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Metamorphosis
• In most species of animals, embryonic
development leads to a larval stage with
characteristics different from those of adult
organisms
• Example: Pleutus larva of Sea Urchin ,
Caterpillar larva of Butterflies
• During metamorphosis developmental
processes are reactivated by certain hormones
• In Amphibian tadpole, metamorphosis causes
the developmental maturation
• Thus metamorphosis is often a time of
dramatic developmental change affecting the
entire organism
Morphological changes
• Here it prepares an aquatic organism for a
terrestial exsistence
• In urodeles and anurans
• Regressive changes and constructive process
• Means of locomotion changes as:
Paddle tail recedes, cartilagenous skull is
replaced, horny teeth are replaced, tongue
muscle develops
• Large intestine shortens, gills regress, lungs
enlarge
• Sensory appendages changes too as the lateral
line system of tadpole degenerates and eyes
and ear undergo further differentiation
• Middle ear and eyelids develops
Nervous system
• Nervous system undergoes dramatic changes
• In anuran metamorphosis the movement of
eyes forward from their originally lateral
position
• Some larval neurons in tadpole jaw, switch
their allegiances from larval muscle to newly
formed adult muscle
• Other neurons innervating the tongue first
form synapse during metamorphosis
Biochemical changes
• Pigment : In tadpoles the major retinal
pigment is porphyropsin it changes to
rhodopsin
• Haemoglobin : Adult haemoglobin binds
oxygen more slowly
• Liver enzymes change
• Tadpoles are amminotelic and adult frogs are
ureotelic
• Urea cycle enzymes are synthesized
System Larva Adult
Locomotory Aquatic; tail fins Terrestrial; tailless tetrapod
Respiratory Gills, skin, lungs; larval hemoglobins Skin, lungs; adult hemoglobins
Circulatory Aortic arches; aorta; anterior, posterior, and
common jugular veins
Carotid arch; systemic arch; cardinal
veins
Nutritional Herbivorous: long spiral gut; intestinal
symbionts; small mouth, horny jaws, labial
teeth
Carnivorous: Short gut; proteases; large
mouth with long tongue
Nervous Lack of nictitating membrane; porphyropsin,
lateral line system, Mauthner's neurons
Development of ocular muscles,
nictitating membrane, rhodopsin; loss of
lateral line system, degeneration of
Mauthner's neurons; tympanic membrane
Excretory Largely ammonia, some urea (ammonotelic) Largely urea; high activity of enzymes of
ornithine-urea cycle (ureotelic)
Integumental Thin, bilayered epidermis with thin
dermis;no mucous glands or granular glands
Stratified squamous epidermis with adult
keratins; well-developed dermis contains
mucous glands and granular glands
secreting antimicrobial peptides
Summary of some metamorphic changes in anurans
Hormonal control
• Demonstrated by Guder-Natsch (1912)
• Later by Allen (1916)
• Metamorphic changes of frog are all brought
about by secretion of the hormones
Thyroxine(T4) and Triiodothyronine(T3)
• T3 is thought to be the most important
hormone
Regionally specific changes
• The various organs of the body respond
differently to hormonal stimulation
• Example: Degeneration of tail structure
• The regression of tail is brought about by
apoptosis and occurs in 4 stages
- Protein synthesis decrease
- Increase in concentration of digestive enzymes
- Concentration of lysosomal protease rise
- Cell death occurs
If metalloproteinase inhibitor (TIMP) Is added to the tail,
it prevents tail regression
• The response to thyroid hormone is specific to
the regions of the body.
Coordination of developmental
changes
• Example: Tail should not degenerate until
limbs developed
• Model for this is called Threshold concept
• What if tadpoles are deprived of thyroid
hormones or given higher concentrations of
thyroid hormones?
• Two organs most sensitive to thyroxine are
thyroid itself and pituitary gland, which
regulates thyroid hormone production
References
• Developmental Biology, Gilbert SF (6th edition)
• http://archives.evergreen.edu
• http://www.jstor.org
Amphibian Metamorphosis

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Amphibian Metamorphosis

  • 1.
