram, retention time and volume, capacity and selectivity factors, plate theory, band broadening, rate theory, mass transfer, packed GLC column, open tubular column capillary columns, liquid chromatography column resolution.
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Instrumentation of Thin Layer ChromatographyTanmoy Sarkar
Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a system on which a material called the stationary phase is fixed.
Thin-layer chromatography is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of an inert substrate such as glass, plastic, or aluminium foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminium oxide, or cellulose.
Column Chromatography: basics of chromatography and principle of chromatography, Classification, partition coefficient , chromatogram, retention time and volume, capacity and selectivity factors, plate theory, band broadening, rate theory, mass transfer, packed GLC column, open tubular column capillary columns, liquid chromatography column resolution.
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Preeti Choudhary
The present slide gives us an insight into the different aspects of application of columnn chromatrography the principle behind it and at the same time its recent advances.
Chromatography is a powerful and advanced techniques for separating mixtures. Many types of chromatographic techniques are known, such as paper, thin layer, column chromatography, each with its own strength and weakness.
Chromatography system in general have a stationary phase and a mobile phase.
In column chromatography both phases are placed in a column container, i.e. all the chromatographic operations are carried out using column.
Column chromatography in chemistry is a method using for the Identification, separation and purify individual chemical compounds from mixtures of compounds in the large amount.
Column Chromatography is a separation technique in which components of mixture is separated by using a glass column packed with stationary phase and liquid mobile phase flowing continuously through the column.
It is suitable for the physical separation of gram quantities of material. A solvent acts as the mobile phase while a finely divided solid surface acts as the stationary phase.
Usually a glass tube with a diameter from 1cm to 10cm and a height of 20 cm to 50cm with a tap at the bottom, is used for this purpose.
Depending upon the flow of solvent down, column chromatography may be separated into two categories.
Gravity column chromatography
If the solvent is allowed to flow down the column by Gravity, or downward process, it is known as gravity column chromatography .
Flash chromatography
If the solvent is forced down the column by positive air pressure , it is called flash chromatography.
People’s Commune and Great Leap ForwardFaruk Hossen
People’s Commune and Great Leap Forward, Introduction To People’s Commune,Introduction to Great leap forward,Result of great leap forward,Result of great leap forward,History of people’s commune
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
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1. Column chromatography
COLUMN CHROMATOGRAPHY | INTRODUCTION
It is a technique employed for separation of components of mixture by continuous
distribution of components between two phases i.e. one phase moves (mobile)over
the other(stationary) in a continuous manner. when chromatography is carried out in
column it is called column chromatography.
COLUMN CHROMATOGRAPHY | PRINCIPLE
when a mixture of components dissolved in the mobile phase is introduced
into the column, the individual components move with different rates
depending upon their relative affinities.
The compound with lesser affinity towards stationary phase moves faster and
it is eluted out of the column first. The one with greater affinity towards
stationary phase moves slower down the column and hence it is eluted latter.
Thus the compounds are separated.
COLUMN CHROMATOGRAPHY | TYPES
Gravity columns:
The mobile phase move through the stationary phase by gravity.
Flash column:
The mobile phase is pushed by stream of air or nitrogen using special
adaptor.
Pumped column:
The movement of mobile phase is accelerated by using pumps that
generate low or medium pressure.
Vacuum column:
The adsorbentis applied drying into sintered glass funnel. Thesample
is applied by drying method or as solution.
Then the mobile phase is added portion by portion and vacuum is
applied after each portion to collect each fraction.
High pressure columns:
High pressure pumps are used to push the solvent through the column
which in this case must be made of stainless steel.
2. Ideal properties of stationary phase:
Particles should have uniform size and spherical shape [60-200]
microns.
Should have high mechanical stability, inert, insoluble in mobile
phase used.
Colorless
Should allow free flow of mobile phase.
Freely available and in expensive.
Preparation of stationary phase:
Adsorbents require activation before use.
This can be achieved by heating, where adsorbent losses
water and other adsorbed materials.
