This document outlines an introduction to applications of proteomics in microbiology. It discusses how proteomic techniques like MALDI-TOF can help understand host-pathogen interactions and identify microorganisms to diagnose infectious diseases. Specifically, it explains that proteomics can identify virulence proteins and post-translational modifications involved in infection. It also discusses how proteomics can be used to identify differentially expressed proteins in diseases like cancer to develop new drug therapies and identify antigenic proteins for vaccine development.

1. Applications of proteomics in
microbiology

2. Outline of lecture
 Introduction
 Understanding Host-Pathogen interactions and
infectious diseases.
 Proteomics techniques
 Role of MALDI-TOF in diagnosis
 Role of Proteomics in Medical Microbiological
Research and Diagnosis
 Research areas in medical microbiology that
utilize proteomic technologies
 Role of Proteomics in development of therapy
and vaccine

3. Introduction
 Microbial is a leading cause of death around the
world. Therefore understanding of microbial
infection and defense system and survival
strategies is of great interest in order to defeat
microbial infection.
 Microbial proteomics helps to identify the
proteins associated with microbial host-
pathogen interactions and antimicrobial
resistant mechanism by using 2-D gel based and
gel free method with combination of MALDI-
TOF-LC

4. Shotgun Proteomic Analysis

5. Understanding Host-Pathogen
interactions and infectious diseases
 Provide information about interaction
between host and pathogen.
 Proteomic profiling provides new insights of
virulence proteins like proteoglycans.
 Host-pathogen interactions of microbes will
affect posttranslational modifications like
phosphorylation,glycosylation and
acetylation identified by MS.

6. Proteomic Techniques
1. Chemical cross-linking MS
2. Proximately dependent labeling strategies
MS
3. Yeast 2 hybrid
4. Affinity purification MS(AP-MS)
5. Protein Correlation profiling

7. Role of MALDI-TOF in diagnosis
 It can be used for early identification of
bacteria in blood, urinary tract infection and
other clinical samples.
 MALDI-TOF MS is most emerging tool
used to identify microorganisms at special
level.
 Process involves different steps as
i. Protein separation
ii. 2D gel analysis
iii. Gel digestion and analysis

9. Role of Proteomics in Medical
Microbiological Research and Diagnosis
 It is used to identify differentially expressed
cancer cells.
 Proteomics has identified proteins involved
in metastasis and multidrug resistance that
are highly expressed in glioblastoma
multiform compared with brain tissue.
 Proteomics shows solution for drug
development against the resistance. For
example chloroquine.

10. Research areas in medical
microbiology that utilize proteomic
technologies

11. Role of Proteomics in development of
therapy and vaccine
 Antigenic proteins can be identified by an
immune response against the infection; these
proteins can be used in the development of
vaccine.
 Pseudomonas aeruginosa develops resistant
against beta-lactam antibiotics; proteomics
can identify the resistance mechanism; it
reduces the expression of outer membrane
protein.

12. Thank You