The document outlines the plans and objectives for AP Biology lessons during the week of January 25th through January 29th, 2016. The schedule includes notes on chapters 13 and 14 about transcription and translation on different days. Students are assigned to study figure 14.24 and there will be a quiz on transcription on February 1st. The document then reviews key concepts about transcription, including the three stages of initiation, elongation, and termination. It describes differences between transcription in prokaryotes and eukaryotes, and how pre-mRNA is modified through capping, polyadenylation, and splicing before becoming functional mRNA in eukaryotic cells.
KEY CONCEPTS
18.1 Bacteria often respond to environmental change by
regulating transcription
18.2 Eukaryotic gene expression is regulated at many stages
18.3 Noncoding RNAs play multiple roles in controlling gene
expression
18.4 A program of differential gene expression leads to the different cell types in a multicellular organism
18.5 Cancer results from genetic changes that affect cell cycle control
KEY CONCEPTS
18.1 Bacteria often respond to environmental change by
regulating transcription
18.2 Eukaryotic gene expression is regulated at many stages
18.3 Noncoding RNAs play multiple roles in controlling gene
expression
18.4 A program of differential gene expression leads to the different cell types in a multicellular organism
18.5 Cancer results from genetic changes that affect cell cycle control
Protein synthesis is the process whereby biological cells generate new proteins. Translation, the assembly of amino acids by ribosomes, is an essential part of the biosynthetic pathway, along with generation of messenger RNA (mRNA), aminoacylation of transfer RNA (tRNA), co-translational transport, and post-translational modification. Protein biosynthesis is strictly regulated at multiple steps. They are principally during transcription (phenomenon of RNA synthesis from DNA template) and translation (phenomenon of amino acid assembly from RNA). The cistron DNA is transcribed into the first of a series of RNA intermediates. The last version is used as a template in synthesis of a polypeptide chain. Protein will often be synthesized directly from genes by translating mRNA. A proprotein is an inactive protein containing one or more inhibitory peptides that can be activated when the inhibitory sequence is removed by proteolysis during post translational modification. A preprotein is a form that contains a signal sequence (an N-terminal signal peptide) that specifies its insertion into or through membranes, i.e., targets them for secretion. The signal peptide is cleaved off in the endoplasmic reticulum. Preproteins have both sequences (inhibitory and signal) still present. In protein synthesis, a succession of tRNA molecules charged with appropriate amino acids are brought together with an mRNA molecule and matched up by base-pairing through the anti-codons of the tRNA with successive codons of the mRNA. The amino acids are then linked together to extend the growing protein chain, and the tRNAs, no longer carrying amino acids, are released. This whole complex of processes is carried out by the ribosome, formed of two main chains of RNA, called ribosomal RNA (rRNA), and more than 50 different proteins. The ribosome latches onto the end of an mRNA molecule and moves along it, capturing loaded tRNA molecules and joining together their amino acids to form a new protein chain.
Transcription and synthesis of different RNAs
Processing of RNA transcript
Catalytic RNA
RNA splicing and Spliceosome
Transport of RNA through nuclear pore
Translation and polypeptide synthesis
Posttranslational modification
Protein trafficking and degradation
Antibiotics and inhibition of protein synthesis.
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This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
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June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
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Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
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Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Digital Tools and AI for Teaching Learning and Research
AP Biology Ch. 14 part 1 Transcription
1. Ch. 13 & 14
Anti - opposite
Intro - within
Muta - change
-script - to write
Helic - a spiral
Semi - half
Trans - across
Feb 17?
2. AP Biology Plans for the week of January 25th
through January
29th
, 2016
• Monday 1-25-16: Ch. 14 part 1 notes
• Tuesday 1-26-16: Ch. 14 part 2 notes
• Wednesday 1-27-16: big group diagram of transcription and
translation
• Thursday & Friday 1-28 & 1-29: Review and practice questions
for Ch. 13 and 14
Homework: Study Fig 14.24 on page 287 for a diagram quiz on MONDAY 2-1-16
3. Objectives
1. Identify and describe the 3 stages
of transcription
2. Describe how pre-mRNA is
altered to become functional
mRNA in eukaryotes
5. To aid in your notetaking…
Key vocabulary terms are in
orange, bold font and underlined
6. Overview of Concepts
1.1. The information in DNA is in theThe information in DNA is in the
sequence of nucleotidessequence of nucleotides
2.2. Transcription is the production of RNATranscription is the production of RNA
1.1. There are several types of RNAThere are several types of RNA
2.2. mRNA is the transcribed code for a proteinmRNA is the transcribed code for a protein
based on the DNAbased on the DNA
3.3. mRNA is modified in eukaryotic cellsmRNA is modified in eukaryotic cells
before it is functionalbefore it is functional
1.1. The information in DNA is in theThe information in DNA is in the
sequence of nucleotidessequence of nucleotides
2.2. Transcription is the production of RNATranscription is the production of RNA
1.1. There are several types of RNAThere are several types of RNA
2.2. mRNA is the transcribed code for a proteinmRNA is the transcribed code for a protein
based on the DNAbased on the DNA
3.3. mRNA is modified in eukaryotic cellsmRNA is modified in eukaryotic cells
before it is functionalbefore it is functional
8. RNA is the bridge between DNA
& proteins
There are some key differences between DNA & RNA
DNA RNA
Sugar -
deoxyribose
Sugar - ribose
Bases - A, T, C, G Bases - A, U, C, G
Double stranded
& very long
Single stranded &
shorter
9. To go from the DNA to proteins
requires 2 major steps:
Transcription & Translation
Transcription - DNA
to RNA
Translation - RNA to
protein
We will focus onWe will focus on
Transcription todayTranscription today
11. Why can’t we just go straight
from DNA to proteins?