Generally there is an optimum temperature for activation.
Alumina -400o C silica gel- 100o C for 1hr.
Long time heating leads to loss of its activity.
Mobile phase:
To introduce the mixture into column as solvent.
To develop the zones for separation as developing agent.
To remove pure component out of the column as eluent.
Different mobile phases used: Ex; In increasing order of polarity or elution
strength.
Cyclohexane < carbon disulphide< ether < Benzene < toluene < esters <
alcohols < chloroform < acetone <water < pyridine < organic acids.
Packing of column:
The column must be packed as uniformly as possible to
minimize the distortion of the chromatographic boundaries.
Channeling is usually caused by the inclusion of air bubbles
during packing.
Types of packing:
DRY PACKING :In this the required quantity of adsorbent is poured as fine dry
powder in the column and the solvent is allowed to flow through the column till
equilibrium is reached.
3. DEMERITS:
Air bubbles are entrapped between mobile phase and stationary phase and the
column may not be uniformly packed.
Cracks appear in the adsorbent present in the column. Hence flow
characteristics and clear band of the separated component may not be
obtained.
WET PACKING:
This is the ideal technique.
The slurry of adsorbent with the mobile phase is prepared and is poured into
the column.
The stationary phase settles uniformly in the column and there is no
entrapment of air bubbles.
There will not be any crack in the column of adsorbent.
The bands eluted from the column will be uniform and ideal for separation.
Introduction of the Sample:
• The sample which is usually a mixture of components is dissolved in minimum
quantity of the mobile phase.
• The entire sample is introduced into the column at onceand get adsorbed onthe
top portion of the column.
• From this zone, individual sample can be separated by a process of elution.
Elusion:
After the introduction of the sample, by the elution techniques the individual
components are separated out from the column.
Two types of elution techniques.
Isocratic elution technique:
Iso means same/ similar. In this elution technique, the same solvent or solvent
system of same polarity is used throughout the process of separation. Ex-
chloroform only, petroleum: ether=1:1
Gradients:
4. The solvents of gradually increasing polarity or increasing elution strength are
used during the process ofseparation. Initially low polar solvent is used followed
by gradually increasing the polarity.
Ex-Initially Benzene than Chloroform, Ethyl acetate, Methanol etc.
COLUMN CHROMATOGRAPHY | DETECTION OF COMPONENTS
• If the compounds separated in a column chromatography procedureare colored,
the progress of the separation can simply be monitored visually.
• If the compounds to be isolated from column chromatography are colorless. In
this case, small fractions of the eluent are collected sequentially in labelled tubes
and the composition of each fraction is analyzed by TLC.
FACTORS AFFECTING
1. Dimension ofthe column: column efficiency has been improved by increasing
length/width ratio of the column.
2. Particle size of column packing: separation to be improved by decreasing the
particle size of the adsorbent.
3. Activity of the adsorbent
4. Temperature of the column: The speed of the elution increases at higher
temperatures.
5. Packing of the column
6. Quality of solvents: solvents having low viscosities is giving better results.
COLUMN CHROMATOGRAPHY | APPLICATIONS
►Separation of mixture of compounds
►Purification process
►Isolation of active constituents
►Estimation of drugs in formulation
►Isolation of active constituents
►Determination of primary and secondary glycosides in digitalis leaf.
► separation of diastereomers
5. COLUMN CHROMATOGRAPHY
Advantages of C.C
Any type of mixture can be separated
Any quantity of mixture can be separated
Wider choice of Mobile Phase
Automation is possible
Disadvantages of C.C
Time consuming
more amount of Mobile Phase are required
Automation makes the techniques more complicated & expensive.
CONCLUSION:
Column chromatography is a conventional tool for separation of phytochemicals,
removal of impurities and purification of drugs. Effective separation of constituents
from different sources in preparative scale (milligram to gram) can be achieved by
column chromatography. Understanding the basic principles of column
chromatography enables us to find solutions for current research problems.