The document describes the anatomy of roots by detailing the 6 main anatomical characteristics:
1) The root cap protects the root tip and controls growth.
2) The epidermis contains root hairs that absorb water.
3) The cortex contains parenchyma and intercellular spaces.
4) The endodermis has a Casparian strip that regulates mineral transport.
5) The pericycle produces lateral roots and vascular tissues.
6) The vascular system contains xylem and phloem arranged in arcs alternating around the pith or core.
Xerophytes are plants which grow in xeric environment. They have adapted morphological, physiological and anatomical changes in order to survive in xeric conditions. Various anatomical adaptations in xerophytic plants which helps to absorb as much as water as possible, to store for long time and to reduce the rate of transpiration which enables them to survive in xeric condition are included in the presentation.
It explains about what is plant tissue & both the types i.e meristem & permanent tissue. It also explains about the general characteristic, and how it has been classified based on origin, position, function and plane. It also furnish further information regarding the above
Xerophytes are plants which grow in xeric environment. They have adapted morphological, physiological and anatomical changes in order to survive in xeric conditions. Various anatomical adaptations in xerophytic plants which helps to absorb as much as water as possible, to store for long time and to reduce the rate of transpiration which enables them to survive in xeric condition are included in the presentation.
It explains about what is plant tissue & both the types i.e meristem & permanent tissue. It also explains about the general characteristic, and how it has been classified based on origin, position, function and plane. It also furnish further information regarding the above
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
1. ANATOMY OF ROOT
By
Dr. Gunmala Gugalia
Associate Professor
Dept. of Botany, Sangam University
Bhilwara, Rajasthan(India)
2. SIX ANATOMICAL CHARACTERISTICS OF THE
ROOT.
The characteristics
are: 1. Root Cap
2. Epidermis
3. Cortex
4. Endodermis
5. Pericycle
6. Vascular System.
3. Root: Anatomical Characteristic #
1. Root Cap:
The root cap consists of parenchymatous cells in various
stages of differentiation. It is protective in function.
The root cap is apparently the site of the perception of gravity;
thus it appears to be capable of controlling the production in
the meristem of the growth- regulating substances involved in
geotropism or their movement.
2. Epidermis:The epidermis is also known as epiblema or
piliferous layer. In most of roots, root hairs develop from some
of the epidermal cells at a little distance from the apical
meristem
4.
5. 3. Cortex:
In most roots the cortex is parenchymatous.
In some roots, the cells of the cortex are very regularly
arranged, both radially and in concentric circles.
Conspicuous intercellular spaces may be present, and
especially evident in aquatic species, where they form a type of
aerenchyma.
The cortical cells often contain starch, and sometimes
crystals. Sclerenchyma is more common in the roots of
monocotyledons than those of dicotyledons.
The innermost layer of the cortex is usually differentiated as
an endodermis.
6. 4. Endodermis:
The endodermis comprises a single layer of cells
differing physiologically and in structure and function from
those on either side of it.
In the young endodermal cells a band of suberin,
Casparian strip, runs radially around the cell and is
thus seen in the radial walls in transverse sections of
roots.
The thin-walled passage cells often remain in the
endodermis in positions opposite the protoxylem which is
known as passage cells
8. 5. Pericycle:
The pericycle is usually a single layer of
parenchymatous cells lying just within the
endodermis and peripheral to the vascular tissues.
The pericycle has a capacity for meristematic
growth, and gives rise to lateral root primordia,
parts of the vascular cambium, and usually the
meristem which produces cork, the phellogen.
The pericycle is sometimes called
pericambium.
9. 6. Vascular System:
The vascular system of the root as seen in transverse section consists
of a variable number of triangular rays of thick-walled, lignified
tracheary elements, alternating with arcs of thin-walled phloem.
In the root, the xylem and phloem do not lie on the same radius.
The xylem may form a solid central core, or there may be a
parenchymatous or sclerenchymatous pith, as in the roots of many
monocotyledons.
Roots with 1, 2, 3, 4, 5 and many arcs of xylem are respectively called
monarch, diarch, triarch, tetrarch, pentarch and polyarch.
The xylem is exarch, i.e., protoxylem lies towards periphery and
metaxylem towards the centre.
10. The xylem is always centripetal in its development.
The phloem bundle consists of sieve tubes, companion cells
and phloem parenchyma.
The protoxylem consists of annular and spiral vessels and
meta-xylem of reticulate and pitted vessels.
The parenchyma found in between xylem and phloem bundles
is known as conjunctive tissue.
The pith may be large, small or altogether absent.
12. DIFFERENCE BETWEEN DICOT AND MONOCOT
ROOT
1. Cortex is comparatively narrow.
2. The epiblema, the cortex and
even the endodermis are peeled
off and replaced by cork.
3. Older root has a covering of cork.
4. Endodermis is less thickened and
casparian strips are more
prominent.
5. Passage cells are generally
absent in endodermis.
6. Pericycle produces lateral roots,
cork cambium and part of the
vascular cambium.
1. Cortex is very wide.
2. Cork is not formed. The cortex
and the endodermis persist. Only
the epiblema is peeled off.
3. Older root has a covering of
exodermis.
4. Casparian strips are visible only
in young root. The endodermal
cells later become highly
thickened.
5. Thin walled passage cells
generally occur in the
endodermis opposite the
protoxylem point.
6. Pericycle produces lateral roots
only.
Dicot Root Monocot root
13. DIFFERENCE BETWEEN DICOT AND MONOCOT
ROOT
7. The number of xylem and phloem
bundles varies from 2-5 or
sometimes 8.
8. Xylem vessels are generally
angular.
9. Conjunctive tissue is
parenchymatous.
10. Conjunctive parenchyma forms
the cambium.
11. Secondary growth takes place
with the help of vascular
cambium and cork cambium.
12. Pith is either absent or very
small.
7. Xylem and phloem bundles are
numerous and are 8 or more in
number.
8. Xylem vessels are oval or
rounded.
9. Conjunctive tissue may be
parenchymatous or
sclerenchymatous.
7. Conjunctive parenchyma does
not produce cambium.
10. Secondary growth is absent.
11. A well-developed pith is present
in the center of the root.
Dicot Root Monocot root