Having a “middle-
man” in the form of
RNA protects the all
important DNA
It is more efficient -
many copies of that
gene can be made &
used simultaneously
12. Quick Think
What is transcription and why
is it important in the
synthesis of proteins?
13. Prokaryotes vs. Eukaryotes
Prokaryotes - both
transcription & translation
occur in the cytosol, since
there is no nucleus
These processes can happen
simultaneously
Eukaryotes - transcription
in nucleus, translation in
cytosol
14. The synthesis of mRNA
Messenger RNA is made
in much the same way
that DNA is replicated
The DNA strand serves as
a template for the linking
of complementary base
pairs
15. Quick Think
If DNA polymeraseDNA polymerase adds DNA
nucleotides to make a strand of
DNA
then
What enzyme do you suppose
adds RNA nucleotides to make a
strand of RNA?
16. RNA polymerase
This enzyme separates the DNA strands
It also bonds the RNA nucleotides
together
It attaches at a special sequence of
bases on the DNA called the promoter
region
17. The stretch of DNA that gets
transcribed is called the
transcription unit
This is the region of the DNA that
contains the information for
making the protein
18. The 3 stages of
Transcription
1. Initiation
2. Elongation
3. Termination
start
build
break-off
19. Initiation
In prokaryotesprokaryotes - RNA
pol recognizes the
promoter region and
binds directly to it
In eukaryoteseukaryotes - proteins
called transcription
factors attach to
promoter 1st, then RNA
pol attaches
An important promoter
sequence in eukaryotes is
called a TATA box
It contains the bases
TATAAAA
21. Elongation
RNA pol untwists the
DNA 10-20 bases at
a time
RNA strand is made
in the 5’ to 3’
direction, with new
bases added to the 3’
end
As it gets longer, it
peels away from the
DNA chain & the DNA
double helix reforms Several RNA pol can be working onSeveral RNA pol can be working on
the same gene at the same time,the same gene at the same time,
increasing the rate of transcriptionincreasing the rate of transcription
22. Termination
In prokaryotesprokaryotes -
RNA pol goes
through a
termination
sequence,
detaches, &
releases the
transcript.
The transcript is
available for
immediate use by
the cell
23. Question for ya
What are the 3 stages of
transcription
What happens at each stage?
24. Termination in EukaryotesEukaryotes
The pre-mRNA
strand is cut off
from the growing
RNA chain
RNA pol is still
attached to the DNA
and continues to
transcribe it
The mRNA is NOT
ready for use yet
25. Quick Think
What are some differences
between transcription in
prokaryotesprokaryotes versus eukaryoteseukaryotes?
26. Modification of the mRNA in
eukaryotes
In eukaryotic cells the
mRNA is modified before it
becomes functional
This is why it is called pre-
mRNA
In general, both ends of the
pre-mRNA are altered
Some of the middle parts
may be cut out and the
remains sliced together
27. Modification #1
The 5’ cap
A modified guanine nucleotide is
added
This is called the 5’ cap
29. Why???
The 5’ cap and the 3’ tail
help to:
Export mRNA from the
nucleus
Protect mRNA from
hydrolytic enzymes
Allow ribosomes to attach
at the 5’ end
The 5’ cap and the 3’ tail
help to:
Export mRNA from the
nucleus
Protect mRNA from
hydrolytic enzymes
Allow ribosomes to attach
at the 5’ end
30. Modification # 3
RNA splicing
In the DNA and RNA
there are long non-coding
regions between coding
regions
non-coding regions =
introns
coding regions = exons
RNA splicing removes the
introns & joins the exons
31. How does it know what to cut
out?
introns recognized by
spliceosomes (an type
of RNA + proteins)
spliceosomes cut out
the introns and fuse
the exons
32. Quick Think
What are some ways the RNA is
modified before it is translated in
eukaryotic cells?
33. Ribozymes
Ribozymes are RNA
molecules that function
as enzymes in the
splicing of RNA
Their discovery
eliminated the
hypothesis that all
enzymes were proteins
34. Why introns?
Allows for alternative RNA splicing to occur
Genes can code for more than one polypeptide
depending on which segments are treated as exons
during RNA splicing
Introns increase the likelihood of crossing over
(more places for it to occur if gene is longer)
Exons from different genes may get combined
Exon shuffling can lead to new proteins,
increasing genetic variation
35. Exit Ticket
How is the functional mRNA
different from the DNA template
that was used to produce it?
Think of as many differences as
you can. I can think of at least 7
differences.
36. Types of RNA polymerase
Only 1 type in
prokaryotes
Three types in
eukaryotes
RNA pol II is used
in the synthesis of
mRNA
RNA
polymerase
37. Termination in EukaryotesEukaryotes
The pre-mRNA
strand is cut off
from the growing
RNA chain
RNA pol is still
attached to the DNA
and continues to
transcribe it
RNA pol continues
much further down
the DNA and
eventually falls off