  • 2. Metamorphosis • In most species of animals, embryonic development leads to a larval stage with characteristics different from those of adult organisms • Example: Pleutus larva of Sea Urchin , Caterpillar larva of Butterflies
  • 3. • During metamorphosis developmental processes are reactivated by certain hormones • In Amphibian tadpole, metamorphosis causes the developmental maturation • Thus metamorphosis is often a time of dramatic developmental change affecting the entire organism
  • 4. Morphological changes • Here it prepares an aquatic organism for a terrestial exsistence • In urodeles and anurans • Regressive changes and constructive process
  • 5. • Means of locomotion changes as: Paddle tail recedes, cartilagenous skull is replaced, horny teeth are replaced, tongue muscle develops • Large intestine shortens, gills regress, lungs enlarge • Sensory appendages changes too as the lateral line system of tadpole degenerates and eyes and ear undergo further differentiation • Middle ear and eyelids develops
  • 6. Nervous system • Nervous system undergoes dramatic changes • In anuran metamorphosis the movement of eyes forward from their originally lateral position
  • 7. • Some larval neurons in tadpole jaw, switch their allegiances from larval muscle to newly formed adult muscle • Other neurons innervating the tongue first form synapse during metamorphosis
  • 8. Biochemical changes • Pigment : In tadpoles the major retinal pigment is porphyropsin it changes to rhodopsin • Haemoglobin : Adult haemoglobin binds oxygen more slowly • Liver enzymes change • Tadpoles are amminotelic and adult frogs are ureotelic • Urea cycle enzymes are synthesized
  • 9.
  • 10. System Larva Adult Locomotory Aquatic; tail fins Terrestrial; tailless tetrapod Respiratory Gills, skin, lungs; larval hemoglobins Skin, lungs; adult hemoglobins Circulatory Aortic arches; aorta; anterior, posterior, and common jugular veins Carotid arch; systemic arch; cardinal veins Nutritional Herbivorous: long spiral gut; intestinal symbionts; small mouth, horny jaws, labial teeth Carnivorous: Short gut; proteases; large mouth with long tongue Nervous Lack of nictitating membrane; porphyropsin, lateral line system, Mauthner's neurons Development of ocular muscles, nictitating membrane, rhodopsin; loss of lateral line system, degeneration of Mauthner's neurons; tympanic membrane Excretory Largely ammonia, some urea (ammonotelic) Largely urea; high activity of enzymes of ornithine-urea cycle (ureotelic) Integumental Thin, bilayered epidermis with thin dermis;no mucous glands or granular glands Stratified squamous epidermis with adult keratins; well-developed dermis contains mucous glands and granular glands secreting antimicrobial peptides Summary of some metamorphic changes in anurans
  • 11. Hormonal control • Demonstrated by Guder-Natsch (1912) • Later by Allen (1916)
  • 12. • Metamorphic changes of frog are all brought about by secretion of the hormones Thyroxine(T4) and Triiodothyronine(T3) • T3 is thought to be the most important hormone
  • 13. Regionally specific changes • The various organs of the body respond differently to hormonal stimulation • Example: Degeneration of tail structure • The regression of tail is brought about by apoptosis and occurs in 4 stages - Protein synthesis decrease - Increase in concentration of digestive enzymes - Concentration of lysosomal protease rise - Cell death occurs
  • 14. If metalloproteinase inhibitor (TIMP) Is added to the tail, it prevents tail regression
  • 15. • The response to thyroid hormone is specific to the regions of the body.
  • 16. Coordination of developmental changes • Example: Tail should not degenerate until limbs developed • Model for this is called Threshold concept • What if tadpoles are deprived of thyroid hormones or given higher concentrations of thyroid hormones? • Two organs most sensitive to thyroxine are thyroid itself and pituitary gland, which regulates thyroid hormone production
  • 17. References • Developmental Biology, Gilbert SF (6th edition) • http://archives.evergreen.edu • http://www.jstor.org